The complete sequence of the 18S ribosomal RNA gene （18S rRNA） from Lymantria dispar was cloned and analysed here. 18S rRNA and mitochondrial cytochrome c oxidasel （coxl） gene sequences of Lymantria dispar were co...The complete sequence of the 18S ribosomal RNA gene （18S rRNA） from Lymantria dispar was cloned and analysed here. 18S rRNA and mitochondrial cytochrome c oxidasel （coxl） gene sequences of Lymantria dispar were compared with homologous sequences of other nine insects from different orders. Analytic results showed that 18S rRNA of these insects had two conserved domains and the second domain was an even more conserved region. The phylogenetic trees based on the full-length sequence and the second domain fragment of 18S rRNA as well as sequence of coxl from different orders indicated that Lepidoptera and Trichoptera, which belongs to Amphiesmenoptera, had a closer phylogenetic relationship and fewer differences were observed comparing with traditional taxonomic results.展开更多
Objective:To identify the biological forms,sporozoite rate and molecular characterization of the Anopheles stephensi(An.stephensi)in Hormozgan and Sistan-Baluchistan provinces,the most important malarious areas in Ira...Objective:To identify the biological forms,sporozoite rate and molecular characterization of the Anopheles stephensi(An.stephensi)in Hormozgan and Sistan-Baluchistan provinces,the most important malarious areas in Iran.Methods:Wild live An.stephensi samples were collected from different malarious areas in southern Iran.The biological forms were identified based on number of egg-ridges.Molecular characterization of biological forms was verified by analysis of the mitochondrial cytochrome oxidase subunit I and II(mtDNA-COI/COII).The Plasmodium infection was examined in the wild female specimens by species-specific nested-PCR method.Results:Results showed that all three biological forms including mysorensis,intermediate and type are present in the study areas.Molecular investigations revealed no genetic variation between mtDNA COI/COII sequences of the biological forms and no Plasmodium parasites was detected in the collected mosquito samples.Conclusions:Presence of three biological forms with identical sequences showed that the known biological forms belong to a single taxon and the various vectorial capacities reported for these forms are more likely corresponded to other epidemiological factors than to the morphotype of the populations.Lack of malaria parasite infection in An,stephensi,the most important vector of malaria,may be partly due to the success and achievement of ongoing active malaria control program in the region.展开更多
基金supported by the National Natural Science Foundation of China(31101490)
文摘The complete sequence of the 18S ribosomal RNA gene （18S rRNA） from Lymantria dispar was cloned and analysed here. 18S rRNA and mitochondrial cytochrome c oxidasel （coxl） gene sequences of Lymantria dispar were compared with homologous sequences of other nine insects from different orders. Analytic results showed that 18S rRNA of these insects had two conserved domains and the second domain was an even more conserved region. The phylogenetic trees based on the full-length sequence and the second domain fragment of 18S rRNA as well as sequence of coxl from different orders indicated that Lepidoptera and Trichoptera, which belongs to Amphiesmenoptera, had a closer phylogenetic relationship and fewer differences were observed comparing with traditional taxonomic results.
基金financially supported by Tehran University of Medical Sciences(TUMS)(Grant No.89-01-27-10448)
文摘Objective:To identify the biological forms,sporozoite rate and molecular characterization of the Anopheles stephensi(An.stephensi)in Hormozgan and Sistan-Baluchistan provinces,the most important malarious areas in Iran.Methods:Wild live An.stephensi samples were collected from different malarious areas in southern Iran.The biological forms were identified based on number of egg-ridges.Molecular characterization of biological forms was verified by analysis of the mitochondrial cytochrome oxidase subunit I and II(mtDNA-COI/COII).The Plasmodium infection was examined in the wild female specimens by species-specific nested-PCR method.Results:Results showed that all three biological forms including mysorensis,intermediate and type are present in the study areas.Molecular investigations revealed no genetic variation between mtDNA COI/COII sequences of the biological forms and no Plasmodium parasites was detected in the collected mosquito samples.Conclusions:Presence of three biological forms with identical sequences showed that the known biological forms belong to a single taxon and the various vectorial capacities reported for these forms are more likely corresponded to other epidemiological factors than to the morphotype of the populations.Lack of malaria parasite infection in An,stephensi,the most important vector of malaria,may be partly due to the success and achievement of ongoing active malaria control program in the region.
