AIM: To investigate whether non-canonical autophagy transport receptor cell cycle progression 1(CCPG1) is involved in the corneal antifungal immune response.METHODS: Human corneal epithelial cells(HCECs) and human mye...AIM: To investigate whether non-canonical autophagy transport receptor cell cycle progression 1(CCPG1) is involved in the corneal antifungal immune response.METHODS: Human corneal epithelial cells(HCECs) and human myeloid leukemia mononuclear cells(THP-1) macrophages stimulated by Aspergil us fumigatus(A. fumigatus) were used as cell models. The expression of CCPG1 m RNA was detected by q RT-PCR. Western blot was used to determine the protein expression of CCPG1 and interleukin-1β(IL-1β). The dectin-1 neutralizing antibody was used to detect the association between dectin-1 and CCPG1. Immunofluorescence was used to observe the colocalization of CCPG1 and C-type lectin-like receptor-1(CLEC-1) in THP-1 macrophages.RESULTS: The expression of CCPG1 started to increase at 4 h after infection and increased in a time-dependent manner in HCECs and THP-1 macrophages. With dectin-1 neutralizing antibody pretreatment, the expression of IL-1β was down-regulated. CCPG1 up-regulation in response to A. fumigatus infection was independent of dectin-1. Immunofluorescence showed the colocalization of CCPG1 and CLEC-1 in THP-1 macrophages.CONCLUSION: As a specific autophagy protein of noncanonical autophagy pathway, CCPG1 is involved in corneal infection with A. fumigatus.展开更多
Lectin-like oxidized low-density lipoprotein receptor-1(LOX-1)have recently been identified to be closely related to the occurrence and development of atherosclerosis(AS).A growing body of evidence has suggested Chine...Lectin-like oxidized low-density lipoprotein receptor-1(LOX-1)have recently been identified to be closely related to the occurrence and development of atherosclerosis(AS).A growing body of evidence has suggested Chinese medicine takes unique advantages in preventing and treating As.In this review,the related research progress of AS and LOX-1 has been summarized.And the anti-As effects of 10 active components of herbal medicine through LOX-1 regulation have been further reviewed.As a potential biomarker and target for intervention in AS,LOX-1 targeted therapy might provide a promising and novel approach to atherosclerotic prevention andtreatment.展开更多
基金Supported by the National Natural Science Foundation of China(No.82171019)the Natural Science Foundation of Shandong Province(No.ZR2021MH368)+1 种基金Traditional Chinese Medicine Research Project of Qingdao(No.2020-zyy055)Shandong Qingdao Outstanding Health Professional Development Fund。
文摘AIM: To investigate whether non-canonical autophagy transport receptor cell cycle progression 1(CCPG1) is involved in the corneal antifungal immune response.METHODS: Human corneal epithelial cells(HCECs) and human myeloid leukemia mononuclear cells(THP-1) macrophages stimulated by Aspergil us fumigatus(A. fumigatus) were used as cell models. The expression of CCPG1 m RNA was detected by q RT-PCR. Western blot was used to determine the protein expression of CCPG1 and interleukin-1β(IL-1β). The dectin-1 neutralizing antibody was used to detect the association between dectin-1 and CCPG1. Immunofluorescence was used to observe the colocalization of CCPG1 and C-type lectin-like receptor-1(CLEC-1) in THP-1 macrophages.RESULTS: The expression of CCPG1 started to increase at 4 h after infection and increased in a time-dependent manner in HCECs and THP-1 macrophages. With dectin-1 neutralizing antibody pretreatment, the expression of IL-1β was down-regulated. CCPG1 up-regulation in response to A. fumigatus infection was independent of dectin-1. Immunofluorescence showed the colocalization of CCPG1 and CLEC-1 in THP-1 macrophages.CONCLUSION: As a specific autophagy protein of noncanonical autophagy pathway, CCPG1 is involved in corneal infection with A. fumigatus.
基金Supported by the National Natural Science Foundation of China(No.81874432 and No.82074369)Science and Technology Planning Project of Guangdong Province(No.2018B030322012)。
文摘Lectin-like oxidized low-density lipoprotein receptor-1(LOX-1)have recently been identified to be closely related to the occurrence and development of atherosclerosis(AS).A growing body of evidence has suggested Chinese medicine takes unique advantages in preventing and treating As.In this review,the related research progress of AS and LOX-1 has been summarized.And the anti-As effects of 10 active components of herbal medicine through LOX-1 regulation have been further reviewed.As a potential biomarker and target for intervention in AS,LOX-1 targeted therapy might provide a promising and novel approach to atherosclerotic prevention andtreatment.
