The application model of epidemic disease assessment technology for Web-based large-scale pig farm was expounded from the identification of epidemic disease risk factors, construction of risk assessment model and deve...The application model of epidemic disease assessment technology for Web-based large-scale pig farm was expounded from the identification of epidemic disease risk factors, construction of risk assessment model and development of risk assessment system. The assessed pig farm uploaded the epidemic disease risk data information through on-line answering evaluating questionnaire to get the immediate evaluation report. The model could enhance the risk communication between pig farm veterinarian, manager and veterinary experts to help farm system understand and find disease risk factors, assess and report the potential high risk items of the pig farm in the three systems of engineering epidemic disease prevention technology, biological safety and immune monitoring, and promote the improvement and perfection of epidemic disease prevention and control measures.展开更多
Large-scale breeding of livestock and poultry is the main way of modern animal husbandry development.This breeding method is widely used by the majority of owners because of its easy technical promotion,low breeding c...Large-scale breeding of livestock and poultry is the main way of modern animal husbandry development.This breeding method is widely used by the majority of owners because of its easy technical promotion,low breeding cost and good economic benefits.But with the development of large-scale livestock and poultry breeding,the pollution and damage to the environment caused by fecal pollution from large-scale livestock and poultry breeding has been criticized by many environmentalists.In this paper,through visit and investigation of large-scale livestock and poultry farms,the methods and measures of environmental protection in the construction of large-scale livestock and poultry farms are proposed.展开更多
Several studies have been done to isolate probiotic bacteria from different sources. In this present study, an attempt was made to isolate, screen and identify potential probiotic bacteria from cattle farm soil in Dib...Several studies have been done to isolate probiotic bacteria from different sources. In this present study, an attempt was made to isolate, screen and identify potential probiotic bacteria from cattle farm soil in Dibrugarh district, Assam, India. At the level of screening, the result showed the isolates designated as DUA4, DUD3 and DUE2 showed a percent survival rate of 75.36, 69.14 and 52.36 respectively at a pH of 2.5. Similarly survival rate of the same isolates in 0.5% bile salt condition was found to be 117.17%, 144.59% and 118.10% for the isolates DUA4, DUD3 and DUE2 respectively. Antimicrobial activity of the isolates towards the indicator organisms tested showed that DUA4 inhibited gram positive organisms while DUD3 showed activity against both gram positive and gram negative bacteria. All the three isolates showed activity against L. monocytogenes. Autoaggregation ability of the isolates DUA4, DUD3 and DUE2 was found to be 44.15%, 54.11% and 9.42% respectively. The adhesion ability of the isolates DUD3, DUA4 and DUE2 to xylene was 61.78%, 45.37% and 14.83% respectively. Antimicrobial susceptibility test of the isolates showed that the isolates are in general sensitive to antibiotics tested. Phenotypic and genotypic analysis based on the 16S rRNA gene sequence of the isolates DUA4, DUD3 and DUE2 resulted in the identification and designation of the isolate DUA4 as Bacillus spp., DUD3 as Enterococcus faecium and DUE2 as Enterobacter sp. In conclusion, the study has indicated the possibility of isolating potential probiotic bacterial strains from cattle farm soil.展开更多
The aim of the present study was to evaluate the impact of the technological level in small-scale dual-purpose cow-calf <em>Bos indicus</em> female operations on the resumption of the ovarian activity post...The aim of the present study was to evaluate the impact of the technological level in small-scale dual-purpose cow-calf <em>Bos indicus</em> female operations on the resumption of the ovarian activity post-partum. A total of 13 small scale dual-purpose <em>Bos indicus</em> × <em>Bos taurus</em> operations were included in this experiment and they were classified according to their technological status as: high technological status (HT), medium technological status (MT) and low technological status (LT). Ninety-three mature cows were treated with either a CIDR (controlled internal drug release device) with estradiol benzoate (CIDR+EB) or alone (CIDR). At day 9 all animals received intravaginally a CIDR for nine days. At day 0, the CIDR was withdrawn and 24 hours later one dose of estradiol benzoate was administered to 51 cows (17 in HT, 17 in MT and 17 in LT), the remaining animals (n = 42) did not receive the estradiol benzoate administration (14 in HT, 13 in MT and 15 in LT). Comparisons were performed on serum progesterone concentrations after CIDR treatment. By day 7 after implant withdrawal, the proportion of cows that resume ovarian activity detected by progesterone concentration increased in all technological level farms, no statistical main effect was observed within technological level. By day 17 there was a significant main effect of technological level (P = 0.05) on the proportion of animal that resumed the estrous cycles, but neither a main effect of treatment (P = 0.97) nor an interaction between technological level and treatment (P = 0.98). Furthermore, technological level of the farm showed a tendency (P = 0.07) to affect that resume the estrous activity, and a significant effect of treatment was observed (P = 0.005) but no interaction between technological level and treatment. The proportion of cows that showed estrus was different across technological level (P = 0.02), the highest proportion of cows showing estrus regardless of treatment was in the HT: 90% (40% CIDR and 50% CIDR+EB), MT: 50% (13.3% CIDR and 36.7% CIDR+EB) and in the LT: 50% (18.8% CIDR and 31.3% CIDR+EB). In conclusion, the combination of a progestogen and estradiol benzoate resulted in a high proportion of cows that were induced to resume the ovarian activity and this treatment was particularly beneficial in the medium and low technological status of the farms.展开更多
Large-scale farming by agricultural land transfers has been increasingly promoted in recent years,but the possible impacts on crop production,especially cash crops,and soil acidification remain unclear.This study obta...Large-scale farming by agricultural land transfers has been increasingly promoted in recent years,but the possible impacts on crop production,especially cash crops,and soil acidification remain unclear.This study obtained data for 110 banana plantations in Long’an County,China,and categorized them into small(<0.67 ha),medium(0.67−6.7 ha),and large(>6.7 ha)to determine banana cultivation,nutrient management,and soil acidification rates on farms of the three sizes.Banana yield per unit area significantly increased with increased farm size,and large farms had the highest average yield(48.9 t·ha^(−1))with the least variation.Despite a significant increase in organic fertilizer and base cation inputs,nitrogen(N)surplus did not differ significantly with increasing farm size.With large farms,actual soil acidification rate was significantly lower by 19.1 to 24.0 keq·ha^(−1)·yr^(−1);however,potential soil acidification rate increased with increased overuse of phosphorus.Overall,larger banana plantations used fewer mineral N fertilizers reducing the rate of soil acidification and increasing the H+buffering provided by organic fertilizers.It is concluded that larger farms deliver the dual benefits of higher,less variable banana yield and mitigation of soil acidification by substituting organic N for mineral N fertilizers,supporting sustainable soil management and food production.展开更多
基金Supported by the Fund Program of Jiangsu Academy of Agricultural Sciences(6111689)the Planning Program of"the Twelfth Five-year-plan"in National Science and Technology for the Rural Developme+nt in China(2015BAD12B04-1.2)the Fund for Independent Innovation of Agricultural Science and Technology of Jiangsu Province[CX(16)1006]~~
文摘The application model of epidemic disease assessment technology for Web-based large-scale pig farm was expounded from the identification of epidemic disease risk factors, construction of risk assessment model and development of risk assessment system. The assessed pig farm uploaded the epidemic disease risk data information through on-line answering evaluating questionnaire to get the immediate evaluation report. The model could enhance the risk communication between pig farm veterinarian, manager and veterinary experts to help farm system understand and find disease risk factors, assess and report the potential high risk items of the pig farm in the three systems of engineering epidemic disease prevention technology, biological safety and immune monitoring, and promote the improvement and perfection of epidemic disease prevention and control measures.
文摘Large-scale breeding of livestock and poultry is the main way of modern animal husbandry development.This breeding method is widely used by the majority of owners because of its easy technical promotion,low breeding cost and good economic benefits.But with the development of large-scale livestock and poultry breeding,the pollution and damage to the environment caused by fecal pollution from large-scale livestock and poultry breeding has been criticized by many environmentalists.In this paper,through visit and investigation of large-scale livestock and poultry farms,the methods and measures of environmental protection in the construction of large-scale livestock and poultry farms are proposed.
文摘Several studies have been done to isolate probiotic bacteria from different sources. In this present study, an attempt was made to isolate, screen and identify potential probiotic bacteria from cattle farm soil in Dibrugarh district, Assam, India. At the level of screening, the result showed the isolates designated as DUA4, DUD3 and DUE2 showed a percent survival rate of 75.36, 69.14 and 52.36 respectively at a pH of 2.5. Similarly survival rate of the same isolates in 0.5% bile salt condition was found to be 117.17%, 144.59% and 118.10% for the isolates DUA4, DUD3 and DUE2 respectively. Antimicrobial activity of the isolates towards the indicator organisms tested showed that DUA4 inhibited gram positive organisms while DUD3 showed activity against both gram positive and gram negative bacteria. All the three isolates showed activity against L. monocytogenes. Autoaggregation ability of the isolates DUA4, DUD3 and DUE2 was found to be 44.15%, 54.11% and 9.42% respectively. The adhesion ability of the isolates DUD3, DUA4 and DUE2 to xylene was 61.78%, 45.37% and 14.83% respectively. Antimicrobial susceptibility test of the isolates showed that the isolates are in general sensitive to antibiotics tested. Phenotypic and genotypic analysis based on the 16S rRNA gene sequence of the isolates DUA4, DUD3 and DUE2 resulted in the identification and designation of the isolate DUA4 as Bacillus spp., DUD3 as Enterococcus faecium and DUE2 as Enterobacter sp. In conclusion, the study has indicated the possibility of isolating potential probiotic bacterial strains from cattle farm soil.
文摘The aim of the present study was to evaluate the impact of the technological level in small-scale dual-purpose cow-calf <em>Bos indicus</em> female operations on the resumption of the ovarian activity post-partum. A total of 13 small scale dual-purpose <em>Bos indicus</em> × <em>Bos taurus</em> operations were included in this experiment and they were classified according to their technological status as: high technological status (HT), medium technological status (MT) and low technological status (LT). Ninety-three mature cows were treated with either a CIDR (controlled internal drug release device) with estradiol benzoate (CIDR+EB) or alone (CIDR). At day 9 all animals received intravaginally a CIDR for nine days. At day 0, the CIDR was withdrawn and 24 hours later one dose of estradiol benzoate was administered to 51 cows (17 in HT, 17 in MT and 17 in LT), the remaining animals (n = 42) did not receive the estradiol benzoate administration (14 in HT, 13 in MT and 15 in LT). Comparisons were performed on serum progesterone concentrations after CIDR treatment. By day 7 after implant withdrawal, the proportion of cows that resume ovarian activity detected by progesterone concentration increased in all technological level farms, no statistical main effect was observed within technological level. By day 17 there was a significant main effect of technological level (P = 0.05) on the proportion of animal that resumed the estrous cycles, but neither a main effect of treatment (P = 0.97) nor an interaction between technological level and treatment (P = 0.98). Furthermore, technological level of the farm showed a tendency (P = 0.07) to affect that resume the estrous activity, and a significant effect of treatment was observed (P = 0.005) but no interaction between technological level and treatment. The proportion of cows that showed estrus was different across technological level (P = 0.02), the highest proportion of cows showing estrus regardless of treatment was in the HT: 90% (40% CIDR and 50% CIDR+EB), MT: 50% (13.3% CIDR and 36.7% CIDR+EB) and in the LT: 50% (18.8% CIDR and 31.3% CIDR+EB). In conclusion, the combination of a progestogen and estradiol benzoate resulted in a high proportion of cows that were induced to resume the ovarian activity and this treatment was particularly beneficial in the medium and low technological status of the farms.
基金funded by Major Science and Technology Project of Yunnan Province(202102AE090053-2)National Natural Science Youth Foundation of China(41907142)Natural Science Foundation of Hainan Province(422MS095)。
文摘Large-scale farming by agricultural land transfers has been increasingly promoted in recent years,but the possible impacts on crop production,especially cash crops,and soil acidification remain unclear.This study obtained data for 110 banana plantations in Long’an County,China,and categorized them into small(<0.67 ha),medium(0.67−6.7 ha),and large(>6.7 ha)to determine banana cultivation,nutrient management,and soil acidification rates on farms of the three sizes.Banana yield per unit area significantly increased with increased farm size,and large farms had the highest average yield(48.9 t·ha^(−1))with the least variation.Despite a significant increase in organic fertilizer and base cation inputs,nitrogen(N)surplus did not differ significantly with increasing farm size.With large farms,actual soil acidification rate was significantly lower by 19.1 to 24.0 keq·ha^(−1)·yr^(−1);however,potential soil acidification rate increased with increased overuse of phosphorus.Overall,larger banana plantations used fewer mineral N fertilizers reducing the rate of soil acidification and increasing the H+buffering provided by organic fertilizers.It is concluded that larger farms deliver the dual benefits of higher,less variable banana yield and mitigation of soil acidification by substituting organic N for mineral N fertilizers,supporting sustainable soil management and food production.