Two new eudesmane sesquiterpene lactones were isolated from the stalk of Lactuca sativa vat anagustata L and their structures were elucidated by means of spectroscopic methods, including 2D NMR （1H-1H COSY, HMBC and ...Two new eudesmane sesquiterpene lactones were isolated from the stalk of Lactuca sativa vat anagustata L and their structures were elucidated by means of spectroscopic methods, including 2D NMR （1H-1H COSY, HMBC and NOESY） as 1β-O-β-D- glucopyranosyl-4α-hydroxyl-5α, 6β, 11βH-eudesma-12, 6α-olide （1） and 1β-hydroxyl-15-O-（p-methoxyphenylacetyl）-5α, 6β, 11 βH-eudesma-3-en- 12, 6a-olide （2）.展开更多
Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragment...Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragments of exogenous gene were integrated to construct lettuce plastid expression vector p Brpo AGFP,which harbored the expression cassette Prrn-gfp-aad A-Tpsb A. The results showed that the amplified flanking fragments were 1.2 and 1.1 kb in size. After sequencing,restriction digestion,ligation and transformation,lettuce plastid expression vector containing expression cassette Prrn-gfp-aad A-Tpsb A was constructed and confirmed by SDS-PAGE electrophoresis. The results of SDS-PAGE electrophoresis indicated that gfp gene was efficiently expressed under the regulation of plasmid specific promoter Prrn and terminator Tpsb A. GFP accounted for 45. 6% of total soluble proteins; inclusion bodies accounted for 47.5 % of bacterial proteins,which reached relatively high expression levels. The construction of lettuce plastid expression vector p Brpo A-GFP laid a solid foundation for establishment of subsequent lettuce plastid transformation system and genetic improvement of lettuce using various functional genes.展开更多
以生菜(Lactuca sativa L. var. ramosa Hort.)为试验材料,研究了微生物菌肥对冀西北坝上地区生菜产量和品质的影响。结果表明,施用菌肥能促进生菜生长,提高其产量,改善其品质;其中,处理M2Y2[底施木美土里微生物菌肥1 200 kg/hm2(M2)+...以生菜(Lactuca sativa L. var. ramosa Hort.)为试验材料,研究了微生物菌肥对冀西北坝上地区生菜产量和品质的影响。结果表明,施用菌肥能促进生菜生长,提高其产量,改善其品质;其中,处理M2Y2[底施木美土里微生物菌肥1 200 kg/hm2(M2)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)]生菜的株高、地上部鲜重、地上部干重、根系活力、地下部鲜重、地下部干重和产量均显著高于CK,比CK分别提高了18.27%、61.04%、88.20%、101.43%、59.34%、125.00%和64.74%,处理M2Y2生菜的可溶性糖、可溶性蛋白质、维生素C含量也明显高于CK,较CK分别增加了63.59%、42.35%和117.32%,而亚硝酸盐含量则显著低于CK,比CK降低了66.07%。施用效果最好的为处理M2Y2,其次为处理M1Y2,即底施木美土里微生物菌肥600 kg/hm2(M1)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)。展开更多
基金financed by the Science Foundation of Zhejiang Sci-Tech University(No.0613266-Y)the Talents Training Foundation of Key Laboratory of Advanced Textile Materials and Manufacturing Technology (Zhejiang Sci-Tech University),Ministry of Education(No.2006QN04)
文摘Two new eudesmane sesquiterpene lactones were isolated from the stalk of Lactuca sativa vat anagustata L and their structures were elucidated by means of spectroscopic methods, including 2D NMR （1H-1H COSY, HMBC and NOESY） as 1β-O-β-D- glucopyranosyl-4α-hydroxyl-5α, 6β, 11βH-eudesma-12, 6α-olide （1） and 1β-hydroxyl-15-O-（p-methoxyphenylacetyl）-5α, 6β, 11 βH-eudesma-3-en- 12, 6a-olide （2）.
基金Supported by Natural Science Foundation of Yunnan Province(2011FB049)National Natural Science Foundation of China(31260481,31460516)+2 种基金Fund of Yunnan Education Department(2013Y251)Fund of the Department of Life Science and Technology,Kunming University(GXKM201505)Talent Fund for PhD(YJL11015)
文摘Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragments of exogenous gene were integrated to construct lettuce plastid expression vector p Brpo AGFP,which harbored the expression cassette Prrn-gfp-aad A-Tpsb A. The results showed that the amplified flanking fragments were 1.2 and 1.1 kb in size. After sequencing,restriction digestion,ligation and transformation,lettuce plastid expression vector containing expression cassette Prrn-gfp-aad A-Tpsb A was constructed and confirmed by SDS-PAGE electrophoresis. The results of SDS-PAGE electrophoresis indicated that gfp gene was efficiently expressed under the regulation of plasmid specific promoter Prrn and terminator Tpsb A. GFP accounted for 45. 6% of total soluble proteins; inclusion bodies accounted for 47.5 % of bacterial proteins,which reached relatively high expression levels. The construction of lettuce plastid expression vector p Brpo A-GFP laid a solid foundation for establishment of subsequent lettuce plastid transformation system and genetic improvement of lettuce using various functional genes.
文摘以生菜(Lactuca sativa L. var. ramosa Hort.)为试验材料,研究了微生物菌肥对冀西北坝上地区生菜产量和品质的影响。结果表明,施用菌肥能促进生菜生长,提高其产量,改善其品质;其中,处理M2Y2[底施木美土里微生物菌肥1 200 kg/hm2(M2)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)]生菜的株高、地上部鲜重、地上部干重、根系活力、地下部鲜重、地下部干重和产量均显著高于CK,比CK分别提高了18.27%、61.04%、88.20%、101.43%、59.34%、125.00%和64.74%,处理M2Y2生菜的可溶性糖、可溶性蛋白质、维生素C含量也明显高于CK,较CK分别增加了63.59%、42.35%和117.32%,而亚硝酸盐含量则显著低于CK,比CK降低了66.07%。施用效果最好的为处理M2Y2,其次为处理M1Y2,即底施木美土里微生物菌肥600 kg/hm2(M1)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)。
