Matrix-assisted Laser Desorption/Ionization with Time-of-flight Mass Spectrometry (MALDI-TOFMS) was investigated as a method for the rapid identifica-tion of species. Current demand in microbial identi-fication is how...Matrix-assisted Laser Desorption/Ionization with Time-of-flight Mass Spectrometry (MALDI-TOFMS) was investigated as a method for the rapid identifica-tion of species. Current demand in microbial identi-fication is how to compare unknown strains to the known one quickly, semi-automatically and accurately. In this paper, we present a software tool that allows flexibly microbial matching in a user-friendly way, by letting the users to customize comparison parameters including: in vitro transcription enzyme, mass tolerance,minimum fragment length, intensity threshold and corresponding weights. We provide three spectral scoring functions to compute the affin-ity between the species. Therefore, the precision of microbial comparison increases. To test and verify this tool, we employed experimental spectral data based on MALDI-TOFMS and the gene sequences of E.coli and Salmonella. This software is written in Java for cross-platform intention.展开更多
激光时间传递技术是通过激光脉冲在空间的传播来实现地面与卫星时钟或地球上远距离两地时钟的同步,它具有很高的准确度和稳定度。一些国家已经成功进行了激光时间传递的试验,结果证明利用激光进行时钟之间的同步是有效可行的。介绍国内...激光时间传递技术是通过激光脉冲在空间的传播来实现地面与卫星时钟或地球上远距离两地时钟的同步,它具有很高的准确度和稳定度。一些国家已经成功进行了激光时间传递的试验,结果证明利用激光进行时钟之间的同步是有效可行的。介绍国内外已有的激光时间传递试验的情况和结果,重点介绍美国地面与机载原子钟之间的激光时间比对,以及法国的LASSO(LAser Syn-chronization from Stationary Orbit)和T2L2(Time Transfer by Laser Link)计划。展开更多
文摘Matrix-assisted Laser Desorption/Ionization with Time-of-flight Mass Spectrometry (MALDI-TOFMS) was investigated as a method for the rapid identifica-tion of species. Current demand in microbial identi-fication is how to compare unknown strains to the known one quickly, semi-automatically and accurately. In this paper, we present a software tool that allows flexibly microbial matching in a user-friendly way, by letting the users to customize comparison parameters including: in vitro transcription enzyme, mass tolerance,minimum fragment length, intensity threshold and corresponding weights. We provide three spectral scoring functions to compute the affin-ity between the species. Therefore, the precision of microbial comparison increases. To test and verify this tool, we employed experimental spectral data based on MALDI-TOFMS and the gene sequences of E.coli and Salmonella. This software is written in Java for cross-platform intention.
文摘激光时间传递技术是通过激光脉冲在空间的传播来实现地面与卫星时钟或地球上远距离两地时钟的同步,它具有很高的准确度和稳定度。一些国家已经成功进行了激光时间传递的试验,结果证明利用激光进行时钟之间的同步是有效可行的。介绍国内外已有的激光时间传递试验的情况和结果,重点介绍美国地面与机载原子钟之间的激光时间比对,以及法国的LASSO(LAser Syn-chronization from Stationary Orbit)和T2L2(Time Transfer by Laser Link)计划。
