Cadmium (Cd) Removal from Saline <i>Water by Veronica anagallis</i>and <i>Epilobium laxum</i>Plants in Hydroponic System

Present study was conducted to investigate the Cadmium (Cd) phytoextraction potential of two plants (Veronica anagallis-aquatic and Epilobium laxum Royle) for Cd removal from induced saline water. In hydroponic system, various concentrations of the Cd (50, 100, and 150 ppm) and NaCl salt (1000, 3000, and 6000 ppm) were used alone and in various combinations to evaluate the effect of salt (NaCl) concentrations on Cd absorption and accumulation in Veronica anagallis and Epilobium plants. The Cd at higher concentrations (100 and 150 ppm) significantly reduced the growth and biomass of both plants and addition of salt (NaCl) to growth media (Hoagland solution) further reduced the growth. The Cadmium (Cd) translocation factor (TF) of Epilobium plant was more than one (1), while the Veronica plant showed translocation factor less than 0.5. Veronica plant showed higher Bio-concentration factor (BCF) as more than 3.5 and Epilobium plant demonstrated Bio-concentration factor less than 1 (BCF 1 is a threshold limit for a plant to be hyper-accumulator of Cd). Conclusively, the Veronica anagallis plant is reported as Cd hyper-accumulator, while Epilobium laxum plant as non hyper-accumulator on the basis of BCF values in the present findings. Further study on Veronica and Epilobium plants is recommended.

松散棉(Gossypium laxum)核型的研究

用数量分析的方法对松散棉(G.laxum)的核型进行研究。结果表明,松散棉具一个中部着丝点染色体,10个近亚中部着丝点染色体,2个亚中部着丝点染色体。13对染色体中最长的为2.5微米,最短的为1.67微米,单倍染色体的长度总和为27.38微米。第4号和第6号染色体具随体。

Inhibitory Effect of Flavonoid Glycosides from Chlorophytum comosum on Nasopharyngeal Carcinoma 5-8F Cells and Its Mechanism

[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.

Structure and Activity of a New Sapogenin from Chlorophytum laxum R. Br.

A new steroidal sapogotin named 25-R-spirosta-3,5-dien-12β-ol（1） was isolated from the dried roots of Chlorophytum laxum R. Br. along with five known compounds, namely, diosgenin（2）, stigmasterol（3）, β-sitosterols（4）, estigmasterol-3-O-β-D-glicopyranoside（5） and 3-O-β-authemisol（6）. The structure of compound 1 was elucidated by the analysis of IR, HRESI-MS, 1D and 2D NMR spectral data. Compounds 2--5 were isolated from Chlorophytum laxum R. Br. In addition, all the compounds were evaluated for cytotoxicity on the human nasopharyngeal carcinoma cancer cell line 5-8F. Among them, the newly identified 25-R-spirosta-3,5-dien-12β-ol（1） and diosgenin（2） exhibited high cytotoxicity on 5-8F cells, with IC50 values of 24.8 and 41.9 μmol/L, respectively.

广西产四种绞股蓝及其混乱品种的生药学比较鉴定

对广西产的四种绞股蓝及其混乱品种的叶和茎进行了形态与组织比较,描述其鉴定特征,并制定了供鉴定用的检索表。

光叶绞股蓝营养成分的研究

本文分析了产于安徽岳西的光叶绞股蓝茎、叶中的蛋白质、氨基酸、总皂甙和矿质元素的含量．结果表明：光叶绞股蓝的茎、叶中均含有一定量的蛋白质，其中叶中的含量远高于茎，总皂甙的含量偏低，平均为１．６８％，且叶中含量高于茎；其叶、茎中均含有１７种氨基酸，包括有７种人体必需氨基酸和９种药效氨基酸，水解氨基酸中除蛋氨酸外，叶中的含量均高于茎；所测的４种大量矿质元素中，具有高Ｋ低Ｎａ的特点，叶和茎中还含有Ｚｎ、Ｃｕ、Ｆｅ、Ｍｎ、Ｓｒ等人体必需的微量有益元素．所以具有一定的开发利用价值．

广西五种绞股蓝属植物离体快繁与种质保存研究

以绞股蓝属植物的带芽茎段为材料,研究不同6-BA浓度与NAA 0.02mg·L-1组合对其诱导、分化和增殖的影响,并建立离体快繁体系。结果表明:MS+6-BA 2.0mg·L-1+NAA 0.02mg·L-1最适宜初代诱导,MS+6-BA 2.0mg·L-1+NAA 0.02mg·L-1最适合扁果绞股蓝的增殖培养,而MS+6-BA 1.5mg·L-1+NAA0.02mg·L-1是其它四种植物增殖的最佳培养基,在1/2MS+NAA 1.0mg·L-1上的生根率均达100%。1/2MS与蔗糖40g·L-1对五种植物的保存效果均最好;添加生长抑制剂能有效减缓生长速度,最佳生长抑制剂为ABA和CCC,浓度均为1.0mg·L-1,其中CCC能适合多个物种,连续保存360d的存活率均在94.5%以上;PP333不适合五种植物的保存。活力检测表明,各种质经保存后增殖、生根能力均未下降。

镉胁迫对三种绞股蓝属植物幼苗生长及光合特性的影响

目的:确定3种绞股蓝属植物对镉的耐受性,为阐释镉对绞股蓝属植物的毒害作用机理及下一步开展低镉积累种质筛选奠定基础。方法:以绞股蓝、缅甸绞股蓝和光叶绞股蓝为研究对象,用不同浓度氯化镉对3种植物的种子、萌发幼苗及水培苗进行处理。结果:镉处理对3种绞股蓝属植物种子的发芽率没有显著影响,但镉处理可以提高缅甸绞股蓝种子发芽势。低浓度镉处理可促进3种绞股蓝属植物萌发幼苗根的伸长,而高浓度镉处理则表现为抑制作用。在水培条件下,镉能影响3种绞股蓝属植物生长,导致植株矮小,生物量降低,并可导致叶片枯黄、萎缩。随着镉浓度增大,3种植物叶绿素含量逐渐降低,光合作用受到抑制。结论:3种绞股蓝属植物对镉均有一定的耐受性,其中,缅甸绞股蓝对镉的耐受性比其他2种植物相对更强。镉胁迫能降低3种绞股蓝属植物的叶绿素含量,使其光合作用受到限制,从而抑制植株生长。

三角草中三角草苷A的含量测定(英文)

目的建立三角草中三角草苷A的含量测定方法。方法高效液相色谱法测定三角草苷A的含量,采用Agilent TC-C18(Z)色谱柱(250 mm×4.6 mm,10μm),紫外检测波长为200 nm,以乙腈-1%磷酸溶液(24∶76)为流动相,流速为1.0 ml.min-1,柱温为25℃,进样量10μl,按外标法进行检测。结果供试品中三角草苷A得到较好的分离与测定,三角草苷A在0.48～4.8μg范围内线性关系良好,回归方程为:A=1E+06x-28 019,r2=0.999 9,平均回收率达99.02%,RSD=0.81%(n=6)。结论所建立的方法快速,简便,准确,重复性好,专属性好,可用于评价三角草的质量。

HPLC法同时测定三角草中黄酮类成分异荭草苷和异牡荆素的含量

目的建立同时测定三角草中异荭草苷和异牡荆素成分的高效液相色谱方法,为三角草质量评价提供参考。方法采用岛津高效液相色谱仪Shimadzu SPD-20AT,色谱柱:Agilent Ecilipse XDB-C_(18)柱(4.6 mm×250 mm,5.0μm);柱温为35℃,检测波长:339 nm,流动相为0.1%甲酸(A)-乙腈(B),梯度洗脱:7%~15%B(0~8 min),15%~20%B(8~12 min),20%~26%B(12~20 min),26%~35%(20~28 min),35%~50%B(28-35 min),50%~7%B(35~50 min),流速:1.0 mL·min^(-1)。结果异荭草苷和异牡荆素分别在0.049~0.738μg和0.044~0.660μg线性关系良好,加样回收了分别为100.70%和100.38%,两者的RSD分别为1.74%和1.83%。结论本研究建立的HPLC同时测定三角草中异荭草苷和异牡荆素成分含量方法,操作简单、结果准确、重复性好,可用于三角草的质量控制。

盈江危地马拉草评价及推广应用

盈江危地马拉草是云南省盈江县有较长栽培历史的粗糙型热带牧草地方品种。多年多点试验结果表明,该品种早期生长缓慢,刈后再生性较弱,营养生长期长且营养价值保持相对稳定;年平均干草产量为12.57～19.22 t/hm2,比对照德宏象草低29.02%～42.55%,但干季可利用干草产量比对照德宏象草高166.36%～242.03%。在饲草的可利用季节上盈江危地马拉草与德宏象草有较强的互补性,二者相互配合有利于实现云南南亚热带地区饲草的全年均衡供应,适合南亚热带冬季霜冻较轻的地区推广种植。

疏花长柄山蚂蝗复合体(豆科)的修订

通过文献、标本研究,结合野外考察及数据统计分析,确认疏花长柄山蚂蝗复合体包括4种1亚种,讨论了各分类群的重要性状,并给出了新的检索表。将湘西长柄山蚂蝗(Hylodesmum laxum subsp.falfolium)处理为原亚种的新异名;订正了《中国植物志》和《Flora of China》等著作中该类群的几个错误的地理分布,记载了我国几个省区的分布新记录,并给出了凭证标本。

小花吊兰染色体制片优化与核型分析

以小花吊兰根尖为试材,采用常规压片法制备染色体玻片标本,研究了取材时间、预处理方法、解离方法以及染色时间对小花吊兰根尖细胞染色体制片效果的影响,优化制片技术并进行核型分析,以期为小花吊兰建立更加稳定、明晰的品种鉴定方法。结果表明:于8:00取小花吊兰根尖,0.05%秋水仙素预处理3h,卡诺氏液固定,1.0mol/L盐酸60℃下解离15min,改良卡宝品红染色15min,所得小花吊兰染色体制片效果较好。对小花吊兰体细胞染色体数目进行统计,核型分析表明,细胞染色体数为2n=2x=16,中部着丝粒染色体(m)为3对,近中部着丝粒染色体(sm)为4对,近端部着丝粒染色体(st)1对,核型公式为2n=2x=16=6m+8sm+2st,核型属2A型,核型不对称系数为64.39%。