Mechanism of the Effect of Bacillus coagulans on Production Performance of Laying Hens in Late Laying Period

[Objective]The paper was to investigate the mechanism of Bacillus coagulans preparation in improving production performance of laying hens in late period of laying.[Method]A total of 648 individuals of"Jingfen 1"laying hens(66 weeks of age)in late period of laying were randomly divided into four groups,six replicates each group,and each group had 27 individuals of laying hens.The laying hens in control group were fed with basal diet,and those in experimental groupsⅠ,Ⅱ and Ⅲ were fed with the basal diets added with 3.33×10^6,1×10^7,3.33×10^7 CFU/g B.coagulans,respectively.The pretrial lasted one week,and the formal test lasted eight weeks.[Result]Compared with the control group,the laying rate in group Ⅰ increased significantly,and the feed-gain ratio in experimental group Ⅱ decreased noticeably while the spleen index increased remarkably;the alkaline phosphatase activities and blood calcium content in experimental groups Ⅰ,Ⅱ and Ⅲ increased significantly(P<0.05),while the triglyceride content decreased remarkably(P<0.05);the urea content in experimental groups Ⅰ and Ⅱ decreased obviously(P<0.05).Adding B.coagulans significantly increased the specific activity of amylase,lipase and protease in various intestinal mucosa of small intestine(P<0.05);adding B.coagulans significantly increased the content of follicle-stimulating hormone(FSH)and estradiol(E2),and significantly increased the mRNA expression of follicle stimulating hormone receptor gene(FSHR).[Conclusion]B.coagulans preparation could significantly improve the production performance of laying hens in late period of laying;appropriately enhance the immune capacity of laying hens;improve the serum biochemical indicators;increase the activity of digestive enzymes in the small intestine;and promote the release of gonadal hormone and the mRNA expression of FSHR gene in the ovary.

Effects of Xylooligosaccharide(XOS)and Probiotics(PRO)on Production Performance,Egg Quality and Intestinal Short-chain Fatty Acids of Laying Hens in Late Laying Period

[Objective]The paper was to study the effects of xylooligosaccharide(XOS)and probiotics(PRO)and their interaction on the production performance,egg quality and intestinal short-chain fatty acids of laying hens in late laying period.[Method]A total of 36074-week-old Romain brown laying hens were randomly divided into four groups,six replicates each group and 15 chickens each replicate.The trial lasted 63 d.The laying hens in control group(CON)were fed with basal diet,and those in experimental groups were fed with the basal diets adding with 150 mg/kg XOS(XOS group),100 mg/kg probiotics(PRO group),150 mg/kg XOS+100 mg/kg probiotics(XOS-PRO group).[Result]Compared with the control group,the laying rate,daily egg production,yolk ratio,colonic acetic acid and caecal propionic acid in XOS group increased significantly(P<0.05),and the feed gain ratio decreased remarkably(P<0.05).PRO had no significant effect on the production performance and egg quality of laying hens in late laying period(P>0.05),but significantly increased colonic acetic acid(P<0.05).XOS and PRO had no interaction effect on the production performance,egg quality and intestinal short-chain fatty acids of laying hens in late laying period(P<0.05).[Conclusion]Adding XOS to the diet of laying hens in late laying period could increase the content of short-chain fatty acids in the hindgut of laying hens,and effectively improve the production performance and egg quality of laying hens in late laying period.Adding PRO to the diet did not significantly improve the production performance and egg quality of laying hens,and there was no interaction effect between XOS and PRO.

辣诺提取物对产蛋高峰后期蛋鸡生产性能及蛋品质的影响

试验旨在研究辣诺提取物(辣木和诺丽果提取物)对产蛋高峰后期罗曼粉蛋鸡生产性能、蛋品质、血清抗氧化及生化指标的影响。选取产蛋率相近、健康的60周龄健康罗曼粉蛋鸡300只,随机分为2组,每组10个重复,每个重复15只。对照组饲喂基础饲粮,辣诺提取物组在基础饲粮中添加500 mg/kg辣诺提取物。预试期2周,正试期8周。结果表明:辣诺提取物组平均日采食量比对照组提高9.57%(P<0.05),平均蛋重有提高趋势(P=0.070);辣诺提取物组第2周蛋白高度有提高趋势(P=0.074),第6周和第8周蛋白高度分别比对照组高11.93%和12.12%(P<0.05),但鸡蛋储藏1周后辣诺提取物组鸡蛋蛋白高度和哈氏单位分别下降42.30%和29.67%(P>0.05);与对照组相比,辣诺提取物组第4周血清丙二醛含量有降低趋势(P=0.082),甘油三酯和低密度脂蛋白分别比对照组低28.00%和32.18%(P<0.05)。综上所述,产蛋高峰后期添加辣诺提取物不影响蛋鸡产蛋性能,但蛋品质和脂质代谢有一定程度改善。

产蛋高峰后期添加黑水虻虫干对蛋鸡生产性能、蛋品质、器官指数和血清指标的影响

试验旨在研究产蛋高峰后期添加黑水虻虫干对京粉1号蛋鸡生产性能、蛋品质、器官指数和血清指标的影响。选取45周龄生产性能相近、健康的京粉1号蛋鸡1152只,随机分为对照组、3%和6%黑水虻虫干组,每组6个重复,每个重复64羽蛋鸡。预试期1周,试验期8周。结果表明:与对照组相比,3%和6%黑水虻虫干组蛋鸡的正品蛋率和蛋黄颜色均有提高(P<0.05),次品蛋率降低(P<0.05),但3%和6%黑水虻虫干组间无显著差异;3%黑水虻虫干组的平均蛋重高于对照组和6%黑水虻虫干组(P<0.05);3%和6%黑水虻虫干组的肝脏指数均低于对照组(P<0.05),3%黑水虻虫干组的心脏指数和6%黑水虻虫干组的脾脏指数分别高于对照组(P<0.05);与对照组相比,3%和6%黑水虻虫干组的血清葡萄糖含量提高(P<0.05),6%黑水虻虫干组白蛋白和免疫球蛋白IgA含量增加(P<0.05),肌酸激酶和乳酸脱氢酶含量降低(P<0.05)。在本试验条件下,饲粮添加3%~6%黑水虻虫干提高了46~53周龄蛋鸡的生产性能和蛋黄颜色,提高了蛋鸡的免疫功能及机体健康状况。