Inhibition of H_(2)O_(2)-induced apoptosis of GC2-spg cells by functionalized selenium nanoparticles with lentinan through ROSmediated ERK/p53 signaling pathways

AH_(2)O_(2)-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of seleniumcontaining antioxidant enzymes induced by oxidative stress.A safe and effective nano-drug system of functionalized selenium-containing nanoparticles(SeNPs)was developed with lentinan(LNT)(SeNPs@LNT).Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT(1,2,4,8,16,32μM)for 24-72 h to evaluate the cytotoxicity of selenium.GC2-spg cells were randomly divided into the following groups:control,hydrogen peroxide(H_(2)O_(2)),SeNPs@LNT,and H_(2)O_(2)+SeNPs@LNT groups.H_(2)O_(2)+SeNPs@LNT group was pretreated with SeNPs@LNT 4μM for 12 h,followed by H_(2)O_(2)600μM for 8 h.The cell viability decreased in the H_(2)O_(2) group and increased significantly in the SeNPs@LNT group.Compared with the H_(2)O_(2) group,the SeNPs@LNT+H_(2)O_(2) group exhibited obvious red fluorescence,indicating a higher level of mitochondrial membrane potential.The content of intracellular reactive oxygen species(ROS)in the SeNPs@LNT group reduced significantly,and the intensity of green fluorescence in the SeNPs@LNT+H_(2)O_(2) group decreased significantly compared with the H_(2)O_(2) group,indicating the inhibitory effect of SeNPs@LNT on the generation of ROS-induced oxidative stress.The activity of GPx and SOD increased significantly in the SeNPs@LNT group.The expression of p53 decreased significantly under the intervention of nano-selenium,and GPx1 expression increased.In the oxidative stress group,the expressions of DNA damage-related proteins and apoptosis-related proteins were higher than those in other groups.Thus,SeNPs@LNT can promote GC2-spg cell proliferation,improve GPx and SOD activities,remove intracellular ROS,and reduce mitochondrial damage and functional abnormalities caused by oxidative stress by regulating the ERK and p53 protein levels.SeNPs@LNT has good biological activity and antioxidant effect,which can be used to protect the male reproductive system from oxidative stress.

Inhibition of H_(2)O_(2)-induced TM3 cell apoptosis by oxidative stress by lentinan functionalized selenium nanoparticles through JAK2/STAT-3 and P53 pathways

Background:Nano-selenium has been widely used in antiviral and anticancer therapy,and has the advantages of good targeting and low toxicity.For the first time,we combined male reproduction with nano-selenium to investigate its antioxidant effect.This study investigated the protective effect of lentinan functionalized selenium nanoparticles on oxidative stress injury of the hydrogen peroxide(H_(2)O_(2))-induced Leydig cell line,TM3.Methods:The suitable concentration of nano-selenium treatment to promote cell proliferation was also discussed.The concentration of 4μM could significantly promote the growth of TM3 cells.Oxidative stress damage was caused using an 800μM concentration of hydrogen peroxide.The cells were divided into four groups:normal control group,oxidative stress treatment group,H_(2)O_(2)+SeNPs@LNT group,and SeNPs@LNT group.The H_(2)O_(2)+SeNPs@LNT group was pretreated with 4μM of SeNPs@LNT for 12 h,followed by 800μM of H_(2)O_(2)for 8 h.Results:Nano-selenium could significantly promote the proliferation and viability of TM3 cells.SeNPs@LNT treatment increased the level of mitochondrial membrane potential in normal cells and slowed down the decline in mitochondrial membrane potential level caused by oxidative stress injury.In addition,the increase in reactive oxygen species caused by oxidative stress was inhibited by SeNPs@LNT treatment.The apoptosis of TM3 cells was detected,and SeNPs@LNT alleviated the necrosis and apoptosis of TM3 cells induced by H_(2)O_(2).Nano-selenium plays a protective role against oxidative H_(2)O_(2)-induced stress injury in TM3 cells through the changes in the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway and P53 pathway,and the expression levels of other related proteins,protein kinase B(AKT)and C3.Conclusion:SeNPs@LNT exhibited good biological activity and antioxidant effect and can thus be used to protect the male reproductive system from oxidative stress.

Advances in Research of Biological Activity,Action Mechanism and Structure-Activity Relationship of Lentinan

Lentinula edodes is the second largest edible mushroom in the world and is widely used as food and medicine.Modern research shows that lentinan(LNT)is the main active component of L.edodes.It has anti-cancer,treatment of diabetes,intestinal protection,anti-inflammatory,anti-oxidation,anti-aging,hepatoprotective,immune-regulating effects.In this review,the biological activity,action mechanism and structure-activity relationship of LNT in recent years are reviewed.On this basis,the existing problems were discussed,and the future research and application of LNT were prospected.Finally,it is hoped that this review will promote the in-depth study of LNT and provide a reference for its development as a drug and functional food.

Lentinan administration alleviates diarrhea of rotavirus-infected weaned pigs via regulating intestinal immunity

Background:Lentinan(LNT)may regulate many important physiological functions of human and animals.This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus(RV)-challenged weaned pigs.Methods:Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d(n=14).RV infection was executed on d 15.After extracting polysaccharides from LNT product,its major monosaccharides were analyzed.Then,LNT polysaccharide was used to administrate RV-infected IPEC-J2 cells.Results:Dietary LNT supplementation supported normal function of piglets even when infected with RV,as reflected by reduced growth performance loss and diarrhea prevalence,and maintained gut immunity(P<0.05).The polysaccharide was isolated from LNT product,which molecular weight was 5303 Da,and major monosaccharides included glucose,arabinose and galactose.In RV-infected IPEC-J2 cells,this polysaccharide significantly increased cell viability(P<0.05),and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides(P<0.05).Conclusion:Those results suggest that LNT administration increases the piglets’resistance to RV-induced stress,likely by supporting intestinal immunity.

香菇多糖Lentinan的提取分离、生物活性和构效关系的研究进展

香菇多糖Lentinan是从香菇中分离出来的具有重要生物活性的物质,文章系统地阐述了近年来有关香菇多糖Lentinan的提取分离纯化方法、生物活性、结构与活性关系等方面的研究进展,并展望了今后香菇多糖Lentian的研究方向。

Lentinan prolonged survival in patients with gastric cancer receiving S-1-based chemotherapy

AIM:To examine whether administration of lentinan,purifiedβ-1,3-glucan,can prolong survival in advanced gastric cancer patients receiving S-1-based chemotherapy.METHODS:Since 2004,78 patients with metastatic or recurrent gastric cancer have received S-1-based chemotherapy as first-line treatment.Survival,side effects,and the ratio of granulocytes/lymphocytes(G/L ratio)were compared between 2 groups of patients who received chemo-immunotherapy using lentinan and chemotherapy alone.RESULTS:Median overall survival was significantly longer in the former group than in the latter group[689 d(95%CI:431-2339 d)vs 565 d(95%CI:323-662 d),P=0.0406].In addition,the G/L ratio in patients who received lentinan was maintained around or below 2,which was significantly lower than that in patients who received chemotherapy alone(P<0.001).CONCLUSION:Chemo-immunotherapy with lentinan offers a significant advantage over S-1-based chemotherapy alone in terms of survival in patients with advanced gastric cancer.

Rapid Quantification of Bioactive Lentinan with an Aniline Blue Fluorescent Method

Lentinan is a clinically approved immune modulator and its anticancer and immunomodulatory bioactivity is found to be dependent on its triple helical conformation. Therefore, the development of rapid and convenient method for determination of bioactive lentinan with triple helical conformation holds great promise for the quality control of lentinan healthy products. In this work, an aniline blue fluorescent method was optimized and established to accurately and rapidly detect bioactive lentinan. In the presence of lentinan, the fluorescence intensity of aniline blue with 404 nm excitation and 492 nm emission dramatically enhanced within 15 min in pH 10 glycine-NaOH buffer solution, which allowed the analysis of lentinan in a very simple and fast manner. The method allowed for the sensitive determination of lentinan in the range of 1 to 60 μg/mL with a detection limit of 0.25 μg/mL. Notably, the protocol exhibited excellent selectivity for the determination of triple helical lentinan over other saccharides. The method was successfully applied to the detection of bioactive lentinan in health tonic solution, which demonstrated the method had great potential for quality control of lentinan contained products.

Effects of lentinan and thymopentin combined with adjuvant chemotherapy on immunity, oxidative stress, matrix metalloproteinases and related factors in patients with tongue squamous cell carcinoma

Objective:To investigate the effects of lentinan and thymopentin combined with adjuvant chemotherapy on immunity, oxidative stress, matrix metalloproteinases and related factors in patients with tongue squamous cell carcinoma.Methods: Retrospective analysis of 78 patients with tongue squamous cell carcinoma admitted to our hospital from February 2013 to November 2017, according to the postoperative chemotherapy scheme, the patients were divided into control group (n=42) and observation group (n=36). The patients in control group were given PF chemotherapy scheme (cisplatin + compound fluorouracil injection), on this basis, the patients in observation group were given lentinan and thymopentin injection combined treatment. The immune index, oxidative stress, matrix metalloproteinase, cyclin D1 (CyclinD1) and B lymphocyte tumor-2 (BCL-2) were detected and analyzed before and after treatment.Results:After treatment, the immunoglobulin level in the control group was not statistically significant compared with that before treatment (P>0.05);the levels of IgA, IgG and IgM in the observation group were significantly higher than those before treatment (P<0.05), and the levels of IgA, IgG and IgM in the observation group were significantly higher than those in the control group (P<0.05);the level of SOD in control group was significantly lower than that before treatment (P<0.05), and the level of MDA was significantly higher than that before treatment (P<0.05);the observation group was the opposite, and its SOD level was significantly higher than that of the control group (P<0.05), and the MDA level was significantly lower than the control group (P<0.05);the levels of MMP-2 and MMP-9 in the two groups were significantly lower than those before treatment (P<0.05), and the levels of MMP-2 and MMP-9 in the observation group were significantly lower than those in the control group (P<0.05);the levels of CyclinD1 and BCL-2 in the two groups were significantly lower than those before treatment (P<0.05), and the levels of CyclinD1 and BCL-2 in the observation group were significantly lower than those in the control group (P<0.05).Conclusions:Lentinan and thymopentin assisted PF chemotherapy regimen can enhance the immune function of patients with tongue squamous cell carcinoma after radical surgery, improve oxidative stress response, inhibit tumor cell proliferation and metastasis. It has the significance of clinical popularization.

香菇多糖在水产养殖中的应用研究进展

香菇多糖是从香菇子实体中提取的一种多糖类物质,研究证明其能提高动物机体的免疫水平和抗氧化能力,改善肠道健康等功能。文章从香菇多糖的结构与生物活性、对水产养殖动物抗氧化、免疫、抗病害能力和肠道健康等方面的研究进展进行综述,以期为香菇多糖在水产养殖产业中的应用提供参考。

香菇多糖的生物活性及在鸡、猪养殖中的应用研究进展

香菇多糖(Lentinan,LNT)是香菇的主要活性成分之一,是典型的真菌β-葡聚糖,其单糖组成包括甘露糖、半乳糖和葡萄糖等,立体结构呈三螺旋,该结构使LNT具备免疫调节、抗氧化、促肠道健康、抗炎和抗肿瘤等多种生物活性,广泛应用于医学、食品、养殖等领域。LNT作为饲料添加剂已进入《饲料添加剂品种目录》。本文主要综述了LNT的生物活性及在鸡、猪生产中的应用研究进展,以期为LNT作为新型饲料添加剂应用于鸡、猪养殖提供思路和理论支撑。

香菇多糖对ApoE^(-/-)小鼠动脉粥样硬化斑块形成及AMPK信号通路的影响

目的:探讨香菇多糖(LNT)对ApoE^(-/-)小鼠动脉粥样硬化(AS)斑块形成及单磷酸腺苷活化蛋白激酶(AMPK)信号通路的影响。方法:ApoE^(-/-)小鼠分为模型组、立普妥组(5 mg/kg)、LNT低(5 mg/kg)、中(10 mg/kg)、高(20 mg/kg)剂量组,以相似遗传背景的C57BL/6小鼠为对照组。灌胃给药12周后,全自动生化分析仪检测血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)水平;ELISA检测血清IL-6、IL-1β水平;油红O染色观察整体主动脉斑块形成情况;HE染色观察主动脉根部斑块形成情况;蛋白免疫印迹检测主动脉AMPK通路蛋白表达。结果:与对照组比较,模型组血清TC、TG、LDL-C、IL-6、IL-1β水平、主动脉斑块面积、主动脉根部斑块面积、主动脉组织NLRP3、IL-6、IL-1β表达显著升高,主动脉组织p-AMPK/AMPK表达和血清HDL-C水平降低(P<0.05);与模型组比较,立普妥组、LNT中、高剂量组TC、TG、LDL-C、IL-6、IL-1β、主动脉斑块面积、主动脉根部斑块面积、主动脉组织NLRP3、IL-6、IL-1β表达显著降低,主动脉组织p-AMPK/AMPK表达和血清HDL-C水平显著升高,且LNT各剂量组间差异有统计学意义(P<0.05);立普妥组与LNT高剂量组差异无统计学意义(P>0.05)。结论:LNT可能通过激活AMPK通路抑制AS小鼠炎症反应和AS斑块形成。

Protective Effects of Lentinan against T Lymphocytes Injury in Mice under Chronic Radiation Stress

Objective To study the effects of lentinan (LTN) on mice exposed to chronic radiation. Methods Animals were divided into three groups (n = 10), they were animals exposed to radiation (Rad), normal control animals (Ctr), and irradiated animals treated with LTN (Rad + LTN). Animal model of chronic radiation stress injury was induced by irradiating mice with 60 Co γ-ray for 6 weeks from Monday to Friday consecutively. Before radiation, the mice in Rad + LTN group were ip injected with 0.5 mL LTN (0.01 mg/mL), whereas mice in other groups were injected with 0.9% physiological saline. The effects of LTN treatment on irradiated mice were examined by histological analysis on the spleen. The cell numbers and viability of T lymphocytes, which were isolated from the spleen, were determined by Trypan blue staining. Nitric oxide (NO) production and interleukin-2 (IL-2) secretion in T lymphocytes were also measured. Results Chronic radiation significantly reduced the body weights and the spleen and thymus indexes, associated with reduced T lymphocytes viability and functions, and elevated NO production. Treatment with LTN significantly normalized the elevated NO production, and attenuated the negative outcomes resulting from radiation mentioned above. Conclusion The results suggest that radioprotective effect of LTN may be contributed by improved T lymphocytes viability and functions via regulating the NO and IL-2 production in T lymphocytes.

香菇多糖对蛋鸡生长性能、免疫功能及禽流感疫苗免疫效果的影响

为掌握香菇多糖对蛋鸡生长性能、免疫功能及禽流感疫苗免疫效果的影响,试验将120只1日龄禽流感非免疫罗曼灰蛋鸡随机分为4组,对照组不添加香菇多糖,试验Ⅰ、Ⅱ、Ⅲ组分别添加50、100、200 mg/kg香菇多糖进行试验,试验为期77 d。结果显示,Ⅰ、Ⅱ、Ⅲ平均日增重显著高于对照组(P<0.05),料重比显著低于对照组(P<0.05),Ⅲ组最佳;Ⅲ组蛋鸡血清中IL-2和IFN-γ水平显著高于对照组(P<0.05);禽流感疫苗免疫后,Ⅰ、Ⅱ、Ⅲ组蛋鸡禽流感H5抗体水平均显著高于对照组(P<0.05),Ⅲ组抗体水平最高。结果表明,香菇多糖可以提高蛋鸡生长性能、免疫功能及禽流感疫苗免疫效果,在本试验条件下,香菇多糖的最佳添加量为200 mg/kg。

Im munomodulatory and Therapeutic Effect of Lentinan in Treating Condyloma Acuminatum

Objective: To observe the immunomodulatory and therapeutic effect of lentinan in treating condyloma acuminatum (CA). Methods: Thirty six CA patients were randomly divided into two groups, 19 in the treated group treated with lentinan and CO 2 laser irradiation, and 17 in the control group, treated with laser irradiation alone. Their T lymphocyte subsets of peripheral blood and level of serum interleukin 2 (IL 2) and soluble interleukin 2 receptor (SIL 2R) were determined before and after treatment, and the recurrence rates of the two groups were compared. Results: After treatment, in the treated group, the CD 4/CD 8 ratio, serum IL 2 raised and serum SIL 2R lowered significantly (P <0.05, 0.05, 0.05 respectively) as compared with before treatment, while those parameters were not changed significantly in the control group. The recurrence rate of the treated group was lower than that of the control group. P <0.05. Conclusion: Lentinan could modulate the cellular immunofunction of CA patients and reduce the recurrence rate of CA cases.

香菇多糖基凝胶面膜的制备及其性能研究

以香菇多糖、凝胶基质为原料,制备了食用菌补水面膜,通过正交试验得出面膜较优配方,并研究其感官、质构、LF-NMR、流变、抗氧化和皮肤水分测试.结果表明:筛选的羧甲基纤维素钠(CMC-Na)为最佳凝胶基质;单因素和正交试验得出香菇复合多糖凝胶面膜的最优配方包括:CMC-Na 3.3 g,香菇多糖1 g,甘油4 mL,山梨醇2 g,山梨酸钾1 g, VC 1 g,去离子水100 mL.香菇多糖凝胶面膜的弹性和对照组无显著性差异,粘聚性以及凝胶强度高于对照组,其凝胶强度为18.142 g;香菇多糖凝胶面膜与对照面膜中均含有自由水;流变分析表明香菇多糖面膜凝胶的粘性模量高于对照组;香菇多糖面膜凝胶的抗氧化能力高于对照组,并且DPPH自由基清除率随着面膜质量浓度增大而增大,最佳浓度为5 mg/mL;保湿率高于芦荟胶面膜,但略低于睡眠面膜,在2 h时保湿率最佳值是99.46%;面部皮肤水分含量结果表明,香菇多糖面膜凝胶对不同的人都有显著的保湿效果,试用者的面部皮肤水分含量从28.314%提高至33.472%.香菇多糖凝胶面膜的开发为最大限度地发挥香菇多糖的价值提供了一个全新的思路.

香菇多糖对黄羽肉鸡生长性能及免疫功能的影响

试验旨在研究饲料中添加香菇多糖对黄羽肉鸡免疫功能及生长性能的影响。选取1日龄体重接近的黄羽肉鸡180只,随机分为6个组,每个组3个重复,每个重复10只鸡。空白对照组:饲喂基础饲粮;25 mg/kg香菇多糖组:在基础饲粮中添加25 mg/kg的香菇多糖;50 mg/kg香菇多糖组:在基础饲粮中添加50 mg/kg香菇多糖;100 mg/kg香菇多糖组:在基础饲粮中添加100 mg/kg香菇多糖;150 mg/kg香菇多糖组:在基础饲粮中添加150 mg/kg香菇多糖;200 mg/kg香菇多糖组:在基础饲粮中添加200 mg/kg香菇多糖,试验期为42 d。结果表明,在饲料中连续14~42 d添加150~200 mg/kg的香菇多糖可以显著提高黄羽肉鸡血清一氧化氮(NO)、白介素-2(IL-2)、干扰素-γ(IFN-γ)、溶菌酶(LZM)含量和免疫器官指数(P<0.05),在饲料中连续42 d添加50~150 mg/kg可显著提高黄羽肉鸡的平均日增重、特定生长率和饲料转化率(P<0.05)。综上,在饲料中添加适宜剂量的香菇多糖可以显著提高黄羽肉鸡血清NO、IL-2、IFN-γ、LZM含量和免疫器官指数,从而提高机体免疫功能;可显著提高黄羽肉鸡平均日增重、特定生长率和饲料转化率,从而改善生长性能,推荐添加剂量为150 mg/kg,推荐添加时间为42 d。

Meta Analysis of Lentinan Injection plus Cisplatin in Treatment of Malignant Pleural Effusion

Objective To evaluate the efficacy and safety of lentinan injection plus cisplatin(LIC)in the treatment of malignant pleural effusion(MPE).Methods We searched the database of Cochrane Library,PubMed,EMBASE, ISI Web of Knowledge,Chinese Biomedical Literature Database,Chinese Scientific Journals Full-text Database, Chinese Journal Full-text,and Google Scholar,etc.,up to February 28th,2011 to identify randomized controlled trials(RCTs)about lentinan injection(LI)for MPE,evaluate the quality of the included studies,and analyze the data by Cochrane Collaboration’s RevMan5.0 software.Results Twenty-nine RCTs involving 1831 patients were included.Meta analysis results suggested that there were some differences when comparing LIC with control groups suffering from MPE,for LIC could improve the near-term curative effect and the quality of life to some extent. Besides,compared with chemotherapy alone,LI plus chemotherapy had an advantage in relieving adverse reactions, such as gastrointestinal reactions,myelosuppression,chest pain,and general malaise.Conclusion The current evidence indicates that LI may have adjuvant therapeutic effects for MPE.

香菇多糖通过IL-6/STAT3/Notch信号通路调控胰腺癌细胞增殖、迁移及化疗敏感性

目的:香菇多糖(Lentinan,LNT)已被证明对癌细胞具有直接的细胞毒性。然而,这种抗肿瘤作用机制尚不清楚。本研究旨在探讨香菇多糖对胰腺癌细胞增殖、迁移及化疗敏感性的影响及其机制。方法:将胰腺癌Capan-1细胞随机分为对照组、IL-6组、IL-6+LNT组,MTT检测细胞存活率,Western blot检测p-STAT3、STAT3、Notch1和Hes1表达水平,Transwell实验检测细胞迁移。建立顺铂耐药细胞模型(Capan-1/DDP),检测各组顺铂半抑制浓度(IC50),分析Ki-67、MMP-9、MRP1和P-gp蛋白相对表达水平。选择Capan-1/DDP细胞株进行裸鼠成瘤实验,验证LNT对裸鼠体内瘤体的瘤重、IL-6/STAT3/Notch信号通路的影响。结果:7.5~240μg/m L香菇多糖对胰腺癌细胞增殖有抑制作用;与IL-6组比较,IL-6+LNT组增殖率显著降低,p-STAT3/STAT3、Notch1和Hes1表达和迁移水平(极)显著下调(P<0.05,P<0.01);与IL-6组比较,IL-6+LNT组Capan-1/DDP细胞株增殖率极显著下调,IC50极显著降低,Ki-67、MMP-9、MRP1和P-gp表达水平显著下调(P<0.05,P<0.01)。体内实验显示,与IL-6组比较,顺铂+IL-6组、LNT+IL-6组瘤体体积、瘤重极显著下调,p-STAT3/STAT3、Notch1、Hes1表达水平(极)显著降低(P<0.05,P<0.01)。结论:LNT抑制胰腺癌Capan-1细胞增殖、迁移和耐药,与调控IL-6/STAT3/Notch信号通路相关。

香菇多糖锌螯合物的制备及结构表征和体外抗氧化活性研究

目的制备香菇多糖锌螯合物(lentinan-znic chelate,LNT-Zn),对其结构进行表征,并考查其体外抗氧化活性。方法以香菇为原料,将提取纯化的香菇多糖与乙酸锌反应制备LNT-Zn,通过紫外光谱、傅里叶变换红外光谱(Fourier transform infrared spectroscopy,FTIR)和场发射扫描电子显微镜(scanning electron microscopy,SEM)对其结构进行表征,以原子分光光度法测定的LNT-Zn中锌离子质量分数为指标,采用单因素试验和响应面试验优化LNT-Zn制备条件,最后测定其对羟基自由基(·OH、1,1-二苯基-2-三硝基苯肼自由基)(1,1-diphenyl-2-picrylhydrazyl radical,DPPH·)的清除率和总抗氧化活性等指标,研究LNT-Zn的抗氧化活性。结果LNT-Zn制备最佳条件为:香菇多糖与乙酸锌的比例为1 g:8.68 mmoL、反应温度为49℃、反应时间为236min、反应体系的pH为5.02;在此条件下,可制得锌离子含量为56.08mg/g(相对标准偏差为0.69%)的LNT-Zn。紫外光谱和红外光谱结果表明,香菇多糖中O-H、C-H、C-O-C和C=O等官能团与锌离子发生螯合。·OH和DPPH·清除试验和总抗氧化活性测定结果表明,LNT-Zn的抗氧化能力优于香菇多糖。结论本研究建立的LNT-Zn的合成工艺切实可行,可用于LNT-Zn的规模化合成,可为香菇深加工及产品研发提供思路与理论参考。