Western diet(rich in highly refined sugar and fat)can induce a range of metabolic dysfunctions in animals and humans,including neuroinflammation and cognitive function decline.Neuroinflammation and cognitive impairmen...Western diet(rich in highly refined sugar and fat)can induce a range of metabolic dysfunctions in animals and humans,including neuroinflammation and cognitive function decline.Neuroinflammation and cognitive impairment,two critical pathological characteristics of Alzheimer’s disease,have been closely associated with microbial alteration via the gut-brain axis.Thus,the present study aimed to investigate the influence of 2-O-β-D-glucopyranosyl-L-ascorbic acid(AA-2βG)isolated from the fruits of Lycium barbarum on preventing the high-fructose diet(HFrD)induced neuroinflammation in mice.It was found that AA-2βG prevented HFr D-induced cognitive deficits.AA-2βG also predominantly enhanced the gut barrier integrity,decreased lipopolysaccharide entry into the circulation,which subsequently countered the activation of glial cells and neuroinflammatory response.These beneficial effects were transmissible by horizontal fecal microbiome transplantation,transferring from AA-2βG fed mice to HFr D fed mice.Additionally,AA-2βG exerted neuroprotective effects involving the enrichment of Lactobacillus and Akkermansia,potentially beneficial intestinal bacteria.The present study provided the evidence that AA-2βG could improve indices of cognition and neuroinflammmation via modulating gut dybiosis and preventing leaky gut.As a potential functional food ingredient,AA-2βG may be applied to attenuate neuroinflammation associated with Western-style diets.展开更多
The communication system of high-speed trains in railway tunnels needs to be built with leaky cables fixed on the tunnel wall with special fixtures.To ensure safety,checking the regular leaky cable fixture is necessar...The communication system of high-speed trains in railway tunnels needs to be built with leaky cables fixed on the tunnel wall with special fixtures.To ensure safety,checking the regular leaky cable fixture is necessary to elimi-nate the potential danger.At present,the existing fixture detection algorithms are difficult to take into account detection accuracy and speed at the same time.The faulty fixture is also insufficient and difficult to obtain,seriously affecting the model detection effect.To solve these problems,an innovative detection method is proposed in this paper.Firstly,we presented the Res-Net and Wasserstein-Deep Convolution GAN(RW-DCGAN)to implement data augmentation,which can enable the faulty fixture to export more high-quality and irregular images.Secondly,we proposed the Ghost SENet-YOLOv5(GS-YOLOv5)to enhance the expression of fixture feature,and further improve the detection accuracy and speed.Finally,we adopted the model compression strategy to prune redundant channels,and visualized training details with Grad-CAM to verify the reliability of our model.Experimental results show that the algorithm model is 69.06%smaller than the original YOLOv5 model,with 70.07%fewer parameters,2.1%higher accuracy and 14.82 fps faster speed,meeting the needs of tunnel fixture detection.展开更多
BACKGROUND The gut–liver axis and bacterial translocation are important in cirrhosis,but there is no available universal biomarker of cellular bacterial translocation,for which presepsin may be a candidate.AIM To eva...BACKGROUND The gut–liver axis and bacterial translocation are important in cirrhosis,but there is no available universal biomarker of cellular bacterial translocation,for which presepsin may be a candidate.AIM To evaluate the relationship of the blood presepsin levels with the state of the gut microbiota in cirrhosis in the absence of obvious infection.METHODS This study included 48 patients with Child–Pugh cirrhosis classes B and C and 15 healthy controls.The fecal microbiome was assessed using 16S rRNA gene sequencing.Plasma levels of presepsin were measured.A total of 22 patients received a probiotic(Saccharomyces boulardii)for 3 months.RESULTS Presepsin levels were higher in patients with cirrhosis than in healthy individuals[342(91-2875)vs 120(102-141)pg/mL;P=0.048].Patients with elevated presepsin levels accounted for 56.3%of all included patients.They had lower levels of serum albumin and higher levels of serum total bilirubin and overall severity of cirrhosis as assessed using the Child–Pugh scale.Patients with elevated presepsin levels had an increased abundance of the main taxa responsible for bacterial translocation,namely Bacilli and Proteobacteria(including the main class Gammaproteobacteria and the minor taxa Xanthobacteraceae and Stenotrophomonas),and a low abundance of bacteria from the family Lachnospiraceae(including the minor genus Fusicatenibacter),which produce short-chain fatty acids that have a positive effect on intestinal barrier function.The presepsin level directly correlated with the relative abundance of Bacilli,Proteobacteria,and inversely correlated with the abundance of Lachnospiraceae and Propionibacteriaceae.After 3 months of taking the probiotic,the severity of cirrhosis on the Child–Pugh scale decreased significantly only in the group with elevated presepsin levels[from 9(8-11)to 7(6-9);P=0.004],while there were no significant changes in the group with normal presepsin levels[from 8(7-8)to 7(6-8);P=0.123].A high level of presepsin before the prescription of the probiotic was an independent predictor of a greater decrease in Child–Pugh scores(P=0.046),as well as a higher level of the Child–Pugh scale(P=0.042),but not the C-reactive protein level(P=0.679)according to multivariate linear regression analysis.CONCLUSION The level of presepsin directly correlates with the abundance in the gut microbiota of the main taxa that are substrates of bacterial translocation in cirrhosis.This biomarker,in the absence of obvious infection,seems important for assessing the state of the gut–liver axis in cirrhosis and deciding on therapy targeted at the gut microbiota in this disease.展开更多
A "leaky gut" may be the cutting edge for the passage of toxins, antigens or bacteria into the body, and may play a pathogenic role in advanced liver cirrhosis and its complications. Plasma endotoxin levels ...A "leaky gut" may be the cutting edge for the passage of toxins, antigens or bacteria into the body, and may play a pathogenic role in advanced liver cirrhosis and its complications. Plasma endotoxin levels have been admitted as a surrogate marker of bacterial translocation and close relations of endotoxemia to hyperdynamic circulation, portal hypertension, renal, cardiac, pulmonary and coagulation disturbances have been reported. Bacterial overgrowth, increased intestinal permeability, failure to inactivate endotoxin,activated innate immunity are all likely to play a role in the pathological states of bacterial translocation. Therapeutic approach by management of the gut-liver axis by antibiotics, probiotics, synbiotics, prebiotics and their combinations may improve the clinical course of cirrhotic patients. Special concern should be paid on anti-endotoxin treatment. Adequate management of the gut-liver axis may be effective for prevention of liver cirrhosis itself by inhibiting the progression of fibrosis.展开更多
Gut flora and bacterial translocation (BT) play important roles in the pathogenesis of chronic liver disease, including cirrhosis and its complications. Intestinal bacterial overgrowth and increased bacterial transloc...Gut flora and bacterial translocation (BT) play important roles in the pathogenesis of chronic liver disease, including cirrhosis and its complications. Intestinal bacterial overgrowth and increased bacterial translocation of gut flora from the intestinal lumen predispose patients to bacterial infections, major complications and also play a role in the pathogenesis of chronic liver disorders. Levels of bacterial lipopolysaccharide, a component of gram-negative bacteria, are increased in the portal and/or systemic circulation in several types of chronic liver disease. Impaired gut epithelial integrity due to alterations in tight junction proteins may be the pathological mechanism underlying bacterial translocation. Preclinical and clinical studies over the last decade have suggested a role for BT in the pathogenesis of nonalcoholic steatohepatitis (NASH). Bacterial overgrowth, immune dysfunction, alteration of the luminal factors, and altered intestinal permeability are all involved in the pathogenesis of NASH and its complications. A better understanding of the cell-specific recognition and intracellular signaling events involved in sensing gut-derived microbes will help in the development of means to achieve an optimal balance in the gut-liver axis and ameliorate liver diseases. These may suggest new targets for potential therapeutic interventions for the treatment of NASH. Here, we review some of the mechanisms connecting BT and NASH and potential therapeutic developments.展开更多
基金the financial support from the Key Research and Development Program of Ningxia Hui Autonomous Region of China(2021BEF02008)the National Natural Science Foundation of China(32272330)the Priority Academic Program Development of Jiangsu Higher Education Institutions。
文摘Western diet(rich in highly refined sugar and fat)can induce a range of metabolic dysfunctions in animals and humans,including neuroinflammation and cognitive function decline.Neuroinflammation and cognitive impairment,two critical pathological characteristics of Alzheimer’s disease,have been closely associated with microbial alteration via the gut-brain axis.Thus,the present study aimed to investigate the influence of 2-O-β-D-glucopyranosyl-L-ascorbic acid(AA-2βG)isolated from the fruits of Lycium barbarum on preventing the high-fructose diet(HFrD)induced neuroinflammation in mice.It was found that AA-2βG prevented HFr D-induced cognitive deficits.AA-2βG also predominantly enhanced the gut barrier integrity,decreased lipopolysaccharide entry into the circulation,which subsequently countered the activation of glial cells and neuroinflammatory response.These beneficial effects were transmissible by horizontal fecal microbiome transplantation,transferring from AA-2βG fed mice to HFr D fed mice.Additionally,AA-2βG exerted neuroprotective effects involving the enrichment of Lactobacillus and Akkermansia,potentially beneficial intestinal bacteria.The present study provided the evidence that AA-2βG could improve indices of cognition and neuroinflammmation via modulating gut dybiosis and preventing leaky gut.As a potential functional food ingredient,AA-2βG may be applied to attenuate neuroinflammation associated with Western-style diets.
基金supported by the National Natural Science Foundation of China(No.61702347,No.62027801)Natural Science Foundation of Hebei Province(No.F2022210007,No.F2017210161)+2 种基金Science and Technology Project of Hebei Education Department(No.ZD2022100,No.QN2017132)Central Guidance on Local Science and Technology Development Fund(No.226Z0501G)National innovation and Entrepreneurship training program for college students(No.202110107024).
文摘The communication system of high-speed trains in railway tunnels needs to be built with leaky cables fixed on the tunnel wall with special fixtures.To ensure safety,checking the regular leaky cable fixture is necessary to elimi-nate the potential danger.At present,the existing fixture detection algorithms are difficult to take into account detection accuracy and speed at the same time.The faulty fixture is also insufficient and difficult to obtain,seriously affecting the model detection effect.To solve these problems,an innovative detection method is proposed in this paper.Firstly,we presented the Res-Net and Wasserstein-Deep Convolution GAN(RW-DCGAN)to implement data augmentation,which can enable the faulty fixture to export more high-quality and irregular images.Secondly,we proposed the Ghost SENet-YOLOv5(GS-YOLOv5)to enhance the expression of fixture feature,and further improve the detection accuracy and speed.Finally,we adopted the model compression strategy to prune redundant channels,and visualized training details with Grad-CAM to verify the reliability of our model.Experimental results show that the algorithm model is 69.06%smaller than the original YOLOv5 model,with 70.07%fewer parameters,2.1%higher accuracy and 14.82 fps faster speed,meeting the needs of tunnel fixture detection.
文摘BACKGROUND The gut–liver axis and bacterial translocation are important in cirrhosis,but there is no available universal biomarker of cellular bacterial translocation,for which presepsin may be a candidate.AIM To evaluate the relationship of the blood presepsin levels with the state of the gut microbiota in cirrhosis in the absence of obvious infection.METHODS This study included 48 patients with Child–Pugh cirrhosis classes B and C and 15 healthy controls.The fecal microbiome was assessed using 16S rRNA gene sequencing.Plasma levels of presepsin were measured.A total of 22 patients received a probiotic(Saccharomyces boulardii)for 3 months.RESULTS Presepsin levels were higher in patients with cirrhosis than in healthy individuals[342(91-2875)vs 120(102-141)pg/mL;P=0.048].Patients with elevated presepsin levels accounted for 56.3%of all included patients.They had lower levels of serum albumin and higher levels of serum total bilirubin and overall severity of cirrhosis as assessed using the Child–Pugh scale.Patients with elevated presepsin levels had an increased abundance of the main taxa responsible for bacterial translocation,namely Bacilli and Proteobacteria(including the main class Gammaproteobacteria and the minor taxa Xanthobacteraceae and Stenotrophomonas),and a low abundance of bacteria from the family Lachnospiraceae(including the minor genus Fusicatenibacter),which produce short-chain fatty acids that have a positive effect on intestinal barrier function.The presepsin level directly correlated with the relative abundance of Bacilli,Proteobacteria,and inversely correlated with the abundance of Lachnospiraceae and Propionibacteriaceae.After 3 months of taking the probiotic,the severity of cirrhosis on the Child–Pugh scale decreased significantly only in the group with elevated presepsin levels[from 9(8-11)to 7(6-9);P=0.004],while there were no significant changes in the group with normal presepsin levels[from 8(7-8)to 7(6-8);P=0.123].A high level of presepsin before the prescription of the probiotic was an independent predictor of a greater decrease in Child–Pugh scores(P=0.046),as well as a higher level of the Child–Pugh scale(P=0.042),but not the C-reactive protein level(P=0.679)according to multivariate linear regression analysis.CONCLUSION The level of presepsin directly correlates with the abundance in the gut microbiota of the main taxa that are substrates of bacterial translocation in cirrhosis.This biomarker,in the absence of obvious infection,seems important for assessing the state of the gut–liver axis in cirrhosis and deciding on therapy targeted at the gut microbiota in this disease.
文摘A "leaky gut" may be the cutting edge for the passage of toxins, antigens or bacteria into the body, and may play a pathogenic role in advanced liver cirrhosis and its complications. Plasma endotoxin levels have been admitted as a surrogate marker of bacterial translocation and close relations of endotoxemia to hyperdynamic circulation, portal hypertension, renal, cardiac, pulmonary and coagulation disturbances have been reported. Bacterial overgrowth, increased intestinal permeability, failure to inactivate endotoxin,activated innate immunity are all likely to play a role in the pathological states of bacterial translocation. Therapeutic approach by management of the gut-liver axis by antibiotics, probiotics, synbiotics, prebiotics and their combinations may improve the clinical course of cirrhotic patients. Special concern should be paid on anti-endotoxin treatment. Adequate management of the gut-liver axis may be effective for prevention of liver cirrhosis itself by inhibiting the progression of fibrosis.
基金Supported by Immuron, Nasvax, Plantylight, Desert Labs,Teva Pharmaceutical and ENZO Biochem
文摘Gut flora and bacterial translocation (BT) play important roles in the pathogenesis of chronic liver disease, including cirrhosis and its complications. Intestinal bacterial overgrowth and increased bacterial translocation of gut flora from the intestinal lumen predispose patients to bacterial infections, major complications and also play a role in the pathogenesis of chronic liver disorders. Levels of bacterial lipopolysaccharide, a component of gram-negative bacteria, are increased in the portal and/or systemic circulation in several types of chronic liver disease. Impaired gut epithelial integrity due to alterations in tight junction proteins may be the pathological mechanism underlying bacterial translocation. Preclinical and clinical studies over the last decade have suggested a role for BT in the pathogenesis of nonalcoholic steatohepatitis (NASH). Bacterial overgrowth, immune dysfunction, alteration of the luminal factors, and altered intestinal permeability are all involved in the pathogenesis of NASH and its complications. A better understanding of the cell-specific recognition and intracellular signaling events involved in sensing gut-derived microbes will help in the development of means to achieve an optimal balance in the gut-liver axis and ameliorate liver diseases. These may suggest new targets for potential therapeutic interventions for the treatment of NASH. Here, we review some of the mechanisms connecting BT and NASH and potential therapeutic developments.
