小麦蛋白水解物对嗜酸乳杆菌(Lactobacillus acidophilus)生长的影响研究

该研究对比了小麦蛋白水解物与不同蛋白胨对嗜酸乳杆菌生长的影响,重点从分子质量分布、氨基酸组成等方面解析了小麦蛋白水解物对嗜酸乳杆菌(Lactobacillus acidophilus)形态、活菌数、冻干存活率的影响。结果表明,小麦蛋白水解物是一种适用于嗜酸乳杆菌培养的有机氮源,同等培养条件下活菌数和冻干存活率高于其他蛋白胨,小麦蛋白水解物中分子质量为2000~5000 Da的组分是促进嗜酸乳杆菌生长的关键营养,含谷氨酰胺、脯氨酸组成的多肽可能是促进嗜酸乳杆菌缩短菌体,活菌数和冻干存活率提高的关键因素。该研究结果可为小麦蛋白水解物在嗜酸乳杆菌生产及其他发酵领域的应用提供一定的基础数据支撑。

嗜酸乳杆菌(Lactobacillus acidophilus)合成纳米硒特性研究

乳酸菌具有还原亚硒酸钠为纳米硒的能力。为了采用更加环境友好的方式获得纳米硒颗粒,本研究首先探索了培养基中加硒浓度、加硒时间,以及培养时长对嗜酸乳杆菌(Lactobacillus acidophilus)合成纳米硒的影响,随后对所产的纳米硒颗粒的粒径、zeta电位及抑菌活性进行研究。结果表明,当培养基中硒含量为0~600μg/mL时,硒浓度越高,转化得到的纳米硒越多;当培养基中硒浓度高于600μg/mL时,发酵液中的纳米硒含量反而减少。在嗜酸乳杆菌生长的对数中前期,即第6 h时添加亚硒酸钠,更有利于纳米硒的合成。而培养至32 h后,纳米硒几乎不再继续合成。通过扫描电镜及粒径和电位仪分析发现,嗜酸乳杆菌所产的纳米硒颗粒呈球形,且超声破碎有助于其释放;纳米硒颗粒带负电荷,电位绝对值为40~50 mV,粒径大小较多分布于170~210 nm,具有良好的稳定性。抑菌实验结果表明,嗜酸乳杆菌还原亚硒酸钠所产的纳米硒对大肠杆菌、沙门氏菌、金黄色葡萄球菌和枯草芽孢杆菌的生长均具有抑制作用。综上,嗜酸乳杆菌可以将亚硒酸钠还原合成纳米硒,且所产的纳米硒颗粒具有良好的抑菌活性。

The Latest Research Progress on Application of Lactobacillus acidophilus

Lactobacillus acidophilus belongs to the genus Lactobacillus, Gram-positive bacteria, which mainly colonize the small intestine of animals, can secrete anti-biotin substances, inhibit the growth and reproduction of some intestinal pathogenic bacteria, thereby regulating Intestinal flora, strengthen the absorption and utilization of feed nutrients, improve resistance, and promote animal growth, so it is widely used in livestock and poultry production. This article summarizes the latest research progress and application of Lactobacillus acidophilus, and looks forward to the future development direction in order to provide a theoretical basis for its future research.

Effect of Flaxseed on Bile Tolerances of <i>Lactobacillus acidophilus, Lactobacillus bulgaricus</i>, and <i>Streptococcus thermophilus</i>

Consumption of flaxseed provides health benefits. Bile tolerance allows survival of probiotics in the intestinal tract. The objective was to determine whether or not flaxseed enhances bile tolerance of Lactobacillus acidophilus (L. acidophilus) LA-K, Lactobacillus delbruekii ssp. bulgaricus (L. bulgaricus) LB-12, and Streptococcus salivarius ssp. thermophilus (S. thermophilus) ST-M5. Control and experimental (62 g flaxseed/L) broths containing 0.3% oxgall were prepared for each culture, sterilized, cooled, inoculated, and plated for 8 h. Growth of each microorganism in both the control and experimental broths was evaluated by the slope of the regression line of its log count versus time after inoculation. Flaxseed significantly enhanced growth of L. acidophilus but not L. bulgaricus and S. thermophilus over 8 h compared to its corresponding control. Therefore, flaxseed improved the bile tolerance of L. acidophilus but not of S. thermophilus and L. bulgaricus.

牦牛肠源Lactobacillus acidophilus L3对其肠道分泌型免疫球蛋白A及免疫相关因子的影响

为了研究牦牛肠源Lactobacillus acidophilus L3（嗜酸乳杆菌）对其肠道分泌型免疫球蛋白A（SIg A）及免疫相关因子的影响,将10头健康牦牛（2～2.5岁）随机分为2组,分别为益生菌组和空白对照组,益生菌组在饲料中添加2×109CFU·kg-1L.acidophilus L3,饲喂28 d后取其小肠样品,ELISA检测试验组和对照组十二指肠、空肠、回肠中SIg A、白细胞介素-2（IL-2）、IL-4、IL-6、干扰素γ（IFN-γ）的含量。研究结果表明,L.acidophilus L3可有效增加试验组牦牛肠道SIg A的分泌量（P〈0.05）,组内比较发现SIg A在回肠含量最高,其次为空肠和十二指肠。IL-2、IL-4、IL-6在试验组小肠中的表达量较对照组显著增加（P〈0.05）,试验组小肠IFN-γ的表达量较对照组降低（P〈0.05）。证实L.acidophilus L3可提高牦牛肠道的黏膜免疫功能。

牦牛肠源Lactobacillus acidophilus L3对其小肠黏膜形态结构的影响

为了探讨牦牛肠源嗜酸乳杆菌Lactobacillus acidophilus L3对牦牛小肠肠道黏膜形态结构的影响,将10头健康牦牛（2~2.5岁）随机分为2组,分别为益生菌组和空白对照组,益生菌组在饲料中添加2×109CFU·kg-1L.acidophilus L3,饲喂28 d后取其小肠组织样品,采用组织学和显微测量学方法对益生菌组和空白对照组牦牛小肠绒毛高度、隐窝深度、绒毛高度/隐窝深度比值（V/C）,以及回肠黏膜淋巴集结厚度进行测量和统计分析。研究结果显示,益生菌组牦牛十二指肠、空肠、回肠绒毛高度均较空白对照组显著增加,隐窝深度降低,V/C值增加,差异有统计学意义（P〈0.05）。通过对回肠淋巴集结的测量发现,益生菌组牦牛回肠淋巴集结的平均厚度均较空白对照组显著增加,差异有统计学意义（P〈0.05）。研究结果表明,牦牛肠源L.acidophilus L3可有效改善其肠道黏膜结构,增强肠道黏膜免疫效应部位的免疫功能。

Effects of exogenous enzymes, Lactobacillus acidophilus or their combination on feed performance response and carcass characteristics of rabbits fed sugarcane bagasse

The aim of this study was to evaluate the effect of Lactobacillus acidophilus（LAC）, exogenous enzymes of ZAD（exogenous enzyme preparation） or their combination on feed conversion, and dressing of rabbits fed different treatments of sugarcane bagasse（SCB）. Five rations were allotted randomly to five groups of New-Zealand White（NZW） rabbits（n=10） with initial live body weight of（838±42.4） g and 5 weeks of age. Rabbits were fed on diets with different sources of fiber as follows,（i） a control diet composed of 100% berseem hay and 0% SCB,（ii） 50% berseem hay and 50% untreated SCB（USCB）,（iii） 50% berseem hay and 50% SCB treated with L. acidophilus（LAC）,（iv） 50% berseem hay and 50% SCB treated with ZAD（ZAD）, and（v） 50% berseem hay and 50% SCB treated with a combination of LAC＋ZAD（LZ）. Treatments of SCB with L. acidophilus, ZAD and LAC＋ZAD had the highest feed conversion ratio than both USCB and control. The dressing percentage of rabbits that fed the LAC and LZ diets was higher（P〈0.05） compared with that in the other groups. Performance index（PI） for LAC group was improved（P〈0.05） compared to that for the other groups; however, PI for USCB group was the lowest（P〈0.05）. It could be concluded that treating SCB with L. acidophilus, exogenous enzymes of ZAD or their combination improved feed conversion and performance with more positive effects with L. acidophilus than the other treatments.

Detection of Lactobacillus acidophilus in Fermented Material by Real-time Fluorescent Quantitative PCR

The species distinctive PCR primer of Lactobacillus acidophilus （ L. acidophilus） was designed according to 16S rRNA gene sequences of conunon Lac- tobacillus species in fermented material. Bacterial genome DNA of separated L. acidophilus in fermented sample was taken as template, and L. acidophilus in fer- mented material was conducted the quantitative determination by real-time quantitative PCR （RT-PCR）. Analysis on RT-PCR results shown that contents of L. aci- dophilus in the test sample reached 1.5 billion CFU / g. Test results shown that contents of L. acidophilus in fermented material could be detected accurately by the established RT-PCR method in the test. indicating that the established RT-PCR method could be aookued to the detection of L. acidophilus in fermented material.

Effect of Lactobacillus acidophilus as a Dietary Supplement on Nonspecific Immune Response and Disease Resistance in Juvenile Common carp, Cyprinos carpio

Effects of diet fortified with Lactobacillus acidophilus at 0.0%, 0.2%, 0.4% and 0.6% on nonspecific immune response and disease resistant common carp juvenile infected with Pseudomonas aeruginosa and Aeromonas hydrophilla were examined. Immune response parameters （the total protein, albumin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, urea, creatinine and immunoglobulin）, percentage mortality and relative protection level were determined on day 1 and days 14, 28, 42 and 56. Fish fed Lactobacilus acidophilus fortified diet significantly improved nonspecific immune parameters especially group treated with 0.4% inclusion with lower mortality rate indicating a better relative protection level against Pseudomonas aeruginosa and Aeromonas hydrophilla.

Lactobacillus acidophilus alleviates pouchitis after ileal pouch-anal anastomosis in rats

AIM To assess the therapeutic potential of Lactobacillus acidophilus(LA) for the treatment of pouchitis in a rat model.METHODS Sprague Dawley rats underwent proctocolectomy and ileal pouch-anal anastomosis followed by administration of dextran sulfate sodium(DSS) to induce pouchitis. Rats with pouchitis were randomly divided into three groups: no intervention(NI), normal saline(NS, 3 m L/d normal saline for 7 d), and LA(3 m L/d LA at 1× 1010 colony-forming units for 7 d). General body condition was recorded and pouch specimens were obtained for histological examination. m RNA expression levels of interleukin(IL)-1β, IL-6, IL-10, and tumor necrosis factor-α were determined by RT-PCR. Zonula occludens protein 1(ZO-1) levels were measured by immunohistochemistry. RESULTS LA reduced weight loss associated with pouchitis(P < 0.05) and improved the symptoms of pouchitis in rats. Compared with the NI and NS groups, rats in the LAgroup showed earlier disappearance of hematochezia(6.17 ± 0.75, 6.50 ± 0.55, 3.17 ± 0.75, P < 0.05) and higher fecal scores(2.67 ± 0.48, 2.50 ± 0.51, 4.42 ± 0.50, respectively, P < 0.05). Histological scores were also lower in the LA group compared with the other two groups(7.17 ± 0.98, 8.00 ± 0.89, 4.00 ± 0.89, respectively, P < 0.05). m RNA expression levels of IL-1β, IL-6, and tumor necrosis factor-α were significantly reduced, while IL-10 m RNA levels were significantly increased in the LA group(P < 0.05, respectively). ZO-1 protein levels were also significantly increased after administration of LA(P < 0.05). CONCLUSION LA alleviates pouchitis induced by DSS after ileal pouchanal anastomosis by decreasing pro-inflammatory factors and increasing anti-inflammatory factors, and restoring ZO-1 expression in the mucosa.

Survival of <i>Lactobacillus acidophilus </i>in Fruit-Flavored Greek Yogurt Acid Whey

Greek yogurt has become much more popular within the last 15 to 20 years. The by-product of Greek yogurt manufacture is acid whey. Although acid whey has been considered a waste product, researchers are exploring various uses of this whey. Since the health benefits of consuming probiotics are widely known, one may propose adding probiotics to acid whey to form a probiotic beverage. Typically, probiotic bacteria do not thrive in acidic conditions. It would be beneficial to determine if the probiotic Lactobacillus acidophilus can survive in these acidic conditions. The objectives were to determine the growth of L. acidophilus in acid whey resulting from manufacturing Greek yogurt and to study any changes in apparent viscosity, pH, and titratable acidity over 4 weeks of refrigerated storage. Plain yogurt was manufactured, and whey was separated from plain yogurt to yield Greek yogurt and acid whey. Acid whey was batch pasteurized, cooled, sweetened, flavored with pineapple flavoring, inoculated with L. acidophilus, and stored at 4°C for 4 weeks. The log L. acidophilus counts progressively decreased from 7.84 immediately after manufacture to 2.06 at week 4. There were no significant changes in pH and titratable acidity of the pineapple-flavored probiotic acid whey over 4 weeks of storage, indicating product stability over shelf life. Viscosity changed over the storage time with minimum values at week 2 and maximum values at week 4. Although the counts declined over 4 weeks of storage, some L. acidophilus survived in the pineapple-flavored acid whey.

Antibiosis of Cefotaxime/Clindamycin and Lactobacillus acidophilus on Related Bacteria to Diabetic Foot Ulcer

Diabetic foot complications are very common and represent a serious health problem in Mexico because of their high frequency, high costs and difficulties in handling. The treatment of choice to inhibit bacteria related to diabetic foot ulcer consists mainly of the use of cefotaxime however the problem with this treatment (antibiotics) is not always effective due to the pathophysiological condition of the patient, together with the resistance bacteria develop to the drugs. OMS has suggested the use of probiotics for research directed to the development of microbial interference therapies. This project used the Lyophilized conditioned medium with probiotics, extracellulars of probiotics, because there are reports in which wound healing in mice is observed employing probiotics. The objective of this study was to evaluate the biological activity of cefotaxime, clindamycin and thelyophilized conditioned media Lactobacillus acidophilus (LCMLa) on bacterias isolated from diabetic foot ulcer, this bioassay was performed by the turbidimetric method. The macroscopic analysis of the colonies was carried out and the morphological analysis of the bacteria was carried out using the atomic force microscope;in addition, the type of Gram and oxygen requirements for its growth were determined. From the diabetic foot ulcers, three strains were isolated, of which strain 1 and 3 whose morphology corresponds to a bacillus, was susceptible to cefotaxime and to the lyophilized conditioned medium of L. acidophilus. The potential of microbial interference that exhibits L. acidophilus on bacteria related to diabetic foot ulcer is demonstrated.

Effects of Lactobacillus acidophilus on the growth performance and intestinal health of broilers challenged with Clostridium perfringens

Background: Clostridium perfringens is the main etiological agent of necrotic enteritis. Lactobacil i show beneficial effects on intestinal health in infectious disease, but the protective functions of lactobacil i in C. perfringens-infected chickens are scarcely described. This study examined the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) over a 28-day period. Using a 2 × 2 factorial arrangement of treatments, a total of 308 1-day-old male Arbor Acres broiler chicks were included to investigate the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) during a 28-day trial.Results: During infection(d 14–21), C. perfringens challenge decreased the average daily gain(P < 0.05), and increased feed conversion ratio and the mortality rate(P < 0.05). However, dietary supplementation with L. acidophilus increased the body weight of C. perfringens-infected broilers on d 21(P < 0.05), and tended to decrease the mortality(P = 0.061).C. perfringens challenge decreased the villus height(P < 0.05), the ratio of villus height to crypt depth(P < 0.05) and OCLN(occludin) m RNA expression(P < 0.05), and increased the pro-inflammatory cytokine expression in the spleen and jejunum, the intestinal populations of C. perfringens and Escherichia(P < 0.05), and the serum content of endotoxin(P < 0.05), regardless of L. acidophilus supplementation. In contrast, dietary L. acidophilus reducedthe intestinal lesion score of challenged broilers(P < 0.05), the m RNA expression of pro-inflammatory cytokines, ileal populations of Escherichia and serum endotoxin content(P < 0.05), but increased the intestinal Lactobacillus populations(P < 0.05),irrespective of C. perfringens challenge.Conclusion: Dietary addition of L. acidophilus could improve the intestinal health and reduce the mortality of broilers suffering from necrotic enteritis.

Efficacy profiles for different concentrations of Lactobacillus acidophilus in experimental colitis

AIM:To determine the efficacy profiles of different concentrations of Lactobacillus acidophilus(L.acidophilus)for treating colitis using an experimental murine model.METHODS:Colitis was established in 64 BALB/c mice by adding 5%dextran sodium sulfate(DSS)to the drinking water and allowing ad libitum access for 7 d.The mice were then randomly divided into the following control and experimental model groups(n=8 each;day 0):untreated model control;negative-treatment model control(administered gavage of 1 mL/10 g normal saline);experimental-treatment models C4-C8(administered gavage of 104,105,106,107,or 108CFU/10 g L.acidophilus,respectively);positive-treatment model control(administration of the anti-inflammatory agent prednisone acetate at 45 g/10 g).Eight mice given regular water(no DSS)and no subsequent treatments served as the normal control group.Body weight,fecal traits,and presence of fecal occult blood were assessed daily.All animals were sacrificed on post-treatment day7 to measure colonic length,perform histological scoring,and quantify the major bacteria in the proximal and distal colon.Intergroup differences were determined by one-way ANOVA and post-hoc Student-Newman-Keuls comparison.RESULTS:All treatments(L.acidophilus and prednisone acetate)protected against colitis-induced weight loss(P<0.05 vs model and normal control groups).The extent of colitis-induced colonic shortening was significantly reduced by all treatments(prednisone acetate>C4>C5>C7>C8>C6;P<0.05 vs untreated model group),and the C6 group showed colonic length similar to that of the normal control group(P>0.05).The C6 group also had the lowest disease activity index scores among the model groups.The bacterial profiles in the proximal colon were similar between all of the experimental-treatment model groups(all P>0.05).In contrast,the bacterial profile in the distal colon of the C6 group showed the distinctive features(P<0.05 vs all other experimental-treatment model groups)of Lactobacillus sp.and Bifidobacterium sp.being the most abundant bacteria and Staphylococcus aureus being the least abundant bacteria.CONCLUSION:The most therapeutically efficacious concentration of L.acidophilus(106CFU/10 g)may exert its effects by modulating the bacterial profile in the distal colon.

Effect of Lactobacillus acidophilus 1.1878 on sIgA Content in the Intestinal Mucous and Antioxidant Capacity of Broiler Chicks

[ Objective] The paper was to study the immune activities of Lactobacillus acidophilus 1. 1878 and its effect on antioxidant capacity of broiler chicks. [ Methods] Samples of intestinal mucous, serum and liver were collected from broiler chicks after oral administration of L. acidophilus 1. 1878. Secretory immunoglobulin A （slgA） content in the intestina/mucous, total antioxidant capacity （T-AOC）, level of MDA and activity of SOD and GSH-Px were determined, respec- tively. [Result] Oral administration of L. acidophilus 1. 1878 significantly improved the slgA content in jejunum and ileum mucous （P 〈0.05）. No significant differences were observed in GSH-Px activity between treatment group and control group. The concentration of T-AOC and activity of SOD in serum and liver increased significantly （P 〈 0.05 ）, while the level of MDA reduced significantly after oral administration of L. acidophilus 1.1878 （P 〈 0.05 ）. [ Conclusion ] L. acidophilus 1. 1878 can enhance the immune function of intestinal mucous and antioxidant capacity of broiler chicks.

Bile tolerance of <i>Lactobacillus acidophilus</i>LA-K as influenced by various pulsed electric field conditions

Lactobacillus acidophilus has several health benefits and is used as an adjunct bacterium in the manufacture of cultured dairy foods. Bile tolerance is an important probiotic characteristic. Pulsed electric field (PEF) processing is non-thermal, hurdle technology which comprises of passing fluid foods between two electrodes and subjecting them to pulses of voltage for less than one second. Whether certain mild PEF conditions can enhance bile tolerance of Lactobacillus acidophilus is not known. The objective was to study the influence of certain PEF conditions on the, bile tolerance of Lactobacillus acidophilus LA-K. Lactobacillus acidophilus LA-K suspended in sterile peptone 0.1% w/v distilled water was treated with pulse widths of 3, 6 and 9 μs, pulse periods of 10,000;20,000 and 30,000 μs and electric field strengths of 5, 15 and 25 kV/cm. The control did not receive any pulsed electric field condition. Bile tolerance was determined hourly for 16 hours. PROC GLM of the Statistical Analysis Systems (SAS) was used for data analysis. Significant differences were determined at P p p p Lactobacillus acidophilus LA-K subjected to 5 kV/cm were signifycantly the highest while the bile tolerance when subjected to 25 kV/cm was significantly the lowest.

Acid Tolerance of <i>Lactobacillus acidophilus</i>LA-K as Influenced by Various Pulsed Electric Field Conditions

Pulsed electric field (PEF) processing involves the application of pulses of voltage for less than one second to fluid foods placed between two electrodes. Lactobacillus acidophilus is an important probiotic bacterium used for the production of fermented dairy products. Acid tolerance is an important probiotic characteristic. The influence of PEF on acid tolerance of Lactobacillus acidophilus is not known. Objective of this study was to elucidate the influence of certain PEF conditions on the acid tolerance of Lactobacillus acidophilus LA-K. Freshly thawed Lactobacillus acidophilus LA-K was suspended in sterile peptone 0.1% w/v distilled water and treated in a pilot plant PEF system. The treatments were pulse width (3, 6 and 9 μs), pulse period (10,000;20,000 and 30,000 μs) and voltage (5, 15 and 25 kV/cm). Control was run through PEF system at 60 mL/min without receiving any pulsed electric field condition. Data were analyzed using the PROC GLM of the Statistical Analysis Systems (SAS). Differences of least square means were used to determine significant differences at P Lactobacillus acidophilus LA-K lowered by increasing pulse widths and voltages but lowering pulse periods.

应用嗜酸乳杆菌(Lactobacillus acidophilus)发酵薄荷抗氧化功能研究

采用嗜酸乳杆菌发酵薄荷以保持或增强其抗氧化性。研究结果表明,经发酵后,与相应未发酵对照相比,根茎叶发酵液总抗氧化性提高至54.2%~74.9%间,清除DPPH自由基能力分别提高至67.7%~70.9%间,清除超氧阴离子自由基的能力则分别提高至29.9%~30.4%间,且与发酵前差异具有显著性(P<0.05)。进一步对发酵前后总酚、总黄酮等抗氧化物质含量分析发现,根茎叶总酚总黄酮含量在发酵后均上升,分别提升至12.0%~12.7%及15.6%~20.1%间,且茎叶与发酵前相比差异呈现显著性(P<0.05)。最终发酵产品感官品评方面,只叶部颜色与根茎差异呈现显著性,其它指标差异均不具显著性(P>0.05)。该研究对于薄荷的发酵功能性产品开发提供了新思路。

Amelioration of dextran sodium sulphate-induced colitis in mice by treatment with Lactobacillus rhamnosus and Lactobacillus reuteri:intraspecific and interspecific patterns

Lactobacillus rhamnosus(Rh)and Lactobacillus reuteri(Re)are well-known probiotic species in inflammatory bowel disease(IBD)research.The variations between these species’efficacy against colitis,and their model of action in this regard,are intriguing and enable treatment to be individually tailored to patients.In this study,four strains each of Rh and Re were isolated from fecal samples and their draft genomes were sequenced.The anti-colitis activities of both strains involved various aspects of intestinal immune,physical,chemical,and biological barrier function.Strikingly,the tested strains exhibited considerable interspecies and intraspecies specificity in colitis amelioration.Rh strains significantly outperformed Re strains in terms of short-chain fatty acid synthesis.Nevertheless,Re strains were more effective than Rh strains in inhibiting production of inflammatory factors;promoting production of intestinal mucus,antimicrobial peptides,and tight junction proteins;and supporting the stem cell compartment.This accounts for the anti-colitis outcomes of Re strains being superior to those of Rh strains.In addition,the effective Rh and Re strains were found to express high concentrations of specific carbohydrate metabolism-and prophage-related genes,respectively.Taken together,the results of this study could assist researchers in developing effective therapies for IBD.

Antiseptic Efficacy of A Soap Made from Biosurfactants Isolated from Bacillus and Lactobacillus against Pathogenic Bacteria

The aim of our study was to use a biosurfactant produced by Bacillus and Lactobacillus isolates as an antiseptic in the formulation of local soap. A total of 60 isolates were characterized by microbiological techniques (30 Bacillus and 30 Lactobacillus) and the ability to produce biosurfactants was demonstrated by a hydrocarbon emulsification index (E24). The emulsification indexes (E24) varied from 9% to 100% for Bacillus and from 33% to 100% for Lactobacillus as well. The antagonistic assay showed that biosurfactants were able to inhibit the formation of biofilms and growth of pathogens such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Salmonella typhirium, Shigella boydii and Proteus mirabilis. The biosurfactant consortium (BioC) from Bacillus consortium and from Lactobacillus was able to inhibit biofilm formation and the pathogens growth. The BioC was stable to alkaline pH and the temperatures stability of Biosurfactant was ranging from 50°C to 90°C. The soap was made by the cold saponification process using one biosurfactant consortium formulated. This soap has a pH of 10 and showed good cleaning power and good foam stability. Similarly, the soap showed good antiseptic power and disinfection power against all pathogens tested. Handwashing is critical to preventing disease transmission. The persistence of pathogens in waste water was evaluated. The BioS produced showed good disinfection power against all pathogens tested. The valor of reduction on the hands and in the waste water was significantly more than compared to the control soaps used. This soap could be used in the prevention, fighting, and treatment of bacterial and viral infections.