Two kinds of TiO<sub>2</sub> filled epoxy coatings were designed and prepared to obtain pigments with different dispersion degrees of TiO<sub>2</sub> particles.Laser scanning confocal microscop...Two kinds of TiO<sub>2</sub> filled epoxy coatings were designed and prepared to obtain pigments with different dispersion degrees of TiO<sub>2</sub> particles.Laser scanning confocal microscope（LSCM）was used to investigate both the horizontal and vertical distributions of TiO<sub>2</sub> particles in the coatings.The results indicated that TiO<sub>2</sub> in the two samples shared considerable similarity in horizental dispersion,but exhibited great difference in vertical dispersion.TiO<sub>2</sub> showed uniform vertical distribution in disp coating,wheras a gap about 1.1μm was found in the non-disp coating,which significantly influenced the surface optical properties of the coatings during weathering.Based on the confocal data,the model of dispersion of pigments in the coatings was proposed and the change of surface properties during weathering was predicted:the surface optical properties showed an initial decrease followed by a subsequent increase,which was in good agreement with the weathering data.展开更多
目的观察甘草黄酮对S180和H22荷瘤小鼠肿瘤细胞DNA及RNA的影响。方法S180和H22荷瘤小鼠随机分为甘草黄酮高、中、低剂量(25、11.25、5.58 m g/kg)组,阳性对照(环磷酰胺25 m g/kg)组和阴性对照(生理盐水)组,各组均sc给药。运用激光扫描...目的观察甘草黄酮对S180和H22荷瘤小鼠肿瘤细胞DNA及RNA的影响。方法S180和H22荷瘤小鼠随机分为甘草黄酮高、中、低剂量(25、11.25、5.58 m g/kg)组,阳性对照(环磷酰胺25 m g/kg)组和阴性对照(生理盐水)组,各组均sc给药。运用激光扫描共聚焦显微镜技术与吖啶橙(AO)荧光探针技术结合检测单一肿瘤细胞DNA和RNA的变化情况。结果不同剂量甘草黄酮组和环磷酰胺组的DNA及RNA荧光强度较生理盐水组有不同程度的减弱;甘草黄酮高、低剂量组RNA/DAN值与生理盐水组比较差异无显著性;甘草黄酮中剂量组、环磷酰胺组RNA/DNA值较生理盐水组有非常显著提高(P<0.01)。结论甘草黄酮与肿瘤细胞DNA和RNA结合后抑制其进一步复制与合成,可能是其抗肿瘤作用的途径之一。展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.20944004,50943026)the Open Research Program of the Key Laboratory of Rubber-Plastic(QUST),the Ministry of Education of China(Grant No.0805021)the PhD Research Program of QUST(Grant No.0022200)
文摘Two kinds of TiO<sub>2</sub> filled epoxy coatings were designed and prepared to obtain pigments with different dispersion degrees of TiO<sub>2</sub> particles.Laser scanning confocal microscope（LSCM）was used to investigate both the horizontal and vertical distributions of TiO<sub>2</sub> particles in the coatings.The results indicated that TiO<sub>2</sub> in the two samples shared considerable similarity in horizental dispersion,but exhibited great difference in vertical dispersion.TiO<sub>2</sub> showed uniform vertical distribution in disp coating,wheras a gap about 1.1μm was found in the non-disp coating,which significantly influenced the surface optical properties of the coatings during weathering.Based on the confocal data,the model of dispersion of pigments in the coatings was proposed and the change of surface properties during weathering was predicted:the surface optical properties showed an initial decrease followed by a subsequent increase,which was in good agreement with the weathering data.
文摘目的观察甘草黄酮对S180和H22荷瘤小鼠肿瘤细胞DNA及RNA的影响。方法S180和H22荷瘤小鼠随机分为甘草黄酮高、中、低剂量(25、11.25、5.58 m g/kg)组,阳性对照(环磷酰胺25 m g/kg)组和阴性对照(生理盐水)组,各组均sc给药。运用激光扫描共聚焦显微镜技术与吖啶橙(AO)荧光探针技术结合检测单一肿瘤细胞DNA和RNA的变化情况。结果不同剂量甘草黄酮组和环磷酰胺组的DNA及RNA荧光强度较生理盐水组有不同程度的减弱;甘草黄酮高、低剂量组RNA/DAN值与生理盐水组比较差异无显著性;甘草黄酮中剂量组、环磷酰胺组RNA/DNA值较生理盐水组有非常显著提高(P<0.01)。结论甘草黄酮与肿瘤细胞DNA和RNA结合后抑制其进一步复制与合成,可能是其抗肿瘤作用的途径之一。
