Effect of swimming training on neural microcirculation in rats with sciatic nerve compression A study based on laser Doppler flowmetry

Microcirculation of the peripheral nerve is necessary for neural growth and regeneration. However, technical limitations have limited studies in this area. The few studies conducted have concerned active exercise effects on microcirculation of the peripheral nerve. Using an animal experiment, this study evaluated the effect of swimming training on microcirculation of injured peripheral nerve by laser Doppler flowmetry. The results showed that the blood vessel at the distal end of the peripheral nerve was the main blood supply for the nerve, and the internal blood supply for the nerve had strong compensatory ability. Swimming training promoted the functional recovery of rats with sciatic nerve injury and the regeneration of myelin sheath and blood vessels, but had no impact on neural blood flow.

2-D Statistical Damage Detection of Concrete Structures Combining Smart Piezoelectric Materials and Scanning Laser Doppler Vibrometry

In the present study a new structural health monitoring (SHM) technique isproposed as well as a new damage index based on 2-D error statistics. The proposedtechnique combines the electromechanical impedance technique (EMI) which is based onthe use of piezoelectric Lead Zirconate Titanate (PZT) patches and Scanning LaserDoppler Vibrometry (SLDV) for damage detection purposes of concrete structures andearly age monitoring. Typically the EMI technique utilizes the direct and inversepiezoelectric effect of a PZT patch attached to a host structure via an impedance analyzerthat is used for both the actuation and sensing the response of the PZT-Host structuresystem. In the proposed technique the attached PZTs are actuated via a function generatorand the PZT-Host structure response is obtained by a Scanning Laser DopplerVibrometer. Spectrums of oscillation velocity of the surface of the attached PZTs verticalto the laser beam versus frequency are obtained and are evaluated for SHM purposes.This damage detection approach also includes the use of a damage index denoted asECAR (Ellipse to Circle Area Ratio) based on 2-D error statistics and is compared to theRoot Mean Square Deviation (RMSD) damage index commonly used in SHMapplications. Experimental results include ascending uniaxial compressive load ofconcrete cubic specimens, ascending three point bending of reinforced concrete beamspecimens and early age monitoring of concrete. Results illustrate the efficiency of theproposed technique in damage detection as well as early age monitoring as, in the firstcase, both severity and location of damage can be determined by examining the values ofdamage indices for each damaged state and in the early age monitoring case damageindices follow the strength gain curve.

Evaluation of microvascular reactivity with laser Doppler flowmetry in chronic kidney disease

Cardiovascular diseases are the major causes of mortality in patients with chronic kidney disease(CKD).The complex process of accelerated athero- and arteriosclerosis in CKD is associated with this phenomenon,where endothelial dysfunction(ED) is one of the initial steps. Hence, the early diagnosis of ED can potentially lead to early interventions which could result in a better outcome for these patients. Several methodologies have been developed for the diagnosis of ED. Laser Doppler flowmetry(LDF) enables us to study the microcirculation continuously in a non-invasive manner. In our review we would like to focus on different tests developed for LDF, like postocclusive reactive hyperaemia,local heating, iontophoresis, microdialysis or analysis of flowmotion. We would also like to summarize the available data in CKD with these methodologies to enlighten their perspectives in the clinical use on this patient population.

Investigation on Eccentric Agitation in the Stirred Vessel Using 3D-Laser Doppler Velocimeter

The spatial structure of the velocity field in a stirred vessel with water has been measured and analyzed using the laser Doppler velocimeter technique, with the immersing depth and agitation speed of the impeller re-maining approximately constant. The experimental results were provided such as the mean velocity field, fluctuat-ing velocities, turbulent kinetic energy, Reynolds shear stress and time series of the velocity in the stirred tank. These results probably provided the valuable basis to further optimize and enlarge the stirred tank in the industrial process.

Mucosal blood flow measurements using laser Doppler perfusion monitoring

Perfusion of individual tissues is a basic physiological process that is necessary to sustain oxygenation and nutrition at a cellular level. Ischemia, or the insuff iciency of perfusion, is a common mechanism for tissue death or degeneration, and at a lower threshold, a mechanism for the generation of sensory signalling including pain. It is of considerable interest to study perfusion of pe- ripheral abdominal tissues in a variety of circumstances. Microvascular disease of the abdominal organs has been implicated in the pathogenesis of a variety of disorders, including peptic ulcer disease, inflammatory bowel disease and chest pain. The basic principle of laser Doppler perfusion monitoring (LDPM) is to analyze changes in the spectrum of light reflected from tissues as a response to a beam of monochromatic laser light emitted. It reflects the total local microcirculatory blood perfusion, including perfusion in capillaries, arterioles, venules and shunts. During the last 20-25 years, numerous studies have been performed in different parts of the gastroin-testinal (GI) tract using LDPM. In recent years we have developed a multi-modal catheter device which includes a laser Doppler probe, with the intent primarily to investigate patients suffering from functional chest pain of presumed oesophageal origin. Preliminary studies show the feasibility of incorporating LDPM into such catheters for performing physiological studies in the GI tract. LDPM has emerged as a research and clinical tool in preference to other methods; but, it is important to be aware of its limitations and account for them when reporting results.

New Type of 2-D Laser Doppler Vibrometer

The fundamentals and method of 2 D laser Doppler vibrometer are introduced.The factors influencing the measuring accuracy are analyzed. Moreover, the circuit for signal processing is designed. The vibrating amplitude and frequency of 2 D vibration in wider range can be measured simultaneously in non contact means,the measuring results are accurate.

Water track laser Doppler velocimeter[Invited]

A water track laser Doppler velocimeter(LDV)is developed with advantages of high update rate,high real-time performance,high concealment,light weight,and small dimensions.The water track LDV measures the advance velocity of the underwater vehicle with respect to the surrounding water.The experimental results show that the water track LDV has an accuracy of 96.4%when the moving velocity of the vehicle with respect to the ground exceeds 0.25 m/s.Thus,the water track LDV is promising in the application of underwater navigation to aid the strapdown inertial navigation system.

Laser Doppler vibrometer for real-time speech-signal acquirement

Laser Doppler vibrometer （LDV） has a potential application prospect in remote sensing. Based on the correlation theories of heterodyne detection, a LDV system with a configuration of all fiber and heterodyne techniques is developed to detect the sound signal through the vibration of glass. Experimental results show that the LDV system has an ability to acquire the real-time speech signal 25 m away through glass. While, the system signal-to-noise ratio （SNR） value decreases with the increase of the glass thickness and the detection distance.

Extension of continuous scanning laser Doppler vibrometry measurement for complex structures with curved surfaces

The continuous Scanning Laser Doppler Vibrometry(SLDV)developed on the base of the galvanometer scanner system has made it possible to quickly obtain the full field vibration responses within a rectangular area of the structure.In this paper,an arbitrary continuous scanning path generating method for Continuous Scanning Laser Doppler Vibometry(CSLDV)is further put forward in order to allow the CSLDV suitable for testing structures featured by complex shapes not just for regular areas.In the first step,the relationship between position of laser spot and the driving voltages of galvanometer scanner system has been described by a mathematical modeling.Then,a novel arbitrary scanning path generating strategy based on CSLDV is presented by deforming a normalization rectangular scanning path to an arbitrary continuous scanning path.The mapping relation between the normalization rectangular scanning path and arbitrary continuous scanning path is established using the reference points.In the second step,a compressor blade with curved surface was taken as an example for modal test using the proposed method.At the same time,a validated experiment was performed in SLDV.The results show the mode shapes derived from the extended CSLDV are in agreement with those from SLDV and the Modal Assurance Criterion(MAC)between the two are all greater than 0.96.They also demonstrate the feasibility and effectiveness of the proposed method for CSLDV test and show strong potential on further practical engineering applications.

Design of new seismometer based on laser Doppler effect

In order to improve the resolution of seismic acquisition,a new seismic acquisition system based on tangential laser Doppler effect with an optimized differential optical configuration is proposed.The relative movement of the inertia object and the immobile frame is measured by laser Doppler effect,which can avoid the electromagnetic and thermometric interference,and the adoption of frequency-modulated (FM) transmission can improve the ability of anti-jamming.The frequency bandwidth is properly determined by analyzing the frequency of the Doppler signal.The velocity, displacement,acceleration, and frequency to be measured can be real-time acquired by frequency/velocity (F/V) converting the FM Doppler signal. A 100-dB dynamic range and the linear frequency range of 1.0 to 1000 Hz are realized.

Measurement of two-phase velocities in bubble flows using laser Doppler velocimetry

The measurement of two-phase velocities in bubble flows using laser Doppler velocimetry(LDV)is studied.The key to the problem is to differentiate the LDV signals from bubbles and tracers,based on which the two-phase velocities can be characterized.In this study,two experiments are carried out.Firstly,the bubble-chain experiment is performed to investigate the optical response of bubble surface and the corresponding LDV signal.The optical response shows that the light received by the LDV detector is dominated by the reflection component,which is similar to specular reflection to some extent.There are three typical patterns of signals of large bubbles passing through the measurement volume,all of which are with high amplitude and saturated.Then,the upward-flow experiment is conducted to study the statistical characteristics of large bubbles as well as micro tracers and micro bubbles.The results show that the amplitude of signal of millimeter bubbles is about an order of magnitude larger than that of tracers or micro bubbles.Based on this significant difference of the amplitude,we propose a phase discrimination method to distinguish two-phase signals.The capability of the proposed method is tested in a complex bubble flow,and its reliability is verified by bubble tracking velocimetry(BTV)technology.

Dual-polarization fiber grating laser-based laser Doppler velocimeter

A dual-frequency laser Doppler velocimeter implemented by a dual-polarization fiber grating laser is proposed, with the two laser frequencies produced by the two orthogonally polarized laser outputs of the fiber grating laser. The reflected laser outputs from a moving target experience the Doppler frequency shift, which is shown to be linearly related to the velocity and the beat note frequency difference between the laser outputs and the reflected light. With a digital frequency demodulation scheme, a high sensitivity of 0.64 MHz/（m/s） and a velocity resolution of less than 0.5% of the velocity for velocity measurement are demonstrated, which shows that the proposed laser Doppler velocimeter is capable of measurement of wide range of velocity.

Novel laser Doppler tachometer

In order to improve the accuracy and universality of the rotational speed measurement,an instantaneous rotational speed measurement method based on laser Doppler technology is proposed.The composition and working principle of the new system are discussed in detail.Theoretical and experimental results show that the new rotational speed measurement system belongs to the non-contact method,which eliminates the quantization error of the contact method and greatly improves the applicability and accuracy.Compared to the commonly used method,the measurement accuracy of the new rotational speed measurement system will not be affected by the system’s installation angle deviation,and it does not need to measure the radius of the rotating body,so the influence of the radius fluctuation of the rotating body on the measurement accuracy can be avoided while outputting the rotational speed in real time.The relative error of the rotational speed measurement is less than 0.06%(1σ).

The Influence of Age on Blood Flow and Temperature of Acupuncture Points: A Study based on Laser Doppler Flowmetry and Infrared Thermography

Background:To date,there has been a great lack of investigation on the influence of age on blood flow and temperature of acupoints in specific regions.Objective:This study aimed to determine the association between different age categories and acupoint blood flow/temperature on the forearm.Methods:Acupoint blood flow and temperature were measured in healthy adults of different age categories using laser doppler flowmetry(LDF)and infrared thermography(IRT),respectively.A total of 60 eligible healthy volunteers were divided into the young group,mid age group and old age group.All groups received LDF and IRT examination.Shenmen(HT7),Shaohai(HT3),Taiyuan(LU9)and Chize(LU5)of the Heart and Lung meridians on the forearm were selected as 4 test acupoints.Results:Regarding blood flow of the 4 test acupoints,the PU of Taiyuan(LU9)in the old age group was sig-nificantly different compared with that of the young age group(P<0.05)and the mid age group(P<0.05),while there was no significant difference in PU of the other acupoints between 3 groups(all P>0.05).Simi-larly,regarding acupoint temperature of the 4 test sites,the temperature of Shaohai(HT3)in the old age group was significantly different compared with that of the mid age group(P<0.05),while there was no significant difference in the temperature of the other 3 acupoints between 3 age groups(all P>0.05).Conclusion:Age category tends to have notable influence on the blood flow and temperature in specific acupoints in the forearm.Therefore,particular concerns should be taken into consideration regarding the effect of age differences for future studies in this field.Nevertheless,further studies with a large sample size and more test acupoints are needed to further verify current findings.

Laser Doppler flowmeter study on regional cerebral blood flow in early stage after standard superficial temporal artery-middle cerebral artery bypass surgery for moyamoya disease

Background Standard superficial temporal artery-middle cerebral artery （STA-MCA） bypass surgery is an effective treatment for moyamoya disease, but recent evidence suggests that postoperative cerebral hyperperfusion can occur. In this study, the trendline of changes in regional cerebral blood flow （rCBF） after surgery were continually monitored near the site of anastomosis in order to investigate both the efficacy of the procedure for improving rCBF and the possible risk of hyperperfusion . Methods Standard STA-MCA bypass surgery was performed on 13 patients, rCBF was measured continually using laser Doppler flowmetry （LDF） until the 5th day after the operation with the LDF probe implanted adjacent to the area of the anastomosis. The trendline of rCBF changes postoperatively was recorded for the analysis performed using SPSS 13.0. Results The baseline LDF value of cortical rCBF was （84.68±14.39） perfusion unit （PU）, which was linear relative to absolute perfusion volume before anastomosis and （88.90±11.26） PU immediately after anastomosis （P 〉0.05）. The value changed significantly from before to after anastomosis （P 〈0.05）; it was （417.72±21.35） PU on the 1st day after surgery, and （358.99±18.01） PU, （323.46±17.38） PU, （261.60±16.38） PU and （375.72±18.45） PU on the following 4 days, respectively. The rCBF decreased gradually from the 2nd day until the 4th postoperative day, but still was at a high level （P 〈0.05）. However, on the 5th postoperative day the rCBF increased again to the second highest level, which was significantly different compared with the baseline value （P 〈0.05）, but not significantly different compared with the values on the other 4 days （P〉0.05）. Conclusions STA-MCA anastomosis improves the cerebral blood supply significantly in the early stage after surgery, however, the risk of symptomatic hyperperfusion may exist, which may possibly occur on the 1st day and 5th day after surgery. A LDF is useful for postoperative monitoring for both the efficacy of bypass and possible risk of neurologic deterioration or bleeding from hyperperfusion.

Experimental study on minimum resolvable velocity for heterodyne laser Doppler vibrometry

A high spatial resolution, high velocity resolution all-fiber laser Doppler vibrometry （LDV） based on heterodyne detection for vibration measurements is reported. A linewidth of 1-kHz single-mode continuous fiber laser, polarization-preserving fiber, and a telescope with 30-ram aperture are used in this LDV. With the inphase-quadrature circuit and the digital differentiating discriminator, a high velocity resolution of 96.9 nm/s and a high displacement resolution of 2.5 pm are obtained simultaneously with a glass attached to a piezoceramic transducer. These values correspond to the measurement uncertainties of vibration velocity and displacement within 4.14% and 4.6%, respectively.

DETECTION ON MICRO-DOPPLER EFFECT BASED ON LASER COHERENT RADAR

A laser coherent detection system of 1550 nm wavelength was presented, and experimental research on detecting micro-Doppler effect in a dynamic target was developed. In the study, the return signal in the time domain is decomposed into a set of components in different wavelet scales by multi-resolution wavelet analysis, and the components are associated with the vibrational motions in a target. Then micro-Doppler signatures are extracted by applying the reconstruction. During the course of the final data processing frequency analysis and time-frequency analysis are applied to analyze the vibrational signals and estimate the motion parameters successfully. The experimental results indicate that the system can effectively detect micro-Doppler information in a moving target, and the tiny vibrational signatures also can be acquired effectively by wavelet multi-resolution analysis and time-frequency analysis.