To study the effects of geoclimatic variables of provenances on growth phenotypes and selected plus provenances,over 3000 trees of 22-year-old Larix kaempferi were evaluated from trials established in two climate zone...To study the effects of geoclimatic variables of provenances on growth phenotypes and selected plus provenances,over 3000 trees of 22-year-old Larix kaempferi were evaluated from trials established in two climate zones for provenance selection and to explore the influence of climate variables on provenance performance.The material was replicated plantings of 84 open pollinated families from six provenances distributed in the mountains of central Japan.Provenance variation was observed in most age groups and the heritability of growth traits showed large differences.The phenotypic maximum value of height and diameter were significantly positive with altitude,and mean annual precipitation being important factors.Diameter at breast height growth was significantly negative with altitude and spring rainfall.The Ina provenance of relatively high altitudes,was well adapted to a variety of climates.Altitude might be the driving force for phenotypic and genetic variations and local adaptation.展开更多
Early selection is an important method to shorten the breeding cycle for tree species, which may differ in the time for early selection. To evaluate the early selected time for Larix kaempferi, tree height and diamete...Early selection is an important method to shorten the breeding cycle for tree species, which may differ in the time for early selection. To evaluate the early selected time for Larix kaempferi, tree height and diameter at breast height of 57 L. kaempferi clones were measured over many different growth years. The results indicated that, except for age × clone interaction for diameter at breast height (P = 0.741), there were significant differences among all variation sources (P 〈 0.01). The coefficient of phenotypic variation ranged from 14.89 to 35.65% for height and from 19.17 to 23.86% for diameter at breast height in different growth years. The repeatability of height and of diameter at breast height among clones was high, ranging from 0.6181 to 0.8531 (height) and from 0.8443 to 0.8497 (diameter at breast height), in different growth years. There were significant positive correlations between all pairs of growth traits except between height in the 2nd growth year and height in the 30th growth year; and between height in the 2nd growth year × diameter at breast height in the 30th growth year. With a comprehen- sive evaluation method and a selection ratio of 10%, L65, L1, L62, L9, L15, and L78 were selected as excellent clones in the 30th growth year. Their average values of height and diameter at breast height were 9.81 and 16.57% higher than the overall average, representing genetic gains of 6.46 and 13.99%, respectively. This study provides a theoretical foundation for the genetic improvement of L. kaempferi.展开更多
In this study, compatible taper and stem volume equations were developed for Larix kaempferi species of South Korea. The dataset was split into two groups: 80% of the data were used in model fitting and the remaining...In this study, compatible taper and stem volume equations were developed for Larix kaempferi species of South Korea. The dataset was split into two groups: 80% of the data were used in model fitting and the remaining 2o% were used for validation. The compatible MB76 equations were used to predict the diameter outside bark to a specific height, the height to a specific diameter and the stem volume of the species. The result of the stem volume analysis was compared with the existing stem volume model of Larix kaempferi species of South Korea which was developed by the Korea Forest Research Institute and with a simple volume model that was developed with fitting dataset in this study. The compatible model provided accurate prediction of the total stem volume when compared to the existing stem volume model and with a simple volume model. It is concluded that the compatible taper and stem volume equations are more convenient to use and therefore it is recommended to be applied in the Larix kaempferi species of South Korea.展开更多
Nucleotide diversity (pi) and linkage disequilibrium (LD) analysis based on SNP marker could provide a sound basis for choosing an association analysis method. Japanese larch (Larix kaempferi) is an important timber c...Nucleotide diversity (pi) and linkage disequilibrium (LD) analysis based on SNP marker could provide a sound basis for choosing an association analysis method. Japanese larch (Larix kaempferi) is an important timber coniferous tree species for pulping and papermaking, but its high lignin content has significantly restricted it application potential. In this study, the LACCASE gene, that plays an important regulatory role for lignin biosynthesis, was selected as research target. The full-length cDNA and genomic sequences of the encoding LkLAC8 gene were isolated from the LACCASE expressed sequence tags of the Japanese larch transcriptome database using the rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). The cDNA was determined to be 1940 bp, with an open reading frame (ORF, 1734 bp) that encoded a protein of 577 AA. This protein contains four highly specific Cu2+ binding sites and 11 glycosylation sites, thus belonging to the LACCASE family. The deduced protein sequence shared an 89% identity with the PtaLAC from Pinus taeda. A real-time PCR analysis showed that the LkLAC8 transcript was expressed predominantly in mature xylem, with moderate levels in the immature xylem, cambium and mature leaves, the lowest in the roots. Lastly, the genomic sequences of LkLAC8 in 40 individuals from six naturally distributed populations of Japanese larch were amplified, and a total of 201 SNPs (103 and 98 mutation types of transition and transversion, respectively) were detected; the frequency of the SNPs was 1/19 bp. Nucleotide diversity among the six populations ranged from 0.0034 to 0.0053, which suggested that there were no significant differences among the populations. The LD analysis showed that the LD level decayed rapidly within the increasing length of the LkLAC8 gene. These results implied that LD mapping and association analysis based on candidate gene may be feasible for the marker-assisted breeding of new germplasms with low lignin in Japanese larch.展开更多
Fifty-three larch interspecific hybrid clones(Larix kaempferi × L.gmelini) and their parent clones were used for growth curve analysis of height variations.The growth curves of the 55 clones were 'S'-shaped a...Fifty-three larch interspecific hybrid clones(Larix kaempferi × L.gmelini) and their parent clones were used for growth curve analysis of height variations.The growth curves of the 55 clones were 'S'-shaped and 36 exhibited similar curves as the male parent.The coefficients of the logistic models were higher than 0.943,indicating that our results were effective in the simulation of the growth curves.ANOVA analysis showed significant differences in height of different clones (P/0.01).Average date of maximum height growth was Day 173,and average duration of rapid growth lasted for 50 days.Annual average increase in height was 9.7cm d^(-1) and daily average increase was 0.2 cm.The ratio of GR to the total annual increase in height ranged from 51.2 to 68.8%,with the average being 59.8%.There was a positive correlation between k values and plant heights which benefited from the evaluation of early plant height.There was also a positive correlation between GR(growth stage),GD(plant height) and annual increase in height.These results are informative to the evaluation of the elite clone selection and provide a theoretical basis for breeding and management.展开更多
In this research, five plots are Selected based on stand storage and productivity of planted forest of Larix kaempferi (Japanese larch) as well as their relationship with factors suCh as forest age, site condition a...In this research, five plots are Selected based on stand storage and productivity of planted forest of Larix kaempferi (Japanese larch) as well as their relationship with factors suCh as forest age, site condition and stand density. Through standard plot investigation and method of trunk analysis, systematic research is conducted on storage and productivity of the planted forest and their relationship with site condition. As shown in the research, productivity of planted forest of Larix kaempferi in low latitude area is higher than that in high latitude area while stand productivity is higher in slight acid soil. Contents of potassium element in the soil have Considerable influence on productivity of the Larix kaempferi forest.展开更多
Natural volatile organic compounds(VOCs) extracted from conifers such as P. koraiensis and L. kaempferi have long been studied for their anti-oxidant, anti-proliferative, and anti-inflammatory effects. To evaluate the...Natural volatile organic compounds(VOCs) extracted from conifers such as P. koraiensis and L. kaempferi have long been studied for their anti-oxidant, anti-proliferative, and anti-inflammatory effects. To evaluate the anti-inflammatory effects of VOCs from P. koraiensis and L. kaempferi, lipopolysaccharide(LPS) was administered to generate a mouse model for inflammation by the nasal route to the lungs and intraperitoneally to the whole body. VOCs of P. koraiensis and L. kaempferi were exposed to the mice by standardized wood panels with closed system. Increased levels of serum IgE and PGE2 were observed after exposure to dexamethasone and VOCs. We further determined the expression levels of inflammatory cytokine mRNA in the LPS-induced inflammation model by the reverse transcription quantitative polymerase chain reaction. Furthermore, the levels of cyclooxygenase-2, tumor necrosis factor-α, interleukin-1β, and interleukin-13 were determined in peripheral blood mononuclear cells. Those inflammatory cytokines and the key enzyme for inflammation cyclooxygenase-2 expression in PBMCs were strongly reversed by dexamethasone and VOCs. Lung tissues after nasal LPS exposure showed increased cytokine mRNA expressions which were suppressed by treatment with dexamethasone and VOCs. Furthermore, the damage induced by LPS was attenuated by dexamethasone and VOCs. In conclusion, the results from the present study indicate that VOCs of P. koraiensis and L. kaempferi have a therapeutic potential in the treatment or prevention of local and systemic inflammation due to their immunosuppressive effects.展开更多
The paper summarized the life cycle ,environmental features and distributions of Larix origination in Japan.The time of blooming and fruiting and the ways of cone collection and seeds storage were introduced.The treat...The paper summarized the life cycle ,environmental features and distributions of Larix origination in Japan.The time of blooming and fruiting and the ways of cone collection and seeds storage were introduced.The treating methods of seeds germination and the sowing time for both Japanese larch (Larix kaempferi Carr)and Dahurian larch (Larix gmelinii var.Japonica Pilger) were discussed.展开更多
基金supported by the Special Fund for Forest Scientific Research in the Public Welfare(201504104)the General Program of National Natural Science Foundation of China(31971652)the Fundamental Research Funds for the Central Non-profit Research Institution of CAF(CAFYBB2017ZA001-4)。
文摘To study the effects of geoclimatic variables of provenances on growth phenotypes and selected plus provenances,over 3000 trees of 22-year-old Larix kaempferi were evaluated from trials established in two climate zones for provenance selection and to explore the influence of climate variables on provenance performance.The material was replicated plantings of 84 open pollinated families from six provenances distributed in the mountains of central Japan.Provenance variation was observed in most age groups and the heritability of growth traits showed large differences.The phenotypic maximum value of height and diameter were significantly positive with altitude,and mean annual precipitation being important factors.Diameter at breast height growth was significantly negative with altitude and spring rainfall.The Ina provenance of relatively high altitudes,was well adapted to a variety of climates.Altitude might be the driving force for phenotypic and genetic variations and local adaptation.
基金supported by the Innovation Project of State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University)(No.2016C02)China Postdoctoral Science Foundation(2014M561315)
文摘Early selection is an important method to shorten the breeding cycle for tree species, which may differ in the time for early selection. To evaluate the early selected time for Larix kaempferi, tree height and diameter at breast height of 57 L. kaempferi clones were measured over many different growth years. The results indicated that, except for age × clone interaction for diameter at breast height (P = 0.741), there were significant differences among all variation sources (P 〈 0.01). The coefficient of phenotypic variation ranged from 14.89 to 35.65% for height and from 19.17 to 23.86% for diameter at breast height in different growth years. The repeatability of height and of diameter at breast height among clones was high, ranging from 0.6181 to 0.8531 (height) and from 0.8443 to 0.8497 (diameter at breast height), in different growth years. There were significant positive correlations between all pairs of growth traits except between height in the 2nd growth year and height in the 30th growth year; and between height in the 2nd growth year × diameter at breast height in the 30th growth year. With a comprehen- sive evaluation method and a selection ratio of 10%, L65, L1, L62, L9, L15, and L78 were selected as excellent clones in the 30th growth year. Their average values of height and diameter at breast height were 9.81 and 16.57% higher than the overall average, representing genetic gains of 6.46 and 13.99%, respectively. This study provides a theoretical foundation for the genetic improvement of L. kaempferi.
基金the Korea Forest Service for funding this research(Project No.S211316L020130)
文摘In this study, compatible taper and stem volume equations were developed for Larix kaempferi species of South Korea. The dataset was split into two groups: 80% of the data were used in model fitting and the remaining 2o% were used for validation. The compatible MB76 equations were used to predict the diameter outside bark to a specific height, the height to a specific diameter and the stem volume of the species. The result of the stem volume analysis was compared with the existing stem volume model of Larix kaempferi species of South Korea which was developed by the Korea Forest Research Institute and with a simple volume model that was developed with fitting dataset in this study. The compatible model provided accurate prediction of the total stem volume when compared to the existing stem volume model and with a simple volume model. It is concluded that the compatible taper and stem volume equations are more convenient to use and therefore it is recommended to be applied in the Larix kaempferi species of South Korea.
基金financially supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF(RIF2014-06)the Forestry Industry Research special funds for Public Welfare Projects(201504104)
文摘Nucleotide diversity (pi) and linkage disequilibrium (LD) analysis based on SNP marker could provide a sound basis for choosing an association analysis method. Japanese larch (Larix kaempferi) is an important timber coniferous tree species for pulping and papermaking, but its high lignin content has significantly restricted it application potential. In this study, the LACCASE gene, that plays an important regulatory role for lignin biosynthesis, was selected as research target. The full-length cDNA and genomic sequences of the encoding LkLAC8 gene were isolated from the LACCASE expressed sequence tags of the Japanese larch transcriptome database using the rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). The cDNA was determined to be 1940 bp, with an open reading frame (ORF, 1734 bp) that encoded a protein of 577 AA. This protein contains four highly specific Cu2+ binding sites and 11 glycosylation sites, thus belonging to the LACCASE family. The deduced protein sequence shared an 89% identity with the PtaLAC from Pinus taeda. A real-time PCR analysis showed that the LkLAC8 transcript was expressed predominantly in mature xylem, with moderate levels in the immature xylem, cambium and mature leaves, the lowest in the roots. Lastly, the genomic sequences of LkLAC8 in 40 individuals from six naturally distributed populations of Japanese larch were amplified, and a total of 201 SNPs (103 and 98 mutation types of transition and transversion, respectively) were detected; the frequency of the SNPs was 1/19 bp. Nucleotide diversity among the six populations ranged from 0.0034 to 0.0053, which suggested that there were no significant differences among the populations. The LD analysis showed that the LD level decayed rapidly within the increasing length of the LkLAC8 gene. These results implied that LD mapping and association analysis based on candidate gene may be feasible for the marker-assisted breeding of new germplasms with low lignin in Japanese larch.
基金supported by Grants from the National Science and Technology Pillar Program of China(No.2015DAD09B01)
文摘Fifty-three larch interspecific hybrid clones(Larix kaempferi × L.gmelini) and their parent clones were used for growth curve analysis of height variations.The growth curves of the 55 clones were 'S'-shaped and 36 exhibited similar curves as the male parent.The coefficients of the logistic models were higher than 0.943,indicating that our results were effective in the simulation of the growth curves.ANOVA analysis showed significant differences in height of different clones (P/0.01).Average date of maximum height growth was Day 173,and average duration of rapid growth lasted for 50 days.Annual average increase in height was 9.7cm d^(-1) and daily average increase was 0.2 cm.The ratio of GR to the total annual increase in height ranged from 51.2 to 68.8%,with the average being 59.8%.There was a positive correlation between k values and plant heights which benefited from the evaluation of early plant height.There was also a positive correlation between GR(growth stage),GD(plant height) and annual increase in height.These results are informative to the evaluation of the elite clone selection and provide a theoretical basis for breeding and management.
文摘In this research, five plots are Selected based on stand storage and productivity of planted forest of Larix kaempferi (Japanese larch) as well as their relationship with factors suCh as forest age, site condition and stand density. Through standard plot investigation and method of trunk analysis, systematic research is conducted on storage and productivity of the planted forest and their relationship with site condition. As shown in the research, productivity of planted forest of Larix kaempferi in low latitude area is higher than that in high latitude area while stand productivity is higher in slight acid soil. Contents of potassium element in the soil have Considerable influence on productivity of the Larix kaempferi forest.
基金financially supported by Forest Science Technology Program from Korean National Institute of Forest Science
文摘Natural volatile organic compounds(VOCs) extracted from conifers such as P. koraiensis and L. kaempferi have long been studied for their anti-oxidant, anti-proliferative, and anti-inflammatory effects. To evaluate the anti-inflammatory effects of VOCs from P. koraiensis and L. kaempferi, lipopolysaccharide(LPS) was administered to generate a mouse model for inflammation by the nasal route to the lungs and intraperitoneally to the whole body. VOCs of P. koraiensis and L. kaempferi were exposed to the mice by standardized wood panels with closed system. Increased levels of serum IgE and PGE2 were observed after exposure to dexamethasone and VOCs. We further determined the expression levels of inflammatory cytokine mRNA in the LPS-induced inflammation model by the reverse transcription quantitative polymerase chain reaction. Furthermore, the levels of cyclooxygenase-2, tumor necrosis factor-α, interleukin-1β, and interleukin-13 were determined in peripheral blood mononuclear cells. Those inflammatory cytokines and the key enzyme for inflammation cyclooxygenase-2 expression in PBMCs were strongly reversed by dexamethasone and VOCs. Lung tissues after nasal LPS exposure showed increased cytokine mRNA expressions which were suppressed by treatment with dexamethasone and VOCs. Furthermore, the damage induced by LPS was attenuated by dexamethasone and VOCs. In conclusion, the results from the present study indicate that VOCs of P. koraiensis and L. kaempferi have a therapeutic potential in the treatment or prevention of local and systemic inflammation due to their immunosuppressive effects.
文摘The paper summarized the life cycle ,environmental features and distributions of Larix origination in Japan.The time of blooming and fruiting and the ways of cone collection and seeds storage were introduced.The treating methods of seeds germination and the sowing time for both Japanese larch (Larix kaempferi Carr)and Dahurian larch (Larix gmelinii var.Japonica Pilger) were discussed.