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Three-dimensional image of hepatocellular carcinoma under confocal laser scanning microscope 被引量:7
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作者 Wang Hai Zhang Shi Neng Zhu +2 位作者 Shi Lun Lu Ya Lin Huang Peng Zhao 《World Journal of Gastroenterology》 SCIE CAS CSCD 2000年第3期344-347,共4页
AIM To investigate the application of confocallaser scanning microscopy(CLSM)in tumorpathology and three-dimensional( 3-D )reconstruction by CLSM in pathologic specimensof hepatocellular carcinoma(HCC).METHODS The 30... AIM To investigate the application of confocallaser scanning microscopy(CLSM)in tumorpathology and three-dimensional( 3-D )reconstruction by CLSM in pathologic specimensof hepatocellular carcinoma(HCC).METHODS The 30μm thick sections were cutfrom the paraffin-embedded tissues of HCC,hyperplasia and normal liver,stained with DNAfluorescent probe YOYO-1 iodide and examinedby CLSM to collect optical sections of nuclei and3-D images reconstructed.RESULTS HCC displayed chaotic arrangementof carcinoma cell nuclei,marked pleomorphism,indented and irregular nuclear surface,andirregular and coarse chromatin texture.CONCLUSION The serial optical tomograms ofCLSM can be used to create 3-D reconstruction ofcancer cell nuclei.Such 3-D impressions mightbe helpful or even essential in making anaccurate diagnosis. 展开更多
关键词 HCC NUCLEUS threedimensional reconstruction microscopy confocal laser scanning
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Visualization of Golgia apparatus as an intracellular calcium store by laser scanning confocal microscope 被引量:4
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作者 CUIJIE YANLI 《Cell Research》 SCIE CAS CSCD 1995年第2期165-179,共15页
Using laser scanning confocal microscopy, we have found that the in cells loaded with fluo-3/AM, highest intracellular Ca(2+) in the perinuclear region is associated with the Golgi apparatus. The spatiotemporal subcel... Using laser scanning confocal microscopy, we have found that the in cells loaded with fluo-3/AM, highest intracellular Ca(2+) in the perinuclear region is associated with the Golgi apparatus. The spatiotemporal subcellu lar distribution of Ca(2+) in living human fibroblasts exposing to calcium-free medium in response to agonists has been investigated. PDGF, which releases Ca(2+) from intracellular stores by inositol(1, 4, 5)-trisphosphate pathway,produced a biphasic transient rise in intracellular calcium.The initial rise was resulted from a direct release of calcium from the Golgi apparatus. Calcium could be also released from and reaccumulated into the Golgi apparatus by the stimulation of thapsigargin, an inhibitor of the Ca(2+) transport ATPase of intracellular calcium store. Permeablizing the plasma membrane by 10 μM digitonin resulted in the calcium release from the Golgi apparatus and depletion of the internal calcium store. These results suggest that the Golgi apparatus plays a role in Ca(2+) regulation in signal transduction. 展开更多
关键词 Golgi apparatus intracellular calcium store fluo-3/AM laser scanning confocal microscopy PDGF THAPSIGARGIN
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Revealing the F_actin Networks in Interphase Nuclei of Garlic Clove Cells by Confocal Fluorescence Microscopy 被引量:2
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作者 王冬梅 王学臣 张伟成 《Acta Botanica Sinica》 CSCD 2000年第11期1167-1171,共5页
The interphase nuclei of parenchyma cells and epidermal cells of garlic ( Allium sativum L.) clove were labelled with rabbit anti_actin antibody and FITC_conjugated goat anti_rabbit IgG antibody. The authors observ... The interphase nuclei of parenchyma cells and epidermal cells of garlic ( Allium sativum L.) clove were labelled with rabbit anti_actin antibody and FITC_conjugated goat anti_rabbit IgG antibody. The authors observed results with fluorescence microscopy and confocal laser scanning microscopy. The nuclei showed prominent green_yellow fluorescence, indicating the presence of actin in the nuclei. Fluorescence examination with TRITC_phalloidin showed distinctive red fluorescence in the nuclei, indicating that F_actin is present in the nuclei. Confocal laser scanning microscopy indicated the presence of F_actin containing network structures in the nuclei, but the network structures were absent and the nuclei still showed red fluorescence when the cells were treated with cytochalasin D before fixation; the red fluorescence in the nuclei was hard to be observed when the cells were treated with unlabelled phalloidin before the cells were stained with TRITC_phalloidin. These results indicate that F_actin is in the nuclei and forms network structures in the nuclei of garlic cells. 展开更多
关键词 interphase nucleus F_actin TRITC_phalloidin cytochalasin D confocal laser scanning microscopy Allium sativum
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Simultaneous multi-parameter observation of Harring-tonine-treating HL-60 cells with both two-photon and confocal laser scanning microscopy 被引量:7
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作者 张春阳 李艳平 +3 位作者 马辉 李素文 薛绍白 陈瓞延 《Science China(Life Sciences)》 SCIE CAS 2001年第4期383-391,共9页
Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb-Cephalotaxus hainanensis Li, can induce apoptosis in promyelocytic leukemia HL-60 cells. With both two-photon laser scanning microscopy and conf... Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb-Cephalotaxus hainanensis Li, can induce apoptosis in promyelocytic leukemia HL-60 cells. With both two-photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluores-cent probe Hoechst 33342, tetramethyrhodamine ethyl ester (TMRE) and Fluo 3-AM, we simulta-neously observed HT-induced changes in nuclear morphology, mitochondrial membrane potential and intracellular calcium concentration ([Ca2+]i) in HL-60 cells, and developed a real-time, sensitive and invasive method for simultaneous multi-parameter observation of drug- treating living cells at the level of single cell. 展开更多
关键词 TWO-PHOTON laser scanning microscopy confocal laser scanning microscopy Harringtonine HL-60 cells.
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Effect of melatonin on the spatial and temporal changes of [Ca^(2+) ]i in single living cells of cortical neurons by laser scanning confocal microscopy 被引量:5
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作者 张庆柱 张均田 《Chinese Medical Journal》 SCIE CAS CSCD 2000年第6期78-82,共5页
Objective To examine the effects of melatonin on the dynamic changes in the concentration of intracellular free Ca 2+ ([Ca 2+ ]i) in single intact cultured cortical neurons isolated from fetal rats, in order... Objective To examine the effects of melatonin on the dynamic changes in the concentration of intracellular free Ca 2+ ([Ca 2+ ]i) in single intact cultured cortical neurons isolated from fetal rats, in order to explore the possible antiaging mechanisms of melatonin (MT) Methods Using the highly fluorescent Ca 2+ sensitive indicator Fluo 3/AM, cortical neurons cultured in a 35?mm Tissue Culture Dish were in incubated for 45?min at room temperature with 5?μmol/L Fluo 3/AM, resulting in proper intracellular dye concentration to provide adequate signal strength for detection and excellent Laser Scanning Confocal Microscopy (LSCM) imaging of [Ca 2+ ]i while not disturbing normal intracellular physiology The changes in fluorescent intensity were monitored by LSCM Results Bay K8644 (10 6 ?mol/L), KCl (20 ?mmol/L), sodium L glutamate (Glu, 50?μmol/L) caused a rapid increase of [Ca 2+ ]i in cortical neurons, and this increase could be significantly attenuated by 10 6 and 10 7 mol/L MT Conclusions MT could antagonize the extracellular Ca 2+ influx, reduce Ca 2+ overload, and have a protective effect on neurons This may be one of the important antiaging mechanisms of MT 展开更多
关键词 MELATONIN CALCIUM laser scanning confocal microscopy Fluo-3/AM cerebral cortex NEURONS primary cell cultures AGING
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Single Particle-Based Confocal Laser Scanning Microscopy for Visual Detection of Copper Ions in Confined Space 被引量:1
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作者 Ke Wang Manping Qian +2 位作者 Honglan Qi Qiang Gao Chengxiao Zhang 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2021年第7期1804-1810,共7页
Main observation and conclusion A single particle-based confocal laser scanning microscopy was developed for the visual detection of copper ions in confined space.A fluorescence microparticle,named AuNCs/ZIF-8,was syn... Main observation and conclusion A single particle-based confocal laser scanning microscopy was developed for the visual detection of copper ions in confined space.A fluorescence microparticle,named AuNCs/ZIF-8,was synthesized by coating gold nanoclusters(AuNCs)onto the outer surface of zeolitic imidazolate framework-8(ZIF-8). 展开更多
关键词 Metal-organic frameworks FLUORESCENCE SENSORS Single particle confocal laser scanning microscopy
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Three-dimensional observation of the phase structure of high density polyethylene (HDPE)/poly(ethylene-co-butene) (PEB) blend by laser scanning confocal microscopy 被引量:1
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作者 ZHANG ChengGui DONG Xia +1 位作者 WANG DuJin HAN Charles C 《Chinese Science Bulletin》 SCIE EI CAS 2007年第15期2042-2047,共6页
In this paper,high density polyethylene (HDPE)/poly(ethylene-co-butene) (PEB) blend (50/50 wt%) was prepared through solution blending and then compression molding,and subsequently examined by laser scanning confocal ... In this paper,high density polyethylene (HDPE)/poly(ethylene-co-butene) (PEB) blend (50/50 wt%) was prepared through solution blending and then compression molding,and subsequently examined by laser scanning confocal microscopy (LSCM). The PEB used in this experiment was labeled with a small quantity of a fluorescein derivative to render fluorescence. The initial films showed uniform dye dis-tribution and no indication of phase separation within the resolution of optical microscopy. Sample films annealing at 140℃ followed by rapid cooling to room temperature showed obvious phase sepa-ration and bicontinuous structure. The present work indicates that by labeling one component with fluorescein derivative,LSCM can efficiently perform in situ depth profiling of polymer blends. 展开更多
关键词 激光扫描共焦显微镜 聚乙烯 形态学 互溶性 三维观测
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Dispersion of particles in the coatings characterized by laser scanning confocal micrscopy(LSCM) I:Vertical dispersion of particles in the coatings and the weathering property studied by orthogonal analysis method of LSCM
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作者 HAN Charles C. SUNG Li-Piin CLERICI Cyril 《Science China(Technological Sciences)》 SCIE EI CAS 2010年第8期2247-2251,共5页
Two kinds of TiO<sub>2</sub> filled epoxy coatings were designed and prepared to obtain pigments with different dispersion degrees of TiO<sub>2</sub> particles.Laser scanning confocal microscop... Two kinds of TiO<sub>2</sub> filled epoxy coatings were designed and prepared to obtain pigments with different dispersion degrees of TiO<sub>2</sub> particles.Laser scanning confocal microscope(LSCM)was used to investigate both the horizontal and vertical distributions of TiO<sub>2</sub> particles in the coatings.The results indicated that TiO<sub>2</sub> in the two samples shared considerable similarity in horizental dispersion,but exhibited great difference in vertical dispersion.TiO<sub>2</sub> showed uniform vertical distribution in disp coating,wheras a gap about 1.1μm was found in the non-disp coating,which significantly influenced the surface optical properties of the coatings during weathering.Based on the confocal data,the model of dispersion of pigments in the coatings was proposed and the change of surface properties during weathering was predicted:the surface optical properties showed an initial decrease followed by a subsequent increase,which was in good agreement with the weathering data. 展开更多
关键词 laser scanning confocal microscope(lscm) TiO 2 epoxy coatings VERTICAL DISPERSION of PARTICLES
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Evaluation of the intracellular trafficking of siRNAs in A375 cells by confocal laser scanning microscopy
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作者 Yiping Diao Jing Sun +3 位作者 Mengyi Yang Bo Xu Lihe Zhang Zhenjun Yang 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2016年第12期859-868,共10页
Investigation intracellular trafficking of siRNAs following their delivery to cells is of great interest to elucidate dynamics of siRNA in cytoplasm. In this study, we present a novel confocal laser scanning microsco... Investigation intracellular trafficking of siRNAs following their delivery to cells is of great interest to elucidate dynamics of siRNA in cytoplasm. In this study, we present a novel confocal laser scanning microscopy (CLSM) method to evaluate a novel delivery system of 3'-peptide-siRNA therapeutic, which was named 3'-pAs-siRNA/CLD. This method could not only calculate the content of the intracellular 3'-peptide-siRNA, but also quantify its co-localization with cellular substructure. We observed that 3'-pAs-siRNA/CLD, which provided the better antitumor capability, also had a better cell uptake, endosome escape and a longer retention time in A375. This novel strategy was proved to be efficient, quantified and visualized, thus making the dynamics research of siRNA in cytoplasm clear and simplified. 展开更多
关键词 siRNA/CLD complex Endosomal escape Cytoplasmic distribution confocal laser scanning microscopy
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Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution
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作者 Xin Xu Qin Luo +7 位作者 Jixiang Wang Yahui Song Hong Ye Xin Zhang Yi He Minxuan Sun Ruobing Zhang Guohua Shi 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2024年第6期41-56,共16页
Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously anal... Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample,such as different brain areas.In addition,conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm,a numerical aperture of 0.5,and a working wavelength range from 400 to 1000 nm.We achieved a resolution of 0.74μm in fluorescent beads imaging.The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system,a confocal laser scanning system,and a two-photon imaging system.This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution. 展开更多
关键词 mesoscopic objective lens large field-of-view high resolution MULTI-WAVELENGTH wide-field microscopy confocal laser scanning microscopy
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低氧缺血海马神经元内Ca^(2+)动态变化的LSCM测定 被引量:1
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作者 李峰 姚志彬 +1 位作者 谢瑶 陈以慈 《中国医学物理学杂志》 CSCD 2000年第1期41-43,共3页
测定脑组织细胞内 Ca^(2+)浓度是评价缺血引起神经元损伤的重要指标。本研究用激光扫描共聚焦显微镜(LSCM)和Fluo-3/AM荧光探针标记技术,从单个活细胞水平检测缺血刺激诱发的海马神经元内Ca2+瞬间动态变化。结果... 测定脑组织细胞内 Ca^(2+)浓度是评价缺血引起神经元损伤的重要指标。本研究用激光扫描共聚焦显微镜(LSCM)和Fluo-3/AM荧光探针标记技术,从单个活细胞水平检测缺血刺激诱发的海马神经元内Ca2+瞬间动态变化。结果显示低氧使胞内Ca2+浓度显著升高。谷氨酸引起Ca2+浓度升高缓慢但持续时间长。缺血对Ca2+浓度影响不显著。撤除葡萄糖则引起胞内Ca2+迅速降低。实验结果表明不同缺血刺激因素引起的钙振荡各异。用LSCM对活细胞内部非侵入光学断层扫描,能清晰记录到这些瞬态钙值变化。 展开更多
关键词 胞内钙离子 Fluo-3/AM 缺血 低氧 lscm 神经元
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应用LSCM对脑脊液淋巴瘤细胞内质网的研究
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作者 岳剑宁 孔凡元 +2 位作者 吴若芬 刘菊年 潘月英 《宁夏医学院学报》 2002年第1期1-3,9,T001,共5页
目的 :通过激光扫描共聚焦显微镜 (LSCM)技术对脑脊液淋巴瘤细胞内质网进行立体、动态、定量研究。方法 :将所观察脑脊液细胞分为正常淋巴细胞组、淋巴瘤细胞组 ,内质网以荧光探针DiOC6(3)标记后 ,得到内质网的断层扫描图像、荧光强度... 目的 :通过激光扫描共聚焦显微镜 (LSCM)技术对脑脊液淋巴瘤细胞内质网进行立体、动态、定量研究。方法 :将所观察脑脊液细胞分为正常淋巴细胞组、淋巴瘤细胞组 ,内质网以荧光探针DiOC6(3)标记后 ,得到内质网的断层扫描图像、荧光强度地形立体分布图并且定量测量内质网的荧光强度值。结果 :与正常淋巴细胞相比 ,淋巴瘤细胞内质网空间立体分布有明显差别 ,平均荧光值明显增强 ,有非常显著性差异 (P <0 0 1)。结论 :本研究对中枢神经系统淋巴瘤的诊断、鉴别诊断。 展开更多
关键词 激光扫描共聚焦显微镜 荧光探针 内质网 脑脊液 淋巴瘤细胞 lscm CSFC
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LSM 880 with Airyscan激光扫描共聚焦显微镜高级功能和管理 被引量:10
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作者 林曼娜 向承林 +2 位作者 李建军 徐江平 程玉芳 《电子显微学报》 CAS CSCD 北大核心 2019年第3期271-275,共5页
Zeiss LSM 880 with Airyscan是一款多功能、高分辨率、高效率的激光扫描共聚焦显微镜(以下称激光共聚焦显微镜)。结合使用和管理的经验,本文对激光共聚焦显微镜的原理、LSM 880 with Airyscan的高级功能及日常维护管理等内容进行详细... Zeiss LSM 880 with Airyscan是一款多功能、高分辨率、高效率的激光扫描共聚焦显微镜(以下称激光共聚焦显微镜)。结合使用和管理的经验,本文对激光共聚焦显微镜的原理、LSM 880 with Airyscan的高级功能及日常维护管理等内容进行详细介绍。列举了Airyscan、长时间多视野拍摄、Experiment Designer、荧光漂白后恢复(FRAP)、光谱扫描/拆分、荧光能量共振转移(FRET)、Tile Scan和Line Scan快速成像技巧等高级功能,供管理人员或同行使用者借鉴参考。 展开更多
关键词 激光共聚焦显微镜 LSM 880 with Airyscan 高级功能 管理
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Advances in Probing Wood-Coating Interface by Microscopy: A Review
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作者 Adya P. Singh Byung-Dae Park +1 位作者 Arif Nuryawan Menda Kazayawoko 《Journal of Surface Engineered Materials and Advanced Technology》 2013年第1期49-54,共6页
Surface coatings provide protection to wood products against weathering and other deteriorating factors, such as moisture uptake and microbial invasion. The effectiveness of coatings depends on many factors, including... Surface coatings provide protection to wood products against weathering and other deteriorating factors, such as moisture uptake and microbial invasion. The effectiveness of coatings depends on many factors, including how well the applied coatings adhere to the wood surface. Coating adhesion to wood involves both chemical and physical interactions between the coating and wood tissues in contact, and the particular focus of this mini-review will be on the advances being made in understanding the physical aspects of the interaction by probing wood-coating interface using novel and high resolution imaging techniques, including confocal laser scanning microscopy (CLSM), SEM-backscattered electron imaging and correlative microscopy employing light, confocal and scanning electron microscopy. 展开更多
关键词 Wood-Coating INTERFACE COATING Adhesion Correlative microscopy Backscattered Electron Imaging confocal laser scanning microscopy
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基于细胞微观形态定量的桃果实硬度变化差异性研究
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作者 白岚莎 吕健 +4 位作者 谢晋 王凤昭 吕明月 朱凤妹 毕金峰 《食品科学技术学报》 EI CAS CSCD 北大核心 2024年第5期115-124,共10页
为定量表征不同质地桃果实细胞微观形态及硬度变化的差异性,利用质构仪、多元显微成像结合计算机处理技术,追踪分析了贮藏期间不同质地桃果实(“美瑞”、“深州水蜜”及“金童5号”)果肉、果皮的硬度和细胞形态参数变化。结果表明,贮藏... 为定量表征不同质地桃果实细胞微观形态及硬度变化的差异性,利用质构仪、多元显微成像结合计算机处理技术,追踪分析了贮藏期间不同质地桃果实(“美瑞”、“深州水蜜”及“金童5号”)果肉、果皮的硬度和细胞形态参数变化。结果表明,贮藏期间桃果肉、果皮硬度均呈现显著下降趋势,其中果肉硬度降低幅度(58.68%~78.20%)显著大于果皮硬度降低幅度(35.53%~65.19%),更适用于桃硬度软化表征。贮藏初期(贮藏1 d),桃果肉细胞形态规则、排列紧密,其中“深州水蜜”细胞截面积(A)最大(1500~33000μm 2);“金童5号”果实细胞圆度为0.70~0.90的细胞占比约为92.80%。随贮藏时间延长,“深州水蜜”细胞融合现象加剧,出现了部分巨大细胞(A>35000μm 2);“美瑞”细胞截面孔隙率呈现持续增长趋势,细胞出现皱缩现象;“金童5号”细胞截面周长增幅最小,细胞形变幅度最低。扫描电镜和透射电镜结果进一步印证了,贮藏期间溶质桃“深州水蜜”细胞结构最为疏松,细胞壁解聚严重,细胞质溶出最为明显;不溶质桃“金童5号”细胞结构相对完整,细胞质少量溶出;硬质桃“美瑞”细胞由圆形转变为椭圆形,且其结构改变程度介于溶质桃与不溶质桃之间。研究基于细胞形态的定量表征,明确不同质地桃果实硬度的差异性改变,旨在为基于质地差异的桃果实分等分级和定量表征桃果实细胞形态与硬度改变提供理论依据。 展开更多
关键词 硬度 细胞形态 定量表征 激光共聚焦显微镜
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Dissolution behavior of Al_(2)O_(3)inclusions into CaO-MgO-SiO_(2)-Al_(2)O_(3)-TiO_(2)system ladle slags
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作者 Zhiyin Deng Xiaomeng Zhang +2 位作者 Guangyu Hao Chunxin Wei Miaoyong Zhu 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第5期977-987,共11页
To investigate the dissolution behaviors of Al_(2)O_(3)inclusions in CaO-5wt%MgO-SiO_(2)-30wt%Al_(2)O_(3)-TiO_(2)system ladle slags,confocal scanning laser microscopy was conducted on the slags with different TiO_(2)c... To investigate the dissolution behaviors of Al_(2)O_(3)inclusions in CaO-5wt%MgO-SiO_(2)-30wt%Al_(2)O_(3)-TiO_(2)system ladle slags,confocal scanning laser microscopy was conducted on the slags with different TiO_(2)contents(0-10wt%),and scanning electron microscopy was performed to study the interfacial reaction between Al_(2)O_(3)and this slag system.The results disclose that the dissolution of Al_(2)O_(3)inclusions does not result in the formation of new phases at the boundary between the slag and the inclusions.In TiO_(2)-bearing and TiO_(2)-free ladle slags,there is no difference in the dissolution mechanism of Al_(2)O_(3)inclusions at steelmaking temperatures.Boundary layer diffusion is found as the controlling step of the dissolution of Al_(2)O_(3),and the diffusion coefficient is in the range of 4.18×10^(-10)to 2.18×10^(-9)m^(2)/s at 1450-1500℃.Compared with the solubility of Al_(2)O_(3)in the slags,slag viscosity and temperature play a more profound role in the dissolution of Al_(2)O_(3)inclusions.A lower viscosity and a lower melting point of the slags are beneficial for the dissolution.Suitable addition of TiO_(2)(e.g.,5wt%)in ladle slags can enhance the dissolution of Al_(2)O_(3)inclusions because of the low viscosity and melting point of the slags,while excessive addition of TiO_(2)(e.g.,10wt%)shows the opposite trend. 展开更多
关键词 INCLUSIONS DISSOLUTION ladle refining slag titanium dioxide confocal scanning laser microscopy
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Changes in physicochemical characteristics of wheat flour and quality of fresh wet noodles induced by microwave treatment
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作者 Jian Zhang Xuejie Li +5 位作者 Xiujuan Ren Yanxia An Xiaoyan Song Yang Zhao Yaqing Wen Weifeng Zhang 《Grain & Oil Science and Technology》 CAS 2024年第3期177-185,共9页
Fresh wet noodles(FWN) are popular staple foods due to its unique chewy texture and favorable taste. However,the development of FWN is limited by its short shelf life and high browning rate. It has been found that the... Fresh wet noodles(FWN) are popular staple foods due to its unique chewy texture and favorable taste. However,the development of FWN is limited by its short shelf life and high browning rate. It has been found that the quantity of original microorganisms in wheat flour produced by traditional method is relatively high, which is detrimental to the processing quality and storage stability of FWN. Consequently, it becomes imperative to decrease microorganisms in wheat flour. Microwave treatment has been regarded as a promising method in the food industry due to its potential in inhibiting microbial growth and inactivating enzymes without causing adverse effect on the food quality. This study aims to investigate the effects of microwave treatment of wheat kernels under different powers(1, 2, 3, 4, 5 kW) on the physicochemical properties of wheat flour and the quality of FWN. The results revealed that microwave treatment had a significant effect on microbial inhibition and enzyme inactivation, wherein the total plate count(TPC) and yeast and mold counts(YMC) decreased by 0.87 lg(CFU/g) and 1.13 lg(CFU/g) respectively, and PPO activity decreased from 11.40 U to 6.31 U. The dough quality properties, such as stability, extensibility, and starch viscosity, improved significantly under different microwave conditions. Confocal laser scanning microscopy(CLSM) images indicated that starch and proteins aggregated gradually in treated flour, altering rheological properties of dough. From the results of scanning electron microscopy(SEM), microwave treatment led to the appearance of disrupted structure in the gluten proteins, but the secondary structure of proteins altered slightly. Rheological properties of dough confirmed that the microwave treatment greatly affected processing characteristics of wheat flour products, with significant advantageous consequences on product quality, especially for textural properties of FWN. Furthermore, FWN darkening could be inhibited noticeably after microwave treatment, thereby prolonging its shelf life. Therefore, microwave treatment could thus be an effective, practical technology to produce low-bacterial flour and thereby enhance its product quality. 展开更多
关键词 Microwave treatment STERILIZATION confocal laser scanning microscopy Rheological properties Protein structures
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Temperature dependence of intracellular free calcium in cardiac myocytes from rat and ground squirrel measured by confocal microscopy 被引量:2
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作者 王世强 周曾铨 钱洪 《Science China(Life Sciences)》 SCIE CAS 1999年第3期293-299,339-340,共9页
The temperature-dependence of infraeeliular free caleimn (Ca) was investigated in mdo-1 loaded ventricular myocytes from the ral, a non-hibernator, and from the ground squirrel, a hibernator. The dissociation constant... The temperature-dependence of infraeeliular free caleimn (Ca) was investigated in mdo-1 loaded ventricular myocytes from the ral, a non-hibernator, and from the ground squirrel, a hibernator. The dissociation constant of indo-l at different temperatures was calibrated both al pll-tat and at @-stat . and the result demonstrated that the @-stat ralibration should be prettrred . Analysis of the fluoreseent image showed a striking increase of Ca2 as well as spontaneous caleiuni waves in ral cells, indicating an overloaded cakuum. In contrast, cardiac myocytes of the ground sqnirraf were found to keep a constant (Ca2+) without caleium overload regardless of temperature variation. It is be-lieved that understanding of the mechanisms underlying the interccllular caleima homeostasis of hibrernators may lead to solutions of some medical questions . 展开更多
关键词 cardiac MYOCYTE CALCIUM HOMEOSTASIS HIBERNATION temperature confocal laser-scanning microscopy FLUORESCENT CALCIUM probe.
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Continuous live cell imaging of cellulose attachment by microbes under anaerobic and thermophilic conditions using confocal microscopy
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作者 Zhi-Wu Wang Seung-Hwan Lee +3 位作者 James G.Elkins Yongchao Li Scott Hamilton-Brehm Jennifer L.Morrell-Falvey 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2013年第5期849-856,共8页
Live cell imaging methods provide important insights into the dynamics of cellular processes that cannot be derived easily from population-averaged datasets.In the bioenergy field,much research is focused on fermentat... Live cell imaging methods provide important insights into the dynamics of cellular processes that cannot be derived easily from population-averaged datasets.In the bioenergy field,much research is focused on fermentation of cellulosic biomass by thermophilic microbes to produce biofuels;however,little effort is dedicated to the development of imaging tools to monitor this dynamic biological process.This is,in part,due to the experimental challenges of imaging cells under both anaerobic and thermophilic conditions.Here an imaging system is described that integrates confocal microscopy,a flow cell device,and a lipophilic dye to visualize cells.Solutions to technical obstacles regarding suitable fluorescent markers,photodamage during imaging,and maintenance of environmental conditions during imaging are presented.This system was utilized to observe cellulose colonization by Clostridium thermocellum under anaerobic conditions at 60℃.This method enables live cell imaging of bacterial growth under anaerobic and thermophilic conditions and should be widely applicable to visualizing different cell types or processes in real time. 展开更多
关键词 THERMOPHILE lipophilic dye CELLULOSE BIOFUEL confocal laser scanning microscopy biofilm
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同源四倍体水稻胚乳发育:极核融合和胚乳细胞化 被引量:22
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作者 王兰 刘向东 +3 位作者 卢永根 冯九焕 徐雪宾 徐是雄 《中国水稻科学》 CAS CSCD 北大核心 2004年第4期281-289,共9页
利用激光扫描共聚焦显微术和塑料包埋半薄切片技术观察同源四倍体水稻极核融合及游离核进一步发育的过程 ,发现同源四倍体水稻约有 1 /3子房极核融合过程和游离核进一步发育与其二倍体原种的基本一致 ;2 /3子房出现各种异常现象 ,如极... 利用激光扫描共聚焦显微术和塑料包埋半薄切片技术观察同源四倍体水稻极核融合及游离核进一步发育的过程 ,发现同源四倍体水稻约有 1 /3子房极核融合过程和游离核进一步发育与其二倍体原种的基本一致 ;2 /3子房出现各种异常现象 ,如极核未受精、受精异常和胚乳游离核发育滞后等。应用荧光增白剂染色对胚乳细胞化过程进行观察 ,直接观察到胚乳初生壁的出现和形成过程以及不同部位的胚乳细胞壁的形成机理。表明同源四倍体水稻与二倍体水稻胚乳的细胞化过程基本一致 ,胚乳细胞初始垂周壁来源于珠孔端胚囊壁内突向心游离生长 ,初始平周壁来源于游离核有丝分裂产生的成膜体 ,而中间胚乳细胞壁则有些来源于游离核有丝分裂产生的成膜体 ,有些是自由生长而来。同源四倍体水稻胚乳细胞化过程也出现相当比例的异常现象 ,如无细胞壁形成、细胞化不同步和产生分枝状的细胞壁等。同源四倍体水稻胚乳发育过程中出现的极核融合。 展开更多
关键词 同源四倍体 水稻 胚乳发育 极核融合 胚乳细胞化 激光扫描共聚焦显微术 荧光显微术
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