小麦蛋白水解物对嗜酸乳杆菌(Lactobacillus acidophilus)生长的影响研究

该研究对比了小麦蛋白水解物与不同蛋白胨对嗜酸乳杆菌生长的影响,重点从分子质量分布、氨基酸组成等方面解析了小麦蛋白水解物对嗜酸乳杆菌(Lactobacillus acidophilus)形态、活菌数、冻干存活率的影响。结果表明,小麦蛋白水解物是一种适用于嗜酸乳杆菌培养的有机氮源,同等培养条件下活菌数和冻干存活率高于其他蛋白胨,小麦蛋白水解物中分子质量为2000~5000 Da的组分是促进嗜酸乳杆菌生长的关键营养,含谷氨酰胺、脯氨酸组成的多肽可能是促进嗜酸乳杆菌缩短菌体,活菌数和冻干存活率提高的关键因素。该研究结果可为小麦蛋白水解物在嗜酸乳杆菌生产及其他发酵领域的应用提供一定的基础数据支撑。

The Latest Research Progress on Application of Lactobacillus acidophilus

Lactobacillus acidophilus belongs to the genus Lactobacillus, Gram-positive bacteria, which mainly colonize the small intestine of animals, can secrete anti-biotin substances, inhibit the growth and reproduction of some intestinal pathogenic bacteria, thereby regulating Intestinal flora, strengthen the absorption and utilization of feed nutrients, improve resistance, and promote animal growth, so it is widely used in livestock and poultry production. This article summarizes the latest research progress and application of Lactobacillus acidophilus, and looks forward to the future development direction in order to provide a theoretical basis for its future research.

牦牛肠源Lactobacillus acidophilus L3对其肠道分泌型免疫球蛋白A及免疫相关因子的影响

为了研究牦牛肠源Lactobacillus acidophilus L3（嗜酸乳杆菌）对其肠道分泌型免疫球蛋白A（SIg A）及免疫相关因子的影响,将10头健康牦牛（2～2.5岁）随机分为2组,分别为益生菌组和空白对照组,益生菌组在饲料中添加2×109CFU·kg-1L.acidophilus L3,饲喂28 d后取其小肠样品,ELISA检测试验组和对照组十二指肠、空肠、回肠中SIg A、白细胞介素-2（IL-2）、IL-4、IL-6、干扰素γ（IFN-γ）的含量。研究结果表明,L.acidophilus L3可有效增加试验组牦牛肠道SIg A的分泌量（P〈0.05）,组内比较发现SIg A在回肠含量最高,其次为空肠和十二指肠。IL-2、IL-4、IL-6在试验组小肠中的表达量较对照组显著增加（P〈0.05）,试验组小肠IFN-γ的表达量较对照组降低（P〈0.05）。证实L.acidophilus L3可提高牦牛肠道的黏膜免疫功能。

Effects of exogenous enzymes, Lactobacillus acidophilus or their combination on feed performance response and carcass characteristics of rabbits fed sugarcane bagasse

The aim of this study was to evaluate the effect of Lactobacillus acidophilus（LAC）, exogenous enzymes of ZAD（exogenous enzyme preparation） or their combination on feed conversion, and dressing of rabbits fed different treatments of sugarcane bagasse（SCB）. Five rations were allotted randomly to five groups of New-Zealand White（NZW） rabbits（n=10） with initial live body weight of（838±42.4） g and 5 weeks of age. Rabbits were fed on diets with different sources of fiber as follows,（i） a control diet composed of 100% berseem hay and 0% SCB,（ii） 50% berseem hay and 50% untreated SCB（USCB）,（iii） 50% berseem hay and 50% SCB treated with L. acidophilus（LAC）,（iv） 50% berseem hay and 50% SCB treated with ZAD（ZAD）, and（v） 50% berseem hay and 50% SCB treated with a combination of LAC＋ZAD（LZ）. Treatments of SCB with L. acidophilus, ZAD and LAC＋ZAD had the highest feed conversion ratio than both USCB and control. The dressing percentage of rabbits that fed the LAC and LZ diets was higher（P〈0.05） compared with that in the other groups. Performance index（PI） for LAC group was improved（P〈0.05） compared to that for the other groups; however, PI for USCB group was the lowest（P〈0.05）. It could be concluded that treating SCB with L. acidophilus, exogenous enzymes of ZAD or their combination improved feed conversion and performance with more positive effects with L. acidophilus than the other treatments.

牦牛肠源Lactobacillus acidophilus L3对其小肠黏膜形态结构的影响

为了探讨牦牛肠源嗜酸乳杆菌Lactobacillus acidophilus L3对牦牛小肠肠道黏膜形态结构的影响,将10头健康牦牛（2~2.5岁）随机分为2组,分别为益生菌组和空白对照组,益生菌组在饲料中添加2×109CFU·kg-1L.acidophilus L3,饲喂28 d后取其小肠组织样品,采用组织学和显微测量学方法对益生菌组和空白对照组牦牛小肠绒毛高度、隐窝深度、绒毛高度/隐窝深度比值（V/C）,以及回肠黏膜淋巴集结厚度进行测量和统计分析。研究结果显示,益生菌组牦牛十二指肠、空肠、回肠绒毛高度均较空白对照组显著增加,隐窝深度降低,V/C值增加,差异有统计学意义（P〈0.05）。通过对回肠淋巴集结的测量发现,益生菌组牦牛回肠淋巴集结的平均厚度均较空白对照组显著增加,差异有统计学意义（P〈0.05）。研究结果表明,牦牛肠源L.acidophilus L3可有效改善其肠道黏膜结构,增强肠道黏膜免疫效应部位的免疫功能。

Detection of Lactobacillus acidophilus in Fermented Material by Real-time Fluorescent Quantitative PCR

The species distinctive PCR primer of Lactobacillus acidophilus （ L. acidophilus） was designed according to 16S rRNA gene sequences of conunon Lac- tobacillus species in fermented material. Bacterial genome DNA of separated L. acidophilus in fermented sample was taken as template, and L. acidophilus in fer- mented material was conducted the quantitative determination by real-time quantitative PCR （RT-PCR）. Analysis on RT-PCR results shown that contents of L. aci- dophilus in the test sample reached 1.5 billion CFU / g. Test results shown that contents of L. acidophilus in fermented material could be detected accurately by the established RT-PCR method in the test. indicating that the established RT-PCR method could be aookued to the detection of L. acidophilus in fermented material.

Effect of Lactobacillus acidophilus as a Dietary Supplement on Nonspecific Immune Response and Disease Resistance in Juvenile Common carp, Cyprinos carpio

Effects of diet fortified with Lactobacillus acidophilus at 0.0%, 0.2%, 0.4% and 0.6% on nonspecific immune response and disease resistant common carp juvenile infected with Pseudomonas aeruginosa and Aeromonas hydrophilla were examined. Immune response parameters （the total protein, albumin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, urea, creatinine and immunoglobulin）, percentage mortality and relative protection level were determined on day 1 and days 14, 28, 42 and 56. Fish fed Lactobacilus acidophilus fortified diet significantly improved nonspecific immune parameters especially group treated with 0.4% inclusion with lower mortality rate indicating a better relative protection level against Pseudomonas aeruginosa and Aeromonas hydrophilla.

Effect of Flaxseed on Bile Tolerances of <i>Lactobacillus acidophilus, Lactobacillus bulgaricus</i>, and <i>Streptococcus thermophilus</i>

Consumption of flaxseed provides health benefits. Bile tolerance allows survival of probiotics in the intestinal tract. The objective was to determine whether or not flaxseed enhances bile tolerance of Lactobacillus acidophilus (L. acidophilus) LA-K, Lactobacillus delbruekii ssp. bulgaricus (L. bulgaricus) LB-12, and Streptococcus salivarius ssp. thermophilus (S. thermophilus) ST-M5. Control and experimental (62 g flaxseed/L) broths containing 0.3% oxgall were prepared for each culture, sterilized, cooled, inoculated, and plated for 8 h. Growth of each microorganism in both the control and experimental broths was evaluated by the slope of the regression line of its log count versus time after inoculation. Flaxseed significantly enhanced growth of L. acidophilus but not L. bulgaricus and S. thermophilus over 8 h compared to its corresponding control. Therefore, flaxseed improved the bile tolerance of L. acidophilus but not of S. thermophilus and L. bulgaricus.

Lactobacillus acidophilus alleviates pouchitis after ileal pouch-anal anastomosis in rats

AIM To assess the therapeutic potential of Lactobacillus acidophilus(LA) for the treatment of pouchitis in a rat model.METHODS Sprague Dawley rats underwent proctocolectomy and ileal pouch-anal anastomosis followed by administration of dextran sulfate sodium(DSS) to induce pouchitis. Rats with pouchitis were randomly divided into three groups: no intervention(NI), normal saline(NS, 3 m L/d normal saline for 7 d), and LA(3 m L/d LA at 1× 1010 colony-forming units for 7 d). General body condition was recorded and pouch specimens were obtained for histological examination. m RNA expression levels of interleukin(IL)-1β, IL-6, IL-10, and tumor necrosis factor-α were determined by RT-PCR. Zonula occludens protein 1(ZO-1) levels were measured by immunohistochemistry. RESULTS LA reduced weight loss associated with pouchitis(P < 0.05) and improved the symptoms of pouchitis in rats. Compared with the NI and NS groups, rats in the LAgroup showed earlier disappearance of hematochezia(6.17 ± 0.75, 6.50 ± 0.55, 3.17 ± 0.75, P < 0.05) and higher fecal scores(2.67 ± 0.48, 2.50 ± 0.51, 4.42 ± 0.50, respectively, P < 0.05). Histological scores were also lower in the LA group compared with the other two groups(7.17 ± 0.98, 8.00 ± 0.89, 4.00 ± 0.89, respectively, P < 0.05). m RNA expression levels of IL-1β, IL-6, and tumor necrosis factor-α were significantly reduced, while IL-10 m RNA levels were significantly increased in the LA group(P < 0.05, respectively). ZO-1 protein levels were also significantly increased after administration of LA(P < 0.05). CONCLUSION LA alleviates pouchitis induced by DSS after ileal pouchanal anastomosis by decreasing pro-inflammatory factors and increasing anti-inflammatory factors, and restoring ZO-1 expression in the mucosa.

Survival of <i>Lactobacillus acidophilus </i>in Fruit-Flavored Greek Yogurt Acid Whey

Greek yogurt has become much more popular within the last 15 to 20 years. The by-product of Greek yogurt manufacture is acid whey. Although acid whey has been considered a waste product, researchers are exploring various uses of this whey. Since the health benefits of consuming probiotics are widely known, one may propose adding probiotics to acid whey to form a probiotic beverage. Typically, probiotic bacteria do not thrive in acidic conditions. It would be beneficial to determine if the probiotic Lactobacillus acidophilus can survive in these acidic conditions. The objectives were to determine the growth of L. acidophilus in acid whey resulting from manufacturing Greek yogurt and to study any changes in apparent viscosity, pH, and titratable acidity over 4 weeks of refrigerated storage. Plain yogurt was manufactured, and whey was separated from plain yogurt to yield Greek yogurt and acid whey. Acid whey was batch pasteurized, cooled, sweetened, flavored with pineapple flavoring, inoculated with L. acidophilus, and stored at 4°C for 4 weeks. The log L. acidophilus counts progressively decreased from 7.84 immediately after manufacture to 2.06 at week 4. There were no significant changes in pH and titratable acidity of the pineapple-flavored probiotic acid whey over 4 weeks of storage, indicating product stability over shelf life. Viscosity changed over the storage time with minimum values at week 2 and maximum values at week 4. Although the counts declined over 4 weeks of storage, some L. acidophilus survived in the pineapple-flavored acid whey.

Antibiosis of Cefotaxime/Clindamycin and Lactobacillus acidophilus on Related Bacteria to Diabetic Foot Ulcer

Diabetic foot complications are very common and represent a serious health problem in Mexico because of their high frequency, high costs and difficulties in handling. The treatment of choice to inhibit bacteria related to diabetic foot ulcer consists mainly of the use of cefotaxime however the problem with this treatment (antibiotics) is not always effective due to the pathophysiological condition of the patient, together with the resistance bacteria develop to the drugs. OMS has suggested the use of probiotics for research directed to the development of microbial interference therapies. This project used the Lyophilized conditioned medium with probiotics, extracellulars of probiotics, because there are reports in which wound healing in mice is observed employing probiotics. The objective of this study was to evaluate the biological activity of cefotaxime, clindamycin and thelyophilized conditioned media Lactobacillus acidophilus (LCMLa) on bacterias isolated from diabetic foot ulcer, this bioassay was performed by the turbidimetric method. The macroscopic analysis of the colonies was carried out and the morphological analysis of the bacteria was carried out using the atomic force microscope;in addition, the type of Gram and oxygen requirements for its growth were determined. From the diabetic foot ulcers, three strains were isolated, of which strain 1 and 3 whose morphology corresponds to a bacillus, was susceptible to cefotaxime and to the lyophilized conditioned medium of L. acidophilus. The potential of microbial interference that exhibits L. acidophilus on bacteria related to diabetic foot ulcer is demonstrated.

Effects of Lactobacillus acidophilus on the growth performance and intestinal health of broilers challenged with Clostridium perfringens

Background: Clostridium perfringens is the main etiological agent of necrotic enteritis. Lactobacil i show beneficial effects on intestinal health in infectious disease, but the protective functions of lactobacil i in C. perfringens-infected chickens are scarcely described. This study examined the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) over a 28-day period. Using a 2 × 2 factorial arrangement of treatments, a total of 308 1-day-old male Arbor Acres broiler chicks were included to investigate the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) during a 28-day trial.Results: During infection(d 14–21), C. perfringens challenge decreased the average daily gain(P < 0.05), and increased feed conversion ratio and the mortality rate(P < 0.05). However, dietary supplementation with L. acidophilus increased the body weight of C. perfringens-infected broilers on d 21(P < 0.05), and tended to decrease the mortality(P = 0.061).C. perfringens challenge decreased the villus height(P < 0.05), the ratio of villus height to crypt depth(P < 0.05) and OCLN(occludin) m RNA expression(P < 0.05), and increased the pro-inflammatory cytokine expression in the spleen and jejunum, the intestinal populations of C. perfringens and Escherichia(P < 0.05), and the serum content of endotoxin(P < 0.05), regardless of L. acidophilus supplementation. In contrast, dietary L. acidophilus reducedthe intestinal lesion score of challenged broilers(P < 0.05), the m RNA expression of pro-inflammatory cytokines, ileal populations of Escherichia and serum endotoxin content(P < 0.05), but increased the intestinal Lactobacillus populations(P < 0.05),irrespective of C. perfringens challenge.Conclusion: Dietary addition of L. acidophilus could improve the intestinal health and reduce the mortality of broilers suffering from necrotic enteritis.

Efficacy profiles for different concentrations of Lactobacillus acidophilus in experimental colitis

AIM:To determine the efficacy profiles of different concentrations of Lactobacillus acidophilus(L.acidophilus)for treating colitis using an experimental murine model.METHODS:Colitis was established in 64 BALB/c mice by adding 5%dextran sodium sulfate(DSS)to the drinking water and allowing ad libitum access for 7 d.The mice were then randomly divided into the following control and experimental model groups(n=8 each;day 0):untreated model control;negative-treatment model control(administered gavage of 1 mL/10 g normal saline);experimental-treatment models C4-C8(administered gavage of 104,105,106,107,or 108CFU/10 g L.acidophilus,respectively);positive-treatment model control(administration of the anti-inflammatory agent prednisone acetate at 45 g/10 g).Eight mice given regular water(no DSS)and no subsequent treatments served as the normal control group.Body weight,fecal traits,and presence of fecal occult blood were assessed daily.All animals were sacrificed on post-treatment day7 to measure colonic length,perform histological scoring,and quantify the major bacteria in the proximal and distal colon.Intergroup differences were determined by one-way ANOVA and post-hoc Student-Newman-Keuls comparison.RESULTS:All treatments(L.acidophilus and prednisone acetate)protected against colitis-induced weight loss(P<0.05 vs model and normal control groups).The extent of colitis-induced colonic shortening was significantly reduced by all treatments(prednisone acetate>C4>C5>C7>C8>C6;P<0.05 vs untreated model group),and the C6 group showed colonic length similar to that of the normal control group(P>0.05).The C6 group also had the lowest disease activity index scores among the model groups.The bacterial profiles in the proximal colon were similar between all of the experimental-treatment model groups(all P>0.05).In contrast,the bacterial profile in the distal colon of the C6 group showed the distinctive features(P<0.05 vs all other experimental-treatment model groups)of Lactobacillus sp.and Bifidobacterium sp.being the most abundant bacteria and Staphylococcus aureus being the least abundant bacteria.CONCLUSION:The most therapeutically efficacious concentration of L.acidophilus(106CFU/10 g)may exert its effects by modulating the bacterial profile in the distal colon.

应用嗜酸乳杆菌(Lactobacillus acidophilus)发酵薄荷抗氧化功能研究

采用嗜酸乳杆菌发酵薄荷以保持或增强其抗氧化性。研究结果表明,经发酵后,与相应未发酵对照相比,根茎叶发酵液总抗氧化性提高至54.2%~74.9%间,清除DPPH自由基能力分别提高至67.7%~70.9%间,清除超氧阴离子自由基的能力则分别提高至29.9%~30.4%间,且与发酵前差异具有显著性(P<0.05)。进一步对发酵前后总酚、总黄酮等抗氧化物质含量分析发现,根茎叶总酚总黄酮含量在发酵后均上升,分别提升至12.0%~12.7%及15.6%~20.1%间,且茎叶与发酵前相比差异呈现显著性(P<0.05)。最终发酵产品感官品评方面,只叶部颜色与根茎差异呈现显著性,其它指标差异均不具显著性(P>0.05)。该研究对于薄荷的发酵功能性产品开发提供了新思路。

Bile tolerance of <i>Lactobacillus acidophilus</i>LA-K as influenced by various pulsed electric field conditions

Lactobacillus acidophilus has several health benefits and is used as an adjunct bacterium in the manufacture of cultured dairy foods. Bile tolerance is an important probiotic characteristic. Pulsed electric field (PEF) processing is non-thermal, hurdle technology which comprises of passing fluid foods between two electrodes and subjecting them to pulses of voltage for less than one second. Whether certain mild PEF conditions can enhance bile tolerance of Lactobacillus acidophilus is not known. The objective was to study the influence of certain PEF conditions on the, bile tolerance of Lactobacillus acidophilus LA-K. Lactobacillus acidophilus LA-K suspended in sterile peptone 0.1% w/v distilled water was treated with pulse widths of 3, 6 and 9 μs, pulse periods of 10,000;20,000 and 30,000 μs and electric field strengths of 5, 15 and 25 kV/cm. The control did not receive any pulsed electric field condition. Bile tolerance was determined hourly for 16 hours. PROC GLM of the Statistical Analysis Systems (SAS) was used for data analysis. Significant differences were determined at P p p p Lactobacillus acidophilus LA-K subjected to 5 kV/cm were signifycantly the highest while the bile tolerance when subjected to 25 kV/cm was significantly the lowest.

Acid Tolerance of <i>Lactobacillus acidophilus</i>LA-K as Influenced by Various Pulsed Electric Field Conditions

Pulsed electric field (PEF) processing involves the application of pulses of voltage for less than one second to fluid foods placed between two electrodes. Lactobacillus acidophilus is an important probiotic bacterium used for the production of fermented dairy products. Acid tolerance is an important probiotic characteristic. The influence of PEF on acid tolerance of Lactobacillus acidophilus is not known. Objective of this study was to elucidate the influence of certain PEF conditions on the acid tolerance of Lactobacillus acidophilus LA-K. Freshly thawed Lactobacillus acidophilus LA-K was suspended in sterile peptone 0.1% w/v distilled water and treated in a pilot plant PEF system. The treatments were pulse width (3, 6 and 9 μs), pulse period (10,000;20,000 and 30,000 μs) and voltage (5, 15 and 25 kV/cm). Control was run through PEF system at 60 mL/min without receiving any pulsed electric field condition. Data were analyzed using the PROC GLM of the Statistical Analysis Systems (SAS). Differences of least square means were used to determine significant differences at P Lactobacillus acidophilus LA-K lowered by increasing pulse widths and voltages but lowering pulse periods.

没食子酸对植物乳杆菌Lactobacillus plantarum的抑制和促进生长

该文以微生物生长曲线、多酚浓度变化和培养液pH值变化为指标,研究了没食子酸(GallicAcid,GA)和表没食子儿茶素没食子酸酯(Epigallocatechin Gallate,EGCG)对植物乳杆菌CICC 6253生长的影响。结果表明GA和EGCG对植物乳杆菌生长的影响具有浓度依赖性,可表现出抑制和促进的双向调节作用,且二者的作用存在构效差异性。在GA和EGCG分别在5 mg/mL和7 mg/mL以上时,两者都抑制了植物乳杆菌的生长,而当GA和EGCG分别低于4 mg/mL和6 mg/mL时二者都促进了细胞的增殖,且随质量浓度增加其促进作用增大。GA可作为碳源被植物乳杆菌迅速利用,而EGCG(4 mg/mL)因其良好的细胞亲和性表现出显著的促进作用,高效地促进了植物乳杆菌细胞的增殖,菌落数在28h内由7.42lgCFU/mL增加到12.54lgCFU/mL,与空白对照组相比增加了2个数量级以上。本研究表明,GA和EGCG均表现出对植物乳杆菌的双向调节作用,其中EGCG表现出更佳的益生特性。本文研究结果可为GA和EGCG及类似食源性多酚在益生菌生长调节和相关食品研发中提供参考。

微生态菌株Lactobacillus Acidophilus在模拟人体胃与小肠蛋白酶环境中抗性的研究

为研究微生态菌株Lactobacillusacidophilus对胃与小肠内胃酸、胃蛋白酶、胆汁盐以及胰蛋白酶的抗逆能力,以MRS为培养基,模拟人体正常胃环境pH1.60～5.00、胃蛋白酶分泌量0.592～1.482μg/mL,小肠胰蛋白酶活力14.24～72.32u/g及胆汁盐含量0.03%～0.30%时,LactobacillusacidophilusInd-1和Lactobacillusaci-dophilusLakcid在37℃温度下培养4h,用平板菌落计数法对两菌株进行活菌计数。结果表明:在pH1.60～5.00,胃蛋白酶0.592～1.482μg/mL条件下37℃培养4h,活菌数在108cfu/mL以上;在胰蛋白酶活力14.24～72.32u/g范围内、胆汁盐0.20%的条件下,活菌数可在107cfu/mL以上。说明Lactobacillusacidophilus能顺利通过胃与小肠而进入大肠,并存活下来。

Da-Cheng-Qi Decoction(大承气汤)Combined with Lactobacillus Acidophilus Improves Gastrointestinal Function of Traumatic Brain-Injured Model Mice

Objective To investigate the effects of Da-Cheng-Qi Decoction(DCQD,大承气汤)combined with Lactobacillus acidophilus(LA)on the recovery of gastrointestinal(GI)function in traumatic brain-injured(TBI)mice.Methods A total of 150 male C57BL/6 mice were randomly divided into sham-injury,normal saline(NS),DCQD(0.4 mL/day),LA(⩾1×1010 cfu/day LA),DCQD+LA(LA administration at the same dosage after 4 h of feeding DCQD),and½DCQD+LA groups(LA administration at the same dosage after 4 h of feeding½DCQD dose)by a random number table,5–8 mice in each group.The sever TBI model was constructed according to Feeney’s enhanced gravitational forces of free falling.On days 1,3,and 7 post-TBI,plasma diamine oxidase(DAO)and D-lactic acid levels were assessed by enzyme-linked immunosorbent assay(ELISA).Occludin expression in the intestinal epithelium was assessed by Western blot analysis.Transmission electron microscopy(TEM)was used to observe the morphological changes in the network structure of interstitial cells of Cajal(ICC)and change of enteric nervous system-ICC-smooth muscle cell(ENS-ICC-SMC).Immunofluorescence staining was used to detect changes in the network structure of the ICC.Results Compared with the NS group,occludin expression in the DCQD+LA group significantly increased on Day 1,3,and 7 post-TBI(P<0.05 or P<0.01).The concentration of DAO significantly decreased in the LA,DCQD,and DCQD+LA groups on Day 3 and 7,whilst the D-lactate concentrations in the LA and½DCQD+LA groups decreased on Day 1 and 3 post-injury(P<0.05 or P<0.01).The NS group experienced a great damage on the ENS-ICC-SMC network morphology and ICC network structure,and all treatment groups had some improvements,among which the DCQD+LA group presented relatively intact network morphology.Conclusions DCQD combined with LA treatment could effectively repair the intestinal mucosal barrier and improve GI motility in mice after TBI.The combination of DCQD and LA was more effective than their respective monotherapies.

Lactobacillus acidophilus XM05降血脂效果的评价及其机理的初步探讨

研究了Lactobacillus acidophilus XM05及其发酵乳对高血脂症大鼠的降血脂作用。选取48只大鼠高脂膳食饲喂建立高血脂模型,分为A、B、C、D4组,分别作如下处理:A组(高脂模型对照组)灌胃生理盐水1mL/d、B组(活菌组)灌胃2×108cfu/mL菌悬液1mL、C组(菌株灭活组)灌胃2×108cfu/mL灭活菌悬液1mL、D组(发酵乳组)灌胃浓度为2×108cfu/mL发酵乳1mL。28d后处死大鼠,测定其血脂浓度、粪便总胆固醇和总胆汁酸浓度,肝脏HMG-CoA还原酶活性。研究结果表明,摄入Lactobacillus acidophilus XM05活菌菌体及其发酵乳均能降低大鼠血液中总胆固醇、总甘油三酯、LDL胆固醇、ApoB以及肝脏HMG-CoA还原酶活性(p<0.05),同时能提高HDL胆固醇、ApoAΙ以及粪便胆汁酸的含量(p<0.05),且发酵乳的作用较活菌体显著(p<0.05)。然而,Lactobacillus acidophilus XM05灭活后对血脂无明显影响作用(p>0.05)。由此可见,Lactobacillus acidophilus XM05具有降低高血脂的作用,其降血脂机理主要通过增加总胆汁酸的排出、调节载脂蛋白水平及抑制肝脏HMG-CoA还原酶活性。