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TDZ对莴苣(Lactuca sativa L.)器官发生及乙烯形成的影响 被引量:7
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作者 徐华松 陆祖军 +1 位作者 王永繁 黄学林 《广西植物》 CAS CSCD 北大核心 1999年第1期92-94,共3页
为了研究TDZ在植物器官发生中的生理作用,用不同浓度的TDZ替代MS中的BA,发现仅用1/50BA浓度(0.1mg/L)的TDZ处理,已相当于用BA处理所得芽数,可见它具有很强的细胞分裂素活性;但是TDZ又能显著促进乙烯形成,较低浓度时抑制莴苣... 为了研究TDZ在植物器官发生中的生理作用,用不同浓度的TDZ替代MS中的BA,发现仅用1/50BA浓度(0.1mg/L)的TDZ处理,已相当于用BA处理所得芽数,可见它具有很强的细胞分裂素活性;但是TDZ又能显著促进乙烯形成,较低浓度时抑制莴苣的器官发生,可能此时乙烯起着主导作用。 展开更多
关键词 TDZ 莴苣 器官发生 乙烯
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Selenium distribution and nitrate metabolism in hydroponic lettuce(Lactuca sativa L.): Effects of selenium forms and light spectra 被引量:8
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作者 BIAN Zhong-hua LEI Bo +3 位作者 CHENG Rui-feng WANG Yu LI Tao YANG Qi-chang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第1期133-144,共12页
A deficiency in selenium(Se) in the human diet is a worldwide problem. The intake of Se-rich vegetables can be a safe way to combat Se deficiency for humans. However, most leafy vegetables can accumulate a high conten... A deficiency in selenium(Se) in the human diet is a worldwide problem. The intake of Se-rich vegetables can be a safe way to combat Se deficiency for humans. However, most leafy vegetables can accumulate a high content of nitrates, which poses a potential threat to human health. Light is an important environmental factor that regulates the uptake and distribution of mineral elements and nitrogen metabolism in plants. However, the effects of Se forms and light conditions, especially light spectra, on the uptake and translocation of Se and on nitrate reduction are poorly understood. In this study, lettuce(Lactuca sativa L.) was treated with exogenous Se applied as selenate(10 mmol L^-1) and selenite(0.5 mmol L^-1) and grown under five different light spectra: fluorescent light(FL), monochromatic red LED light(R), monochromatic blue LED light(B), and mixed red and blue LED light with a red to blue light ratio at 4(R/B=4), 8(R/B=8), and 12(R/B=12), respectively. The effects of light spectra and Se forms on plant growth, photosynthetic performance, Se accumulation and nitrate reduction were investigated. The results showed that the light spectra and Se forms had significant interactions for plant growth, foliar Se accumulation and nitrate reduction. The Se concentration and nitrate content in the leaves were negatively correlated with the percentage of red light from the light sources. Compared to Se applied as selenite, exogenous Se applied as selenate was more effective in reducing nitrate via promoting nitrate reductase and glutamate synthase activities. The lowest nitrate content and highest plant biomass were observed under R/B=8 for both the selenate and selenite treatments. The significant effect of the light spectra on the root concentration factor and translocation factor of Se resulted in marked variations in the Se concentrations in the roots and leaves. Compared with FL, red and blue LED light led to significant decreases in the foliar Se concentration. The results from this study suggest that the light spectra can contribute to Se distribution and accumulation to produce vegetables with better food quality. 展开更多
关键词 SELENIUM light spectra NITRATES nitrogen metabolism enzymes LEDs lactuca sativa L.
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Two new eudesmane sesquiterpenes from Lactuca sativa var.anagustata L 被引量:4
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作者 Yi Feng Han Gui Xiu Cao Min Xia 《Chinese Chemical Letters》 SCIE CAS CSCD 2009年第10期1211-1214,共4页
Two new eudesmane sesquiterpene lactones were isolated from the stalk of Lactuca sativa vat anagustata L and their structures were elucidated by means of spectroscopic methods, including 2D NMR (1H-1H COSY, HMBC and ... Two new eudesmane sesquiterpene lactones were isolated from the stalk of Lactuca sativa vat anagustata L and their structures were elucidated by means of spectroscopic methods, including 2D NMR (1H-1H COSY, HMBC and NOESY) as 1β-O-β-D- glucopyranosyl-4α-hydroxyl-5α, 6β, 11βH-eudesma-12, 6α-olide (1) and 1β-hydroxyl-15-O-(p-methoxyphenylacetyl)-5α, 6β, 11 βH-eudesma-3-en- 12, 6a-olide (2). 展开更多
关键词 lactuca sativa var. anagustata L COMPOSITAE Eudesmane sesquiterpene
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Effects of Priming in Combination with Fungicides on Germination and Infestation of Lettuce (Lactuca sativa L.) 被引量:2
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作者 HUJin TylkowskaK 《Agricultural Sciences in China》 CAS CSCD 2005年第6期449-454,共6页
Two seed lots of lettuce (Lactuca sativa L.) cv. Krlowa Majowych were primed in polyethylene glycol (PEG) 8000 at -1.25MPa for 7 days in dark at 20C. Two fungicide treatments combined with PEG were used to control fun... Two seed lots of lettuce (Lactuca sativa L.) cv. Krlowa Majowych were primed in polyethylene glycol (PEG) 8000 at -1.25MPa for 7 days in dark at 20C. Two fungicide treatments combined with PEG were used to control fungi: 1) Funaben T(0.1%) and Apron 35 SD (0.02%) were added to -1.25 MPa PEG solution (PEG+F); 2) After seed priming and drying,Funaben T was mixed at 5 g kg-1 and Apron 35 SD at 1 g kg-1 of seeds (PEG then F dust). PEG, PEG+F and PEG then F dusttreatments reduced significantly thermodormancy and increased the seed germination at 35C in both lots. PEG then Fdust treatment had the best effect on reducing seed thermodormancy at 35C, and increased seed germination to 64.5% inlot 1 and 72.0% in lot 2 compared to 0 in untreated. PEG and PEG then F dust treatments have accelerated germinationspeed at 20 and 15C in both lots. There was a significant reduction in seed infestation in which the seeds were treatedwith PEG+F and PEG then F dust compared to untreated seeds and PEG primed seeds. PEG priming enhanced seedinfestation considerably by Alternaria alternata, Penicillium spp., Rhizopus spp. and Ulocladium spp. Mycologicalanalysis showed higher frequency of Alternaria alternata and Ulocladium spp. in untreated seeds at 20C. Priming incombination with fungicides significantly alleviated the infestation of fungi, especially Alternaria alternata infestation. 展开更多
关键词 lactuca sativa L. PRIMING GERMINATION FUNGICIDE Mycological analysis Seed infestation
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Construction of Lactuca sativa Plastid Transformation Vector and High-level Expression of gfp Gene in Escherichia coli
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作者 Siming HOU Liying ZHOU +4 位作者 Lulu BU Chunlei YANG Ting GAO Tian TIAN Zheng'an YANG 《Agricultural Biotechnology》 CAS 2016年第4期1-4,共4页
Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragment... Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragments of exogenous gene were integrated to construct lettuce plastid expression vector p Brpo AGFP,which harbored the expression cassette Prrn-gfp-aad A-Tpsb A. The results showed that the amplified flanking fragments were 1.2 and 1.1 kb in size. After sequencing,restriction digestion,ligation and transformation,lettuce plastid expression vector containing expression cassette Prrn-gfp-aad A-Tpsb A was constructed and confirmed by SDS-PAGE electrophoresis. The results of SDS-PAGE electrophoresis indicated that gfp gene was efficiently expressed under the regulation of plasmid specific promoter Prrn and terminator Tpsb A. GFP accounted for 45. 6% of total soluble proteins; inclusion bodies accounted for 47.5 % of bacterial proteins,which reached relatively high expression levels. The construction of lettuce plastid expression vector p Brpo A-GFP laid a solid foundation for establishment of subsequent lettuce plastid transformation system and genetic improvement of lettuce using various functional genes. 展开更多
关键词 lactuca sativa var. capitata L. PLASTID Expression vector gfp gene High-level expression
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Effects of Different Concentrations of Photosynthetic Bacteria Solutions on Yield and Quality of Lactuca sativa L.
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作者 穆金艳 赵兰枝 王振宇 《Agricultural Science & Technology》 CAS 2017年第9期1649-1652,共4页
The effects of different concentrations of photosynthetic bacteria solutions (CK, 0 ml photosynthetic bacteria agent+4 000 ml nutrient solution; T1, 200 ml pho- tosynthetic bacteria agent+3 800 ml nutrient solution... The effects of different concentrations of photosynthetic bacteria solutions (CK, 0 ml photosynthetic bacteria agent+4 000 ml nutrient solution; T1, 200 ml pho- tosynthetic bacteria agent+3 800 ml nutrient solution; T2, 400 ml photosynthetic bacteria agent+3 600 ml nutrient solution; T3, 600 ml photosynthetic bacteria agent+ 3 400 ml nutrient solution; T4, 800 ml photosynthetic bacteria agent+3 200 ml nutrient solution; and T5, 1 000 ml photosynthetic bacteria agent+3 000 ml nutrient solu- tion) on the leaf number, fresh mass, root vigor, vitamin C content, soluble sugar content, soluble protein content and nitrate content of hydroponic Lactuca sativa L. were investigated. The results showed that the leaf number was increased by 62.66%, the fresh mass was increased by 139.7%, the root vigor was increased by 132.04%, the vitamin C content was increased by 18.34%, the soluble protein content was increased by 16.60%, the soluble sugar content was increased by 192.37%, and the nitrate content was reduced by 69.44% in the T3 group com- pared with those in the control group. The photosynthetic bacteria solution in the T3 group reduced the content of nitrate and improved the yield and quality of hydroponic L. sativa. 展开更多
关键词 Photosynthetic bacteria lactuca sativa L. NITRATE YIELD Quality
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Environmantal Risk Phytoaccumulation of Arsenic in Spinacea Olaracea L, and Lactuca Sativa L
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作者 Mirsad Veladzic Fatima Muhamedagie Osman Perviz Ekrem Pehlic Enez Selimbegovic 《Journal of Environmental Science and Engineering(B)》 2015年第7期368-371,共4页
Pollution of the environment, particularly the soil, appears to be one of the major contemporary issues. In addition, many studies point to the involvement of ecological risk of heavy metals into the food chain throug... Pollution of the environment, particularly the soil, appears to be one of the major contemporary issues. In addition, many studies point to the involvement of ecological risk of heavy metals into the food chain through edible plants like Spinaceae oleraceae L. and Lactuca sativa L.. This paper presents the results of the "ex-situ" application of phytoacumulation on the soils of contaminated terrain using the plant species of spinach (Spinacea oleracea L.) and lettuce (Lactuca sativa L.). Sequestration of arsen from the soil by these plant species was observed through phytoaccumulation factor--PF and limit values (mg/kg). The main task and objective of this research was to establish the level of accumulation of toxic element As from the soil by plants and to calculate the PF factor of transfer. The experiment was set up in the control conditions where the soil from eight contaminated locations was placed in the experimental containers. The AAS method was used to analyze heavy metal in plant material and soil. PF factor values ranged from 0.1 to 1.0 which indicates that the spinach and lettuce plants rechargeable moderate compared to arsenic. 展开更多
关键词 PHYTOACCUMULATION Spinaceae oleraceae L. lactuca sativa L. arsenic.
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Advances in Physiological-biochemical and Genetic Mechanisms of Seed Aging in Rice(Oryza sativa L.) 被引量:4
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作者 林秋云 沈建凯 +2 位作者 谢振宇 贺治洲 尹明 《Agricultural Science & Technology》 CAS 2016年第7期1577-1584,共8页
Reasons causing or accelerating seed aging are mainly damage of mem- branes, DNA and proteins, decline of protein synthesis capacity and excessive ac- cumulation of reactive oxygen species. With the application of nat... Reasons causing or accelerating seed aging are mainly damage of mem- branes, DNA and proteins, decline of protein synthesis capacity and excessive ac- cumulation of reactive oxygen species. With the application of natural aging or artifi- cial aging methods, it was reported that quantitative trait loci (QTLs) of seed stora- bility in rice were widely distributed on the chromosomes except the 10th chromo- some. In this paper, we reviewed the progresses in the research on physiological- biochemical and genetic mechanisms of seed aging, and analyzed the existing problems and developing prospect in molecular breeding of rice with improved seed storability, in order to provide reference for the basic research and genetic improve- ment of rice seed storabUity. 展开更多
关键词 Rice (Oryza sativa L.) Seed aging Seed storability Physiological-bio- chemical: QTL
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杂交水稻不同穗萌程度种子质量差异与穗萌分级研究
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作者 贾舒涵 何璨 +4 位作者 陈敏 刘家欣 胡伟民 胡晋 关亚静 《作物学报》 CAS CSCD 北大核心 2024年第9期2310-2322,共13页
研究不同穗萌程度种子质量,对科学划分穗萌级别,优化穗萌指标计算方法至关重要。本试验以钱优0508(Qianyou 0508, QY0508)、Y两优689 (Y Liangyou 689, YLY689)两个籼型杂交水稻品种为材料,测定穗上不同穗萌程度种子的发芽指标,以及可... 研究不同穗萌程度种子质量,对科学划分穗萌级别,优化穗萌指标计算方法至关重要。本试验以钱优0508(Qianyou 0508, QY0508)、Y两优689 (Y Liangyou 689, YLY689)两个籼型杂交水稻品种为材料,测定穗上不同穗萌程度种子的发芽指标,以及可溶性糖、可溶性蛋白含量等生理指标,以此评估不同穗萌程度水稻种子的发芽质量。同时,采用自然晾干和38℃烘干2种方式干燥穗萌种子,并将种子在低温和水淹2种逆境条件下发芽,探究实际生产中不同干燥处理对已穗萌种子质量的影响,及不同穗萌程度种子萌发抗逆能力的差异。此外,由于水稻种子通常在蜡熟末期至完熟初期为最佳收获期,期间均可能遭遇不良气候条件导致种子穗萌,因此本研究对不同成熟时期的穗萌种子质量也进行了探讨。通过鉴定穗上不同萌动程度的种子质量,提出了新的水稻穗萌分级方法,即完整饱满未萌动种子为0级,胚根凸起但稃壳未开裂的种子为1级,胚根露出且可见长度不足2mm的种子为2级,胚根可见长度大于2 mm的种子为3级。与0级种子相比,1~3级穗萌种子的发芽率均显著下降,不正常幼苗率显著增加,在低温、水淹逆境下,该现象更明显。相对而言, 38℃烘干方式对种子质量的影响较自然晾干小,而授粉后35 d (35 DAP)成熟度高的种子发生穗萌后对种子质量影响较授粉后25 d (25 DAP)成熟度低的种子小。本研究经过对不同穗萌程度种子质量的研究,提出了新的穗萌分级方法,对准确评估田间水稻穗萌水平有重要价值。 展开更多
关键词 水稻 穗萌分级 穗萌率 种子质量
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两种保水剂对高寒区燕麦生长发育和产量的影响
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作者 马小龙 马祥 +3 位作者 琚泽亮 刘勇 崔文静 贾志锋 《青海大学学报》 2024年第1期32-40,共9页
为探索不同保水剂对高寒区旱作燕麦生长发育和产量的影响,对两种类型的保水剂K(颗粒型保水剂)和F(粉末型保水剂)设置4个施用量:1(11.25 kg/hm^(2))、2(22.5 kg/hm^(2))、3(33.75 kg/hm^(2))、4(45 kg/hm^(2)),以不施保水剂CK为对照,研... 为探索不同保水剂对高寒区旱作燕麦生长发育和产量的影响,对两种类型的保水剂K(颗粒型保水剂)和F(粉末型保水剂)设置4个施用量:1(11.25 kg/hm^(2))、2(22.5 kg/hm^(2))、3(33.75 kg/hm^(2))、4(45 kg/hm^(2)),以不施保水剂CK为对照,研究不同类型保水剂在不同施用量下对燕麦生长发育、产量及经济效益的影响。结果表明:随着颗粒型保水剂施用量的增加,燕麦种子产量和秸秆产量呈先增加后降低的趋势,其中K2处理最好,与CK相比,燕麦种子产量和秸秆产量分别提高32.6%和6.27%,经济效益提高31.4%;随着粉末型保水剂施用量的增加,燕麦种子产量和秸秆产量均呈增加趋势,其中F4处理最好,与CK相比,种子产量和秸秆产量分别提高34.7%和6.69%,经济效益提高27.4%。相关性和通径分析表明,燕麦种子产量与单株小穗数、单株穗粒数和单株穗粒重均呈极显著正相关(P<0.01)。综上,处理K2(22.5 kg/hm^(2))与F4(45 kg/hm^(2))均可提高高寒区燕麦产量,K2处理经济效益最高。 展开更多
关键词 燕麦 保水剂 高寒区 种子产量 秸秆产量
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不同营养液EC值对叶用莴苣生长及营养品质的影响
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作者 阳攀 姜永强 +2 位作者 文雅 张广楠 孙雪梅 《青海大学学报》 2024年第1期57-62,共6页
为明确营养液EC值对不同叶用莴苣品种生长和营养品质的影响,本研究采用单因素试验设计,设置3个不同营养液EC值(1.6、2.0、2.4 mS/cm),培养2种叶用莴苣,并测定其植株的农艺性状、光合指标及营养品质。结果表明:不同叶用莴苣品种对营养液E... 为明确营养液EC值对不同叶用莴苣品种生长和营养品质的影响,本研究采用单因素试验设计,设置3个不同营养液EC值(1.6、2.0、2.4 mS/cm),培养2种叶用莴苣,并测定其植株的农艺性状、光合指标及营养品质。结果表明:不同叶用莴苣品种对营养液EC值相应不同,当营养液EC值为1.6 mS/cm时,有利于紫霞品种的生长,表现为冠幅较大、最大叶宽较宽、光合指标较高、干物质及维生素C含量较高;当营养液EC值为2.0 mS/cm时,则有利于玛丽娜品种的生长,但营养指标较低。说明,不同叶用莴苣品种的农艺性状、光合指标及营养品质对营养液EC值反应差异较大。在叶用莴苣种植过程中,应根据叶用莴苣品种、生育期等适当调节营养液EC值,避免盲目提高营养液EC值,造成不必要的浪费。 展开更多
关键词 营养液EC值 叶用莴苣 生长 营养品质
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Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice(Oryza sativa L.) 被引量:2
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作者 Elsheikh Y M Ahmed ZHANG Yan-pei +5 位作者 YU Jian-ping Rashid M A Rehman ZHANG Zhan-ying ZHANG Hong-liang LI Jin-jie LI Zi-chao 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第4期735-743,共9页
The lower seed setting is one of the major hindrances which face grain yield in rice. One of the main reasons to cause low spikelet fertility (seed setting) is male sterility or pollen abortion. Notably, pollen abor... The lower seed setting is one of the major hindrances which face grain yield in rice. One of the main reasons to cause low spikelet fertility (seed setting) is male sterility or pollen abortion. Notably, pollen abortion has been frequently observed in advanced progenies of rice. In the present study, 149 BC2F6 individuals with significant segregation in spikelet fertility were generated from the cross between N040212 (indica) and Nipponbare (japonica) and used for primary gene mapping. Three QTLs, qSS-1, qSS-7 and qSS-9 at chromosomes 1, 7 and 9, respectively, were found to be associated with seed setting. The recombinant analysis and the physical mapping information from publicly available resources exhibited that the qSS-1, qSS-7 and qSS-9 loci were mapped to an interval of 188, 701 and 3741 kb, respectively. The seed setting responsible for QTL qSS-1 was further fine mapped to 93.5 kb by using BC2F7 population of 1 849 individuals. There are 16 possible putative genes in this 93.5 kb region. Pollen vitality tests and artificial pollination indicated that the male gamete has abnormal pollen while the female gamete was normal. These data showed that low seed setting rate relative to qSS-1 may be caused by abnormal pollen grains. These results will be useful for cloning, functional analysis of the target gene governing spikelet fertility (seed setting) and understanding the genetic bases of pollen sterility. 展开更多
关键词 rice (Oryza sativa L.) QTL mapping seed setting pollen sterility
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吡虫啉和甲基硫菌灵对油菜(Brassica napus L.)和光叶紫花苕(Vicia sativa L.)的化感作用
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作者 杨璐嘉 和绍禹 田学军 《蜜蜂杂志》 2015年第11期12-14,共3页
油菜(Brassica napus L.)和光叶紫花苕(Vicia sativa L.)为重要蜜源植物。以这2种植物种子为受体,用不同浓度(20 mg/L、40 mg/L、60 mg/L、80 mg/L、100 mg/L)的吡虫啉和(200 mg/L、400 mg/L、600 mg/L、800 mg/L、1000 mg/L)的甲基硫... 油菜(Brassica napus L.)和光叶紫花苕(Vicia sativa L.)为重要蜜源植物。以这2种植物种子为受体,用不同浓度(20 mg/L、40 mg/L、60 mg/L、80 mg/L、100 mg/L)的吡虫啉和(200 mg/L、400 mg/L、600 mg/L、800 mg/L、1000 mg/L)的甲基硫菌灵采取室内培养皿滤纸法胁迫处理,以探讨这2种农药对油菜和光叶紫花苕的化感作用。结果表明,吡虫啉和甲基硫菌灵明显降低了油菜种子活力,抑制幼苗生长;吡虫啉总体上提高了苕子种子活力,促进幼苗生长,而甲基硫菌灵则降低苕子种子活力,抑制幼苗生长。总之,吡虫啉和甲基硫菌灵对上述2种蜜源植物均有强烈的化感作用。 展开更多
关键词 吡虫啉 甲基硫菌灵 油菜(Brassica napus L.) 光叶紫花苕(Vicia sativa L.) 化感作用 种子活力
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Analysis of QTL for Seed Dormancy and Their Response to Dry Heat Treatment in Rice (Oryza sativa L.)
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作者 TANGJiu-you JIANGLing +6 位作者 ZHANGWen-wei WANGChun-ming LIUShi-jia CHENLiang-ming ZHAIHu-qu AtsushiYoshimura WANJian-min 《Agricultural Sciences in China》 CAS CSCD 2004年第9期641-648,共8页
Quantitative trait loci (QTL) controlling seed dormancy in rice were identified usingrecombinant inbred lines (RILs) population derived from the cross between a japonicavariety Kinmaze and an indica variety DV85. Seed... Quantitative trait loci (QTL) controlling seed dormancy in rice were identified usingrecombinant inbred lines (RILs) population derived from the cross between a japonicavariety Kinmaze and an indica variety DV85. Seeds of two parental cultivars and each RILwere harvested in 35d after heading. The germination percentage of these seeds at 30℃for 7 days were measured as the degree of seed dormancy. QTL analysis was performed withWindows QTL Cartographer 1.13a program by composite interval mapping. A total of four QTLfor seed dormancy were detected on chromosome 2 (two regions), 5 and 11, respectively.Phenotypic variation explained by each QTL ranged from 8.37 to 17.40%. Responses of suchloci to a dormancy-breaking treatment with dry heat were further detected. The resultsshowed that two alleles of qDOR-2-1 and qDOR-5 from DV85 as well as the allele of qDOR-11 from Kinmaze increased the seed dormancy, which seemed to be easily broken by dry heattreatment. Such loci of seed dormancy may be applied to rice genetic improvement. Theallele of qDOR-2-2 from DV85 increased the seed dormancy, which could not be broken bydry heat treatment. 展开更多
关键词 Rice(Oryza sativa L.) Seed dormancy RI population QTL analysis Dormancy- breaking by dry heat treatment
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Mapping QTLs for Salt Tolerance at Seeding Stage of Landrace Rice (Oryza sativa L.)
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作者 Jing LIN Yunhui ZHANG +4 位作者 Haiyuan CHEN Xiaomei ZHU Suobing ZHANG Yingjie WANG Xianwen FANG 《Agricultural Biotechnology》 CAS 2020年第1期12-14,25,共4页
[Objectives] The identification of salt tolerant genetic loci in rice can provide a research basis for the molecular mechanism of salt tolerance and gene resources for improving salt tolerant cultivars. [Methods] Reco... [Objectives] The identification of salt tolerant genetic loci in rice can provide a research basis for the molecular mechanism of salt tolerance and gene resources for improving salt tolerant cultivars. [Methods] Recombinant inbred lines(RILs) derived from Zhaxima, an indica landrace variety from Yunnan Province and Nanjing 46, an elite japonica variety with superior grain quality from Jiangsu Province were used. The salt tolerance at seeding stage in the RIL population was investigated as the phenotypic value. [Results] Combined with the linkage map, a total of 4 QTLs were detected: qSST-1, qSST-3, qSST-5 and qSST-11, located in rice chromosomes 1, 3, 5 and 11, respectively. All positive alleles were from the parent Nanjing 46. Three QTLs among them were not included in chromosome intervals the same as cloned rice salt tolerance genes, and thus were described as new candidate gene loci associated with seeding-stage salt tolerance. [Conclusions] This study provides important information for further exploration and utilization of new salt tolerant QTLs in rice. It is of great significance for improving the utilization of saline land in China and ensuring the stable rice production. 展开更多
关键词 Oryza sativa L. Seeding stage Salt tolerance QTL
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不同外包装方式对油麦菜贮藏品质和生理变化的影响 被引量:1
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作者 张鹏 苏娟 +5 位作者 吴迪 王赵改 马翠丽 王元方 冯仁君 李江阔 《保鲜与加工》 CAS 2023年第12期1-9,共9页
为探究不同外包装方式对油麦菜保鲜效果的影响,以油麦菜为试材,经过挑选后装入包装袋并分别置于纸箱、泡沫箱和温控箱中于4℃冷库贮藏,分析不同外包装方式对油麦菜的外观品质、营养品质、生理指标和相关酶活性的影响。结果表明:温控箱... 为探究不同外包装方式对油麦菜保鲜效果的影响,以油麦菜为试材,经过挑选后装入包装袋并分别置于纸箱、泡沫箱和温控箱中于4℃冷库贮藏,分析不同外包装方式对油麦菜的外观品质、营养品质、生理指标和相关酶活性的影响。结果表明:温控箱箱体内部温度波动范围在0.1℃以内,与纸箱组相比,温控箱的外包装处理能够有效地抑制色差值ΔE的变化,更好地维持可溶性固形物、VC和叶绿素含量的下降,有效地抑制呼吸强度和乙烯生成速率,延缓相对电导率和丙二醛含量的增加,维持过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,抑制多酚氧化酶(PPO)活性的升高。因此,温控箱包装方式下贮藏品质最好,可有效地维持油麦菜的营养品质和商品特性。 展开更多
关键词 油麦菜 温控箱 保鲜效果 生理指标 贮藏品质
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干湿交替对直播再生稻抗倒伏性和产量的影响 被引量:1
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作者 林义月 李阳 +3 位作者 张作林 汪本福 张枝盛 程建平 《湖北农业科学》 2023年第12期13-17,共5页
为了选择适合直播再生稻(Oryza sativa L.)的水分管理方式,在大田条件下,以再生稻丰两优香1号为试验材料,设置常规淹灌(CI)、轻度干湿交替(AWMD)、重度干湿交替(AWSD)3种水分处理,研究干湿交替水分处理方式对水稻茎秆倒伏指数、植株茎... 为了选择适合直播再生稻(Oryza sativa L.)的水分管理方式,在大田条件下,以再生稻丰两优香1号为试验材料,设置常规淹灌(CI)、轻度干湿交替(AWMD)、重度干湿交替(AWSD)3种水分处理,研究干湿交替水分处理方式对水稻茎秆倒伏指数、植株茎秆形态性状、头季和再生季水稻产量的影响及倒伏与产量之间的关系。结果表明,轻度干湿交替下头季稻产量最高,达8.53 t/hm2,且轻度干湿交替的头季稻每穗粒数分别比常规淹灌和重度干湿交替增加26.11%和3.73%,轻度干湿交替灌溉再生季稻产量(7.12 t/hm2)比常规淹灌和重度干湿交替增加14.47%和9.54%,轻度干湿交替的周年产量显著高于常规淹灌和重度干湿交替(P<0.05);与其他2种水分处理方式相比,轻度干湿交替降低了再生稻的倒伏指数,提高了抗折力,使再生稻茎秆重心高度下移,增强了节间粗度和茎壁厚度,从而提高了再生稻的抗倒伏能力;再生稻的产量与水稻茎秆抗倒伏性呈显著正相关(P<0.05)。综上所述,轻度干湿交替可以提高直播再生稻的抗倒伏性和产量。 展开更多
关键词 直播再生稻(Oryza sativa L.) 干湿交替 抗倒伏性 产量
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木霉对核盘菌胁迫下莴笋生理特性及生防效果的影响 被引量:1
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作者 郭书亚 艾金祥 +9 位作者 倪晓菁 王孝源 王燕燕 彭奕僖 毛纪隆 黄家妮 娄赛炜 潘蕾蕾 潘芳芳 刘鹏 《浙江师范大学学报(自然科学版)》 CAS 2023年第2期179-188,共10页
为探究哈茨木霉(Trichoderma harzianum Rifai)和绿色木霉(Trichoderma viride Pers)对菌核病的防治效果差异及其对作物的生理调节作用,在单因素实验结果的基础上,采用响应面法获得菌株最佳发酵条件.以莴笋(Lactuca sativa L.)品种‘碧... 为探究哈茨木霉(Trichoderma harzianum Rifai)和绿色木霉(Trichoderma viride Pers)对菌核病的防治效果差异及其对作物的生理调节作用,在单因素实验结果的基础上,采用响应面法获得菌株最佳发酵条件.以莴笋(Lactuca sativa L.)品种‘碧玉’为材料,探究核盘菌(Sclerotinia sclerotiorum(Lib)de Bary)处理下,哈茨木霉和绿色木霉对莴笋各项生理指标、病情指数及生防效果的影响.结果表明:2种菌株发酵液均能缓解莴笋菌核病,提高植物抗逆性,在接种9 d时,哈茨木霉组的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性均达到最高;核盘菌处理下莴笋叶片丙二醛(MDA)与脯氨酸(Pro)含量最高,施加木霉后明显下降,最大降幅分别为26.91%和31.47%;在处理15 d时,木霉组间的叶绿素荧光参数F_(v)/F_(m)(暗适应PSII最大量子产量)差异显著(P<0.05),增幅相差9.00%;哈茨木霉对菌核病的防治效果为64.17%,绿色木霉和多菌灵分别是44.73%,39.65%.综上,哈茨木霉能够抑制核盘菌生长,其对菌核病病原菌的作用效果优于绿色木霉,因此,将木霉应用于植物病害对保障作物产量具有重要意义. 展开更多
关键词 哈茨木霉 绿色木霉 核盘菌 生物防治 抗氧化酶活性 菌核病 莴笋
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不同LED组合光质对莴苣灰霉病防御酶活性的影响
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作者 刘勇鹏 王彬 +8 位作者 杨哲 任旭妍 朱新红 马肖静 孙凯乐 孙治强 朴凤植 张涛 姚秋菊 《山东农业科学》 北大核心 2023年第8期141-147,共7页
本试验在水培条件下,以LED红蓝光(红蓝光比例4∶1)为基础和对照,研究添加紫光、黄光、绿光组成的不同LED组合光质对叶用莴苣接种灰霉病菌后的病情指数以及接菌前后POD、PPO、PAL等相关防御酶活性的影响。结果表明,不同LED组合光质处理中... 本试验在水培条件下,以LED红蓝光(红蓝光比例4∶1)为基础和对照,研究添加紫光、黄光、绿光组成的不同LED组合光质对叶用莴苣接种灰霉病菌后的病情指数以及接菌前后POD、PPO、PAL等相关防御酶活性的影响。结果表明,不同LED组合光质处理中,以添加紫光的LED红蓝紫处理最能降低莴苣灰霉病病情指数;防御酶活性上,红蓝光基础上添加紫光和黄光处理的POD、PAL、PPO、GLU活性较高,添加绿光、黄光处理的CHT活性较高。整体上看,在LED红蓝光基础上添加一定比例的紫光或黄光,不仅可以有效降低莴苣灰霉病的病情指数,还可以提高莴苣体内的防御酶活性。该结果可为莴苣生产中通过光环境控制来抑制灰霉病发生提供理论参考。 展开更多
关键词 LED 组合光质 莴苣 灰霉病 防御酶
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水稻lncRNASVR及邻近SAUR基因在种子低温萌发中的表达
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作者 刘金朝 浦娜 +3 位作者 陈淳 陈伟雄 王慧 肖武名 《华南农业大学学报》 CAS CSCD 北大核心 2023年第6期896-905,共10页
【目的】长链非编码RNA(Long non-coding RNA,lncRNA)长度大于200 nt,一般不具有编码蛋白质功能。探究低温下lncRNA对邻近SAUR基因表达的影响,以期为研究水稻种子低温萌发的能力提供理论依据。【方法】lncRNA SVR由华南农业大学国家植... 【目的】长链非编码RNA(Long non-coding RNA,lncRNA)长度大于200 nt,一般不具有编码蛋白质功能。探究低温下lncRNA对邻近SAUR基因表达的影响,以期为研究水稻种子低温萌发的能力提供理论依据。【方法】lncRNA SVR由华南农业大学国家植物航天育种工程技术研究中心前期研究筛选,可以响应低温胁迫,通过生物信息学方法分析lncRNA SVR的二级结构并寻找lncRNA SVR内部的冷胁迫基序,通过qRT-PCR分析lncRNA SVR和SAUR基因的表达特性。【结果】lncRNA SVR序列中存在高度相似的冷胁迫响应基序,且在茎环连接处。表达特性分析表明,在种子萌发过程中低温胁迫会持续降低lncRNA SVR的表达,邻近的多个SAUR基因在低温胁迫下的表达量明显高于在常温下的表达量,表明lncRNA SVR的邻近SAUR基因一定程度上能够和lncRNA SVR一样响应低温胁迫。表达相关性分析表明,在低温萌发中lncRNA SVR与这些SAUR基因的表达均呈负相关关系,lncRNA SVR与OsSAUR55的表达呈显著负相关关系。进一步分析种子低温萌发中SAUR基因在lncRNA SVR敲除系中的表达情况,结果表明,在敲除系中,lncRNA SVR的下降幅度低于其在野生型中的下降幅度,OsSAUR41、OsSAUR53、OsSAUR54、OsSAUR55表达量的上升幅度均显著低于其在野生型中的上升幅度。【结论】lncRNA SVR可能负调控OsSAUR55的表达,进而响应低温胁迫。 展开更多
关键词 水稻 长链非编码RNA SAUR基因簇 低温胁迫 转录调控 种子萌发
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