基于Lab色彩空间的沙尘降质图像增强方法

针对沙尘天气导致的数字图像质量大幅下降问题,提出一种基于Lab色彩空间的沙尘降质图像增强方法。将沙尘图像增强分解为偏色校正与细节增强两个步骤处理。偏色校正部分包括去除色偏与亮度拉伸。首先对Lab与YUV色彩空间中的沙尘图像直方图偏移特点进行了分析,然后提出一种Lab空间偏色校正算法修正直方图偏移,并对初步去除偏色的图像进行亮度拉伸,提升图像对比度。在细节增强部分,引入一种基于饱和度估计透射率的去雾方法进一步增强图像细节信息。实验结果表明,相较于各对比算法,所提算法可以去除不同程度沙尘带来的色偏,且在面对中小型图像时具有最佳的时间性能表现。在量化指标方面,基于无参考感知的图像质量评估标准和基于熵的无参考图像质量评价标准分别提升了3.2%和10.7%。本文方法可以有效去除色偏,还原清晰图像。

基于单片机和LabVIEW的无线温度监测与控制系统设计

本文设计了一个基于STC89C52单片机和LabVIEW的无线温度监测与控制系统.该系统采用上下位机的结构,其中下位机通过STC89C52单片机控制DS18B20采集实时温度,并利用控制模块实现加热或降温,同时,通过蓝牙模块实现与上位机的无线通信;上位机以LabVIEW作为开发平台,实现对温度的实时显示、处理、存储、阈值设定和温度控制等功能.该系统电路设计简单、成本低、扩展性强、人机界面友好,可用于智能大棚的温度控制等需要温度检测的场所,具有广阔的应用前景.

小麦蛋白水解物对嗜酸乳杆菌(Lactobacillus acidophilus)生长的影响研究

该研究对比了小麦蛋白水解物与不同蛋白胨对嗜酸乳杆菌生长的影响,重点从分子质量分布、氨基酸组成等方面解析了小麦蛋白水解物对嗜酸乳杆菌(Lactobacillus acidophilus)形态、活菌数、冻干存活率的影响。结果表明,小麦蛋白水解物是一种适用于嗜酸乳杆菌培养的有机氮源,同等培养条件下活菌数和冻干存活率高于其他蛋白胨,小麦蛋白水解物中分子质量为2000~5000 Da的组分是促进嗜酸乳杆菌生长的关键营养,含谷氨酰胺、脯氨酸组成的多肽可能是促进嗜酸乳杆菌缩短菌体,活菌数和冻干存活率提高的关键因素。该研究结果可为小麦蛋白水解物在嗜酸乳杆菌生产及其他发酵领域的应用提供一定的基础数据支撑。

羊源Lactobacillus plantarum对腹泻羔羊空肠菌群及肠道黏膜屏障的调控作用

旨在研究Lactobacillus plantarum对腹泻羔羊血清抗氧化能力和空肠菌群及空肠黏膜屏障损伤的调控作用。本试验选用24只刚断奶的羔羊,采用完全随机试验设计随机分为4组,分别为对照组(C组)、腹泻组(D组)、腹泻+抗生素处理组(DA组)、腹泻+植物乳杆菌处理组(DL组),每组6个重复,在正式试验第1天开始对D组、DA组和DL组羔羊分别灌服20 mL ETEC K99菌液,持续8 d。在第9天早上,DA组羔羊注射氟苯尼考,DL组羔羊灌服植物乳杆菌菌液,持续4 d。试验第1、9、13天空腹采集羔羊血液用于检测羔羊血清抗氧化指标以及二胺氧化酶(DAO)和D-乳酸(D-LA)浓度;屠宰后采集羔羊空肠内容物以测定菌群组成,采集空肠组织用于检测肠道黏膜屏障相关蛋白和黏膜蛋白,阿利新蓝(AB)染色研究酸性黏液的变化。结果表明:1)大肠杆菌处理8 d后,D、DA和DL三组羔羊粪便均不成形,且三组羔羊血清中DAO和D-LA浓度显著高于C组(P<0.05),说明腹泻模型构建成功;2)在第9天,与C组相比,D、DA和DL组羔羊血清中T-AOC、SOD显著降低(P<0.05),MDA含量显著升高(P<0.05);与第9天相比,DA和DL组羔羊在第13天时其抗氧化能力显著增加(P<0.05),DAO、D-LA浓度和MDA显著降低(P<0.05);3)与C组相比,D组羔羊空肠组织Occludin、Claudin1和MUC2蛋白表达量显著下降(P<0.05);与D组相比,DA组羔羊空肠组织Occludin蛋白表达量显著提高(P<0.05),但Claudin1蛋白和MUC2蛋白的表达量有所降低(P>0.05);DL组羔羊空肠组织Occludin蛋白、Claudin1蛋白以及MUC2蛋白表达量显著高于DA组;4)与C组相比,D和DA组羔羊空肠内容物中菌群Chao值和Shannon值均显著降低(P<0.05),DL羔羊空肠内容物中菌群Chao值和Shannon值有所增加(P>0.05)。C、D和DL组羔羊空肠中Firmicutes丰度均显著高于DA组(P<0.05)。C、D和DA组Actinobacteriota丰度都显著高于DL组(P<0.05)。D组羔羊空肠中Proteobacteria丰度略高于C和DL组(P>0.05),DA组羔羊空肠中Proteobacteria丰度显著高于C、D和DL组(P<0.05)。经相关性分析发现,与MUC2表达相关的微生物较多,Proteobacteria与空肠MUC2的表达显著负相关(P<0.05),Firmicutes和Verrucomicrobiota与空肠MUC2的表达显著正相关(P<0.05),NK4A214_group与MUC2表达量显著正相关(P<0.05),Escherichia-Shigella与MUC2的表达显著负相关(P<0.05)。综上表明,ETEC K99诱发羔羊腹泻,腹泻羔羊血清抗氧化能力和空肠内容物微生物多样性降低、空肠黏膜屏障蛋白表达量降低。相比于抗生素治疗,灌服Lactobacillus plantarum增加了空肠内容物菌群多样性,促进空肠酸性黏液及黏膜组织屏障蛋白的表达。总体而言,Lactobacillus plantarum对羔羊腹泻改善效果更佳。

Amelioration of dextran sodium sulphate-induced colitis in mice by treatment with Lactobacillus rhamnosus and Lactobacillus reuteri:intraspecific and interspecific patterns

Lactobacillus rhamnosus(Rh)and Lactobacillus reuteri(Re)are well-known probiotic species in inflammatory bowel disease(IBD)research.The variations between these species’efficacy against colitis,and their model of action in this regard,are intriguing and enable treatment to be individually tailored to patients.In this study,four strains each of Rh and Re were isolated from fecal samples and their draft genomes were sequenced.The anti-colitis activities of both strains involved various aspects of intestinal immune,physical,chemical,and biological barrier function.Strikingly,the tested strains exhibited considerable interspecies and intraspecies specificity in colitis amelioration.Rh strains significantly outperformed Re strains in terms of short-chain fatty acid synthesis.Nevertheless,Re strains were more effective than Rh strains in inhibiting production of inflammatory factors;promoting production of intestinal mucus,antimicrobial peptides,and tight junction proteins;and supporting the stem cell compartment.This accounts for the anti-colitis outcomes of Re strains being superior to those of Rh strains.In addition,the effective Rh and Re strains were found to express high concentrations of specific carbohydrate metabolism-and prophage-related genes,respectively.Taken together,the results of this study could assist researchers in developing effective therapies for IBD.

Antiseptic Efficacy of A Soap Made from Biosurfactants Isolated from Bacillus and Lactobacillus against Pathogenic Bacteria

The aim of our study was to use a biosurfactant produced by Bacillus and Lactobacillus isolates as an antiseptic in the formulation of local soap. A total of 60 isolates were characterized by microbiological techniques (30 Bacillus and 30 Lactobacillus) and the ability to produce biosurfactants was demonstrated by a hydrocarbon emulsification index (E24). The emulsification indexes (E24) varied from 9% to 100% for Bacillus and from 33% to 100% for Lactobacillus as well. The antagonistic assay showed that biosurfactants were able to inhibit the formation of biofilms and growth of pathogens such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Salmonella typhirium, Shigella boydii and Proteus mirabilis. The biosurfactant consortium (BioC) from Bacillus consortium and from Lactobacillus was able to inhibit biofilm formation and the pathogens growth. The BioC was stable to alkaline pH and the temperatures stability of Biosurfactant was ranging from 50°C to 90°C. The soap was made by the cold saponification process using one biosurfactant consortium formulated. This soap has a pH of 10 and showed good cleaning power and good foam stability. Similarly, the soap showed good antiseptic power and disinfection power against all pathogens tested. Handwashing is critical to preventing disease transmission. The persistence of pathogens in waste water was evaluated. The BioS produced showed good disinfection power against all pathogens tested. The valor of reduction on the hands and in the waste water was significantly more than compared to the control soaps used. This soap could be used in the prevention, fighting, and treatment of bacterial and viral infections.

Impact of Dietary Lactobacillus plantarum Postbiotics on the Performance of Layer Hens under Heat Stress Conditions

This experiment was conducted to determine the performance of heat-stressed layers fed a diet containing the probiotic Lactobacillus plantarum RS5 or its products of fermentation (postbiotics). Twenty-week-old Isa White layers, were subdivided into six treatments of 32 individually caged birds. Half of the birds were reared under regular temperature conditions, while the other half was subjected to cyclic daily heat stress. Layers were offered one of three diets: 1) Control;2) Control + Lactobacillus plantarum RS5 probiotic;3) Control + Lactobacillus plantarum RS5 postbiotics. Birds were tested for performance and visceral organ development for 5 months. Heat stress negatively affected the birds’ feed intake, egg weight, shell weight percentage, Haugh unit, shell thickness, yolk color, body weight and spleen weight percentage. Postbiotics significantly increased egg production (p < 0.05) in comparison to the control and the probiotic fed group (94.8% vs 92.6% vs 93.1%, respectively). Birds under probiotic or postbiotic diet showed a significantly higher (p < 0.05) feed intake and egg weight, although the probiotic had a more pronounced and gradual effect. Specific gravity, yolk weight percentage and shell thickness didn’t show differences among dietary groups. The Haugh Unit was significantly higher (p < 0.05) in probiotic group which also showed a significantly lower yolk color index (p < 0.05). The different feed treatments did not impact the bird’s viscera weight percentage, except for the ileum that was significantly lower (p < 0.05) under postbiotic supplementation. Both probiotics and postbiotics could be used as a potential growth promoters and might alleviate heat stress impact in poultry industry.

没食子酸对植物乳杆菌Lactobacillus plantarum的抑制和促进生长

该文以微生物生长曲线、多酚浓度变化和培养液pH值变化为指标,研究了没食子酸(GallicAcid,GA)和表没食子儿茶素没食子酸酯(Epigallocatechin Gallate,EGCG)对植物乳杆菌CICC 6253生长的影响。结果表明GA和EGCG对植物乳杆菌生长的影响具有浓度依赖性,可表现出抑制和促进的双向调节作用,且二者的作用存在构效差异性。在GA和EGCG分别在5 mg/mL和7 mg/mL以上时,两者都抑制了植物乳杆菌的生长,而当GA和EGCG分别低于4 mg/mL和6 mg/mL时二者都促进了细胞的增殖,且随质量浓度增加其促进作用增大。GA可作为碳源被植物乳杆菌迅速利用,而EGCG(4 mg/mL)因其良好的细胞亲和性表现出显著的促进作用,高效地促进了植物乳杆菌细胞的增殖,菌落数在28h内由7.42lgCFU/mL增加到12.54lgCFU/mL,与空白对照组相比增加了2个数量级以上。本研究表明,GA和EGCG均表现出对植物乳杆菌的双向调节作用,其中EGCG表现出更佳的益生特性。本文研究结果可为GA和EGCG及类似食源性多酚在益生菌生长调节和相关食品研发中提供参考。

Demonstration of safety characteristics and effects on gut microbiota of Lactobacillus gasseri HMV18

Human normal flora is a source of probiotics.The safety characteristics of a specific isolate determine its application in foods or drugs.The food-borne-pathogen antagonist strain Lactobacillus gasseri HMV18 is one of the isolates from normal human flora.In this work,we assessed the in vitro pH tolerance,bile tolerance,biogenic amine production,mucin utilization,and safety of in vivo administration to mice to evaluate general health,organ-body weight index,organ histopathological change,whether L.gasseri HMV18 can colonize in the gut or modulate the gut microbiota after oral administration.The results suggest that L.gasseri HMV18 can tolerate pH 3 for 2 h,3%bile for 3 h,biogenic amine negative,mucin usage negative,does not encode verified toxins,and cause no visible change in mice's organs.L.gasseri HMV18 might not colonize in mice's gut,but can significantly affect the structure of gut microbiota.A bibliographical survey suggested that there were as few as 8 opportunistic infection cases from 1984 to 2022 and that the possibility for L.gasseri to cause infection is relatively low.Therefore,this work provides a basis for the foods or drugs application of L.gasseri HMV18 and gives a map of experiments for the safety assessment of probiotics.

Identification,Characterization,and Probiotic Potentials of Lactobacillus pentosus SF-1

In recent years probiotics have been considered as a potential substitution of antibiotics to control pathogens and treat infectious diseases in aquaculture.In the present study a strain of Lactobacillus pentosus,named as L.pentosus SF-1,was isolated from waters in aquaculture.The species identification of this strain was conducted by 16S rRNA sequence,and the physiological and biochemical characteristics of this strain were assessed.Furthermore,the virulence,antibiotic sensitivity,cell surface characteristics and acid/base-resistance of L.pentosus SF-1 were determined to evaluate the probiotic potentials of this strain.Specifically,L.pentosus SF-1 is sensitive to most common antibiotics,and no hemolysin was generated from it,indicating the safety of this strain to hosts.In addition,L.pentosus SF-1 was able to tolerate the artificial gastric juice at pH 3 for 4 h and the artificial intestinal fluid at pH 6.8 or 8.0 for 6 h.Moreover,the analysis of self-aggregation and the adhesion of L.pentosus SF-1 to organic solvents suggested a high potential of L.pentosus SF-1 to inhabit the hosts,which was confirmed by testing the colonization of L.pentosus SF-1 in germ-free zebrafish.Interestingly,L.pentosus SF-1 displayed a high bactericidal activity against several bacterial pathogens.Consistently,the incubation of L.pentosus SF-1 significantly promoted the expression of antimicrobial components in zebrafish,contributing to the protection of the fish from E.tarda infection in vivo.Taken together,the probiotic strain L.pentosus SF-1 could be applied as anti-infection reagent in aquaculture.

Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp.bulgaricus in skimmed milk

Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years,though the biodegradation mechanism and derived intermediate products remain unclear.This study aimed to help fill this knowledge gap and examined the degradation mechanism of organophosphorus pesticide,chlorpyrifos,in milk by Lactobacillus delbrueckii ssp.bulgaricus using gas chromatography-tandem mass spectrometry(GC-MS/MS)combined with transcriptome analysis.After the strain was cultured for 20 h in the presence of chlorpyrifos,differential expressions of 383 genes were detected,including genes probably implicated during chlorpyrifos degradation such as those related to hydrolase,phosphoesterase,diphosphatase,oxidoreductase,dehydratase,as well as membrane transporters.GC-MS/MS analysis revealed the changes of secondary metabolites in L.bulgaricus during milk fermentation due to chlorpyrifos stress.6-Methylhexahydro-2H-azepin-2-one,2,6-dihydroxypyridine and methyl 2-aminooxy-4-methylpentanoate as intermediates,along with the proposed pathways,might be involved in chlorpyrifos biodegradation by L.bulgaricus.

基于转录组学探究Lactobacillus plantarum 6A促进二乙基亚硝胺形成的机制

乳酸菌是食品工业和人体肠道常见微生物,可促进强致癌物亚硝胺的合成,而其促进机制尚待揭示,严重制约了发酵食品安全的提升和益生菌产品的推广。该文考察植物乳杆菌(Lactobacillus plantarum)6A对二乙基亚硝胺(N-nitrosodiethylamnie,NDEA)促进作用在不同环境中的变化趋势,以转录组技术进一步探究促进作用的关键基因和形成途径,构建其机制网络。结果表明,NDEA胁迫L.plantarum 6A通过上调嘌呤核苷酸合成/代谢(关键基因purL、purK、purC、purB、purA)、核黄素合成/代谢(ribBA、ribH、ribE)、双组分系统途径(dltD、dltB、dltA)的基因表达,产生NDEA前体物,如富含氮元素的嘌呤、核黄素及丙氨酸,从而促进NDEA形成;还可能通过核黄素合成/代谢以及双组分系统途径间接催化亚硝化反应,加速NDEA的形成。

嗜酸乳杆菌(Lactobacillus acidophilus)合成纳米硒特性研究

乳酸菌具有还原亚硒酸钠为纳米硒的能力。为了采用更加环境友好的方式获得纳米硒颗粒,本研究首先探索了培养基中加硒浓度、加硒时间,以及培养时长对嗜酸乳杆菌(Lactobacillus acidophilus)合成纳米硒的影响,随后对所产的纳米硒颗粒的粒径、zeta电位及抑菌活性进行研究。结果表明,当培养基中硒含量为0~600μg/mL时,硒浓度越高,转化得到的纳米硒越多;当培养基中硒浓度高于600μg/mL时,发酵液中的纳米硒含量反而减少。在嗜酸乳杆菌生长的对数中前期,即第6 h时添加亚硒酸钠,更有利于纳米硒的合成。而培养至32 h后,纳米硒几乎不再继续合成。通过扫描电镜及粒径和电位仪分析发现,嗜酸乳杆菌所产的纳米硒颗粒呈球形,且超声破碎有助于其释放;纳米硒颗粒带负电荷,电位绝对值为40~50 mV,粒径大小较多分布于170~210 nm,具有良好的稳定性。抑菌实验结果表明,嗜酸乳杆菌还原亚硒酸钠所产的纳米硒对大肠杆菌、沙门氏菌、金黄色葡萄球菌和枯草芽孢杆菌的生长均具有抑制作用。综上,嗜酸乳杆菌可以将亚硒酸钠还原合成纳米硒,且所产的纳米硒颗粒具有良好的抑菌活性。

Lactobacillus from fermented bamboo shoots prevents inflammation in DSS-induced colitis mice via modulating gut microbiome and serum metabolites

Fermented bamboo shoots(FBS)is a region-specific food widely consumed in Southwestern China,with Lactobacillus as the predominant fermenting bacteria.However,the probiotic potential of Lactobacillus derived from FBS reminds largely unexplored,especially for diseases with a low prevalence in areas consuming FBS,namely,inflammatory bowel disease.In this study,Lactiplantibacillus pentosus YQ001 and Lentilactobacillus senioris YQ005 were screening by in vitro probiotic tests to further investigate the probioticlike bioactivity in dextran sulfate sodium(DSS)-induced ulcerative colitis(UC)mouse.They exhibited more positive probiotic effects than Lactobacillus rhamnosus GG in preventing intestinal inflammatory response.The results revealed that both strains improved the abundance of deficient intestinal microbiota in UC mice,including Muribaculaceae and Akkermansia.In the serum metabolome,they modulated the DSS-disturbed levels of metabolites,with significant increment of cinnamic acid.Meanwhile,they reduced the expression levels of interleukin-1β(IL-1β),interleukin-6(IL-6)inflammatory factors and increased zonula occludens-1(ZO-1),Occludin,and cathelicidin-related antimicrobial peptide(CRAMP)in the colon.Consequently,these results demonstrated that Lactobacillus spp.isolates derived from FBS showed promising probiotic activity based on the gut microbiome homeostasis modulation,anti-inflammation and intestinal barrier protection in UC mice.

Effect of dietary supplement of inactivated Lactobacillus plantarum Ep-M 17 on growth performance,immune response,disease resistance,and intestinal microbiota in Penaeus vannamei

Our previous study found that feeding with Lactobacillus plantarum Ep-M17 could effectively affect the growth performance,immune response,and gut microbiota of Penaeus vannamei.However,high temperature and pressure during feed pelletizing is the main problem that can lead to a decrease in the activity of probiotics or cause their inactivation.Further investigation needs to investigate whether inactivated Ep-M17 can exert similar effects as live Ep-M17.Therefore,we evaluated the effects of inactivated L.plantarum Ep-M17 on growth performance,immune response,disease resistance,and gut microbiota in P.vannamei.Results show that adding inactivated Ep-M17 to the feed also promoted body weight gain and increased relative immune protection in shrimp.Also,histological examination revealed that the administration of inactivated Ep-M17 led to improvements in the density and distribution of microvilli in the intestines and enhancements in the abundance of B and R cells in the hepatopancreas.Additionally,the inactivated Ep-M17 supplementation resulted in increased activity levels of nutrient immune-related enzymes in both the shrimp hepatopancreas and intestines.Moreover,it stimulated the expression of Lvlec,PEN-3a,Crustin,LGBP,Lysozyme,and proPo genes in both the hepatopancreas and intestines.Furthermore,the inactivated Ep-M17 also increased bacterial diversity in the gut of shrimp and promoted the abundance of specific flora,facilitating the host organism’s metabolism and immunity to improve the disease resistance of shrimp.Therefore,supplementation of inactivated L.plantarum Ep-M17 in shrimp diets can exert similar effects as live L.plantarum Ep-M17 effectively improving growth performance,gut microbiota,immune response,and disease resistance in P.vannamei.

Lactobacillus plantarum AR495 improves stress-induced irritable bowel syndrome in rats by targeting gut microbiota and Mast cell-PAR2-TRPV1 signaling pathway

Probiotics have great potential in regulating intestinal pain.In this study,the effects of Lactobacillus plantarum AR495 on the visceral sensitivity and gut microbiota of irritable bowel syndrome(IBS)rats were studied.The results showed that tryptase released after mast cell activation and degranulation plays a key role in visceral pain,and L.plantarum AR495 reduced the stimulation of colonic mast cells and the expression of protease-activated receptor 2(PAR2)and TRPV1 in dorsal root ganglia.Research further showed that supplementation with L.plantarum AR495 increased the level of short-chain fatty acids(SCFAs)and enhanced the barrier function of the colon.In addition,the microbiota analysis of the colon indicated that L.plantarum AR495 promoted the proliferation of Bifidobacterium and inhibited the proliferation of Lachnospiraceae,which alleviated the imbalance of the intestinal microbiota caused by IBS to a certain extent.In total,L.plantarum AR495 might reduce visceral sensitivity through the Mast cell-PAR2-TRPV1 signaling pathway by maintaining the homeostasis of the intestinal barrier.

Effects of Lactobacillus paracasei N1115 on gut microbial imbalance and liver function in patients with hepatitis B-related cirrhosis

BACKGROUND Hepatitis B cirrhosis(HBC)is a chronic disease characterized by irreversible diffuse liver damage and aggravated by intestinal microbial imbalance and metabolic dysfunction.Although the relationship between certain single probiotics and HBC has been explored,the impact of the complex ready-to-eat Lactobacillus paracasei N1115(LP N1115)supplement on patients with HBC has not been determined.AIM To compare the changes in the microbiota,inflammatory factor levels,and liver function before and after probiotic treatment in HBC patients.METHODS This study included 160 HBC patients diagnosed at the General Hospital of Ningxia Medical University between October 2018 and December 2020.Patients were randomly divided into an intervention group that received LP N1115 supplementation and routine treatment and a control group that received routine treatment only.Fecal samples were collected at the onset and conclusion of the 12-wk intervention period.The structure of the intestinal microbiota and the levels of serological indicators,such as liver function and inflammatory factors,were assessed.RESULTS Following LP N1115 intervention,the intestinal microbial diversity significantly increased in the intervention group(P<0.05),and the structure of the intestinal microbiota was characterized by an increase in the proportions of probiotic microbes and a reduction in harmful bacteria.Additionally,the intervention group demonstrated notable improvements in liver function indices and significantly lower levels of inflammatory factors(P<0.05).CONCLUSION LP N1115 is a promising treatment for ameliorating intestinal microbial imbalance in HBC patients by modulating the structure of the intestinal microbiota,improving liver function,and reducing inflammatory factor levels.

基于Living Lab理念的体验式教学设计与应用研究——以“高等数学”课程为例

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目的分析在神经介入医生培训中基于E-Lab手术示教系统开展脑血管造影的培训效果。方法对2022年9月至2023年3月在海军军医大学第一附属医院脑血管病中心进修的45名医生进行入组筛选,将14名符合入组条件的进修医生分为试验组和对照组,每组7人,试验组采取理论授课+基于E-Lab系统的录播案例开展培训,对照组按照传统理论授课+临床带教操作模式进行培训,比较试验组和对照组在不同培训模式下理论知识和操作技能的学习效果。结果学员的平均年龄、职称,以及培训期间造影例数、疾病类别、主动脉弓解剖类型、经桡动脉入路例数等基线指标比较,差异无统计学意义(P>0.05)。最终考核中的技能考核成绩和总成绩,2组比较差异有统计学意义(P<0.05)。其中2组的理论考核成绩差异无统计学意义(P>0.05);而技能考核中,2组导丝控制、导管控制、血管超选、操作时间、熟练程度技能得分比较,差异有统计学意义(P<0.05)。结论将E-Lab系统应用于脑血管造影术的进修学员教学培训中,对其操作技能培养有效果和优势,为神经介入的人才培养提供了新思路。

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