Flavonoids identification, total flavonoids content and antioxidant activity of the extracts from the leaves as well as flowers of Cassia angustifolia were determined. Five different extraction techniques were used fo...Flavonoids identification, total flavonoids content and antioxidant activity of the extracts from the leaves as well as flowers of Cassia angustifolia were determined. Five different extraction techniques were used for the extraction of flavonoids from leaves and flowers. Total flavonoid contents (TFC) in the extracts were determined by UV-visible spectrophotometry. Individual flavonoids were identified and quantified by high-performance liquid chromatography coupled with photodiode array detection and electrospray ioniza- tion tandem mass spectrometry (HPLC-PDA-ESI-MS). It has been examined that aqueous ethanol (70%) fractions of C. angustifolia flowers and leaves are highly rich in flavonoids and microwave extraction is the best method for the extraction of individual flavanoid constituents (1 - 9) as well as total flavonoid contents. It was also found that compound 8 was absent in the leaves and compounds 2 and 5 could not be identified. The extracts of C. angustifolia flowers and leaves show strong antioxidant activity.展开更多
Near-infrared and mid-infrared spectroscopies were currently used to analyze natural compounds. During the last ten years various multiblocks methods were developed such as Concatenated PLS, Hierarchical-PLS (H-PLS), ...Near-infrared and mid-infrared spectroscopies were currently used to analyze natural compounds. During the last ten years various multiblocks methods were developed such as Concatenated PLS, Hierarchical-PLS (H-PLS), and MultiBlock-PLS (MB-PLS). These three algorithms were used to analyze 55 lavender (Lavandula angustifolia) essential oil samples. The results obtained were compared to the ones obtained respectively in NIR and MIR ranges. The accuracies of the models depend on the spectroscopic technique, pretreatment and the PLS methods. The results showed that the choice of the factor numbers used to build the multiblock models was the most important parameter for the H-PLS and MB-PLS methods.展开更多
An important marker in neurodegenerative Alzheimer’s disease (AD) is abnormal production of amyloid beta (Aβ) peptide leading to formation of plaques in the brain. Through decreasing Aβ aggregates, anti-inflammator...An important marker in neurodegenerative Alzheimer’s disease (AD) is abnormal production of amyloid beta (Aβ) peptide leading to formation of plaques in the brain. Through decreasing Aβ aggregates, anti-inflammatory agents, phagocytosis, and proteolytic enzymes are known to decline risk of Aβ plaque formation. In the previous study we showed that aqueous extract of Lavandula angustifolia (lavender), with known anti-inflammatory effects, improves memory deficits in animal model of Alzheimer. Here, we assess if lavender play a role in clearance of Aβ plaques in the hippocampus. The Alzheimeric animals were created with intracerebroventricular injection of Aβ 1-42. To confirm formation of Aβ plaques, brain sections were stained by Congo red method. Twenty days post-injection they were administered with different doses (50, 100 and 200 mg/kg) of the aqueous extract of lavender for duration of 20 days. Our results demonstrated that 50 mg/kg of lavender not effectively influenced the Aβ plaques. On the other hand, the herbal medicine at the doses of 100 and 200 mg/kg markedly decreased the extent of Aβ aggregates. We concluded that the lavender extract dose dependently underlies elimination of Aβ plaques. The exact mechanism by which the herbal medicine removes the Aβ aggregates needs to be elucidated.展开更多
The process of oxidation, due to free radicals, is the cause of major concern for human health. In particular damages related to the skin have great relevance;therefore, many antioxidants based products are developed ...The process of oxidation, due to free radicals, is the cause of major concern for human health. In particular damages related to the skin have great relevance;therefore, many antioxidants based products are developed and marketed with the intention to counteract the action of free radicals. The seed of Araucaria angustifolia is a rich source of antioxidants due to the presence of bioflavonoids to counteract free radicals damages. In this study, two extracts, one from the seed external teguments (shell) and the other from the inner seed pulp (endosperm and embryo) were obtained in order to evaluate possible applications to the dermo-cosmetic field. In parallel the following different methods were employed to characterize both the extracts and to determine their antioxidant capacity: HPTLC, ABTS and DPPH, ORAC and PLC. The qualitative analysis showed that both extracts have the antioxidant activity, but the quantitative evaluation revealed a more promising bioactivity from the shell than from the pulp. Therefore, it was evaluated the potential skin application of different cosmetic formulations, based on the presence of seed shell extract (W/O emulsion, O/W emulsion and gel). The best result was achieved with the W/O emulsion.展开更多
Food by-products containing bioactive substances, such as phenolic compounds, have garnered attention due to the possibility to increase the value of what would otherwise be considered residue. The present work sought...Food by-products containing bioactive substances, such as phenolic compounds, have garnered attention due to the possibility to increase the value of what would otherwise be considered residue. The present work sought to evaluate the extraction of phenolic compounds and their bioaccessibility from pinhão “comum” (Araucaria angustifolia var. angustifolia) and pinhão “macaco” (Araucaria angustifolia var. indehiscens) cooking water extracts during in vitro simulated gastrointestinal conditions. Our findings indicate that changes occurred depending on the type of extract and the gastrointestinal step. Although both of the evaluated pinhão extracts displayed bioaccessible phenolic compounds, the gradual bioaccessibility decrease of pinhão “macaco” extract during in vitro simulated gastrointestinal condition steps, characterizes this extract as the one with the best functional property. The functional property is related to antioxidant properties which are able to generate protective effects against various diseases.展开更多
Objective Alzheimer＇s disease（AD） is one of the most important neurodegenerative disorders.It is characterized by dementia including def icits in learning and memory.The present study aimed to evaluate the effects ...Objective Alzheimer＇s disease（AD） is one of the most important neurodegenerative disorders.It is characterized by dementia including def icits in learning and memory.The present study aimed to evaluate the effects of aqueous extract of lavender（Lavandula angustifolia） on spatial performance of AD rats.Methods Male Wistar rats were f irst divided into control and AD groups.Rat model of AD was established by intracerebroventricular injection of 10 ？g A？1-42 20 d prior to administration of the lavender extract.Rats in both groups were then introduced to 2 stages of task learning（with an interval of 20 d） in Morris water maze,each followed by one probe test.After the f irst stage of spatial learning,control and AD animals received different doses（50,100 and 200 mg/kg） of the lavender extract.Results In the first stage of experiment,the latency to locate the hidden platform in AD group was significantly higher than that in control group.However,in the second stage of experiment,control and AD rats that received distilled water（vehicle） showed similar performance,indicating that the maze navigation itself could improve the spatial learning of AD animals.Besides,in the second stage of experiment,control and AD rats that received lavender extract administration at different doses（50,100,and 200 mg/kg） spent less time locating the platform（except for the AD rats with 50 mg/kg extract treatment）,as compared with their counterparts with vehicle treatment,respectively.In addition,lavender extract significantly improved the performance of control and AD rats in the probe test,only at the dose of 200 mg/kg,as compared with their counterparts with vehicle treatment.Conclusion The lavender extract can effectively reverse spatial learning def icits in AD rats.展开更多
非特异性脂质转移蛋白(nsLTP,non-specific lipid transfer proteins)在植物脂质转运和分泌中发挥重要作用。本研究从薰衣草(Lavandula angustifolia)中克隆到2个II型nsLTP基因,命名为nsLTP2-1和nsLTP2-2,并对其进行功能分析。生信分析...非特异性脂质转移蛋白(nsLTP,non-specific lipid transfer proteins)在植物脂质转运和分泌中发挥重要作用。本研究从薰衣草(Lavandula angustifolia)中克隆到2个II型nsLTP基因,命名为nsLTP2-1和nsLTP2-2,并对其进行功能分析。生信分析表明,nsLTP2-1和ns LTP2-2分别编码119个和117个氨基酸,具有脂转移蛋白(LTP,lipid transfer proteins)保守结构域和8个高度保守的半胱氨酸残基;系统进化分析显示它们处于两个分支,与同科的紫苏(Perilla frutescens)相似性最高。基因表达分析显示2个基因均在花蕾中高表达,在叶片、茎和花瓣中几乎不表达,在花萼中的表达存在差异,nsLTP2-1和nsLTP2-2分别在成熟花萼和幼嫩花萼中表达量更高;2个基因在花蕾和叶片中的表达均受到强光诱导,且在花蕾中的表达均受脱落酸诱导,而叶片中nsLTP2-1和nsLTP2-2的表达分别受茉莉酸甲酯和乙烯诱导。亚细胞定位显示2个nsLTPs均定位在细胞膜和细胞壁上,可能与次生代谢物的转运有关。过表达nsLTP2-1和nsLTP2-2烟草叶片经尼罗红染色后,经485~543 nm激发光激发,叶片腺毛头部的荧光显示多于野生型,说明本研究中的nsLTPs可能在脂类的合成和转运中起重要作用。这些结果为明确薰衣草脂转移蛋白在脂类及萜类转运中的功能研究提供了参考。展开更多
文摘Flavonoids identification, total flavonoids content and antioxidant activity of the extracts from the leaves as well as flowers of Cassia angustifolia were determined. Five different extraction techniques were used for the extraction of flavonoids from leaves and flowers. Total flavonoid contents (TFC) in the extracts were determined by UV-visible spectrophotometry. Individual flavonoids were identified and quantified by high-performance liquid chromatography coupled with photodiode array detection and electrospray ioniza- tion tandem mass spectrometry (HPLC-PDA-ESI-MS). It has been examined that aqueous ethanol (70%) fractions of C. angustifolia flowers and leaves are highly rich in flavonoids and microwave extraction is the best method for the extraction of individual flavanoid constituents (1 - 9) as well as total flavonoid contents. It was also found that compound 8 was absent in the leaves and compounds 2 and 5 could not be identified. The extracts of C. angustifolia flowers and leaves show strong antioxidant activity.
文摘Near-infrared and mid-infrared spectroscopies were currently used to analyze natural compounds. During the last ten years various multiblocks methods were developed such as Concatenated PLS, Hierarchical-PLS (H-PLS), and MultiBlock-PLS (MB-PLS). These three algorithms were used to analyze 55 lavender (Lavandula angustifolia) essential oil samples. The results obtained were compared to the ones obtained respectively in NIR and MIR ranges. The accuracies of the models depend on the spectroscopic technique, pretreatment and the PLS methods. The results showed that the choice of the factor numbers used to build the multiblock models was the most important parameter for the H-PLS and MB-PLS methods.
文摘An important marker in neurodegenerative Alzheimer’s disease (AD) is abnormal production of amyloid beta (Aβ) peptide leading to formation of plaques in the brain. Through decreasing Aβ aggregates, anti-inflammatory agents, phagocytosis, and proteolytic enzymes are known to decline risk of Aβ plaque formation. In the previous study we showed that aqueous extract of Lavandula angustifolia (lavender), with known anti-inflammatory effects, improves memory deficits in animal model of Alzheimer. Here, we assess if lavender play a role in clearance of Aβ plaques in the hippocampus. The Alzheimeric animals were created with intracerebroventricular injection of Aβ 1-42. To confirm formation of Aβ plaques, brain sections were stained by Congo red method. Twenty days post-injection they were administered with different doses (50, 100 and 200 mg/kg) of the aqueous extract of lavender for duration of 20 days. Our results demonstrated that 50 mg/kg of lavender not effectively influenced the Aβ plaques. On the other hand, the herbal medicine at the doses of 100 and 200 mg/kg markedly decreased the extent of Aβ aggregates. We concluded that the lavender extract dose dependently underlies elimination of Aβ plaques. The exact mechanism by which the herbal medicine removes the Aβ aggregates needs to be elucidated.
文摘The process of oxidation, due to free radicals, is the cause of major concern for human health. In particular damages related to the skin have great relevance;therefore, many antioxidants based products are developed and marketed with the intention to counteract the action of free radicals. The seed of Araucaria angustifolia is a rich source of antioxidants due to the presence of bioflavonoids to counteract free radicals damages. In this study, two extracts, one from the seed external teguments (shell) and the other from the inner seed pulp (endosperm and embryo) were obtained in order to evaluate possible applications to the dermo-cosmetic field. In parallel the following different methods were employed to characterize both the extracts and to determine their antioxidant capacity: HPTLC, ABTS and DPPH, ORAC and PLC. The qualitative analysis showed that both extracts have the antioxidant activity, but the quantitative evaluation revealed a more promising bioactivity from the shell than from the pulp. Therefore, it was evaluated the potential skin application of different cosmetic formulations, based on the presence of seed shell extract (W/O emulsion, O/W emulsion and gel). The best result was achieved with the W/O emulsion.
文摘Food by-products containing bioactive substances, such as phenolic compounds, have garnered attention due to the possibility to increase the value of what would otherwise be considered residue. The present work sought to evaluate the extraction of phenolic compounds and their bioaccessibility from pinhão “comum” (Araucaria angustifolia var. angustifolia) and pinhão “macaco” (Araucaria angustifolia var. indehiscens) cooking water extracts during in vitro simulated gastrointestinal conditions. Our findings indicate that changes occurred depending on the type of extract and the gastrointestinal step. Although both of the evaluated pinhão extracts displayed bioaccessible phenolic compounds, the gradual bioaccessibility decrease of pinhão “macaco” extract during in vitro simulated gastrointestinal condition steps, characterizes this extract as the one with the best functional property. The functional property is related to antioxidant properties which are able to generate protective effects against various diseases.
基金supported by Deputy of Research,Kashan University of Medical Sciences,Kashan,I.R. Iran (No. 8835)
文摘Objective Alzheimer＇s disease（AD） is one of the most important neurodegenerative disorders.It is characterized by dementia including def icits in learning and memory.The present study aimed to evaluate the effects of aqueous extract of lavender（Lavandula angustifolia） on spatial performance of AD rats.Methods Male Wistar rats were f irst divided into control and AD groups.Rat model of AD was established by intracerebroventricular injection of 10 ？g A？1-42 20 d prior to administration of the lavender extract.Rats in both groups were then introduced to 2 stages of task learning（with an interval of 20 d） in Morris water maze,each followed by one probe test.After the f irst stage of spatial learning,control and AD animals received different doses（50,100 and 200 mg/kg） of the lavender extract.Results In the first stage of experiment,the latency to locate the hidden platform in AD group was significantly higher than that in control group.However,in the second stage of experiment,control and AD rats that received distilled water（vehicle） showed similar performance,indicating that the maze navigation itself could improve the spatial learning of AD animals.Besides,in the second stage of experiment,control and AD rats that received lavender extract administration at different doses（50,100,and 200 mg/kg） spent less time locating the platform（except for the AD rats with 50 mg/kg extract treatment）,as compared with their counterparts with vehicle treatment,respectively.In addition,lavender extract significantly improved the performance of control and AD rats in the probe test,only at the dose of 200 mg/kg,as compared with their counterparts with vehicle treatment.Conclusion The lavender extract can effectively reverse spatial learning def icits in AD rats.
文摘非特异性脂质转移蛋白(nsLTP,non-specific lipid transfer proteins)在植物脂质转运和分泌中发挥重要作用。本研究从薰衣草(Lavandula angustifolia)中克隆到2个II型nsLTP基因,命名为nsLTP2-1和nsLTP2-2,并对其进行功能分析。生信分析表明,nsLTP2-1和ns LTP2-2分别编码119个和117个氨基酸,具有脂转移蛋白(LTP,lipid transfer proteins)保守结构域和8个高度保守的半胱氨酸残基;系统进化分析显示它们处于两个分支,与同科的紫苏(Perilla frutescens)相似性最高。基因表达分析显示2个基因均在花蕾中高表达,在叶片、茎和花瓣中几乎不表达,在花萼中的表达存在差异,nsLTP2-1和nsLTP2-2分别在成熟花萼和幼嫩花萼中表达量更高;2个基因在花蕾和叶片中的表达均受到强光诱导,且在花蕾中的表达均受脱落酸诱导,而叶片中nsLTP2-1和nsLTP2-2的表达分别受茉莉酸甲酯和乙烯诱导。亚细胞定位显示2个nsLTPs均定位在细胞膜和细胞壁上,可能与次生代谢物的转运有关。过表达nsLTP2-1和nsLTP2-2烟草叶片经尼罗红染色后,经485~543 nm激发光激发,叶片腺毛头部的荧光显示多于野生型,说明本研究中的nsLTPs可能在脂类的合成和转运中起重要作用。这些结果为明确薰衣草脂转移蛋白在脂类及萜类转运中的功能研究提供了参考。
