To understand the function of porcine adipocyte-special membrane protein （PAMP） gene and the difference of fat deposition ability among various lean pig breeds, a full-length porcine adipocyte-special membrane prote...To understand the function of porcine adipocyte-special membrane protein （PAMP） gene and the difference of fat deposition ability among various lean pig breeds, a full-length porcine adipocyte-special membrane protein （PAMP） gene was successfully amplified using reverse transcription polymerase chain reaction （RT-PCR） and 5＇-rapid amplification of cDNA end （5＇-RACE）. The open reading frame was 1 587 bp encoding 529 amino acids. The nucleotide sequence of the fulllength PAMP gene was deposited in the GenBank under the accession number EF433431. The PAMP gene mRNA expression was analyzed on three lean pig breeds by quantitative reverse transcription polymerase chain reaction （QRTPCR）. The PAMP gene mRNA levels in YHM （Yorkshire × Hampshire × Meishan） pig and DLY （Duroc × Landrance × Yorkshire） pig were about 0.82 and 0.38 times of that in SW （Shanxi-White） pig, respectively.展开更多
Ten healthy weaning piglets of Landrace x Yorkshire （LY） in good body condition with body weight of （24 ± 1.27） kg were selected, and ten weaning piglets of Berkshire × LY （BLY） and Duroc × LY （...Ten healthy weaning piglets of Landrace x Yorkshire （LY） in good body condition with body weight of （24 ± 1.27） kg were selected, and ten weaning piglets of Berkshire × LY （BLY） and Duroc × LY （DLY） with similar situation were also selected respectively to study the growth performance of different cross combinations. Three groups of experimental pigs were bred with conventional diet. The results showed that the average dry matter intakes of combinations of BLY and DLY were slightly decreased by 4.97% and 5.47% compared with the combination of LY, while average daily gain was significantly increased by 3.13% and 1.14%, and feed-weight ratio was decreased by 7.94% and 6.35%, respectively; economic benefit per pig was increased by 0.61 and 0.41 yuan, respectively. The combinations of BLY and DLY have better growth performance, which can be promoted in the production.展开更多
基金the National Natural Science Foundation of China (20011089)the Fi-nance Department Achievement Transformation Project of Shanxi Province in China (2005)
文摘To understand the function of porcine adipocyte-special membrane protein （PAMP） gene and the difference of fat deposition ability among various lean pig breeds, a full-length porcine adipocyte-special membrane protein （PAMP） gene was successfully amplified using reverse transcription polymerase chain reaction （RT-PCR） and 5＇-rapid amplification of cDNA end （5＇-RACE）. The open reading frame was 1 587 bp encoding 529 amino acids. The nucleotide sequence of the fulllength PAMP gene was deposited in the GenBank under the accession number EF433431. The PAMP gene mRNA expression was analyzed on three lean pig breeds by quantitative reverse transcription polymerase chain reaction （QRTPCR）. The PAMP gene mRNA levels in YHM （Yorkshire × Hampshire × Meishan） pig and DLY （Duroc × Landrance × Yorkshire） pig were about 0.82 and 0.38 times of that in SW （Shanxi-White） pig, respectively.
基金Supported by Research Program of Science and Technology Department of Guizhou Province(QKH[2011]5020)
文摘Ten healthy weaning piglets of Landrace x Yorkshire （LY） in good body condition with body weight of （24 ± 1.27） kg were selected, and ten weaning piglets of Berkshire × LY （BLY） and Duroc × LY （DLY） with similar situation were also selected respectively to study the growth performance of different cross combinations. Three groups of experimental pigs were bred with conventional diet. The results showed that the average dry matter intakes of combinations of BLY and DLY were slightly decreased by 4.97% and 5.47% compared with the combination of LY, while average daily gain was significantly increased by 3.13% and 1.14%, and feed-weight ratio was decreased by 7.94% and 6.35%, respectively; economic benefit per pig was increased by 0.61 and 0.41 yuan, respectively. The combinations of BLY and DLY have better growth performance, which can be promoted in the production.
