Laminin 332-functionalized coating to regulate the behavior of keratinocytes and gingival mesenchymal stem cells to enhance implant soft tissue sealing

Peri-implant epithelial sealing is the first line of defense against external pathogens or stimuli;hence,an essential process to prevent peri-implantitis.Laminin 332(LN332)is the main component of the internal basal lamina and participates in peri-implant epithelial sealing by forming hemidesmosomes(HDs)with integrinα6β4.In thiswork,poly(D,L-lactide)(PDLLA)-LN332 composite coating was successfully constructed by a method similar to layer-by-layer assembly,displaying staged LN332 release for as long as 28days.The PDLLA-LN332 composite coating can activate the intracellular PI3K-Akt pathway via binding to cellular integrinα6β4,which can promote adhesion,migration and proliferation of HaCaT cells and further enhance the expression of keratinocyte HD-related molecules,including integrinα6β4,LN332 and plectin.Furthermore,the PDLLA-LN332 composite coating can promote the adhesion,spreading and proliferation of gingival mesenchymal stem cells and accelerate their epithelial differentiation.Therefore,the PDLLA-LN332 composite coating can enhance implant soft tissue sealing,warranting further in vivo study.

层黏连蛋白332在妇科肿瘤中的研究进展

层黏连蛋白332(laminin 332)是上皮组织基底膜的主要组成蛋白之一,具有促进上皮细胞黏附、迁移和细胞分化等功能。Laminin 332是由3个不同基因编码的α3链、β3链和γ2链形成的异源三聚体糖蛋白。上皮细胞内合成的laminin 332是以前体形式存在的,分泌到细胞外基质后α3链和γ2链经蛋白酶水解而成熟。生理状态下,laminin332的主要作用是维持上皮组织中上皮与基底膜的黏合。研究表明laminin 332在多种恶性肿瘤组织中过度表达,与肿瘤的发生、发展密切相关。综述laminin 332的结构、与恶性肿瘤的关系及其在妇科肿瘤中的研究进展。

A Pilot Study of Antibody Drug Therapy to Regulate Cell Adhesion in Dental Implants

Dental implant therapy is a highly effective treatment for recovering occlusion after tooth loss. An important factor in the success of dental implants is establishing strong osseointegration. If more epithelial cells migrate to the implant-bone interface than mesenchymal stem cells, effective osseointegration may fail. Therefore, controlling epithelial cell adhesion and motility would be an effective strategy to increase the success rate of osseointegration. Laminin-332 is a major component of the basement membrane and is composed of three chains (α3, β3 and γ2). It is well-known that laminin-332 regulates cellular functions such as adhesion, proliferation, apoptosis and differentiation. These biological functions depend on changes in the structural arrangement of laminin-332 by proteolytic cleavage. It is well-known that cleavage of the α3 chain between its LG domains gives laminin-332 its biological function. In this study, we focused on LG domain cleavage and developed antibodies that target the LG domain cleavage site. We attempted to change the biological function of laminin-332 to control cell adhesion for the purpose of regulating dental implant therapy.

层黏连蛋332的生物学功能与相关皮肤病的研究进展

层黏连蛋白332(Laminin-332,Ln-332)是基底膜的主要成分之一,它是真表皮连接处锚定复合物中的关键分子,在维持基底膜完整性中发挥重要作用。该蛋白作为配体可与细胞跨膜蛋白整合素α6β4、α3β1等相互作用,参与细胞的黏附、迁移。在正常皮肤中,Ln-332通过与整合素α6β4及Ⅶ型胶原连接,将半桥粒附着于真皮。当基底膜遭到破坏时,Ln-332分泌快速增加,与整合素α3β1结合,作为细胞迁移的支架,促进角质形成细胞向创面迁移,从而加速伤口愈合。编码Ln-332的相关基因突变可导致交界性大疱性表皮松解症;而对Ln-332不同亚基的自身抗体可引起抗Ln-332型黏膜类天疱疮。此外,研究发现Ln-332可促进某些上皮来源肿瘤的侵袭、转移,参与形成肿瘤微环境。

黏膜类天疱疮的研究进展

黏膜类天疱疮是指一组慢性、异质性、自身免疫性表皮下水疱病,主要累及口腔、眼睛等黏膜,其特点是具有形成瘢痕的风险。患者体内存在抗基底膜带的自身抗体,分别是针对BP180、BP230、层粘连蛋白332、整合素α6β4,和Ⅶ型胶原,其中BP180的羧基端、层粘连蛋白332被认为是主要的自身抗原。诊断主要基于临床表现、组织病理及免疫学检查。治疗上应根据受累部位,疾病严重程度及进展速度,选择合适的治疗方案,为了尽量减少不可逆的损害,多学科合作诊疗是必要的。