A new rearranged charnigrane sesquiterpene, named laurenokamurin, was isolated from the marine red alga Laurencia okamurai. Its structure was determined on the basis of spectroscopic methods.
To establish a micropropagation system of three Laurencia complex species （Laurencia okamurai, Laurencia tristicha, and Chondrophvcus undulatus） by tissue culture techniques, we studied the regeneration characterist...To establish a micropropagation system of three Laurencia complex species （Laurencia okamurai, Laurencia tristicha, and Chondrophvcus undulatus） by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20℃ and 35 （salinity）; for C. undulatus, 4 500 lx, 20℃ and 30; and for L. tristicha, 4 500 lx, 25℃ and 30.展开更多
基金supported by the National High-Tech R & D Project(No.2007AA09Z403)the National Natural Science Foundation of China(No.30530080)the Department of Science and Technology of Shandong Province(No.2006GG2205023).
文摘A new rearranged charnigrane sesquiterpene, named laurenokamurin, was isolated from the marine red alga Laurencia okamurai. Its structure was determined on the basis of spectroscopic methods.
基金This project was supported by the National Science & Technology Major Project (No. 2009ZX09301-001)Natural Science Foundation of China (Nos. 40976048,30730108 and 20721003)+1 种基金STCSM International Cooperation Project between SIMM/China and ICB/Italy (No. 10540702900)partly founded by the NSFC-TRF International Cooperation Project (No. 20911140471)~~
基金Supported by the fund from Jiangsu Key Laboratory of Marine Biotechnology (No. 2006HS006)Huaihai Institute of Technology, Lianyungang, China211-Project Funding of Soochow University (No. 14134908)
文摘To establish a micropropagation system of three Laurencia complex species （Laurencia okamurai, Laurencia tristicha, and Chondrophvcus undulatus） by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20℃ and 35 （salinity）; for C. undulatus, 4 500 lx, 20℃ and 30; and for L. tristicha, 4 500 lx, 25℃ and 30.
