Large animal models for Huntington's disease research

Huntington'sdisease(HD)isahereditary neurodegenerative disorder for which there is currently no effectivetreatmentavailable.Consequently,the development of appropriate disease models is critical to thoroughly investigate disease progression.The genetic basis of HD involves the abnormal expansion of CAG repeats in the huntingtin(HTT)gene,leading to the expansion of a polyglutamine repeat in the HTT protein.Mutant HTT carrying the expanded polyglutamine repeat undergoes misfolding and forms aggregates in the brain,which precipitate selective neuronal loss in specific brain regions.Animal models play an important role in elucidating the pathogenesis of neurodegenerative disorders such as HD and in identifying potential therapeutic targets.Due to the marked species differences between rodents and larger animals,substantial efforts have been directed toward establishing large animal models for HD research.These models are pivotal for advancing the discovery of novel therapeutic targets,enhancing effective drug delivery methods,and improving treatment outcomes.We have explored the advantages of utilizing large animal models,particularly pigs,in previous reviews.Since then,however,significant progress has been made in developing more sophisticated animal models that faithfully replicate the typical pathology of HD.In the current review,we provide a comprehensive overview of large animal models of HD,incorporating recent findings regarding the establishment of HD knock-in(KI)pigs and their genetic therapy.We also explore the utilization of large animal models in HD research,with a focus on sheep,non-human primates(NHPs),and pigs.Our objective is to provide valuable insights into the application of these large animal models for the investigation and treatment of neurodegenerative disorders.

Association of polymorphisms of Nramp1 gene with immune function and production performance of large white pig

The present research was designed to study the association of polymorphism of natural resistance-associated macrophage proteinl （Nrampl） with some immune function and the production performance in Large White pig. The PCR-RFLP technique was applied to analyze the correlation between the polymorphisms of Nrampl gene and immune function [value of Polymorphonuclear Leukocytes （PMN） obtained by Nitroblue Tetrazolium （NBT） Reduction and effect of Cytotoxin in Monocyte] and production performance in 165 Large White pigs. The results showed that there was one Nde I restriction locus in Large White pig, and both values of PMN by NBT Reduction and effect of Cytotoxin in Monocyte in genotype BB were higher than those in genotype AB （P〈0.05）. Simultaneously, the weight of 180-day-old pigs with genotype BB was higher than that with genotype AB （P〈0.05）. The results indicated that there was a significant correlation between different genotypes of Nrampl gene and Immune function and production performance, and it can be regarded as a candidate gene of disease resistance. All these results provide valuable reference to further studies of pig disease resistance.

Cloning,Expression and Functional Analysis of Osteopontin Gene in Large White Pig

The full-length cDNA of 909 bp of the osteopontin gene(OPN) was isolated from of Large White Pig and analyzed with bioinformatics methods.The results showed high proportions of Asp,Glu and Ser and verified presence of the special sequence Arg-Gly-Asp(RGD) in the primary structure of OPN.There were high proportions of alpha helices and strong hydrophilicity in the secondary structure. The signature sequence of OPN([KQ]-x-[TA]-x(2)-[GA]-S-S-E-E-K) was located in the first region of high homology.Two phylogenetic trees were constructed,based on the entire OPN protein sequence and the conserved signature sequence,and showed that the relationship between pig and cow was the closest, but farthest between pig and chicken.OPN mRNA was expressed in many tissues of the pig:higher in the stomach,kidney,lung,small intestine and ovary,and lower in the heart,spleen and large intestine.The OPN protein size differed in different tissues:70 kDa in liver and muscle,70 and 45 kDa in stomach,small intestines and kidney,70,45 and 24 kDa in lung,heart and uterus.

The Developmental Patterns of Somatostatin Gene Expression in Gastric Tissue of Erhualian and Large White Pigs

In present study, the developmental patterns of somatostatin (SS) gene expression in gastric tissue were compared between Erhualian and Large White pigs. A semi-quantitative RT-PCR was applied to determine the levels of SS mRNA. The results indicated that: (1) The gastric SS mRNA expression was high at birth, followed by a significant decrease (P<0. 05) at 3 days of age in both breeds of pigs; (2) From 3 to 30 days of age, the expression of SS mRNA in gastric tissue exhibited remarkable up-regulation in both breeds, after which a strain difference in the developmental pattern was observed. In Erhualian pigs, SS mRNA expression reached a peak at 90 days of age, declined thereafter towards 180 days of age. In Large White pigs, however, the expression of SS mRNA remained constant from 30 days of age onwards; (3) In general, Erhualian pigs expressed higher levels of SS mRNA in gastric tissue compared with Large White pigs at the same age. The strain difference was significant from birth to 90 days of age, but vanished at 120 and 180 days of age. The results suggest that the gastric expression of SS in the pig is regulated following an instinct timetable in a strain-specific manner, its relationship with the development of gastric function as well as its interactions with environmental factors are to be elucidated.

Comparison of Muscle Fiber Growth from F1Generations of Large Yorkshire Pig andErhualian Pig by Reciprocal Cross

[ Objective] To compare the muscle fiber growth between F1 generations of large Yorkshire pig and Erhualian pig by reciprocal cross, and provide a theoretical basis for the integrated breeding of meat quality traits and growth performances by rational utilization of hybrid combination. [ Method] The hybrid pigs of large Yorkshire pig and Erhualian pig were fed and managed in the same conditions. The reciprocal combinations were Erhualian pig ♀ × Yorkshire pigd, and Yorkshire pig ♀ × Erhualian pig♂ , respectively. At the age of 20, 70, 120 and 180 d, the Iongissimus dorsi muscle and thigh muscle were collected and made into frozen sections for hematoxylin and eosin （HE） staining, and then the muscle fiber area was determined. [ Result] The muscle fiber was thickened gradually with increasing age; and the absolute growth curve of muscle fiber area was presented as ＂S＂ shape. The diameter, perimeter and area of Iongissimus dorsi muscle and thigh muscle fibers in the reciprocal combination of Yorkshire pig ♀ × Erhualian pig♂ were higher than those in the reciprocal combination of Erhualian pig ♀ × Yorkshire pig♂ （P 〈 0.01 ）. The thigh muscle grew faster than Iongissimus dorsi muscle at the age of 20 -120 d; but at the age of 120 -180 d, the growth rate of Iongissimus dorsi muscle was higher than that of thigh muscle; and at the age of 180 d, the muscle fiber area had no significant difference between the Iongissimus dorsi muscle and the thigh muscle. The muscle fiber area had extremely significant difference between the reciprocal combinations at the age of 20 d; but no significant difference was found at the age of 180 d. [ Conclusion] The reciprocal combinations can affect the muscle fiber growth of hybrid pigs of Yorkshire pig and Erhualian pig, but the affect degree reduces with the increasing age.

A combined pig model to determine the net absorption of volatile fatty acids in the large intestine under different levels of crude fiber

Background:This study aimed to develop a combined model to quantify the net absorption of volatile fatty acids(VFA)in the large intestine(LI)of pigs.Methods:Fifteen female growing pigs(Duroc×Large White×Landrace)were ranked by body weight(30±2.1 kg)on day 0 and assigned to one of three treatments,namely the basal diet containing different crude fiber(CF)levels(LCF:3.0%CF,MCF:4.5%CF,and HCF:6.0%CF).The pigs were implanted with the terminal ileum fistula and the cannulation of the ileal mesenteric vein(IMV),portal vein(PV),and left femoral artery(LFA)from days 6 to 7.[13 C]-Labeled VFA and P-aminohippuric acid were constantly perfused into the terminal ileum fistula and the cannulation of the IMV(day 15),respectively.Blood samples were collected from the PV and the LFA during perfusion(5 h),and LI samples were collected.Results:The net flux of[12 C]-acetic acid in the PV was greater for LCF versus MCF(p=0.045),but no difference was observed in the net flux of[12 C]-propionic acid(p=0.505)and[12 C]-butyric acid(p=0.35)in the PV among treatments.The deposition of[12 C]-acetic acid in the LI was greater for LCF versus MCF(p=0.014),whereas the deposition of[12 C]-propionic acid(p=0.007)and[12 C]-butyric acid(p=0.037)in the LI was greater for LCF versus HCF.Conclusions:In conclusion,this pig model was found conducive to study the net absorption of VFAs in the LI,and LCF had more net absorption of VFAs in the LI than MCF and HCF.

The pig as a preclinical traumatic brain injury model:current models,functional outcome measures,and translational detection strategies

Traumatic brain injury(TBI) is a major contributor of long-term disability and a leading cause of death worldwide. A series of secondary injury cascades can contribute to cell death, tissue loss, and ultimately to the development of functional impairments. However, there are currently no effective therapeutic interventions that improve brain outcomes following TBI. As a result, a number of experimental TBI models have been developed to recapitulate TBI injury mechanisms and to test the efficacy of potential therapeutics. The pig model has recently come to the forefront as the pig brain is closer in size, structure, and composition to the human brain compared to traditional rodent models, making it an ideal large animal model to study TBI pathophysiology and functional outcomes. This review will focus on the shared characteristics between humans and pigs that make them ideal for modeling TBI and will review the three most common pig TBI models–the diffuse axonal injury, the controlled cortical impact, and the fluid percussion models. It will also review current advances in functional outcome assessment measures and other non-invasive, translational TBI detection and measurement tools like biomarker analysis and magnetic resonance imaging. The use of pigs as TBI models and the continued development and improvement of translational assessment modalities have made significant contributions to unraveling the complex cascade of TBI sequela and provide an important means to study potential clinically relevant therapeutic interventions.

Higher Tolerance of Min Pigs Under Cold Stress:An Assessment from Reproduction Perspective

Raising Min pigs(MPs)in northern China is challenging,because of harsh environmental conditions in winter.There is a hypothesis that MPs can tolerate cold stress better than Large White pigs(LWPs)and their oocyte competence is particularly protected.Over a period of 3 years,multiple cohorts of MPs and LWPs under different environmental conditions were studied in this paper.Follistatin(FST)serum level was used as a marker for swine oocyte competence.Oocytes were collected from the swine and their maturation rate,cleavage rate and blastocyst rate were measured.Lastly,mRNA expression in ovary and longissimus dorsi for the genes FST,luteinizing hormone receptor(LHR)and cold-inducible RNA-binding protein(CIRP)was measured.Under cold stress,serum follistatin concentrations of both LWPs and MPs were markedly increased,and they were more in LWPs than in MPs.However,oocyte competence was found to be unaffected by cold stress in MPs.It was found that in a separate group of LWPs,oocyte competence was lower in samples collected in winter than that in other seasons.Interestingly,follistatin levels in the follicular fluid of MPs showed no change with cold stress,and serum follistatin levels were increased in sows.Gene expression analysis showed a significant reduction in FST,LHR and CIRP expressions under cold stress in longissimus dorsi,but not in the ovaries.It was found that MPs were better able to maintain oocyte competence under cold stress,which might be due to improved regulation of follistatin in follicular fluid,and MPs appeared likely an adaptation to the harsh conditions that they faced under domestication in northern China.

Effects of Addition of Amino Acids or Soybean Phospholipid in Diet on Slaughter Performance and Meat Quality of Pigs

[Objectives]The study aims to discuss the effects of addition of arginine and glutamic acid or soybean phospholipid,vitamin E and yeast selenium in diet on the slaughter performance and meat quality of long(white)×large(York)binary hybrid pigs.[Methods]27 long×large castrated hybrid boars with the body weight of(54.4±0.15)kg were randomly divided into 3 groups,with 3 replicates per group and 3 pigs per replicate.Group A was the control group,in which the pigs were fed basal diet;in group B,0.8%arginine and 0.60%glutamate were added to the basal diet;in group C,75 g of soybean phospholipid,20 g of vitamin E and 8 g of yeast selenium were added to every 100 kg of the basal diet.The trial period was 60 d.After the experiment was ended,one test pig with similar body weight was selected from each replicate for slaughter and meat determination.[Results]The average weight gain and eye muscle area of the pigs in group B were significantly higher than those in group C(P<0.05),and also showed an increasing trend compared with group A,but there was no statistically significant difference(P>0.05);there was no significant difference between group B or C and group A in the average weight gain and eye muscle area(P>0.05).There was no significant difference in other slaughter performance between the three groups(P>0.05).Besides,there was also no significant difference in the content of various amino acids,total amino acids and total umami amino acids between the three groups(P>0.05).The inosine content in the longissimus dorsi muscle and muscle cooking loss of binary hybrid pigs in group C were significantly better than those in group B(P<0.05),and also had a tendency to be better than those in group A,but there was no significant difference(P>0.05);there was no significant difference between group B or C and group A in the inosine content and muscle cooking loss of the pigs(P>0.05).In addition,there was no significant difference in other meat traits and chemical composition of the longissimus dorsi muscle between group B or C and group A(P>0.05).[Conclusions]The addition of arginine and glutamic acid or soybean phospholipid,vitamin E and yeast selenium in diet had no significant effect on the growth rate,slaughter performance and meat traits of long×large binary hybrid pigs.

Research Progress on Energy Plants in Piggery Wastewater Treatment

Large-scale pig-raising can discharge a great deal of wastewater,which contains high content of organic matter,ammonia nitrogen and suspended solids.The improper treatment of the piggery wastewater can lead to serious environmental problems. As a liquid fertilizer,piggery wastewater is relatively low in fertilizer efficiency and high in transportation cost,so it is very necessary to treat it in situ. Energy plants have the advantages of rapid growth,large biomass,strong tillering ability and developed root system. Therefore,energy plants can be used to absorb and transform the pollutants( like nitrogen and phosphorus) in piggery wastewater into the components of plants,as well as form the rhizosphere environment which is conducive to microbial growth,so as to enhance the effects of nitrogen and phosphorus removal. The obtained energy plants can be recycled as the raw materials for biogas to increase the production of biogas,which brings economic benefits while solving the environmental problems caused by piggery wastewater.

规模化猪场粪污处理利用现状及案例分析

规模化猪场在养殖过程中会产生大量的粪污,如果不加以妥善处理与利用,将对环境和公共健康造成严重威胁。粪污处理与资源化利用成为实现养猪业可持续发展的关键环节。本文探讨了规模化猪场粪污的危害,介绍了规模化猪场粪污集中收集系统及目前规模化猪场在猪舍粪污处理中使用的尿泡粪和机械清粪2种清粪工艺,并详细讨论了生物发酵、厌氧消化、好氧处理等多种方法对固体粪污和液体污水的处理技术,以满足不同规模化养猪场的清粪需求。同时,探讨了规模化猪场种养结合生态循环利用和工业化深度处理2种主要的粪污处理利用模式。展望了未来科技创新对粪污处理与利用的推动作用,呼吁各方共同努力,以实现养猪企业的可持续发展、保障环境和人类健康的计划。本文为规模化养猪场选择粪污处理技术和利用模式提供了有益的参考,有助于环境保护和养猪业可持续发展。

小型猪气管插管时异氟醚半数有效剂量的探索

目的本研究拟探讨小剂量舒泰(1.5 mg/kg)肌注复合异氟烷吸入用于保留自主呼吸的小型猪气管插管时50%和95%有效的异氟烷最小肺泡气浓度(50%and 95%minimum alveolar concentrations effective inhaled,MAC EI50and MAC EI95),为小型猪提供安全的麻醉插管方法。方法44例全身麻醉的巴马小型猪,肌注舒泰镇静后,面罩吸入异氟烷进行麻醉诱导,监测心率、血压、呼吸、体温、氧饱和度、呼末二氧化碳浓度和呼末异氟烷浓度。初始呼末异氟烷浓度设置为2.0%,采用Cooper’s评分评价气管插管满意度,采用Dixon’s改良序贯法测算剂量-效应关系。Probit回归法计算MAC EI50和MAC EI95及95%可信区间(confidence interval,CI)。结果小剂量舒泰复合异氟烷吸入诱导直接喉镜气管插管时,小型猪肺泡气浓度的MAC EI503.10%(95%CI 2.79%~3.56%),MAC EI953.77%(95%CI 3.41%~6.42%);在做好监测和气道管理预案的基础上,肺泡异氟烷浓度3.10%~3.75%,均能保证小型猪生命体征稳定。结论舒泰复合异氟烷吸入用于保留自主呼吸的小型猪气管插管是建立小型猪经口气道较好且安全的麻醉方法,具有推广价值。

大白猪生长、繁殖和体尺性状遗传参数估计

旨在对法系大白猪生长、繁殖和体尺性状进行遗传参数估计,为育种实践中目标性状选择及指数模型权重系数设定提供数据支撑。本研究收集了黑龙江某原种猪场2020—2023年法系大白猪生长性状信息8 751条,繁殖性状信息12 762条,体尺性状信息7 407条。使用DMU软件,基于动物模型估计法系大白猪生长性状达100 kg体重日龄(D100)、100 kg日增重(DG100)、100 kg体重活体背膘厚(BF100)、100 kg体重眼肌面积(LMA100);繁殖性状总产仔数(TNB)、产活仔数(NBA)、健仔数(NHB)、初生窝重(LBW);体尺性状体长(BL)、体高(BH)、管围(CBC)的遗传参数。结果表明:大白猪生长性状(D100、DG100、BF100、LMA100)遗传力分别为0.232、0.220、0.334、0.390,均属于中等遗传力性状;繁殖性状(TNB、NBA、NHB、LBW)遗传力分别为0.081、0.065、0.060、0.090,均属于低遗传力性状;体尺性状(BL、BH、CBC)遗传力分别为0.677、0.877、0.227,BL、BH属于高遗传力性状,CBC属于中等遗传力性状。大白猪生长性状D100与DG100之间的遗传相关与表型相关分别为-0.999、-0.994,呈现出较强的负相关。繁殖性状的遗传相关和表型相关均呈正相关,NBA与NHB的遗传相关和表型相关均最高,分别为0.995和0.977。体尺性状之间的遗传相关和表型相关分别在-0.345~0.246和-0.057~0.125,BL与BH之间的遗传相关与表型相关均为负相关,且表型相关程度不高。以上结果表明,在育种实践中需要针对上述生长性状、繁殖性状和体尺性状遗传参数的不同特点,制定合理的育种方案,本研究估计的大白猪群体遗传参数,可以作为制定育种方案的依据,提高育种工作效率。

生猪规模养殖粪污资源化利用不同模式效益及其影响因素研究——基于湖北省襄阳市襄州区的实地调研

为了分析襄阳市襄州区生猪规模养殖场粪污资源化利用不同模式的效益及其影响因素,本研究运用熵值法从经济、社会、生态和技术4个维度13项指标测算生猪规模养殖粪污资源化利用模式评价指标权重;运用TOPSIS法建立生猪规模养殖粪污资源化利用模式综合评价模型,分析各模式综合效益;运用Tobit回归模型从养殖场主的基本特征、养殖场的经营状况和外部社会环境3个方面探究不同生猪粪污资源化利用模式综合效益的影响因素。结果表明:准则层中经济效益、社会效益、生态效益和技术效益的权重分别为0.337 7,0.360 8,0.223 8,0.077 7,其中社会效益权重最大,然后依次是经济效益、生态效益、技术效益。指标层中带动就业、粪污资源化收益和种养平衡率三个指标权重较大,分别为0.329 1,0.299 7,0.170 0。中、小规模养殖场采用沼气发酵模式综合效益最高,大规模养殖场采用综合利用模式综合效益最高。养殖收入、养殖规模和配套耕地面积对粪污资源化利用模式综合效益影响显著,其中养殖收入、配套耕地面积与粪污资源化利用模式综合效益呈正相关关系,养殖规模与粪污资源化利用模式综合效益呈负相关关系。据此,笔者提出加快研发粪污处理和利用相关技术,改进粪污资源化利用模式;强化种养循环发展模式,大力推行种养结合;培育第三方服务主体,解决中、小规模养殖场粪污利用问题;加大政府扶持力度,完善政府激励等措施。

计算机视觉技术在规模化猪场应用的研究进展

作为人工智能的一个主要分支,计算机视觉技术在许多领域取得了很大的成就,其在规模化猪场中的应用将为猪场带来产业性的变革。计算机视觉技术可以大幅度减少规模化猪场中人员工作量,同时使猪场对生猪的相关判断更加准确。本文简述了规模化猪场的现状和计算机视觉技术,重点综述了计算机视觉技术在规模化猪场中对猪只的行为监测、疾病预测、情感状态分析、猪只判别、生产管理、生物安全和防疫以及企业的落地探索等方面的研究进展,以期为促进我国规模化猪场的现代化和智能化发展提供参考。

“猛香系”杜长大三元猪相关屠宰性能、肉质测定的分析

为充分研究家育“猛香系”杜×长×大三元猪的屠宰性能及肉品质,对其进行屠宰实验及品质检验。选择30头同一饲养环境、管理条件的体重约为115 kg三元猪进行屠宰测定,分析其屠宰性能、胴体性状以及肉品品质。结果表明:胴体指标分析得出,胴体均重为85.25 kg,屠宰率为70.25%,胴体长为97.45 cm,背膘厚为23.59 mm,皮厚为2.66 mm,眼肌面积为49.31 cm^(2),腿臀比例为33.19%;肉质指标分析得出,肌肉色值L_(24)42.93,pH_(1)值6.37,pH_(24)值为5.76,48 h滴水损失2.53%,系水力92.58%。实验为进一步提高和改善三元猪的胴体品质和优质选育育种方案提供参考的数据支撑。

一例规模化猪场流行性腹泻病的诊断与防控风险分析

2022年2月4日,广西壮族自治区某规模化猪场妊娠母猪陆续出现水样腹泻、不吃或少吃料的临床症状,2022年2月6日,产房仔猪出现呕吐、水样腹泻,怕冷扎堆,脱水严重,且传播速度快。通过试剂盒快速检测和实验室检测,诊断为猪流行性腹泻病。针对检测结果对猪群进行紧急疫苗接种和药物治疗,并对此次疫情的发生和发展过程进行防控风险点分析,发现养猪场在生物安全方面存在较大的风险点,通过整改并采取严格的生物安全管理措施后,母猪群发病情况得到缓解,仔猪腹泻窝数下降,猪群趋于稳定,生产成绩逐步恢复。通过对此次案例的分析探讨,为规模化猪场对猪流行性腹泻病的发现和防控提供临床经验,同时增强对流行性腹泻病的风险预警意识,健全规模化猪场对流行性腹泻病的风险预警机制。

An increase in corn resistant starch decreases protein fermentation and modulates gut microbiota during in vitro cultivation of pig large intestinal inocula

High-protein diet could cause an increase in protein fermentation in the large intestine, leading to an increased production of potentially detrimental metabolites. We hypothesized that an increase in corn resistant starch content may attenuate the protein fermentation. The aim of this study was to evaluate the effect of resistant starch on protein fermentation by inocula from large intestine of pigs using in vitro cultivation. Fermentation patterns were analyzed during a 24-h incubation of cecal and colonic digesta with varying corn resistant starch contents, using casein protein as sole nitrogen source. The results showed that the concentration of short-chain fatty acids(SCFA) and cumulative gas production were significantly increased(P < 0.05), while ammonia-nitrogen(NH_3-N) and branched-chain fatty acids(BCFA), which indicated protein fermentation, decreased when the corn resistant starch levels increased(P < 0.05). The copies of total bacteria, Bifidobacterium and Lactobacillus were significantly increased with the increased corn resistant starch levels after incubation(P < 0.05). The copies of the Bifidobacterium and Lactobacillus in cecum were significantly higher than those in colon(P < 0.05). We conclude that the addition of corn resistant starch weakens the protein fermentation by influencing microbial population and reducing protein fermentation in the cecum and colon in vitro.

法系大白猪繁殖性状遗传参数估计及遗传进展分析

旨在对北京市某母猪场法系大白猪繁殖性状进行遗传参数估计,评估其遗传进展,为该育种场制定合理的育种方案提供理论依据。本研究共收集北京市某母猪场2016-2022年法系大白母猪原始配种记录29 269条,原始分娩记录20 770条,原始系谱记录146 871条,估计该公司法系大白母猪繁殖性状的遗传参数,采用基于动物模型的最佳线性无偏预测(best linear unbiased prediction, BLUP)方法,利用DMU软件使用单性状和双性状重复力模型进行遗传力、遗传相关、表型相关和育种值的估计。结果显示,本研究中法系大白猪TNB为12.67头,NBA为10.90头,NSB为1.35头,MUM为0.14头,LWT为13.99 kg, LW21为73.93 kg;繁殖性状遗传力估计值范围为0.017~0.134,属于低遗传力;繁殖性状间的遗传相关在-0.368~0.856之间,表型相关在-0.213~0.847之间;通过绘制繁殖性状表型趋势图及遗传趋势图发现,个体的繁殖性状表型值呈上升趋势,育种值呈先上升后下降趋势。本研究结果表明,该群体遗传改良取得了良好的进展,研究结果可为法系大白猪的品种选育和遗传改良提供参考指导和理论基础。

湖南省不同品种公猪精液品质及运动性能的研究

[目的]研究不同品种公猪精液质量的差异,提高公猪饲养管理水平。[方法]收集2021年4月—2022年11月,湖南省32个生猪良补县51批次247头成年公猪(长白猪73头、大白猪75头、杜洛克猪99头)的精液质量数据,通过方差分析探讨不同品种公猪精液的活力、畸形率和精子运动参数等差异。[结果]不同品种公猪精液的活力和畸形率没有显著差异;长白猪精子的缓慢比例显著高于杜洛克猪和大白猪,长白猪精子的转圈比例显著低于杜洛克猪和大白猪,其他运动轨迹参数差异不显著。[结论]长白猪精子的迁移速率低于杜洛克猪和大白猪,长白猪前向运动精子数的比例高于杜洛克猪和大白猪。