YABBY genes are plant-specific transcription factors(TFs)that function in plant growth and development.We investigated the functions of the YABBY genes in plants’stress tolerance by analyzing the YABBY genes in foxta...YABBY genes are plant-specific transcription factors(TFs)that function in plant growth and development.We investigated the functions of the YABBY genes in plants’stress tolerance by analyzing the YABBY genes in foxtail millet(Setaria italica)and studying their functions on plant growth and responses to different stresses.Eight YABBY genes were identified on five chromosomes,which showed strong relationships with YABBY genes in other monocot species.Phylogenetical Si YABs were classified into four clades:FIL/YAB3,YAB2,INO,and CRC.No monocot YABBY member was classified into the YAB5 clade.Four conserved motifs were identified,and motif 1 constituted the YABBY domain,whereas motifs 2 and 3 formed the C2-C2 region.Si YAB genes were highly expressed in reproductive tissues.Si DL,one of the Si YABs,was selected to be overexpressed in Arabidopsis thaliana to check the functions of the YABBY genes.Overexpression of Si DL in A.thaliana caused delayed flowering,leaf curling,and reduced seed size.In addition,Si DL acted as a negative regulator in plant response to salt stress.Our study provides information to assist in studying the YABBY gene functions in S.italica.展开更多
Three field experiments were conducted during 2016 and 2017 in southwestern Ontario, Canada to evaluate the effect of glyphosate/dicamba (2:1 ratio) applied at the V2/V3 or V4/V5 growth stage on dicamba-resistant (DR)...Three field experiments were conducted during 2016 and 2017 in southwestern Ontario, Canada to evaluate the effect of glyphosate/dicamba (2:1 ratio) applied at the V2/V3 or V4/V5 growth stage on dicamba-resistant (DR) soybean injury, growth response and yield. At 1 DAA, glyphosate/dicamba at 450, 900, 1350, 1800 and 3600 g·ae·ha-1 caused 0, 0.3, 1.8, 3.3 and 5.8% growth response (leaf droop) when applied at the V2/V3 growth stage and 0.3%, 1.3%, 1.5%, 2.3% and 4.5% growth response when applied at V4/V5 growth stage in DR soybean, respectively. The growth response was similar at 3 DAA. This response was transient with no growth response observed at 7 DAA, 2 WAB, 4 WAB, 8 WAB, and late Sept. (R8 growth stage). Glyphosate/dicamba at 450, 900, 1350, 1800 and 3600 g·ae·ha-1 caused 0.0, 1.4%, 3.6%, 5.7% and 10.7% injury (speckled chlorosis and necrosis and leaf distortion) when applied at the V2/V3 growth stage and 0.5%, 0.7%, 2.0%, 3.7% and 6.9% injury when applied at the V4/V5 growth stage in DR soybean, respectively. The injury observed was transient with no injury observed at 4 and 8 WAB and late Sept. (R8). There was no impact of glyphosate/dicamba at various rates evaluated on maturity and seed yield except at 1350 g·ae·ha-1 (V2/V3) and 3600 g·ae·ha-1 (V4/V5) which caused a 6% reduction in yield of DR soybean yield compared to the weed-free control. Based on these results, glyphosate/dicamba at the labelled rates can be safely applied at the V2/V3 and V4/V5 growth stage in DR soybean. However, care is needed to avoid spray overlaps as the 2× rate can result in significant crop injury and yield reduction in DR soybean.展开更多
基金supported by grants from the National Key R&D Program of China(2021YFD1901101)the Key Science and Technology Program of Shanxi Province,China(20210140601026)the Project of Biological Breeding of Shanxi Agricultural University,China(YZGC102)。
文摘YABBY genes are plant-specific transcription factors(TFs)that function in plant growth and development.We investigated the functions of the YABBY genes in plants’stress tolerance by analyzing the YABBY genes in foxtail millet(Setaria italica)and studying their functions on plant growth and responses to different stresses.Eight YABBY genes were identified on five chromosomes,which showed strong relationships with YABBY genes in other monocot species.Phylogenetical Si YABs were classified into four clades:FIL/YAB3,YAB2,INO,and CRC.No monocot YABBY member was classified into the YAB5 clade.Four conserved motifs were identified,and motif 1 constituted the YABBY domain,whereas motifs 2 and 3 formed the C2-C2 region.Si YAB genes were highly expressed in reproductive tissues.Si DL,one of the Si YABs,was selected to be overexpressed in Arabidopsis thaliana to check the functions of the YABBY genes.Overexpression of Si DL in A.thaliana caused delayed flowering,leaf curling,and reduced seed size.In addition,Si DL acted as a negative regulator in plant response to salt stress.Our study provides information to assist in studying the YABBY gene functions in S.italica.
文摘Three field experiments were conducted during 2016 and 2017 in southwestern Ontario, Canada to evaluate the effect of glyphosate/dicamba (2:1 ratio) applied at the V2/V3 or V4/V5 growth stage on dicamba-resistant (DR) soybean injury, growth response and yield. At 1 DAA, glyphosate/dicamba at 450, 900, 1350, 1800 and 3600 g·ae·ha-1 caused 0, 0.3, 1.8, 3.3 and 5.8% growth response (leaf droop) when applied at the V2/V3 growth stage and 0.3%, 1.3%, 1.5%, 2.3% and 4.5% growth response when applied at V4/V5 growth stage in DR soybean, respectively. The growth response was similar at 3 DAA. This response was transient with no growth response observed at 7 DAA, 2 WAB, 4 WAB, 8 WAB, and late Sept. (R8 growth stage). Glyphosate/dicamba at 450, 900, 1350, 1800 and 3600 g·ae·ha-1 caused 0.0, 1.4%, 3.6%, 5.7% and 10.7% injury (speckled chlorosis and necrosis and leaf distortion) when applied at the V2/V3 growth stage and 0.5%, 0.7%, 2.0%, 3.7% and 6.9% injury when applied at the V4/V5 growth stage in DR soybean, respectively. The injury observed was transient with no injury observed at 4 and 8 WAB and late Sept. (R8). There was no impact of glyphosate/dicamba at various rates evaluated on maturity and seed yield except at 1350 g·ae·ha-1 (V2/V3) and 3600 g·ae·ha-1 (V4/V5) which caused a 6% reduction in yield of DR soybean yield compared to the weed-free control. Based on these results, glyphosate/dicamba at the labelled rates can be safely applied at the V2/V3 and V4/V5 growth stage in DR soybean. However, care is needed to avoid spray overlaps as the 2× rate can result in significant crop injury and yield reduction in DR soybean.
