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LeJA2基因沉默表达载体导入番茄转化系统的建立
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作者 刘媛媛 张春庆 吴承来 《山东农业科学》 2010年第2期16-19,共4页
试验设侵染时间、菌液浓度和侵染温度3个因素,利用根癌农杆菌将LeJA2基因按L9(34)正交表转化番茄。结果表明,当菌液浓度为OD260=0.6、侵染温度40℃、侵染10 min时的转化效果较好,从而建立了较优的LeJA2基因沉默表达载体导入番茄的转化体... 试验设侵染时间、菌液浓度和侵染温度3个因素,利用根癌农杆菌将LeJA2基因按L9(34)正交表转化番茄。结果表明,当菌液浓度为OD260=0.6、侵染温度40℃、侵染10 min时的转化效果较好,从而建立了较优的LeJA2基因沉默表达载体导入番茄的转化体系,并已得到14株转化再生的番茄植株,经卡那抗性筛选和PCR检测,证明LeJA2-RNA i基因已导入番茄并表达。 展开更多
关键词 leja2基因 根癌农杆菌 番茄 转化系统
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Bayesian Model Calibration with Interpolating Polynomials based on Adaptively Weighted Leja Node
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作者 Laurent van den Bos Benjamin Sanderse +1 位作者 Wim Bierbooms Gerard van Bussel 《Communications in Computational Physics》 SCIE 2020年第1期33-69,共37页
An efficient algorithm is proposed for Bayesian model calibration,which is commonly used to estimate the model parameters of non-linear,computationally expensive models using measurement data.The approach is based on ... An efficient algorithm is proposed for Bayesian model calibration,which is commonly used to estimate the model parameters of non-linear,computationally expensive models using measurement data.The approach is based on Bayesian statistics:using a prior distribution and a likelihood,the posterior distribution is obtained through application of Bayes’law.Our novel algorithm to accurately determine this posterior requires significantly fewer discrete model evaluations than traditional Monte Carlo methods.The key idea is to replace the expensive model by an interpolating surrogate model and to construct the interpolating nodal set maximizing the accuracy of the posterior.To determine such a nodal set an extension to weighted Leja nodes is introduced,based on a new weighting function.We prove that the convergence of the posterior has the same rate as the convergence of the model.If the convergence of the posterior is measured in the Kullback–Leibler divergence,the rate doubles.The algorithm and its theoretical properties are verified in three different test cases:analytical cases that confirm the correctness of the theoretical findings,Burgers’equation to show its applicability in implicit problems,and finally the calibration of the closure parameters of a turbulence model to show the effectiveness for computa-tionally expensive problems. 展开更多
关键词 Bayesian model calibration INTERPOLATION leja nodes surrogate modeling
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基于分数阶梯度算子的图像匹配算法 被引量:3
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作者 付万程 达飞鹏 黄源 《图学学报》 CSCD 北大核心 2017年第3期352-360,共9页
为保护更多的图像细节特征,提出一种基于分数阶梯度算子构建非线性尺度空间的图像匹配算法。在非线性扩散滤波方程的传导函数中,引入分数阶梯度算子,利用快速显式扩散(FED)算法构建非线性尺度空间,采用Leja排序法对一个FED周期内的时间... 为保护更多的图像细节特征,提出一种基于分数阶梯度算子构建非线性尺度空间的图像匹配算法。在非线性扩散滤波方程的传导函数中,引入分数阶梯度算子,利用快速显式扩散(FED)算法构建非线性尺度空间,采用Leja排序法对一个FED周期内的时间步长重新排序,采用一种新的基于Hessian矩阵的关键点检测算子检测关键点,利用局部灰度序模式(LIOP)描述子对关键点进行描述。实验结果表明,与其他经典算法相比,该算法在各种图像变换下具有更好的性能。 展开更多
关键词 图像匹配 分数阶梯度算子 快速显式扩散 leja排序 局部灰度序模式
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Weakly Admissible Meshes and Discrete Extremal Sets 被引量:1
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作者 Len Bos Stefano De Marchi +1 位作者 Alvise Sommariva Marco Vianello 《Numerical Mathematics(Theory,Methods and Applications)》 SCIE 2011年第1期1-12,共12页
We present a brief survey on (Weakly) Admissible Meshes and corresponding Discrete Extremal Sets, namely Approximate Fekete Points and Discrete Leja Points.These provide new computational tools for polynomial least s... We present a brief survey on (Weakly) Admissible Meshes and corresponding Discrete Extremal Sets, namely Approximate Fekete Points and Discrete Leja Points.These provide new computational tools for polynomial least squares and interpolationon multidimensional compact sets, with different applications such as numerical cubature, digital filtering, spectral and high-order methods for PDEs. 展开更多
关键词 Weakly admissible meshes Approximate Fekete points Discrete leja points
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