Atypical Cutaneous Leishmaniasis by Leishmania Mexicana: A Case Report with Dermoscopic, Histopathological and Molecular Study

Cutaneous leishmaniasis is a neglected tropical skin endemic disease, with worldwide distribution. By 2020 in the Americas, Mexico was in 12th place with 11 states reporting new cases. Molecular biology with different targets for diagnosis and species identification has been used for decades, also dermoscopy, a non-invasive diagnostic tool has shown its usefulness. We present the first case of cutaneous leishmaniasis in non-endemic place (Guerrero, Mexico) identifying Leishmania mexicana with molecular biology, and treated with itraconazole.

墨西哥利什曼原虫环形DNA1含有编码核苷酸结合蛋白基因(英文)

目的 对扩增的墨西哥利什曼原虫环形 DNA1(CD1)部分片段进行测序 ,确定具有蛋白编码功能的开放阅读框。 方法 通过脉冲电泳方法分离并使用琼脂糖酶方法回收 CD1,酶切的 CD1片段克隆于 p Zero载体 ,用 M13通用引物自动测序决定核苷酸序列 ,用 GCG- PCGENE程序分析序列。 结果 测出 4385个核苷酸序列 ,通过分析发现两个开放阅读框具有蛋白质编码功能 (编码核苷酸结合蛋白 )。 结论 墨西哥利什曼原虫