Introduction: Cutaneous tuberculosis (CTB) is a rare form of extra-pulmonary tuberculosis that, when associated with late diagnosis, worsen the quality of life of the sick individuals. This report presents a case of l...Introduction: Cutaneous tuberculosis (CTB) is a rare form of extra-pulmonary tuberculosis that, when associated with late diagnosis, worsen the quality of life of the sick individuals. This report presents a case of late diagnosis of CTB. Unusual clinical manifestations retarded the correct tuberculosis diagnosis for more than a year. The immune response elicited by this type of tuberculosis as well as the factors that might contribute to the delay in diagnosis was evaluated and discussed. Methodology: Clinical evaluation and flow cytometric analyses of PBMC were realized for a case of CTB and a healthy individual as a control. Results: M. tuberculosis specific TCD8+ cell response was analyzed by flow cytometry and revealed positive cells for IL-17, IL-10, TGF-β and IDO. The CTB patient presented higher percentage of those cells when compared to a healthy donor. However, TCD8 cells positive for the important protective cytokine, IFN-γ was decreased in the CTB patient. Conclusion. The assessment of the M. tuberculosis specific TCD8+ immune response showed a regulatory/modulatory phenotype with a reduced IFN-γ response when compared to the healthy control that could have contributed to the CTB infection.展开更多
目的探讨妊娠中晚期乙型肝炎病毒(hepatits B virus,HBV)感染孕妇T细胞水平及对妊娠结局的影响。方法选取2020年5月—2021年3月于广州市花都区妇幼保健院就诊的HBV感染孕妇80例作为研究组。同时将研究组孕妇根据乙型肝炎E抗原(HBeAg)是...目的探讨妊娠中晚期乙型肝炎病毒(hepatits B virus,HBV)感染孕妇T细胞水平及对妊娠结局的影响。方法选取2020年5月—2021年3月于广州市花都区妇幼保健院就诊的HBV感染孕妇80例作为研究组。同时将研究组孕妇根据乙型肝炎E抗原(HBeAg)是否阳性分为两组,HBeAg(+)38例,HBeAg(-)42例。另外同期随机选取正常孕妇40名作为对照组。比较3组CD4^(+)、CD8^(+)及CD4^(+)/CD8^(+)比值水平;同时比较孕妇妊娠结局及与T细胞水平的相关性。结果研究组HBeAg(+)孕妇CD4^(+)及CD4^(+)/CD8^(+)比值显著低于HBeAg(-)孕妇及对照组,CD8^(+)水平显著高于HBeAg(-)孕妇及对照组,差异有统计学意义(P<0.001);HBeAg(+)孕妇剖宫产、胎膜早破、早产等不良妊娠结局显著高于HBeAg(-)孕妇及对照组,差异有统计学意义(P<0.05);HBeAg(+)孕妇中CD4^(+)及CD4^(+)/CD8^(+)比值与剖宫产、胎膜早破、早产呈负相关(r=-0.416、P=0.017;r=-0.508、P=0.012;r=-0.557、P=0.009;r=-0.436、P=0.015;r=-0.521、P=0.011;r=-0.596、P=0.008),CD8^(+)与剖宫产、胎膜早破、早产呈正相关(r=0.398、P=0.027;r=0.413、P=0.017;r=0.482、P=0.013)。结论妊娠中晚期HBV感染孕妇中,HBeAg(+)孕妇T细胞水平有明显变化,妊娠期应注意HBV感染孕妇HBeAg状态及T细胞水平变化,配合临床检测与适当治疗改善妊娠结局。展开更多
文摘Introduction: Cutaneous tuberculosis (CTB) is a rare form of extra-pulmonary tuberculosis that, when associated with late diagnosis, worsen the quality of life of the sick individuals. This report presents a case of late diagnosis of CTB. Unusual clinical manifestations retarded the correct tuberculosis diagnosis for more than a year. The immune response elicited by this type of tuberculosis as well as the factors that might contribute to the delay in diagnosis was evaluated and discussed. Methodology: Clinical evaluation and flow cytometric analyses of PBMC were realized for a case of CTB and a healthy individual as a control. Results: M. tuberculosis specific TCD8+ cell response was analyzed by flow cytometry and revealed positive cells for IL-17, IL-10, TGF-β and IDO. The CTB patient presented higher percentage of those cells when compared to a healthy donor. However, TCD8 cells positive for the important protective cytokine, IFN-γ was decreased in the CTB patient. Conclusion. The assessment of the M. tuberculosis specific TCD8+ immune response showed a regulatory/modulatory phenotype with a reduced IFN-γ response when compared to the healthy control that could have contributed to the CTB infection.
文摘目的探讨妊娠中晚期乙型肝炎病毒(hepatits B virus,HBV)感染孕妇T细胞水平及对妊娠结局的影响。方法选取2020年5月—2021年3月于广州市花都区妇幼保健院就诊的HBV感染孕妇80例作为研究组。同时将研究组孕妇根据乙型肝炎E抗原(HBeAg)是否阳性分为两组,HBeAg(+)38例,HBeAg(-)42例。另外同期随机选取正常孕妇40名作为对照组。比较3组CD4^(+)、CD8^(+)及CD4^(+)/CD8^(+)比值水平;同时比较孕妇妊娠结局及与T细胞水平的相关性。结果研究组HBeAg(+)孕妇CD4^(+)及CD4^(+)/CD8^(+)比值显著低于HBeAg(-)孕妇及对照组,CD8^(+)水平显著高于HBeAg(-)孕妇及对照组,差异有统计学意义(P<0.001);HBeAg(+)孕妇剖宫产、胎膜早破、早产等不良妊娠结局显著高于HBeAg(-)孕妇及对照组,差异有统计学意义(P<0.05);HBeAg(+)孕妇中CD4^(+)及CD4^(+)/CD8^(+)比值与剖宫产、胎膜早破、早产呈负相关(r=-0.416、P=0.017;r=-0.508、P=0.012;r=-0.557、P=0.009;r=-0.436、P=0.015;r=-0.521、P=0.011;r=-0.596、P=0.008),CD8^(+)与剖宫产、胎膜早破、早产呈正相关(r=0.398、P=0.027;r=0.413、P=0.017;r=0.482、P=0.013)。结论妊娠中晚期HBV感染孕妇中,HBeAg(+)孕妇T细胞水平有明显变化,妊娠期应注意HBV感染孕妇HBeAg状态及T细胞水平变化,配合临床检测与适当治疗改善妊娠结局。
