The present research was designed to study the association of polymorphism of natural resistance-associated macrophage proteinl （Nrampl） with some immune function and the production performance in Large White pig. T...The present research was designed to study the association of polymorphism of natural resistance-associated macrophage proteinl （Nrampl） with some immune function and the production performance in Large White pig. The PCR-RFLP technique was applied to analyze the correlation between the polymorphisms of Nrampl gene and immune function [value of Polymorphonuclear Leukocytes （PMN） obtained by Nitroblue Tetrazolium （NBT） Reduction and effect of Cytotoxin in Monocyte] and production performance in 165 Large White pigs. The results showed that there was one Nde I restriction locus in Large White pig, and both values of PMN by NBT Reduction and effect of Cytotoxin in Monocyte in genotype BB were higher than those in genotype AB （P〈0.05）. Simultaneously, the weight of 180-day-old pigs with genotype BB was higher than that with genotype AB （P〈0.05）. The results indicated that there was a significant correlation between different genotypes of Nrampl gene and Immune function and production performance, and it can be regarded as a candidate gene of disease resistance. All these results provide valuable reference to further studies of pig disease resistance.展开更多
[Objective] The aim was to screen the foliar fertilizer and growth regulators to improve the quality of Jinhua Large and White Peach. [Method] The effects of seven foliar fertilizer and growth regulators on the major ...[Objective] The aim was to screen the foliar fertilizer and growth regulators to improve the quality of Jinhua Large and White Peach. [Method] The effects of seven foliar fertilizer and growth regulators on the major economic traits of Jinhua Large and White Peach were studied. [Result] After timely spraying of S-ABA and Fushizhuang in young fruit stage,the major economic traits of Large and White Peach including fruit weight and soluble solids content were significantly higher than that of the control,and significantly better than other treatments. In addition,Huoqing spraying treatment could obviously improve the quality of Large and White Peach. [Conclusion] Shifuzhuang,S-ABA and Huoqing spraying could significantly improve the quality of Jinhua Large and White Peach,which were suitable to be disseminated and applied in production.展开更多
The full-length cDNA of 909 bp of the osteopontin gene(OPN) was isolated from of Large White Pig and analyzed with bioinformatics methods.The results showed high proportions of Asp,Glu and Ser and verified presence of...The full-length cDNA of 909 bp of the osteopontin gene(OPN) was isolated from of Large White Pig and analyzed with bioinformatics methods.The results showed high proportions of Asp,Glu and Ser and verified presence of the special sequence Arg-Gly-Asp(RGD) in the primary structure of OPN.There were high proportions of alpha helices and strong hydrophilicity in the secondary structure. The signature sequence of OPN([KQ]-x-[TA]-x(2)-[GA]-S-S-E-E-K) was located in the first region of high homology.Two phylogenetic trees were constructed,based on the entire OPN protein sequence and the conserved signature sequence,and showed that the relationship between pig and cow was the closest, but farthest between pig and chicken.OPN mRNA was expressed in many tissues of the pig:higher in the stomach,kidney,lung,small intestine and ovary,and lower in the heart,spleen and large intestine.The OPN protein size differed in different tissues:70 kDa in liver and muscle,70 and 45 kDa in stomach,small intestines and kidney,70,45 and 24 kDa in lung,heart and uterus.展开更多
In present study, the developmental patterns of somatostatin (SS) gene expression in gastric tissue were compared between Erhualian and Large White pigs. A semi-quantitative RT-PCR was applied to determine the levels ...In present study, the developmental patterns of somatostatin (SS) gene expression in gastric tissue were compared between Erhualian and Large White pigs. A semi-quantitative RT-PCR was applied to determine the levels of SS mRNA. The results indicated that: (1) The gastric SS mRNA expression was high at birth, followed by a significant decrease (P<0. 05) at 3 days of age in both breeds of pigs; (2) From 3 to 30 days of age, the expression of SS mRNA in gastric tissue exhibited remarkable up-regulation in both breeds, after which a strain difference in the developmental pattern was observed. In Erhualian pigs, SS mRNA expression reached a peak at 90 days of age, declined thereafter towards 180 days of age. In Large White pigs, however, the expression of SS mRNA remained constant from 30 days of age onwards; (3) In general, Erhualian pigs expressed higher levels of SS mRNA in gastric tissue compared with Large White pigs at the same age. The strain difference was significant from birth to 90 days of age, but vanished at 120 and 180 days of age. The results suggest that the gastric expression of SS in the pig is regulated following an instinct timetable in a strain-specific manner, its relationship with the development of gastric function as well as its interactions with environmental factors are to be elucidated.展开更多
The security of civil engineering is an important task due to the economic, social and environmental significance. Compared with conventional sensors, the optical fiber sensors have their unique characteristics.Being ...The security of civil engineering is an important task due to the economic, social and environmental significance. Compared with conventional sensors, the optical fiber sensors have their unique characteristics.Being durable, stable and insensitive to external perturbations,they are particular interesting for the long-term monitoring of civil structures.Focus is on absolute measurement optical fiber sensors, which are emerging from the monitoring large structural, including SOFO system, F-P optical fiber sensors, and fiber Bragg grating sensors. The principle, characteristic and application of these three kinds of optical fiber sensors are described together with their future prospects.展开更多
为研究胎次和配种季节对长大二元杂交母猪繁殖性能的影响,本试验选取了11333窝长大二元杂交母猪的产仔数据,包括总产仔数(Total number born,TNB)、产活仔数(Number born alive,NBA)、产弱仔数(Number of weak births,NWB)、仔猪出生窝...为研究胎次和配种季节对长大二元杂交母猪繁殖性能的影响,本试验选取了11333窝长大二元杂交母猪的产仔数据,包括总产仔数(Total number born,TNB)、产活仔数(Number born alive,NBA)、产弱仔数(Number of weak births,NWB)、仔猪出生窝重(Litter birth weight,LBW)、仔猪断奶数(Number of wearning,NW)、妊娠时间(Time to pregnancy,TTP)、产死胎数(Number of stillborn,NSB)等7个指标,分析了配种季节和胎次共同作用对长大二元杂交猪繁殖性能的影响。结果表明,4、5、6胎次母猪的总产仔数显著高于其他胎次(P<0.05),其中6胎次母猪的总产仔数最高;且4胎次母猪产活仔数显著高于其他胎次(P<0.05);2胎次母猪产弱仔数显著低于其他胎次(P<0.05);4胎次母猪所产仔猪的出生窝重显著高于其他胎次(P<0.05),第2胎母猪妊娠时间显著低于其他胎次(P<0.05),初产母猪妊娠时间显著高于其他胎次(P<0.05);发现母猪产死胎数随胎次增加而呈上升趋势,在7胎次及以上达到最高(P<0.05)。母猪的胎次与总产仔数、产活仔数、仔猪出生窝重、仔猪断奶数、产死胎数呈极显著正相关关系(P<0.01);胎次与妊娠时间呈极显著负相关关系(P<0.01)。第4胎次母猪在秋冬季配种产仔性能最好,第2胎次母猪在夏季配种产仔性能最好,5胎次母猪在春季配种产仔性能最好,第6胎次母猪在秋季配种产仔性能最好;初产母猪在秋季配种产仔性能最好,7胎次及以上母猪在春季配种产仔性能最好。胎次和季节对母猪的产仔性能及仔猪生产性能的影响研究,可以为养猪企业合理利用母猪年限提供数据参考。展开更多
文摘The present research was designed to study the association of polymorphism of natural resistance-associated macrophage proteinl （Nrampl） with some immune function and the production performance in Large White pig. The PCR-RFLP technique was applied to analyze the correlation between the polymorphisms of Nrampl gene and immune function [value of Polymorphonuclear Leukocytes （PMN） obtained by Nitroblue Tetrazolium （NBT） Reduction and effect of Cytotoxin in Monocyte] and production performance in 165 Large White pigs. The results showed that there was one Nde I restriction locus in Large White pig, and both values of PMN by NBT Reduction and effect of Cytotoxin in Monocyte in genotype BB were higher than those in genotype AB （P〈0.05）. Simultaneously, the weight of 180-day-old pigs with genotype BB was higher than that with genotype AB （P〈0.05）. The results indicated that there was a significant correlation between different genotypes of Nrampl gene and Immune function and production performance, and it can be regarded as a candidate gene of disease resistance. All these results provide valuable reference to further studies of pig disease resistance.
基金Supported by Science and Technology Projects in Zhejiang Province(2009C32036)~~
文摘[Objective] The aim was to screen the foliar fertilizer and growth regulators to improve the quality of Jinhua Large and White Peach. [Method] The effects of seven foliar fertilizer and growth regulators on the major economic traits of Jinhua Large and White Peach were studied. [Result] After timely spraying of S-ABA and Fushizhuang in young fruit stage,the major economic traits of Large and White Peach including fruit weight and soluble solids content were significantly higher than that of the control,and significantly better than other treatments. In addition,Huoqing spraying treatment could obviously improve the quality of Large and White Peach. [Conclusion] Shifuzhuang,S-ABA and Huoqing spraying could significantly improve the quality of Jinhua Large and White Peach,which were suitable to be disseminated and applied in production.
基金supported by Scientific and Technological Project in Heilongjiang Province (GB05B 106)Postdoctoral Fund of Heilongjiang Province(LRB07-392)
文摘The full-length cDNA of 909 bp of the osteopontin gene(OPN) was isolated from of Large White Pig and analyzed with bioinformatics methods.The results showed high proportions of Asp,Glu and Ser and verified presence of the special sequence Arg-Gly-Asp(RGD) in the primary structure of OPN.There were high proportions of alpha helices and strong hydrophilicity in the secondary structure. The signature sequence of OPN([KQ]-x-[TA]-x(2)-[GA]-S-S-E-E-K) was located in the first region of high homology.Two phylogenetic trees were constructed,based on the entire OPN protein sequence and the conserved signature sequence,and showed that the relationship between pig and cow was the closest, but farthest between pig and chicken.OPN mRNA was expressed in many tissues of the pig:higher in the stomach,kidney,lung,small intestine and ovary,and lower in the heart,spleen and large intestine.The OPN protein size differed in different tissues:70 kDa in liver and muscle,70 and 45 kDa in stomach,small intestines and kidney,70,45 and 24 kDa in lung,heart and uterus.
基金The project was supported by the National Natural Science Foundation of China(39830290).
文摘In present study, the developmental patterns of somatostatin (SS) gene expression in gastric tissue were compared between Erhualian and Large White pigs. A semi-quantitative RT-PCR was applied to determine the levels of SS mRNA. The results indicated that: (1) The gastric SS mRNA expression was high at birth, followed by a significant decrease (P<0. 05) at 3 days of age in both breeds of pigs; (2) From 3 to 30 days of age, the expression of SS mRNA in gastric tissue exhibited remarkable up-regulation in both breeds, after which a strain difference in the developmental pattern was observed. In Erhualian pigs, SS mRNA expression reached a peak at 90 days of age, declined thereafter towards 180 days of age. In Large White pigs, however, the expression of SS mRNA remained constant from 30 days of age onwards; (3) In general, Erhualian pigs expressed higher levels of SS mRNA in gastric tissue compared with Large White pigs at the same age. The strain difference was significant from birth to 90 days of age, but vanished at 120 and 180 days of age. The results suggest that the gastric expression of SS in the pig is regulated following an instinct timetable in a strain-specific manner, its relationship with the development of gastric function as well as its interactions with environmental factors are to be elucidated.
文摘The security of civil engineering is an important task due to the economic, social and environmental significance. Compared with conventional sensors, the optical fiber sensors have their unique characteristics.Being durable, stable and insensitive to external perturbations,they are particular interesting for the long-term monitoring of civil structures.Focus is on absolute measurement optical fiber sensors, which are emerging from the monitoring large structural, including SOFO system, F-P optical fiber sensors, and fiber Bragg grating sensors. The principle, characteristic and application of these three kinds of optical fiber sensors are described together with their future prospects.
文摘为研究胎次和配种季节对长大二元杂交母猪繁殖性能的影响,本试验选取了11333窝长大二元杂交母猪的产仔数据,包括总产仔数(Total number born,TNB)、产活仔数(Number born alive,NBA)、产弱仔数(Number of weak births,NWB)、仔猪出生窝重(Litter birth weight,LBW)、仔猪断奶数(Number of wearning,NW)、妊娠时间(Time to pregnancy,TTP)、产死胎数(Number of stillborn,NSB)等7个指标,分析了配种季节和胎次共同作用对长大二元杂交猪繁殖性能的影响。结果表明,4、5、6胎次母猪的总产仔数显著高于其他胎次(P<0.05),其中6胎次母猪的总产仔数最高;且4胎次母猪产活仔数显著高于其他胎次(P<0.05);2胎次母猪产弱仔数显著低于其他胎次(P<0.05);4胎次母猪所产仔猪的出生窝重显著高于其他胎次(P<0.05),第2胎母猪妊娠时间显著低于其他胎次(P<0.05),初产母猪妊娠时间显著高于其他胎次(P<0.05);发现母猪产死胎数随胎次增加而呈上升趋势,在7胎次及以上达到最高(P<0.05)。母猪的胎次与总产仔数、产活仔数、仔猪出生窝重、仔猪断奶数、产死胎数呈极显著正相关关系(P<0.01);胎次与妊娠时间呈极显著负相关关系(P<0.01)。第4胎次母猪在秋冬季配种产仔性能最好,第2胎次母猪在夏季配种产仔性能最好,5胎次母猪在春季配种产仔性能最好,第6胎次母猪在秋季配种产仔性能最好;初产母猪在秋季配种产仔性能最好,7胎次及以上母猪在春季配种产仔性能最好。胎次和季节对母猪的产仔性能及仔猪生产性能的影响研究,可以为养猪企业合理利用母猪年限提供数据参考。
