In order to study the regeneration technology of mature embryos in different rice varieties,nine japonica,nine indica and eleven hybrid rice varieties of two line or three line or superiority combinations were selecte...In order to study the regeneration technology of mature embryos in different rice varieties,nine japonica,nine indica and eleven hybrid rice varieties of two line or three line or superiority combinations were selected as explants to study the callus induction,differentiation and regeneration rates on different media.The higher callus induction （61.7-89.2%） was observed in japonica rice,when cytokinin was added at lower concentration （0.3 mg L-1 6-BA） in M8 basal medium,supplemented with 30 g L-1 sucrose,8 g L-1 agar and 2 mg L-1 2,4-D.Further,the addition of two cytokinins （2 mg L-1 6-BA,0.5 mg L-1 KT） and 1 mg L-1 NAA in the M8 basal supplemented medium resulted in 9.1-100% of the callus induction in indica rice.The percent callus induction in hybrid rice varieties was 40-86.3% when addition of 1 mg L-1 6-BA and 1 mg L-1 KT was added,and the cytokinins was required by the japonica and indica rice varieties in the M8 basal supplemented medium.It was observed that when the 0.5 mg L-1 2,4-D and 1 mg L-1 6-BA were added in japonica rice,and 0.2 mg L-1 2,4-D and 0.5 mg L-1 6-BA were added in indica and hybrid rice in the MS different media,the regeneration rates were 9.2-59.5%,3.6-87.5% and 17.2-43.2% for japonica,indica and hybrid rice,respectively.Thus,the regeneration technology with higher output is established in the mature embryos of similar rice varieties.展开更多
Four media (PESI solid, MS liquid, MS solid and ASP-C-I solid medium) were usedto induce callus from excised tissues of the kelp Laminaria japonica. Only PESI solid medium and MSsolid medium produced calli. Modified M...Four media (PESI solid, MS liquid, MS solid and ASP-C-I solid medium) were usedto induce callus from excised tissues of the kelp Laminaria japonica. Only PESI solid medium and MSsolid medium produced calli. Modified MS solid medium supplemented with mannitol (3%, W/V), yeastextract (0.1%, W/V), VB2(0. 5 mg/ml), VB12(0.5 mg/ml), kinetin (0. 108 μg/ml) and NAA(1.860μg/ml) showed much better effect on callus induction than non-modified MS solid medium. After24 days of induction 75 .5% of tissues in PESI solid medium showed callus formation. For modified MSsolid medium, after three months of induction 67. 3% of tissues dedifferentiated into calli. No calluscould be found after five months of induction in either MS liquid or ASP-C-I solid medium. When calliwere squashed and cultured in N-P enriched autoclaved seawater, MS liquld medium and ASP12-NTAliquid medium (both modified with kelp extract), differentiation of cells and regeneration of sporophyteswere only observed in ASP12-NTA medium supplemented with kelp extract. Gametophyte-like filamentsformed first, then eggs were released. It was suggested that sporophyte formation could be a process ofparthenogenesis. Sterilization techniques in tissue culture of L. japonica were also tested in this study.展开更多
In order to establish the system of high frequency plant regeneration for japonica rice mature embryos, the effects of different concentrations of CuSO4 and uniconazole on in vitro culture of mature embryos were studi...In order to establish the system of high frequency plant regeneration for japonica rice mature embryos, the effects of different concentrations of CuSO4 and uniconazole on in vitro culture of mature embryos were studied using three rice cultivars of Kongyu 131, Longjing 24, and Dongnong 425 as test materials. The results showed that callus induction and differentiation of japonica rice mature embryos were apparently improved on the medium with 10-15 μmol·L-1 CuSO4 and 0.50-1.00 mg·L-1 uniconazole. Induction and differentiation rates of different genotype rice mature embryos displayed different sensitivities to CuSO4 and uniconazole. For the callus induction frequency of three varieties, the optimal concentration of CuSO4 was 15.0 mol·L-1. When the concentration of CuSO4 was 15 μmol·L-1, the plantlet differentiation rates of Kongyu 131 and Dongnong 425 got to the highest, while the concentration of CuSO4 was 10 μmol·L-1 for Longjing 24. For the callus induction and plantlet differentiation rates of Kongyu 131 and Dongnong 425, the ideal concentration of uniconazole was 0.50 mg·L-1 and for Longjing 24 was 1.00 mg·L-1.展开更多
基金supported by the National Natural Science Foundation of China (30871459)the Key Project of Cultivate New Varieties of Genetically Modified Technology of China (2008ZX08001-004)+1 种基金the Agricultural Science and Technology Innovation Fundation of Jiangsu Province, China (CX[07]603)the Fundation of Jiangsu Academy of Agricultural Sciences, China (6510707 and 6110704)
文摘In order to study the regeneration technology of mature embryos in different rice varieties,nine japonica,nine indica and eleven hybrid rice varieties of two line or three line or superiority combinations were selected as explants to study the callus induction,differentiation and regeneration rates on different media.The higher callus induction （61.7-89.2%） was observed in japonica rice,when cytokinin was added at lower concentration （0.3 mg L-1 6-BA） in M8 basal medium,supplemented with 30 g L-1 sucrose,8 g L-1 agar and 2 mg L-1 2,4-D.Further,the addition of two cytokinins （2 mg L-1 6-BA,0.5 mg L-1 KT） and 1 mg L-1 NAA in the M8 basal supplemented medium resulted in 9.1-100% of the callus induction in indica rice.The percent callus induction in hybrid rice varieties was 40-86.3% when addition of 1 mg L-1 6-BA and 1 mg L-1 KT was added,and the cytokinins was required by the japonica and indica rice varieties in the M8 basal supplemented medium.It was observed that when the 0.5 mg L-1 2,4-D and 1 mg L-1 6-BA were added in japonica rice,and 0.2 mg L-1 2,4-D and 0.5 mg L-1 6-BA were added in indica and hybrid rice in the MS different media,the regeneration rates were 9.2-59.5%,3.6-87.5% and 17.2-43.2% for japonica,indica and hybrid rice,respectively.Thus,the regeneration technology with higher output is established in the mature embryos of similar rice varieties.
文摘Four media (PESI solid, MS liquid, MS solid and ASP-C-I solid medium) were usedto induce callus from excised tissues of the kelp Laminaria japonica. Only PESI solid medium and MSsolid medium produced calli. Modified MS solid medium supplemented with mannitol (3%, W/V), yeastextract (0.1%, W/V), VB2(0. 5 mg/ml), VB12(0.5 mg/ml), kinetin (0. 108 μg/ml) and NAA(1.860μg/ml) showed much better effect on callus induction than non-modified MS solid medium. After24 days of induction 75 .5% of tissues in PESI solid medium showed callus formation. For modified MSsolid medium, after three months of induction 67. 3% of tissues dedifferentiated into calli. No calluscould be found after five months of induction in either MS liquid or ASP-C-I solid medium. When calliwere squashed and cultured in N-P enriched autoclaved seawater, MS liquld medium and ASP12-NTAliquid medium (both modified with kelp extract), differentiation of cells and regeneration of sporophyteswere only observed in ASP12-NTA medium supplemented with kelp extract. Gametophyte-like filamentsformed first, then eggs were released. It was suggested that sporophyte formation could be a process ofparthenogenesis. Sterilization techniques in tissue culture of L. japonica were also tested in this study.
基金Supported by Program in 12th Five-year Plan Rural Areas National Science and Technology Plan(2013BAD20B04)Tackle Key in Science and Technology of Chinese Science and Technology Ministry(2011BAD35B02-01)Program in Sience and Technology of Chinese Science and Technology Ministry(2011BAD16B11)
文摘In order to establish the system of high frequency plant regeneration for japonica rice mature embryos, the effects of different concentrations of CuSO4 and uniconazole on in vitro culture of mature embryos were studied using three rice cultivars of Kongyu 131, Longjing 24, and Dongnong 425 as test materials. The results showed that callus induction and differentiation of japonica rice mature embryos were apparently improved on the medium with 10-15 μmol·L-1 CuSO4 and 0.50-1.00 mg·L-1 uniconazole. Induction and differentiation rates of different genotype rice mature embryos displayed different sensitivities to CuSO4 and uniconazole. For the callus induction frequency of three varieties, the optimal concentration of CuSO4 was 15.0 mol·L-1. When the concentration of CuSO4 was 15 μmol·L-1, the plantlet differentiation rates of Kongyu 131 and Dongnong 425 got to the highest, while the concentration of CuSO4 was 10 μmol·L-1 for Longjing 24. For the callus induction and plantlet differentiation rates of Kongyu 131 and Dongnong 425, the ideal concentration of uniconazole was 0.50 mg·L-1 and for Longjing 24 was 1.00 mg·L-1.
