A rumpled and twisted leaf 1(rtl1) mutant was generated from a japonica cultivar Nipponbare by ethyl methanesulfonate treatment,which was characterized as rumpled and twisted leaf at the seedling stage.The F2 populati...A rumpled and twisted leaf 1(rtl1) mutant was generated from a japonica cultivar Nipponbare by ethyl methanesulfonate treatment,which was characterized as rumpled and twisted leaf at the seedling stage.The F2 populations were constructed by crossing with indica cultivars TN1 and Zhefu 802,respectively.Genetic analysis demonstrated that the phenotype was controlled by a single recessive nuclear gene.The closely linked simple sequence repeat(SSR) marker RM1155 was obtained from bulked segregant analysis.Subsequently,sequence tagged site(STS) markers were developed using the published rice genome sequence.Finally,RTL1 was located between an STS marker T1591 and an SSR marker RM1359,at the distances of 0.48 cM and 0.96 cM,respectively.These results will facilitate the cloning of the target gene in further studies.展开更多
The yield potential of japonica/indica hybrids(JIH)has been achieved over 13.5 t ha–1 in large-scale rice fields,and some physiological traits for yield advantage of JIH over japonica inbred rice(JI)and indica hybrid...The yield potential of japonica/indica hybrids(JIH)has been achieved over 13.5 t ha–1 in large-scale rice fields,and some physiological traits for yield advantage of JIH over japonica inbred rice(JI)and indica hybrid rice(IH)were also identified.To date,little attention has been paid to morphological traits for yield advantage of JIH over JI and IH.For this reason,three JIH,three JI,and three IH were field-grown at East China(Ningbo,Zhejiang Province)in 2015 and 2016.Compared with JI and IH,JIH had 14.3 and 20.8%higher grain yield,respectively,attributed to its more spikelets per panicle and relatively high percentage of filled grains.The advantage in spikelets per panicle of JIH over JI and IH was shown in number of grains on the upper,middle,and lower branches.Compared with JI and IH,JIH had higher leaf area through leaf width and lower leaf angle of upper three leaves,higher leaf area index and leaf area per tiller at heading and maturity stages,higher stem weight per tiller and K and Si concentrations of stem at maturity,higher dry matter weight in leaf,stem,and panicle at heading and maturity stages,and higher biomass accumulation after heading and lower biomass translocation from stem during ripening.Leaf width of upper three leaves were correlated positively,while leaf angle of upper three leaves were correlated negatively with biomass accumulation after heading,stem weight per tiller,and per unit length.Our results indicated that the grain yield advantage of JIH was ascribed mainly to the more spikelets per panicle and relatively high percentage of filled grains.Higher leaf area through leaf width and more erect leaves were associated with improved biomass accumulation and stem weighing during ripening,and were the primary morphological traits underlying higher grain yield of JIH.展开更多
Three typical hybrid rice cultivars,together with three artificially modified plant types by the application of N fertilizer during the elongation of the two uppermost leaves were used to analyze how the plant type af...Three typical hybrid rice cultivars,together with three artificially modified plant types by the application of N fertilizer during the elongation of the two uppermost leaves were used to analyze how the plant type affected the layered leaf area and radiation transmission.Plant type factors,layered leaf area and radiation distribution were measured at the full heading,10 d and 25 d after full heading stages,respectively.A model for calculating the layered leaf area from plant type factors was established and validated to determine the effects of plant type factors on the layered leaf area for the three hybrids.Furthermore,the relationship between layered leaf area and radiation transmission was established by using the radiation transmission model.The effects of the plant type factors on the radiation transmission for the three hybrids were evaluated by using this model.Finally,a method was established to describe the canopy structure,such as the layered leaf area index and the radiation distribution in the rice canopy.展开更多
A leaf inclination angle distribution model, which is applicable to simulate leaf inclination angle distribution in six heights of layered canopy at different growth stages, was established by component factors affect...A leaf inclination angle distribution model, which is applicable to simulate leaf inclination angle distribution in six heights of layered canopy at different growth stages, was established by component factors affecting plant type in rice. The accuracy of the simulation results was validated by measured values from a field experiment. The coefficient of determination(R2) and the root mean square error(RMSE) between the simulated and measured values were 0.9472 and 3.93%, respectively. The simulation results showed that the distribution of leaf inclination angles differed among the three plant types. The leaf inclination angles were larger in the compact variety Liangyoupeijiu with erect leaves than in the loose variety Shanyou 63 with droopy leaves and the intermediate variety Liangyou Y06. The leaf inclination angles were distributed in the lower range in Shanyou 63, which matched up with field measurements. The distribution of leaf inclination angles in the same variety changed throughout the seven growth stages. The leaf inclination angles enlarged gradually from transplanting to booting. During the post-booting period, the leaf inclination angle increased in Shanyou 63 and Liangyou Y06, but changed little in Liangyoupeijiu. At every growth stage of each variety, canopy leaf inclination angle distribution on the six heights of canopy layers was variable. As canopy height increased, the layered leaf area index(LAI) decreased in all the three plant types. However, while the leaf inclination angles showed little change in Liangyoupeijiu, they became larger in Shanyou 63 but smaller in Liangyou Y06. The simulation results used in the constructed model were very similar to the actual measurement values. The model provides a method for estimating canopy leaf inclination angle distribution in rice production.展开更多
Layered leaf area index (LAIk) is one of the major determinants for rice canopy. The objective of this study is to attain rice LAI k using morphological traits especially leaf traits that affected plant type. A theore...Layered leaf area index (LAIk) is one of the major determinants for rice canopy. The objective of this study is to attain rice LAI k using morphological traits especially leaf traits that affected plant type. A theoretical model based on rice geometrical structure was established to describe LAI k of rice with leaf length (Li), width (Wi), angle (Ai), and space (Si), and plant pole height (H) at booting and heading stages. In correlation with traditional manual measurement, the model was performed by high R2-values (0.95-0.89, n=24) for four rice hybrids (Liangyoupeijiu, Liangyou E32, Liangyou Y06, and Shanyou 63) with various plant types and four densities (3 750, 2 812, 1 875, and 1 125 plants per 100 m2) of a particular hybrid (Liangyoupeijiu). The analysis of leaf length, width, angle, and space on LAI k for two hybrids (Liangyoupeijiu and Shanyou 63) showed that leaves length and space exhibited greater effects on the change of rice LAI k . The radiation intensity showed a significantly negative exponential relation to the accumulation of LAI k , which agreed to the coefficient of light extinction (K). Our results suggest that plant type regulates radiation distribution through changing LAI k . The present model would be helpful to acquire leaf distribution and judge canopy structure of rice field by computer system after a simple and less-invasive measurement of leaf length, width, angle (by photo), and space at field with non-dilapidation of plants.展开更多
基金supported by the National Major Special Program of Breeding of Transgenetic Organisms New Variety(Grant Nos.2009ZX08001-022B,2009ZX08009-125B)National Natural ScienceFoundation of China(Grant No.30970171)
文摘A rumpled and twisted leaf 1(rtl1) mutant was generated from a japonica cultivar Nipponbare by ethyl methanesulfonate treatment,which was characterized as rumpled and twisted leaf at the seedling stage.The F2 populations were constructed by crossing with indica cultivars TN1 and Zhefu 802,respectively.Genetic analysis demonstrated that the phenotype was controlled by a single recessive nuclear gene.The closely linked simple sequence repeat(SSR) marker RM1155 was obtained from bulked segregant analysis.Subsequently,sequence tagged site(STS) markers were developed using the published rice genome sequence.Finally,RTL1 was located between an STS marker T1591 and an SSR marker RM1359,at the distances of 0.48 cM and 0.96 cM,respectively.These results will facilitate the cloning of the target gene in further studies.
基金financed by the National Key Research and Development Program of China (2018YFD0300802)the Key Research and Development Program of Jiangsu Province, China (BE2016351)the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD)
文摘The yield potential of japonica/indica hybrids(JIH)has been achieved over 13.5 t ha–1 in large-scale rice fields,and some physiological traits for yield advantage of JIH over japonica inbred rice(JI)and indica hybrid rice(IH)were also identified.To date,little attention has been paid to morphological traits for yield advantage of JIH over JI and IH.For this reason,three JIH,three JI,and three IH were field-grown at East China(Ningbo,Zhejiang Province)in 2015 and 2016.Compared with JI and IH,JIH had 14.3 and 20.8%higher grain yield,respectively,attributed to its more spikelets per panicle and relatively high percentage of filled grains.The advantage in spikelets per panicle of JIH over JI and IH was shown in number of grains on the upper,middle,and lower branches.Compared with JI and IH,JIH had higher leaf area through leaf width and lower leaf angle of upper three leaves,higher leaf area index and leaf area per tiller at heading and maturity stages,higher stem weight per tiller and K and Si concentrations of stem at maturity,higher dry matter weight in leaf,stem,and panicle at heading and maturity stages,and higher biomass accumulation after heading and lower biomass translocation from stem during ripening.Leaf width of upper three leaves were correlated positively,while leaf angle of upper three leaves were correlated negatively with biomass accumulation after heading,stem weight per tiller,and per unit length.Our results indicated that the grain yield advantage of JIH was ascribed mainly to the more spikelets per panicle and relatively high percentage of filled grains.Higher leaf area through leaf width and more erect leaves were associated with improved biomass accumulation and stem weighing during ripening,and were the primary morphological traits underlying higher grain yield of JIH.
基金financially supported by the National Natural Science Foundation of China(Grant No.NSFC30871479)
文摘Three typical hybrid rice cultivars,together with three artificially modified plant types by the application of N fertilizer during the elongation of the two uppermost leaves were used to analyze how the plant type affected the layered leaf area and radiation transmission.Plant type factors,layered leaf area and radiation distribution were measured at the full heading,10 d and 25 d after full heading stages,respectively.A model for calculating the layered leaf area from plant type factors was established and validated to determine the effects of plant type factors on the layered leaf area for the three hybrids.Furthermore,the relationship between layered leaf area and radiation transmission was established by using the radiation transmission model.The effects of the plant type factors on the radiation transmission for the three hybrids were evaluated by using this model.Finally,a method was established to describe the canopy structure,such as the layered leaf area index and the radiation distribution in the rice canopy.
基金financially supported by the National Natural Science Foundation of China (Grant No. NSFC 30871479)
文摘A leaf inclination angle distribution model, which is applicable to simulate leaf inclination angle distribution in six heights of layered canopy at different growth stages, was established by component factors affecting plant type in rice. The accuracy of the simulation results was validated by measured values from a field experiment. The coefficient of determination(R2) and the root mean square error(RMSE) between the simulated and measured values were 0.9472 and 3.93%, respectively. The simulation results showed that the distribution of leaf inclination angles differed among the three plant types. The leaf inclination angles were larger in the compact variety Liangyoupeijiu with erect leaves than in the loose variety Shanyou 63 with droopy leaves and the intermediate variety Liangyou Y06. The leaf inclination angles were distributed in the lower range in Shanyou 63, which matched up with field measurements. The distribution of leaf inclination angles in the same variety changed throughout the seven growth stages. The leaf inclination angles enlarged gradually from transplanting to booting. During the post-booting period, the leaf inclination angle increased in Shanyou 63 and Liangyou Y06, but changed little in Liangyoupeijiu. At every growth stage of each variety, canopy leaf inclination angle distribution on the six heights of canopy layers was variable. As canopy height increased, the layered leaf area index(LAI) decreased in all the three plant types. However, while the leaf inclination angles showed little change in Liangyoupeijiu, they became larger in Shanyou 63 but smaller in Liangyou Y06. The simulation results used in the constructed model were very similar to the actual measurement values. The model provides a method for estimating canopy leaf inclination angle distribution in rice production.
基金supported by the National Natural Science Foundation of China (NSFC,30871479)
文摘Layered leaf area index (LAIk) is one of the major determinants for rice canopy. The objective of this study is to attain rice LAI k using morphological traits especially leaf traits that affected plant type. A theoretical model based on rice geometrical structure was established to describe LAI k of rice with leaf length (Li), width (Wi), angle (Ai), and space (Si), and plant pole height (H) at booting and heading stages. In correlation with traditional manual measurement, the model was performed by high R2-values (0.95-0.89, n=24) for four rice hybrids (Liangyoupeijiu, Liangyou E32, Liangyou Y06, and Shanyou 63) with various plant types and four densities (3 750, 2 812, 1 875, and 1 125 plants per 100 m2) of a particular hybrid (Liangyoupeijiu). The analysis of leaf length, width, angle, and space on LAI k for two hybrids (Liangyoupeijiu and Shanyou 63) showed that leaves length and space exhibited greater effects on the change of rice LAI k . The radiation intensity showed a significantly negative exponential relation to the accumulation of LAI k , which agreed to the coefficient of light extinction (K). Our results suggest that plant type regulates radiation distribution through changing LAI k . The present model would be helpful to acquire leaf distribution and judge canopy structure of rice field by computer system after a simple and less-invasive measurement of leaf length, width, angle (by photo), and space at field with non-dilapidation of plants.
