[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.Th...[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.The separation of active components of L.rotata was successfully achieved on Waters ACQUITY UPC column,with gradient elution carbon dioxide and methanol solvent at flow rate of 0.7 mL/min.The separation gradient elution rate was as follows:15%methanol 0-1 min,18%methanol 1-5 min,20%methanol 5-6 min,25%methanol 6-7 min,30%methanol 7-8 min,30%methanol 8-9 min,15%methanol 9-10 min.The conditions used in the separation process was as follows:column temperature 30℃;injection volume 2μL.Under the abovementioned conditions,the content of methyl glucoside was measured by 3D-full wavelength scanning.The validity of the method was tested by analyzing the precision,reproducibility,stability and accuracy.[Results]The results indicated that in terms of extraction and separation of compounds,supercritical CO2 not only has the diffusion property of gas,but also has the density and fluidity of liquid.[Conclusions]The ultra-high performance chromatography is a new technique for drug analysis.展开更多
独一味(Lamiophlomi s rotata)是唇形科濒危药用植物。根据模糊物元模型理论构建独一味产量与14个生态因子之间的模糊隶属函数,采用熵权法确定评价指标权重,利用ArcGIS平台确定青藏高原地区独一味生态适宜性空间分布,旨在探究独一味适...独一味(Lamiophlomi s rotata)是唇形科濒危药用植物。根据模糊物元模型理论构建独一味产量与14个生态因子之间的模糊隶属函数,采用熵权法确定评价指标权重,利用ArcGIS平台确定青藏高原地区独一味生态适宜性空间分布,旨在探究独一味适宜性分布状况,为濒危藏药独一味资源的动态监测及资源开发利用提供科学依据。结果表明:独一味最佳生态相似区域占研究区面积的3.13%、高度相似区域占6.34%、中度相似区域面积占8.53%、低度相似区域占6.35%、不相似区域占75.65%。独一味主要集中在青藏高原东部边缘四川、甘肃和青海高海拔地带的次生植被及逐渐退化的高山草甸区,基于GIS的模糊物元模型可评价独一味的生态适宜区域和空间分布状态,为其GAP选址和生境保护地带提供有价值的信息。展开更多
基金Collaborative Innovation in Tibetan Medicine(2019XTC013)Open Laboratory of Key Laboratory of Tibetan Medicine Basic Education(ZYYJC-19-01).
文摘[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.The separation of active components of L.rotata was successfully achieved on Waters ACQUITY UPC column,with gradient elution carbon dioxide and methanol solvent at flow rate of 0.7 mL/min.The separation gradient elution rate was as follows:15%methanol 0-1 min,18%methanol 1-5 min,20%methanol 5-6 min,25%methanol 6-7 min,30%methanol 7-8 min,30%methanol 8-9 min,15%methanol 9-10 min.The conditions used in the separation process was as follows:column temperature 30℃;injection volume 2μL.Under the abovementioned conditions,the content of methyl glucoside was measured by 3D-full wavelength scanning.The validity of the method was tested by analyzing the precision,reproducibility,stability and accuracy.[Results]The results indicated that in terms of extraction and separation of compounds,supercritical CO2 not only has the diffusion property of gas,but also has the density and fluidity of liquid.[Conclusions]The ultra-high performance chromatography is a new technique for drug analysis.
文摘独一味(Lamiophlomi s rotata)是唇形科濒危药用植物。根据模糊物元模型理论构建独一味产量与14个生态因子之间的模糊隶属函数,采用熵权法确定评价指标权重,利用ArcGIS平台确定青藏高原地区独一味生态适宜性空间分布,旨在探究独一味适宜性分布状况,为濒危藏药独一味资源的动态监测及资源开发利用提供科学依据。结果表明:独一味最佳生态相似区域占研究区面积的3.13%、高度相似区域占6.34%、中度相似区域面积占8.53%、低度相似区域占6.35%、不相似区域占75.65%。独一味主要集中在青藏高原东部边缘四川、甘肃和青海高海拔地带的次生植被及逐渐退化的高山草甸区,基于GIS的模糊物元模型可评价独一味的生态适宜区域和空间分布状态,为其GAP选址和生境保护地带提供有价值的信息。
