Objective:To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe(OP) grade black tea with a view to develop an anti-aging skin formulation.Methods:Five concentrations(0.125,0.250,0.500...Objective:To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe(OP) grade black tea with a view to develop an anti-aging skin formulation.Methods:Five concentrations(0.125,0.250,0.500,1.000 and 2.000 mg/mL) of black tea brew(BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification.Epigallocatechin gallate(EGCG) was used as the reference agent(concentrations tested:0.012,0.025,0.050,0.100 and 0.200 mg/mL).Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid(from rooster comb)and bovine testicular hyaluronidase.Results:The results revealed that BTB had moderate[IC_(50)=(1.09±0.12) mg/mL]and dose dependent(r^2=0.94) anti-hyaluronidase activity.EGCG also exhibited dose dependent(r^2=0.93,P<0.05) anti-hyaluronidase activity which was superior[IC_(50)=(0.09±0.00) mg/mL]to BTB.Conclusions:Sri Lankan low grown orthodox OP gradc black tca has promising antihyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical.In addition,it may prove useful as a beverage in the management of allergy,some joint diseases and cnvenomation.展开更多
For tea,which is predominantly out-breeding woody perennial,genetic improvement through conventional approaches is relatively ineffective,slow and costly.As a potential tool to enhance the process,Tea Research Institu...For tea,which is predominantly out-breeding woody perennial,genetic improvement through conventional approaches is relatively ineffective,slow and costly.As a potential tool to enhance the process,Tea Research Institute of Sri Lanka(TRISL) has integrated molecular biology to supplement the conventional program and is in progress.To date,a considerable progress has been achieved in the key areas such as genetic characterization and estimation of genetic diversity,isolation and characterization of EST and genomic SSRs,construction of SSR primers,construction of genetic maps and marker assisted selection and application of genomic approaches to understand the role of secondary metabolites disease resistance in tea and in this paper,a summarized version of above studies is presented.展开更多
Objective:To evaluate inhibitory activity of methylglyoxal(MGO)mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pe...Objective:To evaluate inhibitory activity of methylglyoxal(MGO)mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pekoe grade black tea.Methods:Freeze dried black tea brew(BTB)was used as the sample in this study.Antiglycation and glycation reversing activity was studied in bovine serum albumin(BSA)-MGO model.Antioxidant properties were studied using total polyphenolic content,total flavonoid content,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power in vitro antioxidant assays.Results:The results demonstrated significant(P<0.05)and dose dependant inhibition of BSA-MGO glycation[IC_(50):(164.30±4.85)μg/mL],potentiating of its reversing activity[EC_(50):(235.39±5.37)μg/mL]and marked antioxidant properties[total polyphenolic content:(119.55±9.97)mg gallic acid equivalents/g BTB;total flavonoid content:(6.04±1.26)mg quercetin equivalents/g BTB;2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power:(3.29±0.06),(1.95±0.15)and(1.31±0.19)mmol Trolox equivalents/g BTB,respectively].No correlations were observed between antioxidant activity and BSA-MGO glycation.Conclusions:The novel properties observed for Sri Lankan orange pekoe grade black tea indicate its usefulness as a supplementary beverage in managing MGO and advanced glycation end products related diseases and ailments.展开更多
Determination of sex from the tibial dimensions has been attempted before in different populations.The formulae developed to determine sex of one population are not appropriate to be applied to another.Seven standard ...Determination of sex from the tibial dimensions has been attempted before in different populations.The formulae developed to determine sex of one population are not appropriate to be applied to another.Seven standard variables including the maximum length of tibia,proximal epiphyseal breadth,distal epiphyseal breadth,minimum circumference of shaft,anteroposterior diameter at nutrient foramen,transverse diameter at the nutrient foramen(TDNF),and circumference at the nutrient foramen obtained from 81 adult tibiae(56 male and 25 female)were analyzed to investigate the sexual dimorphism in the tibial dimensions to establish sex estimating formulae from the tibia in a contemporary Sri Lankan population.Results confirmed the existence of sexual dimorphism of the tibia.Discriminant functions obtained resulted in the classification accuracies ranging from 61.9%to 80.2%.The most dimorphic single parameter in males was the TDNF providing an accuracy of 92.9%,while in females,the minimum circumference of shaft provided an accuracy of 70.4%.The best multivariate equation utilizing two tibial dimensions resulted in an accuracy of 80.2%after cross‑validation.We envisage that sex estimating formulae established in this study for a contemporary Sri Lankan population will contribute toward the biological profiling and identification of unknown skeletal remains.展开更多
Beijing Review: What is the current situation in Sri Lanka regarding the Liberation Tigers of Tamil Eelam? Nihal Rodrigo: In 2002, the Tigers and Sri Lankan Government reached a ceasefire agreement. Though it was not ...Beijing Review: What is the current situation in Sri Lanka regarding the Liberation Tigers of Tamil Eelam? Nihal Rodrigo: In 2002, the Tigers and Sri Lankan Government reached a ceasefire agreement. Though it was not perfect, at least it temporarily stopped some of the LTTE-sponsored violence. They had many rounds of negotiations with us, but this was stopped two展开更多
'In my childhood,I read articles about Chinese master Monk Faxian’s travels in Sri Lanka and took interest in Chinese and its culture.Later,I knew Premier Zhou Enlai,who had been a friend of the Sri Lanka people ...'In my childhood,I read articles about Chinese master Monk Faxian’s travels in Sri Lanka and took interest in Chinese and its culture.Later,I knew Premier Zhou Enlai,who had been a friend of the Sri Lanka people and helped us when we were in dire need of outside support.In my view,China is like the big brother of Sri Lanka.'—Indrananda展开更多
Objective: To evaluate the physicochemical and nutritional properties of selected traditional rice varieties of Sri Lanka. Methods: Twenty three rice varieties were selected. All the varieties were studied for grain c...Objective: To evaluate the physicochemical and nutritional properties of selected traditional rice varieties of Sri Lanka. Methods: Twenty three rice varieties were selected. All the varieties were studied for grain colour, grain size and shape as physicochemical properties, while for nutritional properties, moisture, crude ash, crude protein, crude fat and total carbohydrate contents were studied. Only selected set of varieties were studied for total, soluble and insoluble dietary fiber. These properties were studied using internationally accepted standard techniques. Results: The results showed significant (P < 0.05) difference in the physicochemical and nutritional properties of the studied varieties. Out of 23 varieties tested, 20 varieties were red rices, while the rest were white rices. The grain size and shape were mostly medium bold (30%) and short bold (56%). The mean moisture, crude ash, crude protein, crude fat and total carbohydrate contents of selected varieties varied from (10.42 ± 0.25)% to (12.33 ± 0.02)%, (1.30 ± 0.14)% to (1.92 ± 0.05)%, (10.59 ± 0.12)% to (13.27 ± 0.32)%, (2.18 ± 0.10)% to (4.12 ± 0.28)% and (81.42 ± 0.25)% to (85.66 ± 0.24)%, respectively. The selected varieties had total, soluble and insoluble dietary fiber contents in the range of 4.2%–6.9%, 0.8%–2.1%and 3.1%–4.8%, respectively. The varieties Pachchaperumal, Suduru samba, Wanni Dahanala, Gonabaru and Sudu Heeneti had the highest crude protein, crude fat, crude ash, total carbohydrate and dietary fiber contents, respectively. Conclusions: Selected traditional rice varieties of Sri Lanka had physicochemical properties preferred by consumers and nutritional properties with dietary importance.展开更多
基金the National Science Foundation of Sri Lanka for funding and Sri Lanka Tea Board for organoleptic analysis. (NSF/Fellow/2011/01)
文摘Objective:To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe(OP) grade black tea with a view to develop an anti-aging skin formulation.Methods:Five concentrations(0.125,0.250,0.500,1.000 and 2.000 mg/mL) of black tea brew(BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification.Epigallocatechin gallate(EGCG) was used as the reference agent(concentrations tested:0.012,0.025,0.050,0.100 and 0.200 mg/mL).Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid(from rooster comb)and bovine testicular hyaluronidase.Results:The results revealed that BTB had moderate[IC_(50)=(1.09±0.12) mg/mL]and dose dependent(r^2=0.94) anti-hyaluronidase activity.EGCG also exhibited dose dependent(r^2=0.93,P<0.05) anti-hyaluronidase activity which was superior[IC_(50)=(0.09±0.00) mg/mL]to BTB.Conclusions:Sri Lankan low grown orthodox OP gradc black tca has promising antihyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical.In addition,it may prove useful as a beverage in the management of allergy,some joint diseases and cnvenomation.
基金the financial support from SIDA/SAREC grant for capacity building in BiotechnologyCouncil for Agricultural Research Policy(CARP)-Sri Lanka+1 种基金National Research Council,Sri Lanka(Grant No:NRC 09-066)National Science Foundation-USA and the Tea Research Institute of Sri Lanka.
文摘For tea,which is predominantly out-breeding woody perennial,genetic improvement through conventional approaches is relatively ineffective,slow and costly.As a potential tool to enhance the process,Tea Research Institute of Sri Lanka(TRISL) has integrated molecular biology to supplement the conventional program and is in progress.To date,a considerable progress has been achieved in the key areas such as genetic characterization and estimation of genetic diversity,isolation and characterization of EST and genomic SSRs,construction of SSR primers,construction of genetic maps and marker assisted selection and application of genomic approaches to understand the role of secondary metabolites disease resistance in tea and in this paper,a summarized version of above studies is presented.
文摘Objective:To evaluate inhibitory activity of methylglyoxal(MGO)mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pekoe grade black tea.Methods:Freeze dried black tea brew(BTB)was used as the sample in this study.Antiglycation and glycation reversing activity was studied in bovine serum albumin(BSA)-MGO model.Antioxidant properties were studied using total polyphenolic content,total flavonoid content,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power in vitro antioxidant assays.Results:The results demonstrated significant(P<0.05)and dose dependant inhibition of BSA-MGO glycation[IC_(50):(164.30±4.85)μg/mL],potentiating of its reversing activity[EC_(50):(235.39±5.37)μg/mL]and marked antioxidant properties[total polyphenolic content:(119.55±9.97)mg gallic acid equivalents/g BTB;total flavonoid content:(6.04±1.26)mg quercetin equivalents/g BTB;2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power:(3.29±0.06),(1.95±0.15)and(1.31±0.19)mmol Trolox equivalents/g BTB,respectively].No correlations were observed between antioxidant activity and BSA-MGO glycation.Conclusions:The novel properties observed for Sri Lankan orange pekoe grade black tea indicate its usefulness as a supplementary beverage in managing MGO and advanced glycation end products related diseases and ailments.
文摘Determination of sex from the tibial dimensions has been attempted before in different populations.The formulae developed to determine sex of one population are not appropriate to be applied to another.Seven standard variables including the maximum length of tibia,proximal epiphyseal breadth,distal epiphyseal breadth,minimum circumference of shaft,anteroposterior diameter at nutrient foramen,transverse diameter at the nutrient foramen(TDNF),and circumference at the nutrient foramen obtained from 81 adult tibiae(56 male and 25 female)were analyzed to investigate the sexual dimorphism in the tibial dimensions to establish sex estimating formulae from the tibia in a contemporary Sri Lankan population.Results confirmed the existence of sexual dimorphism of the tibia.Discriminant functions obtained resulted in the classification accuracies ranging from 61.9%to 80.2%.The most dimorphic single parameter in males was the TDNF providing an accuracy of 92.9%,while in females,the minimum circumference of shaft provided an accuracy of 70.4%.The best multivariate equation utilizing two tibial dimensions resulted in an accuracy of 80.2%after cross‑validation.We envisage that sex estimating formulae established in this study for a contemporary Sri Lankan population will contribute toward the biological profiling and identification of unknown skeletal remains.
文摘Beijing Review: What is the current situation in Sri Lanka regarding the Liberation Tigers of Tamil Eelam? Nihal Rodrigo: In 2002, the Tigers and Sri Lankan Government reached a ceasefire agreement. Though it was not perfect, at least it temporarily stopped some of the LTTE-sponsored violence. They had many rounds of negotiations with us, but this was stopped two
文摘'In my childhood,I read articles about Chinese master Monk Faxian’s travels in Sri Lanka and took interest in Chinese and its culture.Later,I knew Premier Zhou Enlai,who had been a friend of the Sri Lanka people and helped us when we were in dire need of outside support.In my view,China is like the big brother of Sri Lanka.'—Indrananda
文摘Objective: To evaluate the physicochemical and nutritional properties of selected traditional rice varieties of Sri Lanka. Methods: Twenty three rice varieties were selected. All the varieties were studied for grain colour, grain size and shape as physicochemical properties, while for nutritional properties, moisture, crude ash, crude protein, crude fat and total carbohydrate contents were studied. Only selected set of varieties were studied for total, soluble and insoluble dietary fiber. These properties were studied using internationally accepted standard techniques. Results: The results showed significant (P < 0.05) difference in the physicochemical and nutritional properties of the studied varieties. Out of 23 varieties tested, 20 varieties were red rices, while the rest were white rices. The grain size and shape were mostly medium bold (30%) and short bold (56%). The mean moisture, crude ash, crude protein, crude fat and total carbohydrate contents of selected varieties varied from (10.42 ± 0.25)% to (12.33 ± 0.02)%, (1.30 ± 0.14)% to (1.92 ± 0.05)%, (10.59 ± 0.12)% to (13.27 ± 0.32)%, (2.18 ± 0.10)% to (4.12 ± 0.28)% and (81.42 ± 0.25)% to (85.66 ± 0.24)%, respectively. The selected varieties had total, soluble and insoluble dietary fiber contents in the range of 4.2%–6.9%, 0.8%–2.1%and 3.1%–4.8%, respectively. The varieties Pachchaperumal, Suduru samba, Wanni Dahanala, Gonabaru and Sudu Heeneti had the highest crude protein, crude fat, crude ash, total carbohydrate and dietary fiber contents, respectively. Conclusions: Selected traditional rice varieties of Sri Lanka had physicochemical properties preferred by consumers and nutritional properties with dietary importance.
