Objective The aim was to provide basis for molecular marker assisted selection and resistance breeding of Langya chicken. Method The genetic polymorphism of Hae III site of Mx gene 3＇ sequence in Langya chicken was ...Objective The aim was to provide basis for molecular marker assisted selection and resistance breeding of Langya chicken. Method The genetic polymorphism of Hae III site of Mx gene 3＇ sequence in Langya chicken was analyzed by PCR-RFLP. Result The results showed that Hae III site controlled by allele A and B were polymorphic in Langya chicken breeds and the allele frequencies of A and B were 0.562 and 0.438 respectively. The genotype distribution of Hae III site was significantly not in Hardy-Weinberg equilibrium （ P 〈0.01 ）. The polymorphic fragments were cloned and sequenced, and the results revealed that the fragment size was 357 bp and a deletion of 31 bp occurred in variation sequences. Conclusion It was found that Hae III-RFLP exists in Mx gene 3＇ sequence in Langya chicken breeds of Shandong Province.展开更多
[ Objective] To investigate the relationship between the egg production traits and broodiness of Shiqiza, Langya and Luqin B2 chickens. E Method] Three chicken breeds, Shiqiza chickens （a breed preserved by Shandong ...[ Objective] To investigate the relationship between the egg production traits and broodiness of Shiqiza, Langya and Luqin B2 chickens. E Method] Three chicken breeds, Shiqiza chickens （a breed preserved by Shandong Academy of Agricultural Sciences）, Langya chickens （a local breed in Shandong Province） and Luqin B2 chickens （a cultivated breed） were selected. Then their broodiness traits were observed and the egg productions were analyzed. Finally, the relationships between the egg production traits and broodiness were analyzed by SAS software. [ Result] The Shiqiza, Langya and Luqin B2. chickens were broody and their broodiness rate was 15.80%, 2.24% and 6.95%, respectively. Compared with the non-broody chickens, the egg productions of Shiqiza, Langya and Luqin B2 chickens were respectively decreased by 17.16%, 1.53% and 10.8% at the age of 43 weeks. The three breeds had significant differences in repeats of broody cycles （ P 〈 0.05）, and extremely significant differences in the broodiness duration, the the first day of broodiness, the age at the first egg, total amount of abnormal eggs and amount of double- yolked eggs （P 〈 0.01 ）. [Conclusion] The broodiness is a main factor affecting egg production traits. The degree of effects of broodiness on egg production traits is decreased successively in Shiqiza chicken, Luqin B2 chicken and Langya chicken.展开更多
基金Supported by Shandong Project of Agricultural Improved VarietyEngineering~~
文摘Objective The aim was to provide basis for molecular marker assisted selection and resistance breeding of Langya chicken. Method The genetic polymorphism of Hae III site of Mx gene 3＇ sequence in Langya chicken was analyzed by PCR-RFLP. Result The results showed that Hae III site controlled by allele A and B were polymorphic in Langya chicken breeds and the allele frequencies of A and B were 0.562 and 0.438 respectively. The genotype distribution of Hae III site was significantly not in Hardy-Weinberg equilibrium （ P 〈0.01 ）. The polymorphic fragments were cloned and sequenced, and the results revealed that the fragment size was 357 bp and a deletion of 31 bp occurred in variation sequences. Conclusion It was found that Hae III-RFLP exists in Mx gene 3＇ sequence in Langya chicken breeds of Shandong Province.
基金supported by National Industrial Science and Technology Program of China(3-46)Major Agricultural Stock Breeding Project of Shandong Province(2007LZ014)+1 种基金Earmarked Fund for Modern Agro-Industry Technology Research System(nycytx-41-z09)Innovation Fund of Shandong Academy of Agricultural Sciences
文摘[ Objective] To investigate the relationship between the egg production traits and broodiness of Shiqiza, Langya and Luqin B2 chickens. E Method] Three chicken breeds, Shiqiza chickens （a breed preserved by Shandong Academy of Agricultural Sciences）, Langya chickens （a local breed in Shandong Province） and Luqin B2 chickens （a cultivated breed） were selected. Then their broodiness traits were observed and the egg productions were analyzed. Finally, the relationships between the egg production traits and broodiness were analyzed by SAS software. [ Result] The Shiqiza, Langya and Luqin B2. chickens were broody and their broodiness rate was 15.80%, 2.24% and 6.95%, respectively. Compared with the non-broody chickens, the egg productions of Shiqiza, Langya and Luqin B2 chickens were respectively decreased by 17.16%, 1.53% and 10.8% at the age of 43 weeks. The three breeds had significant differences in repeats of broody cycles （ P 〈 0.05）, and extremely significant differences in the broodiness duration, the the first day of broodiness, the age at the first egg, total amount of abnormal eggs and amount of double- yolked eggs （P 〈 0.01 ）. [Conclusion] The broodiness is a main factor affecting egg production traits. The degree of effects of broodiness on egg production traits is decreased successively in Shiqiza chicken, Luqin B2 chicken and Langya chicken.
