This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma （ATR） samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry （GC-MS） w...This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma （ATR） samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry （GC-MS） was applied to establishing the quantitative chemical fingerprints of ATRs. A total of 104 volatile compounds were identified and quantified with the information of mass spectra and retention index （RI）. Furthermore, least absolute shrinkage and selection operator （LASSO）, a sparse regularization method, combined with subsampling was employed to improve the classification ability of partial least squares-discriminant analysis （PLS-DA）. After variable selection by LASSO, three chemical markers,β-elemene, α-selinene and α-asarone, were identified for the discrimination of ATRs from two habitats, and the total classification correct rate was increased from 82.76% to 96.55%. The proposed LASSO-PLS-DA method can serve as an efficient strategy for screening marked chemical components and geo-herbalism research of traditional Chinese medicines.展开更多
目的分析宁夏回族自治区儿童青少年近视流行现状、影响因素及不同学段间的差异。方法采用分层整群随机抽样的方法,于2019年9月至12月,在宁夏回族自治区银川市、吴忠市、石嘴山市、固原市和中卫市,随机抽取8所小学、6所初中、6所高中、4...目的分析宁夏回族自治区儿童青少年近视流行现状、影响因素及不同学段间的差异。方法采用分层整群随机抽样的方法,于2019年9月至12月,在宁夏回族自治区银川市、吴忠市、石嘴山市、固原市和中卫市,随机抽取8所小学、6所初中、6所高中、4所大学的学生为研究对象,小学每个年级抽取5个班级,初中至大学每个年级抽取4个班级,以抽取班级的全体学生作为研究对象,共抽取学生14211人,对其进行问卷调查、体格检查和视力测量。不同学段儿童近视的影响因素采用最小绝对收缩和选择算子(LASSO)联合Logistic回归进行分析,选择贝叶斯信息准则(Bayesian information criterion,BIC)最小的模型为最优模型。结果宁夏回族自治区儿童青少年近视检出率为70.3%,女生高于男生,城市高于乡镇,差异均有统计学意义(均为P<0.001);按学段分层后,随着年级的增加,近视检出率随之升高,小学最低,大学最高,不同学段近视检出率差异有统计学意义(P<0.001)。近视影响因素的LASSO-Logistic回归分析表明,城乡、性别、年龄、目前是否配戴眼镜、每日课间操节数、是否积极参加体力活动和过去6个月是否保持规律活动是小学生近视的影响因素(均为P<0.05);性别、目前是否配戴眼镜是初中生和高中生近视的影响因素(均为P<0.05);目前是否配戴眼镜是大学生近视的影响因素(P<0.05)。结论宁夏回族自治区儿童青少年近视检出率高,不同学段儿童青少年近视影响因素差异明显。配戴眼镜是控制近视的保护因素。应根据儿童青少年所处学段开展有针对性的视力相关知识的健康教育,增强其健康保健意识,提高儿童青少年视力。展开更多
Background:Hepatocellular carcinoma(HCC)has a poor long-term prognosis.The competition of circular RNAs(circRNAs)with endogenous RNA is a novel tool for predicting HCC prognosis.Based on the alterations of circRNA reg...Background:Hepatocellular carcinoma(HCC)has a poor long-term prognosis.The competition of circular RNAs(circRNAs)with endogenous RNA is a novel tool for predicting HCC prognosis.Based on the alterations of circRNA regulatory networks,the analysis of gene modules related to HCC is feasible.Methods:Multiple expression datasets and RNA element targeting prediction tools were used to construct a circRNA-microRNA-mRNA network in HCC.Gene function,pathway,and protein interaction analyses were performed for the differentially expressed genes(DEGs)in this regulatory network.In the proteinprotein interaction network,hub genes were identified and subjected to regression analysis,producing an optimized four-gene signature for prognostic risk stratification in HCC patients.Anti-HCC drugs were excavated by assessing the DEGs between the low-and high-risk groups.A circRNA-microRNA-hub gene subnetwork was constructed,in which three hallmark genes,KIF4A,CCNA2,and PBK,were subjected to functional enrichment analysis.Results:A four-gene signature(KIF4A,CCNA2,PBK,and ZWINT)that effectively estimated the overall survival and aided in prognostic risk assessment in the The Cancer Genome Atlas(TCGA)cohort and International Cancer Genome Consortium(ICGC)cohort was developed.CDK inhibitors,PI3K inhibitors,HDAC inhibitors,and EGFR inhibitors were predicted as four potential mechanisms of drug action(MOA)in high-risk HCC patients.Subsequent analysis has revealed that PBK,CCNA2,and KIF4A play a crucial role in regulating the tumor microenvironment by promoting immune cell invasion,regulating microsatellite instability(MSI),and exerting an impact on HCC progression.Conclusions:The present study highlights the role of the circRNA-related regulatory network,identifies a four-gene prognostic signature and biomarkers,and further identifies novel therapy for HCC.展开更多
功能超网络广泛地应用于脑疾病诊断和分类研究中,而现有的关于超网络创建的研究缺乏解释分组效应的能力或者仅考虑到脑区间组级的信息,这样构建的脑功能超网络会丢失一些有用的连接或包含一些虚假的信息,因此,考虑到脑区间的组结构问题...功能超网络广泛地应用于脑疾病诊断和分类研究中,而现有的关于超网络创建的研究缺乏解释分组效应的能力或者仅考虑到脑区间组级的信息,这样构建的脑功能超网络会丢失一些有用的连接或包含一些虚假的信息,因此,考虑到脑区间的组结构问题,引入sparse group Lasso(sgLasso)方法进一步改善超网络的创建。首先,利用sgLasso方法进行超网络创建;然后,引入两组超网络特有的属性指标进行特征提取以及特征选择,这些指标分别是基于单一节点的聚类系数和基于一对节点的聚类系数;最后,将特征选择后得到的两组有显著差异的特征通过多核学习进行特征融合和分类。实验结果表明,所提方法经过多特征融合取得了87.88%的分类准确率。该结果表明为了改善脑功能超网络的创建,需要考虑到组信息,但不能逼迫使用整组信息,可以适当地对组结构进行扩展。展开更多
<strong>Objective</strong>: This paper aims to explore clinical status and related influence factors of pressure injury (PI) in the elderly inpatients with kidney disease, so as to provide reference for th...<strong>Objective</strong>: This paper aims to explore clinical status and related influence factors of pressure injury (PI) in the elderly inpatients with kidney disease, so as to provide reference for the prevention and treatment of PI in the elderly inpatients with kidney disease. <strong>Methods</strong>: Retrospective collection method is adopted to collect 158 clinical cases of the elderly inpatients with kidney disease aged ≥ 60 in the Nephrology Department, the First Affiliated Hospital of Jinan University from January 2017 to December 2019, and then least absolute shrinkage and selection Operator (LASSO) regression analysis is used to analyze 17 possible influence factors;finally Logistic regression model is established to analyze and screen influence factors of risk. <strong>Results</strong>: 1) Among 158 elderly inpatients with medium and high risk of PI, the incidence of PI is 20.25%;the most common stage of injury is stage I (42.5%);sacrococcygeal (60%) is the high-risk site of pressure injury. 2) LASSO regression analysis shows that history of present respiratory infection/respiratory failure (<em>β </em>= 1.2714. <em>P</em> < 0.05) and hospitalization time (<em>β</em> = 0.4177. <em>P </em>< 0.05) are independent factors influencing PI risk in the elderly inpatients with kidney disease. <strong>Concl</strong><strong>usio</strong><strong>n</strong>: The elderly patients with kidney disease and PI risk are the high incidence population of hospital acquired PI;for the elderly inpatients with kidney disease and having respiratory infection history or respiratory failure, prolonged hospitalization will significantly increase the risk of PI. Therefore, targeted preventive and control measures should be taken to reduce the incidence of PI.展开更多
基金Project(21465016)supported by the National Natural Foundation of China
文摘This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma （ATR） samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry （GC-MS） was applied to establishing the quantitative chemical fingerprints of ATRs. A total of 104 volatile compounds were identified and quantified with the information of mass spectra and retention index （RI）. Furthermore, least absolute shrinkage and selection operator （LASSO）, a sparse regularization method, combined with subsampling was employed to improve the classification ability of partial least squares-discriminant analysis （PLS-DA）. After variable selection by LASSO, three chemical markers,β-elemene, α-selinene and α-asarone, were identified for the discrimination of ATRs from two habitats, and the total classification correct rate was increased from 82.76% to 96.55%. The proposed LASSO-PLS-DA method can serve as an efficient strategy for screening marked chemical components and geo-herbalism research of traditional Chinese medicines.
文摘目的分析宁夏回族自治区儿童青少年近视流行现状、影响因素及不同学段间的差异。方法采用分层整群随机抽样的方法,于2019年9月至12月,在宁夏回族自治区银川市、吴忠市、石嘴山市、固原市和中卫市,随机抽取8所小学、6所初中、6所高中、4所大学的学生为研究对象,小学每个年级抽取5个班级,初中至大学每个年级抽取4个班级,以抽取班级的全体学生作为研究对象,共抽取学生14211人,对其进行问卷调查、体格检查和视力测量。不同学段儿童近视的影响因素采用最小绝对收缩和选择算子(LASSO)联合Logistic回归进行分析,选择贝叶斯信息准则(Bayesian information criterion,BIC)最小的模型为最优模型。结果宁夏回族自治区儿童青少年近视检出率为70.3%,女生高于男生,城市高于乡镇,差异均有统计学意义(均为P<0.001);按学段分层后,随着年级的增加,近视检出率随之升高,小学最低,大学最高,不同学段近视检出率差异有统计学意义(P<0.001)。近视影响因素的LASSO-Logistic回归分析表明,城乡、性别、年龄、目前是否配戴眼镜、每日课间操节数、是否积极参加体力活动和过去6个月是否保持规律活动是小学生近视的影响因素(均为P<0.05);性别、目前是否配戴眼镜是初中生和高中生近视的影响因素(均为P<0.05);目前是否配戴眼镜是大学生近视的影响因素(P<0.05)。结论宁夏回族自治区儿童青少年近视检出率高,不同学段儿童青少年近视影响因素差异明显。配戴眼镜是控制近视的保护因素。应根据儿童青少年所处学段开展有针对性的视力相关知识的健康教育,增强其健康保健意识,提高儿童青少年视力。
基金This study was supported by grants from the High-level Preresearch Program of Zhejiang Shuren University 2019(KXJ121860)Zhejiang Provincial Natural Science Foundation of China(LQ20H190004)。
文摘Background:Hepatocellular carcinoma(HCC)has a poor long-term prognosis.The competition of circular RNAs(circRNAs)with endogenous RNA is a novel tool for predicting HCC prognosis.Based on the alterations of circRNA regulatory networks,the analysis of gene modules related to HCC is feasible.Methods:Multiple expression datasets and RNA element targeting prediction tools were used to construct a circRNA-microRNA-mRNA network in HCC.Gene function,pathway,and protein interaction analyses were performed for the differentially expressed genes(DEGs)in this regulatory network.In the proteinprotein interaction network,hub genes were identified and subjected to regression analysis,producing an optimized four-gene signature for prognostic risk stratification in HCC patients.Anti-HCC drugs were excavated by assessing the DEGs between the low-and high-risk groups.A circRNA-microRNA-hub gene subnetwork was constructed,in which three hallmark genes,KIF4A,CCNA2,and PBK,were subjected to functional enrichment analysis.Results:A four-gene signature(KIF4A,CCNA2,PBK,and ZWINT)that effectively estimated the overall survival and aided in prognostic risk assessment in the The Cancer Genome Atlas(TCGA)cohort and International Cancer Genome Consortium(ICGC)cohort was developed.CDK inhibitors,PI3K inhibitors,HDAC inhibitors,and EGFR inhibitors were predicted as four potential mechanisms of drug action(MOA)in high-risk HCC patients.Subsequent analysis has revealed that PBK,CCNA2,and KIF4A play a crucial role in regulating the tumor microenvironment by promoting immune cell invasion,regulating microsatellite instability(MSI),and exerting an impact on HCC progression.Conclusions:The present study highlights the role of the circRNA-related regulatory network,identifies a four-gene prognostic signature and biomarkers,and further identifies novel therapy for HCC.
文摘功能超网络广泛地应用于脑疾病诊断和分类研究中,而现有的关于超网络创建的研究缺乏解释分组效应的能力或者仅考虑到脑区间组级的信息,这样构建的脑功能超网络会丢失一些有用的连接或包含一些虚假的信息,因此,考虑到脑区间的组结构问题,引入sparse group Lasso(sgLasso)方法进一步改善超网络的创建。首先,利用sgLasso方法进行超网络创建;然后,引入两组超网络特有的属性指标进行特征提取以及特征选择,这些指标分别是基于单一节点的聚类系数和基于一对节点的聚类系数;最后,将特征选择后得到的两组有显著差异的特征通过多核学习进行特征融合和分类。实验结果表明,所提方法经过多特征融合取得了87.88%的分类准确率。该结果表明为了改善脑功能超网络的创建,需要考虑到组信息,但不能逼迫使用整组信息,可以适当地对组结构进行扩展。
文摘<strong>Objective</strong>: This paper aims to explore clinical status and related influence factors of pressure injury (PI) in the elderly inpatients with kidney disease, so as to provide reference for the prevention and treatment of PI in the elderly inpatients with kidney disease. <strong>Methods</strong>: Retrospective collection method is adopted to collect 158 clinical cases of the elderly inpatients with kidney disease aged ≥ 60 in the Nephrology Department, the First Affiliated Hospital of Jinan University from January 2017 to December 2019, and then least absolute shrinkage and selection Operator (LASSO) regression analysis is used to analyze 17 possible influence factors;finally Logistic regression model is established to analyze and screen influence factors of risk. <strong>Results</strong>: 1) Among 158 elderly inpatients with medium and high risk of PI, the incidence of PI is 20.25%;the most common stage of injury is stage I (42.5%);sacrococcygeal (60%) is the high-risk site of pressure injury. 2) LASSO regression analysis shows that history of present respiratory infection/respiratory failure (<em>β </em>= 1.2714. <em>P</em> < 0.05) and hospitalization time (<em>β</em> = 0.4177. <em>P </em>< 0.05) are independent factors influencing PI risk in the elderly inpatients with kidney disease. <strong>Concl</strong><strong>usio</strong><strong>n</strong>: The elderly patients with kidney disease and PI risk are the high incidence population of hospital acquired PI;for the elderly inpatients with kidney disease and having respiratory infection history or respiratory failure, prolonged hospitalization will significantly increase the risk of PI. Therefore, targeted preventive and control measures should be taken to reduce the incidence of PI.
