目的分析宁夏回族自治区儿童青少年近视流行现状、影响因素及不同学段间的差异。方法采用分层整群随机抽样的方法,于2019年9月至12月,在宁夏回族自治区银川市、吴忠市、石嘴山市、固原市和中卫市,随机抽取8所小学、6所初中、6所高中、4...目的分析宁夏回族自治区儿童青少年近视流行现状、影响因素及不同学段间的差异。方法采用分层整群随机抽样的方法,于2019年9月至12月,在宁夏回族自治区银川市、吴忠市、石嘴山市、固原市和中卫市,随机抽取8所小学、6所初中、6所高中、4所大学的学生为研究对象,小学每个年级抽取5个班级,初中至大学每个年级抽取4个班级,以抽取班级的全体学生作为研究对象,共抽取学生14211人,对其进行问卷调查、体格检查和视力测量。不同学段儿童近视的影响因素采用最小绝对收缩和选择算子(LASSO)联合Logistic回归进行分析,选择贝叶斯信息准则(Bayesian information criterion,BIC)最小的模型为最优模型。结果宁夏回族自治区儿童青少年近视检出率为70.3%,女生高于男生,城市高于乡镇,差异均有统计学意义(均为P<0.001);按学段分层后,随着年级的增加,近视检出率随之升高,小学最低,大学最高,不同学段近视检出率差异有统计学意义(P<0.001)。近视影响因素的LASSO-Logistic回归分析表明,城乡、性别、年龄、目前是否配戴眼镜、每日课间操节数、是否积极参加体力活动和过去6个月是否保持规律活动是小学生近视的影响因素(均为P<0.05);性别、目前是否配戴眼镜是初中生和高中生近视的影响因素(均为P<0.05);目前是否配戴眼镜是大学生近视的影响因素(P<0.05)。结论宁夏回族自治区儿童青少年近视检出率高,不同学段儿童青少年近视影响因素差异明显。配戴眼镜是控制近视的保护因素。应根据儿童青少年所处学段开展有针对性的视力相关知识的健康教育,增强其健康保健意识,提高儿童青少年视力。展开更多
目的构建跟距联合畸形(talocalcaneal coalition)的X线影像组学模型,并检验其对跟距联合畸形的筛查诊断能力。方法回顾性分析2019年1月至2023年3月吉林大学中日联谊医院放射线科200例行踝关节或足部X线检查的患者临床放射资料(跟距联合...目的构建跟距联合畸形(talocalcaneal coalition)的X线影像组学模型,并检验其对跟距联合畸形的筛查诊断能力。方法回顾性分析2019年1月至2023年3月吉林大学中日联谊医院放射线科200例行踝关节或足部X线检查的患者临床放射资料(跟距联合阳性及阴性各100例),手动勾画跟距联合畸形所在影像学区域,基于Python-pyradiomics库初步提取影像组学特征,通过曼-惠特尼U检验及最小绝对收缩和选择算子(least absolute shrinkage and selection operator,LASSO)算法实现数据降维和特征筛选,用支持向量机(support vector machine,SVM)对筛选得到的影像组学特征分类建模,最终以受试者工作特征(receiver operating characteristic,ROC)曲线的曲线下面积(area under the curve,AUC)、精确度、召回率、敏感度、特异度及F1分数评价模型的诊断效能。结果从X线图像中初步提取到105个组学特征,经曼-惠特尼U检验及LASSO算法筛选出7个强相关性特征,最终以SVM分类器所构建模型的测试集AUC值为0.93,精确度、召回率、敏感度、特异度和F1分数分别为88%、85%、93%、92%、88%,对跟距联合畸形有良好的筛查诊断能力。结论基于X线的影像组学模型可作为筛查诊断跟距联合畸形的一种准确高效的无创性工具,帮助临床医师诊断跟距联合畸形。展开更多
Background:Hepatocellular carcinoma(HCC)has a poor long-term prognosis.The competition of circular RNAs(circRNAs)with endogenous RNA is a novel tool for predicting HCC prognosis.Based on the alterations of circRNA reg...Background:Hepatocellular carcinoma(HCC)has a poor long-term prognosis.The competition of circular RNAs(circRNAs)with endogenous RNA is a novel tool for predicting HCC prognosis.Based on the alterations of circRNA regulatory networks,the analysis of gene modules related to HCC is feasible.Methods:Multiple expression datasets and RNA element targeting prediction tools were used to construct a circRNA-microRNA-mRNA network in HCC.Gene function,pathway,and protein interaction analyses were performed for the differentially expressed genes(DEGs)in this regulatory network.In the proteinprotein interaction network,hub genes were identified and subjected to regression analysis,producing an optimized four-gene signature for prognostic risk stratification in HCC patients.Anti-HCC drugs were excavated by assessing the DEGs between the low-and high-risk groups.A circRNA-microRNA-hub gene subnetwork was constructed,in which three hallmark genes,KIF4A,CCNA2,and PBK,were subjected to functional enrichment analysis.Results:A four-gene signature(KIF4A,CCNA2,PBK,and ZWINT)that effectively estimated the overall survival and aided in prognostic risk assessment in the The Cancer Genome Atlas(TCGA)cohort and International Cancer Genome Consortium(ICGC)cohort was developed.CDK inhibitors,PI3K inhibitors,HDAC inhibitors,and EGFR inhibitors were predicted as four potential mechanisms of drug action(MOA)in high-risk HCC patients.Subsequent analysis has revealed that PBK,CCNA2,and KIF4A play a crucial role in regulating the tumor microenvironment by promoting immune cell invasion,regulating microsatellite instability(MSI),and exerting an impact on HCC progression.Conclusions:The present study highlights the role of the circRNA-related regulatory network,identifies a four-gene prognostic signature and biomarkers,and further identifies novel therapy for HCC.展开更多
Background:Colon adenocarcinoma(COAD)is a gastrointestinal malignancy with a high mortality rate.Studies have confirmed the role of immunogenic cell death(ICD)in different cancer types.However,there is a lack of resea...Background:Colon adenocarcinoma(COAD)is a gastrointestinal malignancy with a high mortality rate.Studies have confirmed the role of immunogenic cell death(ICD)in different cancer types.However,there is a lack of research on ICD-related genes(ICD-RGs)in COAD.This study aimed to examine the impact of ICD-RGs on COAD and their interaction with the immune microenvironment.Methods:Using data from The Cancer Genome Atlas and Gene Expression Omnibus databases,we identified 107 ICD-RGs in COAD.Using a one-way Cox regression analysis,we examined the relationship between these ICD-RGs and overall survival in COAD.Results:Following the regression analyses,we identified 14 overall survival-related genes.Furthermore,we examined the predictive impact of the ICD-RGs using the least absolute shrinkage and selection operator regression analysis and developed a nine-genes prognostic model.The Cancer Genome Atlas and Gene Expression Omnibus datasets were used for training and validation.Kaplan-Meier analysis was used to confirm that the high-risk group had a lower survival rate than the low-risk group.Finally,following a multifactorial analysis,we created a prognostic nomogram that integrated clinical data and risk scores.Conclusions:The nine-genes model exhibits robust stability and can provide valuable insights for guiding the development of tumor immunotherapy strategies and personalized drug selection for patients with COAD.展开更多
基于粒计算视角,提出粒化-融合框架下的海量高维数据特征选择算法.运用BLB(Bag of Little Bootstrap)的思想,首先将原始海量数据集粒化为小规模数据子集(粒),然后在每个粒上构建多个自助子集的套索模型,实现粒特征选择,最后,各粒特征选...基于粒计算视角,提出粒化-融合框架下的海量高维数据特征选择算法.运用BLB(Bag of Little Bootstrap)的思想,首先将原始海量数据集粒化为小规模数据子集(粒),然后在每个粒上构建多个自助子集的套索模型,实现粒特征选择,最后,各粒特征选择结果按权重融合、排序,得到原始数据集的有序特征选择结果.人工数据集和真实数据集上的实验表明文中算法对海量高维数据集进行特征选择的可行性和有效性.展开更多
文摘目的分析宁夏回族自治区儿童青少年近视流行现状、影响因素及不同学段间的差异。方法采用分层整群随机抽样的方法,于2019年9月至12月,在宁夏回族自治区银川市、吴忠市、石嘴山市、固原市和中卫市,随机抽取8所小学、6所初中、6所高中、4所大学的学生为研究对象,小学每个年级抽取5个班级,初中至大学每个年级抽取4个班级,以抽取班级的全体学生作为研究对象,共抽取学生14211人,对其进行问卷调查、体格检查和视力测量。不同学段儿童近视的影响因素采用最小绝对收缩和选择算子(LASSO)联合Logistic回归进行分析,选择贝叶斯信息准则(Bayesian information criterion,BIC)最小的模型为最优模型。结果宁夏回族自治区儿童青少年近视检出率为70.3%,女生高于男生,城市高于乡镇,差异均有统计学意义(均为P<0.001);按学段分层后,随着年级的增加,近视检出率随之升高,小学最低,大学最高,不同学段近视检出率差异有统计学意义(P<0.001)。近视影响因素的LASSO-Logistic回归分析表明,城乡、性别、年龄、目前是否配戴眼镜、每日课间操节数、是否积极参加体力活动和过去6个月是否保持规律活动是小学生近视的影响因素(均为P<0.05);性别、目前是否配戴眼镜是初中生和高中生近视的影响因素(均为P<0.05);目前是否配戴眼镜是大学生近视的影响因素(P<0.05)。结论宁夏回族自治区儿童青少年近视检出率高,不同学段儿童青少年近视影响因素差异明显。配戴眼镜是控制近视的保护因素。应根据儿童青少年所处学段开展有针对性的视力相关知识的健康教育,增强其健康保健意识,提高儿童青少年视力。
文摘目的构建跟距联合畸形(talocalcaneal coalition)的X线影像组学模型,并检验其对跟距联合畸形的筛查诊断能力。方法回顾性分析2019年1月至2023年3月吉林大学中日联谊医院放射线科200例行踝关节或足部X线检查的患者临床放射资料(跟距联合阳性及阴性各100例),手动勾画跟距联合畸形所在影像学区域,基于Python-pyradiomics库初步提取影像组学特征,通过曼-惠特尼U检验及最小绝对收缩和选择算子(least absolute shrinkage and selection operator,LASSO)算法实现数据降维和特征筛选,用支持向量机(support vector machine,SVM)对筛选得到的影像组学特征分类建模,最终以受试者工作特征(receiver operating characteristic,ROC)曲线的曲线下面积(area under the curve,AUC)、精确度、召回率、敏感度、特异度及F1分数评价模型的诊断效能。结果从X线图像中初步提取到105个组学特征,经曼-惠特尼U检验及LASSO算法筛选出7个强相关性特征,最终以SVM分类器所构建模型的测试集AUC值为0.93,精确度、召回率、敏感度、特异度和F1分数分别为88%、85%、93%、92%、88%,对跟距联合畸形有良好的筛查诊断能力。结论基于X线的影像组学模型可作为筛查诊断跟距联合畸形的一种准确高效的无创性工具,帮助临床医师诊断跟距联合畸形。
基金This study was supported by grants from the High-level Preresearch Program of Zhejiang Shuren University 2019(KXJ121860)Zhejiang Provincial Natural Science Foundation of China(LQ20H190004)。
文摘Background:Hepatocellular carcinoma(HCC)has a poor long-term prognosis.The competition of circular RNAs(circRNAs)with endogenous RNA is a novel tool for predicting HCC prognosis.Based on the alterations of circRNA regulatory networks,the analysis of gene modules related to HCC is feasible.Methods:Multiple expression datasets and RNA element targeting prediction tools were used to construct a circRNA-microRNA-mRNA network in HCC.Gene function,pathway,and protein interaction analyses were performed for the differentially expressed genes(DEGs)in this regulatory network.In the proteinprotein interaction network,hub genes were identified and subjected to regression analysis,producing an optimized four-gene signature for prognostic risk stratification in HCC patients.Anti-HCC drugs were excavated by assessing the DEGs between the low-and high-risk groups.A circRNA-microRNA-hub gene subnetwork was constructed,in which three hallmark genes,KIF4A,CCNA2,and PBK,were subjected to functional enrichment analysis.Results:A four-gene signature(KIF4A,CCNA2,PBK,and ZWINT)that effectively estimated the overall survival and aided in prognostic risk assessment in the The Cancer Genome Atlas(TCGA)cohort and International Cancer Genome Consortium(ICGC)cohort was developed.CDK inhibitors,PI3K inhibitors,HDAC inhibitors,and EGFR inhibitors were predicted as four potential mechanisms of drug action(MOA)in high-risk HCC patients.Subsequent analysis has revealed that PBK,CCNA2,and KIF4A play a crucial role in regulating the tumor microenvironment by promoting immune cell invasion,regulating microsatellite instability(MSI),and exerting an impact on HCC progression.Conclusions:The present study highlights the role of the circRNA-related regulatory network,identifies a four-gene prognostic signature and biomarkers,and further identifies novel therapy for HCC.
文摘Background:Colon adenocarcinoma(COAD)is a gastrointestinal malignancy with a high mortality rate.Studies have confirmed the role of immunogenic cell death(ICD)in different cancer types.However,there is a lack of research on ICD-related genes(ICD-RGs)in COAD.This study aimed to examine the impact of ICD-RGs on COAD and their interaction with the immune microenvironment.Methods:Using data from The Cancer Genome Atlas and Gene Expression Omnibus databases,we identified 107 ICD-RGs in COAD.Using a one-way Cox regression analysis,we examined the relationship between these ICD-RGs and overall survival in COAD.Results:Following the regression analyses,we identified 14 overall survival-related genes.Furthermore,we examined the predictive impact of the ICD-RGs using the least absolute shrinkage and selection operator regression analysis and developed a nine-genes prognostic model.The Cancer Genome Atlas and Gene Expression Omnibus datasets were used for training and validation.Kaplan-Meier analysis was used to confirm that the high-risk group had a lower survival rate than the low-risk group.Finally,following a multifactorial analysis,we created a prognostic nomogram that integrated clinical data and risk scores.Conclusions:The nine-genes model exhibits robust stability and can provide valuable insights for guiding the development of tumor immunotherapy strategies and personalized drug selection for patients with COAD.
文摘基于粒计算视角,提出粒化-融合框架下的海量高维数据特征选择算法.运用BLB(Bag of Little Bootstrap)的思想,首先将原始海量数据集粒化为小规模数据子集(粒),然后在每个粒上构建多个自助子集的套索模型,实现粒特征选择,最后,各粒特征选择结果按权重融合、排序,得到原始数据集的有序特征选择结果.人工数据集和真实数据集上的实验表明文中算法对海量高维数据集进行特征选择的可行性和有效性.
