A homologue of the lower vertebrates translationally controlled tumor protein (TCTP) was cloned from the marine fish Japanese sea perch (Lateolabrax japonicus) by the technology of homology cloning. The full-length cD...A homologue of the lower vertebrates translationally controlled tumor protein (TCTP) was cloned from the marine fish Japanese sea perch (Lateolabrax japonicus) by the technology of homology cloning. The full-length cDNA sequence of the sea perch TCTP gene contained a 5' untranslated region (UTR) of 47 bp, a 3' UTR of 433 bp, and a putative open reading frame (ORF) of 510 bp encoding a polypeptide of 170 amino acids. The deduced amino acid sequence of the sea perch TCTP gene showed a high similarity to that of zebrafish, rohu, rabbit, chicken and human. Sequence analysis revealed there were a signature sequence of TCTP family, an N-glycosylation site, and five Casein kinase phosphorylation sites in the sea perch TCTP. The temporal expression of TCTP genes in healthy and lipopolysaccharide (LPS) challenged fishes was measured by semi-quantitative reverse transcription-PCR (RT-PCR). The results indicated that LPS could up-regulate the expression of sea perch TCTP in the examined tissues, including head-kidney, spleen and liver.展开更多
The Na^+/K^+/2Cl^-cotransporter(NKCC)and the cystic fibrosis transmembrane conductance regulator(CFTR)proteins play crucial roles in the transportation of Na^+and Cl^-.In this study,we identified cftr,nkcc1 a,nkcc1 b ...The Na^+/K^+/2Cl^-cotransporter(NKCC)and the cystic fibrosis transmembrane conductance regulator(CFTR)proteins play crucial roles in the transportation of Na^+and Cl^-.In this study,we identified cftr,nkcc1 a,nkcc1 b and nkcc2 in spotted sea bass(Lateolabrax maculatus)genomic and transcriptomic databases.We also characterized these genes via phylogenetic and structural analyses.The results showed that both cftr and nkcc were highly conservative in L.maculatus.Quantitative real-time polymerase chain reaction(qRT-PCR)analysis in ten tissues showed that cftr,nkcc1 a and nkcc2 highly express in osmoregulatory organs such as gill,kidney and intestine.Furthermore,the expressions of cftr and nkcc1 a in gill as well as nkcc2 in intestine were up-regulated by high salinity,indicating that these genes function potentially in osmoregulation.Our findings provided the insights into the cftr and nkcc functions in euryhaline teleost.展开更多
The metabolic rate of Japanese sea bass, Lateolabrax japonicus (C & V), was estimated in laboratory at temperature 25.2±0.5℃. The fresh weight of the fish was 4.64-52.28 g (average of 17.81±0.33 g). The...The metabolic rate of Japanese sea bass, Lateolabrax japonicus (C & V), was estimated in laboratory at temperature 25.2±0.5℃. The fresh weight of the fish was 4.64-52.28 g (average of 17.81±0.33 g). The routine metabolism was related to body weight by the exponential equation: R r =14.966 W 0.74 ( r =0.934). The rate of feeding metabolism increased linearly with food consumption. Feeding metabolic rate was 1.8-2.4 times the routine metabolic rate.展开更多
To investigate the effect of feed protein levels on growth and feed utilization of juvenile Lateolabraxjaponicus, fish meal was used as the major protein source to prepare five isoenergetic experimental feeds containi...To investigate the effect of feed protein levels on growth and feed utilization of juvenile Lateolabraxjaponicus, fish meal was used as the major protein source to prepare five isoenergetic experimental feeds containing 35.0%, 37.5%, 40.0%, 42.5% and 45.0% protein, respectively. Juvenile L. japonicus with the initial average body weight of （84.81 ±0.92） g were fed for 75 d to determine the best protein level for juvenile L. japonicus. The results showed that the relative weight gain rate, specific growth rate and feed conversion ratio in the group containing 39.85% protein were extremely higher than those in the groups containing 34.76% and 37.54% protein （ P 〈 0.01 ）, but had no significant difference with that in the group containing 42.34% protein （ P 〉0.05）. Protein efficiency ratio in the group containing 39.85% protein was also significantly higher than those in the groups containing 34.76%, 42.34% and 45.03% protein （ P 〈0.05）, while had no significant difference with that in the group containing 37.54% protein（ P 〉0.05）. In this experiment, based on quadratic model regression analysis of specific growth rate and protein efficiency ratio, growth performance and feed utilization rate of L. japonicus was the best when the protein content of feed was from 38.87% to 41.50%.展开更多
在半精制饲料中分别添加0、0.35%、0.70%、1.05%、1.40%、1.75%苏氨酸,制成苏氨酸实际梯度为1.05%、1.35%、1.65%、2.00%、2.42%、2.65%的6组等能等氮饲料(44.67%粗蛋白质,21.65 k J/g总能),对初始体重为(333.93±6.60)g的鲈鱼(Late...在半精制饲料中分别添加0、0.35%、0.70%、1.05%、1.40%、1.75%苏氨酸,制成苏氨酸实际梯度为1.05%、1.35%、1.65%、2.00%、2.42%、2.65%的6组等能等氮饲料(44.67%粗蛋白质,21.65 k J/g总能),对初始体重为(333.93±6.60)g的鲈鱼(Lateolabrax japonicus)在海水浮式网箱(1.5 m×1.5 m×2.0 m)中进行了70 d的喂养实验,研究其对苏氨酸的最适需求量。结果显示,鲈鱼成活率在89.58%–95.83%之间,各处理组之间无显著差异(P>0.05);随着饲料中苏氨酸水平的升高,鲈鱼的特定生长率(SGR)显著增加(P<0.05),且在2.00%苏氨酸饲料组出现最大值,但随着苏氨酸水平的继续升高,SGR呈减小的趋势;饲料效率(FE)随饲料中苏氨酸水平的升高呈先增加后减小的趋势,2.00%苏氨酸组的FE显著高于1.05%组及2.65%组(P<0.05);随着饲料中苏氨酸水平的升高,蛋白质沉积率(PPV)呈先增加后减小的趋势,且于2.00%苏氨酸组出现最大值;肝脏谷草转氨酶、谷丙转氨酶活性随饲料中苏氨酸水平的升高呈先增加后减小的趋势;饲料中不同水平苏氨酸对鱼体粗蛋白、粗脂肪、粗灰分无显著影响(P>0.05)。以特定生长率、饲料效率及蛋白质沉积率为评价指标,经二次回归分析得出,鲈鱼对饲料中苏氨酸的最适需求量分别为占饲料干重的1.84%、1.87%及1.83%,占饲料蛋白质的4.11%、4.18%及4.09%。展开更多
生长激素受体(GHR)作为GH/IGF轴的中心环节,在内分泌调控中发挥重要作用。本实验采用c DNA末端快速扩增法(RACE)技术克隆出花鲈GHR1和GHR2的c DNA全长序列。急性低盐度调控实验设为海水组、半海水组和淡水组。测定了急性低盐度调控24、4...生长激素受体(GHR)作为GH/IGF轴的中心环节,在内分泌调控中发挥重要作用。本实验采用c DNA末端快速扩增法(RACE)技术克隆出花鲈GHR1和GHR2的c DNA全长序列。急性低盐度调控实验设为海水组、半海水组和淡水组。测定了急性低盐度调控24、48、96、144、192h后,花鲈肝脏中GHRs、IGF-1及垂体GH表达情况。结果表明,GHR1 c DNA全长序列2436bp,编码637个氨基酸;GHR2 c DNA全长序列2940bp,编码582个氨基酸。GHR1与GHR2由信号肽、胞外区、跨膜区、胞内区组成,且结构存在差异。脑、肾、鳃中GHR1表达明显高于GHR2;而在肌肉、垂体、肝脏、盲肠、胸腺、心脏中,GHR2表达明显高于GHR1。24h时,各组GHR1表达不变,GHR2、GH、IGF-1显著下降。之后,相对于海水组,淡水组和半海水组GHRs和IGF-1表达升高,而GH下降,GH与GHR负相关。据结果推测,花鲈GHR2可能为SL受体,GH/IGF轴参与低渗调控可能是通过增加GHRs,进而激活下游IGF-1表达而实现。展开更多
基金supported by the“863"Prijetof China under contract Nos 2001AA628180 and 2002AA626020.
文摘A homologue of the lower vertebrates translationally controlled tumor protein (TCTP) was cloned from the marine fish Japanese sea perch (Lateolabrax japonicus) by the technology of homology cloning. The full-length cDNA sequence of the sea perch TCTP gene contained a 5' untranslated region (UTR) of 47 bp, a 3' UTR of 433 bp, and a putative open reading frame (ORF) of 510 bp encoding a polypeptide of 170 amino acids. The deduced amino acid sequence of the sea perch TCTP gene showed a high similarity to that of zebrafish, rohu, rabbit, chicken and human. Sequence analysis revealed there were a signature sequence of TCTP family, an N-glycosylation site, and five Casein kinase phosphorylation sites in the sea perch TCTP. The temporal expression of TCTP genes in healthy and lipopolysaccharide (LPS) challenged fishes was measured by semi-quantitative reverse transcription-PCR (RT-PCR). The results indicated that LPS could up-regulate the expression of sea perch TCTP in the examined tissues, including head-kidney, spleen and liver.
基金supported by the China Agriculture Research System (No. CARS-47)the National Natural Science Foundation of China (No. 31602147)
文摘The Na^+/K^+/2Cl^-cotransporter(NKCC)and the cystic fibrosis transmembrane conductance regulator(CFTR)proteins play crucial roles in the transportation of Na^+and Cl^-.In this study,we identified cftr,nkcc1 a,nkcc1 b and nkcc2 in spotted sea bass(Lateolabrax maculatus)genomic and transcriptomic databases.We also characterized these genes via phylogenetic and structural analyses.The results showed that both cftr and nkcc were highly conservative in L.maculatus.Quantitative real-time polymerase chain reaction(qRT-PCR)analysis in ten tissues showed that cftr,nkcc1 a and nkcc2 highly express in osmoregulatory organs such as gill,kidney and intestine.Furthermore,the expressions of cftr and nkcc1 a in gill as well as nkcc2 in intestine were up-regulated by high salinity,indicating that these genes function potentially in osmoregulation.Our findings provided the insights into the cftr and nkcc functions in euryhaline teleost.
文摘The metabolic rate of Japanese sea bass, Lateolabrax japonicus (C & V), was estimated in laboratory at temperature 25.2±0.5℃. The fresh weight of the fish was 4.64-52.28 g (average of 17.81±0.33 g). The routine metabolism was related to body weight by the exponential equation: R r =14.966 W 0.74 ( r =0.934). The rate of feeding metabolism increased linearly with food consumption. Feeding metabolic rate was 1.8-2.4 times the routine metabolic rate.
基金Supported by Science and Technology Spark Program of Fujian Province(2011S0015)
文摘To investigate the effect of feed protein levels on growth and feed utilization of juvenile Lateolabraxjaponicus, fish meal was used as the major protein source to prepare five isoenergetic experimental feeds containing 35.0%, 37.5%, 40.0%, 42.5% and 45.0% protein, respectively. Juvenile L. japonicus with the initial average body weight of （84.81 ±0.92） g were fed for 75 d to determine the best protein level for juvenile L. japonicus. The results showed that the relative weight gain rate, specific growth rate and feed conversion ratio in the group containing 39.85% protein were extremely higher than those in the groups containing 34.76% and 37.54% protein （ P 〈 0.01 ）, but had no significant difference with that in the group containing 42.34% protein （ P 〉0.05）. Protein efficiency ratio in the group containing 39.85% protein was also significantly higher than those in the groups containing 34.76%, 42.34% and 45.03% protein （ P 〈0.05）, while had no significant difference with that in the group containing 37.54% protein（ P 〉0.05）. In this experiment, based on quadratic model regression analysis of specific growth rate and protein efficiency ratio, growth performance and feed utilization rate of L. japonicus was the best when the protein content of feed was from 38.87% to 41.50%.
文摘生长激素受体(GHR)作为GH/IGF轴的中心环节,在内分泌调控中发挥重要作用。本实验采用c DNA末端快速扩增法(RACE)技术克隆出花鲈GHR1和GHR2的c DNA全长序列。急性低盐度调控实验设为海水组、半海水组和淡水组。测定了急性低盐度调控24、48、96、144、192h后,花鲈肝脏中GHRs、IGF-1及垂体GH表达情况。结果表明,GHR1 c DNA全长序列2436bp,编码637个氨基酸;GHR2 c DNA全长序列2940bp,编码582个氨基酸。GHR1与GHR2由信号肽、胞外区、跨膜区、胞内区组成,且结构存在差异。脑、肾、鳃中GHR1表达明显高于GHR2;而在肌肉、垂体、肝脏、盲肠、胸腺、心脏中,GHR2表达明显高于GHR1。24h时,各组GHR1表达不变,GHR2、GH、IGF-1显著下降。之后,相对于海水组,淡水组和半海水组GHRs和IGF-1表达升高,而GH下降,GH与GHR负相关。据结果推测,花鲈GHR2可能为SL受体,GH/IGF轴参与低渗调控可能是通过增加GHRs,进而激活下游IGF-1表达而实现。
