AIM： To clarify the clinicopathological significance of laminin-5 y2 （LNγ2） and 133 （LNI33） chains and MMP7 expression in biliary tract cancer. METHODS： We analyzed the association between immunohistochemically...AIM： To clarify the clinicopathological significance of laminin-5 y2 （LNγ2） and 133 （LNI33） chains and MMP7 expression in biliary tract cancer. METHODS： We analyzed the association between immunohistochemically detected LNγ2, LNβ3, and MMP7 expression in biliary tract cancer and clinicopathological characteristics. Activity of MMP7 was analyzed by casein zymography. An in vitro invasion assay after treatment with MMP7-specific siRNA was performed. RESULTS： LNγ2 expression was predominantly observed in carcinoma cells at the invasive front. LNγ2 expression was seen in 57% of patients with biliary tract cancer, and was associated with depth of invasion, histologic type, and advanced stage. The expression pattern of LNβ3 was classified Into two types： invasive front dominant type （38%） and diffuse type （28%）.The invasive front dominant type was associated with histologic type and advanced stage. MMP7 positivity was correlated with LNγ2 or LNβ3 expression but not with clinicopathological characteristics. Active MMP7 detected by casein zymography was correlated with depth of invasion and advanced stage. Downregulation of MMP7 expression by siRNA resulted in a significant decrease in biliary tract cancer cell invasion in vitro.CONCLUSION： Our results suggest that LNγ2 and LNβ3, in conjunction with MMP7, play a key role in the progression of biliary tract cancer.展开更多
Platinum （Pt） implants coated with poly （3, 4-ethylenedioxythiophene）/carbon nanotube （PEDOT/CNT） composite films were implanted into the brain of rats, and the brain response was evaluated 6 weeks after the imp...Platinum （Pt） implants coated with poly （3, 4-ethylenedioxythiophene）/carbon nanotube （PEDOT/CNT） composite films were implanted into the brain of rats, and the brain response was evaluated 6 weeks after the implantation. The surface morphology of Pt implants with and without the PEDOT/CNT coating was studied using scanning electron microscopy （SEM）. After 6 weeks post-implantation, the expression of laminin （vascular endothelial marker） and neuronal nuclei （NeuN, neuronal marker） were evaluated by immnohistochemistry. It is revealed that the obvious improvements of the surface density of blood vessels and neurons aound the Pt implants with the coating, which were evidenced by laminin and NeuN staining in the zone within the distance of 150 μm to the implant interface. These results suggest the PEDOT/CNT composite films can improve the biocompatibility of the Pt electrodes while it is implanted in brain.展开更多
Objective To compare metastatic potentiality and features related to metastasis in two kinds ofhuman colorectal carcinorna (HCC) cell lines. Methods: Experimental metastatic potentiality of two kinds ofcell lines, HT2...Objective To compare metastatic potentiality and features related to metastasis in two kinds ofhuman colorectal carcinorna (HCC) cell lines. Methods: Experimental metastatic potentiality of two kinds ofcell lines, HT29 and LoVo cells were compared by using nude mice model for human colorectal carcinoma liver metastasis. The expressions of epithelial-cadherin (E-cad), laminin receptor(LNR) and type Ⅳcollagenase(Ⅳ col) in two kinds of HCC cell lines, HT29 and LoVo cells, were observed quantitatively. The intracellular CD44V6 expressed in mRNA and in protein of the HCC cell lines were detected with in situ hybridization and immunohistochemistry. Results: 100% of nude mice had liver metastasis, which demonstrated thatLoVo is a high metastatic HCC cell line. However, HT29 is a relatively low metastatic HCC cell line becauseonly 42. 9 % of nude mice transplanted HT29 cells had liver metastasis. The expressions of E--cad, LNR andIVcol of HT29 and LoVo cells in vitro were detected quantitatively. HT29 cells expressed E-cad with weakpositive on cell membrane. and LoVo cells almost expressed none. LoVo cells expressed higher level of LNRand tV col than HT29 cells did. The expression of CD44V6 in LoVo cells whether in mRNA or in protein werehigher than that of HT29 cells. Conclusion: LoVo is a high metastatic HCC cell line while HT29 is a relatively low metastatic HCC cell line. E-cadherin, LNR, IV col and CD44V6 can determine the metastatic potentialof HCC cells.展开更多
目的:利用巴斯德毕赤酵母表达系统获得重组的人层黏连蛋白LG1-3组件蛋白,为进一步研究LG1-3的结构和功能间的关系奠定基础。方法:利用RT-PCR从人胎盘组织总RNA中扩增LG1-3组件cDNA,并重组入pPICZαA,电转化入毕赤酵母GS115,利用甲醇诱...目的:利用巴斯德毕赤酵母表达系统获得重组的人层黏连蛋白LG1-3组件蛋白,为进一步研究LG1-3的结构和功能间的关系奠定基础。方法:利用RT-PCR从人胎盘组织总RNA中扩增LG1-3组件cDNA,并重组入pPICZαA,电转化入毕赤酵母GS115,利用甲醇诱导表达目的蛋白。结果和结论:获得了1 800 bp LG1-3组件cDNA,利用体外定点突变将SacⅠ酶切位点GAGCTC突变为GAGCGC,经SDS-PAGE和W estern印迹检测到相对分子质量为67.48×103的LG1-3组件蛋白的表达。展开更多
基金Supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture,Sports,Science and Technology of Japan (H.Y. and K.I.)Grants-in-Aid for Cancer Research and for the Third Term Comprehensive 10-Year Strategy for Cancer Control from the Ministry of Health,Labor and Welfare of Japan (H.Y. and K.I.)
文摘AIM： To clarify the clinicopathological significance of laminin-5 y2 （LNγ2） and 133 （LNI33） chains and MMP7 expression in biliary tract cancer. METHODS： We analyzed the association between immunohistochemically detected LNγ2, LNβ3, and MMP7 expression in biliary tract cancer and clinicopathological characteristics. Activity of MMP7 was analyzed by casein zymography. An in vitro invasion assay after treatment with MMP7-specific siRNA was performed. RESULTS： LNγ2 expression was predominantly observed in carcinoma cells at the invasive front. LNγ2 expression was seen in 57% of patients with biliary tract cancer, and was associated with depth of invasion, histologic type, and advanced stage. The expression pattern of LNβ3 was classified Into two types： invasive front dominant type （38%） and diffuse type （28%）.The invasive front dominant type was associated with histologic type and advanced stage. MMP7 positivity was correlated with LNγ2 or LNβ3 expression but not with clinicopathological characteristics. Active MMP7 detected by casein zymography was correlated with depth of invasion and advanced stage. Downregulation of MMP7 expression by siRNA resulted in a significant decrease in biliary tract cancer cell invasion in vitro.CONCLUSION： Our results suggest that LNγ2 and LNβ3, in conjunction with MMP7, play a key role in the progression of biliary tract cancer.
基金Funded by the High Tech Research and Development ("863") Program of China (2006AA02Z4E6)the National Natural Science Foundation of China (Nos. 21073136, 81271364)
文摘Platinum （Pt） implants coated with poly （3, 4-ethylenedioxythiophene）/carbon nanotube （PEDOT/CNT） composite films were implanted into the brain of rats, and the brain response was evaluated 6 weeks after the implantation. The surface morphology of Pt implants with and without the PEDOT/CNT coating was studied using scanning electron microscopy （SEM）. After 6 weeks post-implantation, the expression of laminin （vascular endothelial marker） and neuronal nuclei （NeuN, neuronal marker） were evaluated by immnohistochemistry. It is revealed that the obvious improvements of the surface density of blood vessels and neurons aound the Pt implants with the coating, which were evidenced by laminin and NeuN staining in the zone within the distance of 150 μm to the implant interface. These results suggest the PEDOT/CNT composite films can improve the biocompatibility of the Pt electrodes while it is implanted in brain.
文摘Objective To compare metastatic potentiality and features related to metastasis in two kinds ofhuman colorectal carcinorna (HCC) cell lines. Methods: Experimental metastatic potentiality of two kinds ofcell lines, HT29 and LoVo cells were compared by using nude mice model for human colorectal carcinoma liver metastasis. The expressions of epithelial-cadherin (E-cad), laminin receptor(LNR) and type Ⅳcollagenase(Ⅳ col) in two kinds of HCC cell lines, HT29 and LoVo cells, were observed quantitatively. The intracellular CD44V6 expressed in mRNA and in protein of the HCC cell lines were detected with in situ hybridization and immunohistochemistry. Results: 100% of nude mice had liver metastasis, which demonstrated thatLoVo is a high metastatic HCC cell line. However, HT29 is a relatively low metastatic HCC cell line becauseonly 42. 9 % of nude mice transplanted HT29 cells had liver metastasis. The expressions of E--cad, LNR andIVcol of HT29 and LoVo cells in vitro were detected quantitatively. HT29 cells expressed E-cad with weakpositive on cell membrane. and LoVo cells almost expressed none. LoVo cells expressed higher level of LNRand tV col than HT29 cells did. The expression of CD44V6 in LoVo cells whether in mRNA or in protein werehigher than that of HT29 cells. Conclusion: LoVo is a high metastatic HCC cell line while HT29 is a relatively low metastatic HCC cell line. E-cadherin, LNR, IV col and CD44V6 can determine the metastatic potentialof HCC cells.
文摘目的:利用巴斯德毕赤酵母表达系统获得重组的人层黏连蛋白LG1-3组件蛋白,为进一步研究LG1-3的结构和功能间的关系奠定基础。方法:利用RT-PCR从人胎盘组织总RNA中扩增LG1-3组件cDNA,并重组入pPICZαA,电转化入毕赤酵母GS115,利用甲醇诱导表达目的蛋白。结果和结论:获得了1 800 bp LG1-3组件cDNA,利用体外定点突变将SacⅠ酶切位点GAGCTC突变为GAGCGC,经SDS-PAGE和W estern印迹检测到相对分子质量为67.48×103的LG1-3组件蛋白的表达。
