DNA Barcoding:An Effective Tool for Species Identification of Lepidopteran Oak Pests

[Objective] The paper was to improve the efficiency and accuracy of early forecast of Lepidopteran oak-infesting pests.[Method] DNA barcoding technique was established for quick species identification using mitochondrial cytochrome C oxidase subunit Ⅰ（COⅠ） as the standard gene.This barcoding technique was used to amplify and sequence genomic DNA samples from eggs and pupae of 11 species of Lepidopteran pests collected from oak.[Result] The DNA barcoding standard genes of 594-708 bp were determined from eggs and pupae of Lepidopteran insects.There were differences of 0-2 bases in DNA barcode sequences between conspecific eggs and pupae,with the sequence identity of 99.7%-100%.The average content of A,T,G and C of DNA barcode sequences from Lepidopteran insects were 30.7%,38.5%,14.9% and 15.9%,respectively.The obtained DNA barcode sequences had 91.4%-100% identity and 0-8.6% difference degree with GenBank-deposited DNA barcode sequences from organisms of the genetically-closest relationship.Among them,DNA barcode sequences from egg and pupa samples of 10 Lepidopteran insects（No.1-20） had 99%-100% identity and 0-1.0% difference degree with homologous sequences in GenBank database,while the remaining samples（No.21-22） had high difference degree（8.6%） with homologous sequences.[Conclusion] The established DNA barcoding technique is an effeetive tool for species identification of Lepidopteran pests using genomic DNA from eggs and pupae of Lepidopteran insects.

Control Effects of Ten Kinds of Insecticides on Main Lepidopteran Pests of Cauliflower

[Objective]The paper was to screen out effective insecticides against main lepidopteran pests of cauliflower.[Method]Field trials were carried out in 2018,and 11 treatments were designed in the test.The population decline rate and corrected control effect were calculated.[Result]16%Indoxacarb+tebufenozide EC and 35%methoxyfenozide+indoxacarb SC had quick bioactivity and persistence,and the control effects at 1 and 10 d post spraying were greater than 85%.11%Emamectin benzoate+lufenuron ME,1 billion PIB/mL of Autographa californica NPV SC and 10%alpha-cypermethrin+lufenuron EC had good persistence,and the control effect at 10 d post spraying was greater than 90%.[Conclusion]Compound preparations of indoxacarb and tebufenozide or methoxyfenozide are recommended for controlling lepidopteran pests of cauliflower.

Verification of Glyphosate Resistance, Lepidopteran Resistance and Wide Compatibility of Male Sterile Line E1C4008S in Rice

Weeds and insect pests are two important biotic stresses resulting in yield loss in rice, and wide compatibility is the essential characteristic of breeding inter-subspecific hybrid rice. This study focused on glyphosate resistance, lepidopteran resistance and wide compatibility as well as identification of molecular and some agronomic characteristics of transgenic male sterile line E1 C4008 S. The results indicated that glyphosate resistance gene Epsps^# and lepidopteran resistance gene Cry1 ca^# were transferred into japonica wide compatibility male sterile line 4008 S by Agrobacterium-mediated method, and four independent transformation events named E1 C4008 S-1, E1 C4008 S-2, E1 C4008 S-3 and E1 C4008 S-4 were obtained, in which E1 C4008 S-3 and E1 C4008 S-4 were of single copy insertion. The EPSPS protein contents of E1 C4008 S-3 and E1 C4008 S-4 in different organs were significantly different both in descending order of leaf > stem > root, and ranged from 300.58 to 1410.69 μg/g at the tillering stage. The glyphosate tolerable concentration(dosage) of E1 C4008 S-3 and E1 C4008 S-4 reached at least 16 g/L(54.42 kg/hm^2), and the seeds of E1 C4008 S-4 can germinate normally on the medium containing 1 g/L glyphosate. The CRY1 C protein contents of E1 C4008 S-3 and E1 C4008 S-4 in different organs were significantly different both in descending order of leaf > stem > root, and ranged from 0.62 to 2.43 μg/g at the tillering stage. The larvae mortalities of rice leaf rollers fed on leaves of E1 C4008 S-3 and E1 C4008 S-4 for 5 d were 95.35% and 97.77%, respectively, while the average mortalities of silkworms fed with protein extracts from leaves of E1 C4008 S-3 and E1 C4008 S-4 reached 94.55% and 83.64%, respectively. The results suggested that wide compatibility and evaluated agronomic traits of E1 C4008 S-4 were not significantly changed by insertion of the exogenous genes. Overall, a novel male sterile germplasm with glyphosate resistance, lepidopteran resistance and wide compatibility was verified to be developed in rice.

桃园主要鳞翅目害虫性诱芯的混用诱捕效果

【目的】明确北方桃园内梨小食心虫、苹小卷叶蛾、桃蛀螟和桃潜叶蛾等4种害虫性诱芯之间的互作效应。【方法】通过田间诱芯混合诱捕试验,比较4种害虫性诱芯之间的相互作用。【结果】2022年和2023年调查发现,单个梨小食心虫性诱芯与各梨小食心虫性诱芯组合在梨小食心虫诱捕数量上均无显著差异。单个桃潜叶蛾性诱芯与各桃潜叶蛾性诱芯组合在桃潜叶蛾诱捕数量上也均无显著差异。苹小卷叶蛾性诱芯+梨小食心虫性诱芯组合对苹小卷叶蛾的诱捕数量均显著低于各年单独使用苹小卷叶蛾性诱芯时的诱捕数量(p<0.05)。【结论】在诱捕梨小食心虫时,梨小食心虫性诱芯可以与桃潜叶蛾、苹小卷叶蛾、桃蛀螟等3种害虫性诱芯混用;在诱捕桃潜叶蛾时,桃潜叶蛾性诱芯可以与梨小食心虫、苹小卷叶蛾、桃蛀螟等3种害虫性诱芯混用;在诱捕苹小卷叶蛾时,苹小卷叶蛾性诱芯可以与桃潜叶蛾和桃蛀螟这两种害虫的性诱芯混用,不能与梨小食心虫性诱芯混用。

Long-term seasonal fluctuations of lepidopteran cereal stemborers and their natural enemies on maize and wild host plants in southern Benin

The seasonal fluctuation oflepidoptcran cereal stemborers on maize and wild host plants （i.e., grasses and a few sedges） was investigated in southern Benin from 1988 to 1998 by time series analysis and repeated-measures analysis of variance （ANOVA）. In addition, a walk-in light trap was used to study the flight behavior of adult moths. On both cereals and grasses, the noctuid Sesamia calaraistis was the predominant species, followed by the pyralid Eldana saccharina. The noctuid Busseolafusca was rare on both maize and wild host plants. In general, pest populations increased during the course of the year to reach peak densities during the second short rainy season, and then crashed to close to zero during the dry season. On wild host plants, egg masses and other immature stages were collected throughout the year but they were higher on wild grasses than maize during the off-season. Thus wild host plants can be considered as refuge for both borers and natural enemies during the off-season, when maize is not available. However, only four out of the eleven wild host species played a discernable role. S. calamistis egg densities appeared to be influenced by density-dependent factors, suggesting an effect of natural enemies. Temperature and rainfall had a negative effect on egg abundance. Larval parasitism by a Kenyan strain of the braconid Cotesia sesamiae, which was released in southern Benin in the early 1990s, and by the tachinid Sturmiopsisparasitica varied between seasons and years but there were no discernable patterns. For both parasitoids and borer host species, parasitism was positively correlated with trap catches of adult moths. The recovery of C. sesamiae during a 2-year period suggests that the parasitoid has established its population in southern Benin.

Phytopesticidal effects of Spilanthes acmella(L.)Murr.leaves on three economically important lepidopteran insect pests

Objective:To evaluate the antifeedant and larvicidal activities of dichloromethane(DCM),acetone,dimethylsulfoxide(DMSO)and aqueous extracts of Spilanthes acmella(S.acmella)leaves against Earias vitella,Helicoverpa armigera and Spodoptera litura.Methods:DCM,acetone,DMSO and aqueous extracts were studied using fruit and leaf discs no-choice method at 0.625%,1.25%,2.5%and 5%concentration.Results:All the extracts showed antifeedant and larvicidal activities against all the tested insects.However,maximum antifeedant activity was detected in DCM extract of S.acmella against all tested pest,followed by acetone,DMSO and aqueous extracts at 5%concentration.The leaves extract of S.acmella also showed larvicidal activity;maximum larval mortality was observed in DCM extract against Helicoverpa armigera(48.88%),Spodoptera litura(44.88%)and Earias vitella(75.11%)followed by acetone,DMSO and aqueous extracts at 5%concentration.Conclusions:DCM extract of S.acmella could be further purified to develop a formulation for eco-friendly pest control agent.

苏云金杆菌微型颗粒剂对玉米鳞翅目害虫的防治效果

包括草地贪夜蛾、亚洲玉米螟、棉铃虫在内的鳞翅目害虫是安徽省夏玉米生产上发生最重、为害最大的一类虫害,为探索其高效绿色的防控技术,分别于心叶初期和心叶末期,施用两种苏云金杆菌G033A和PS3微型颗粒剂,研究其对玉米鳞翅目害虫防治效果、心叶保护效果及产量影响。结果表明:心叶初期以人工撒施方式,按300、450、600 g/667 m~2剂量施药后7 d,G033A微型颗粒剂对害虫的防效分别为71.62%、77.18%和81.54%,PS3微型颗粒剂的防效分别为75.00%、83.73%和86.91%;G033A微型颗粒剂对玉米心叶保护效果分别为59.42%、68.86%和71.72%,PS3微型颗粒剂分别为65.08%、72.64%和75.50%,持效期均可达14 d。心叶末期以无人机撒施方式,按照1、1.5、2 kg/667 m~2剂量施药后7 d,G033A微型颗粒剂对害虫的防效分别为76.90%、81.22%和86.02%,PS3微型颗粒剂分别为81.71%、84.89%和89.20%,药后14d防效均开始下降;挽回产量损失率G033A微型颗粒剂分别为5.17%、7.07%和9.09%,PS3微型颗粒剂分别为5.56%、7.47%和9.57%。相对于可湿性粉剂,微型颗粒剂产品持效期更长、防治效果更好;相同剂量处理下,药后7、14 d,PS3微型颗粒剂防效均高于G033A微型颗粒剂。G033A和PS3微型颗粒剂防治玉米鳞翅目害虫较好,可有效挽回产量损失,人工撒施推荐剂量均为300~600 g/667 m~2、无人机撒施推荐剂量均为1~2 kg/667 m~2。

鳞翅目昆虫性别决定机制研究进展

昆虫性别决定机制复杂多样,研究昆虫性别决定机制既能加强对物种进化关系的理解,也能为通过遗传调控控制害虫种群提供新方向。鳞翅目昆虫多为雌雄二型,繁殖方式以两性生殖为主,性染色体系统大多为ZW/ZZ系统,雌性为ZW型,雄性为ZZ型。昆虫性别决定是通过级联反应调控的,在鳞翅目昆虫ZW/ZZ系统中,位于W染色体上的性别决定初始信号沉默雄性化关键基因Masculinizer(Masc),然后Doublesex(dsx)基因被雌性特异性剪接,从而调控雌性偏向基因的表达;而雄虫中Masc正常表达,调控dsx进行雄性特异剪接,实现雄性分化。本文对鳞翅目昆虫性别决定初始信号、Masc和dsx的研究现状,以及基于基因编辑技术和性别决定机制在鳞翅目昆虫遗传调控中的应用进行了综述。相关研究为鳞翅目昆虫的性别筛选、不育昆虫释放技术(sterile insect technique,SIT)和释放携带雌性特异显性致死基因昆虫技术(female specific release of insects carrying dominant lethal,fsRIDL)奠定理论基础。

直链鳞翅目信息素的特点及欧盟登记管理政策概况

信息素农药中,鳞翅目昆虫性信息素种类最多,研究最为详细。其中,直链鳞翅目信息素类别包含了大部分常见的、已知的信息素,并且国内外登记的信息素农药产品大多数活性成分均属于直链鳞翅目信息素。直链鳞翅目信息素具有低毒性、低暴露的特性,很多国家和国际组织大大减少对于直链鳞翅目信息素的登记数据需求。欧盟作为有着较丰富信息素农药管理经验的国际组织,对直链鳞翅目信息素的登记和监管制定了完善的法规和细则。本文整理并概述了直链鳞翅目信息素特点、欧盟对该类信息素的批准登记活性成分以及相关管理政策,同时整理了我国信息素农药的登记情况及要求,旨在为直链鳞翅目信息素的进一步研究应用和登记管理提供参考。

两种虫酰肼类新化合物对五种鳞翅目害虫的生物活性

为了比较虫酰肼及其衍生物对鳞翅目5种害虫的毒力,以虫酰肼为对照药剂,分别采用浸叶法、浸虫法和点滴法测定了虫酰肼衍生物0593(Ⅲf)和0673对甜菜夜蛾Spodoptera exigua(Hbner)、斜纹夜蛾Prodenia litura(Fabricius)、小菜蛾Plutella xylostella(Linnaeus)、棉铃虫Helicoverpa armigera(Hbner)和玉米螟Ostrinia furnacalis(Guen e)的毒力;通过浸叶法系统研究了虫酰肼和虫酰肼衍生物0593对甜菜夜蛾生长发育的影响。结果表明:0593和0673对甜菜夜蛾4龄幼虫的触杀毒力分别是虫酰肼的11.3倍和7.4倍,对斜纹夜蛾4龄幼虫触杀毒力分别是虫酰肼的30.4倍和24.7倍,对小菜蛾3龄幼虫浸虫处理的毒力分别是虫酰肼的4.7倍和4.5倍,对棉铃虫3龄幼虫的触杀毒力分别是虫酰肼的15.5倍和15.2倍,对玉米螟4龄幼虫的触杀毒力分别是虫酰肼的1.3倍和2.0倍,较虫酰肼的毒力都有很大提高,而0593和0673对5种害虫的毒力差异不显著。用虫酰肼和0593处理甜菜夜蛾2龄幼虫,都能导致存活幼虫陆续死亡;化蛹率和正常蛹数降低;成虫羽化率、单雌产卵量和卵的孵化率显著减少,对后代的繁殖力影响显著;在相同处理浓度下,0593较虫酰肼对甜菜夜蛾后代繁殖力的不利影响更大。因此,0593是较虫酰肼杀虫毒力更高、对生长发育和繁殖力不利影响更大的药剂,开发应用价值很大。

米满对几种重要鳞翅目害虫室内生物活性的研究

米满是非固醇结构的蜕皮激素活性的新一代杀虫剂，室内研究表明米满对粘虫、 玉米螟、小菜蛾、斜纹夜蛾、棉铃虫均有较好的杀虫活性，其中对粘虫的杀虫效果最好。 米满的杀虫作用较慢，５ｄ后杀虫效果好于对照药剂氰戊菊酯和辛硫磷，对其药效的评价 应在药后５ｄ和７ｄ。米满对玉米螟、粘虫、棉铃虫试虫表现出明显的抑制生长和拒食作用。

四种Bt蛋白对六种重要鳞翅目害虫的杀虫活性评价

筛选对靶标害虫高效、低毒的Bt蛋白对未来开发新型Bt生物杀虫剂、转基因抗虫植物新品种等具有重要意义。我们在室内测定了Cry2Ab4、Cry2Ah1、Cry1Ca7及Vip3Aa11四种Bt蛋白对棉铃虫、甜菜夜蛾、斜纹夜蛾、黏虫、玉米螟、二化螟的杀虫效果。结果表明,Cry2Ab4杀虫谱广、对六种鳞翅目的重要害虫都具有很高的杀虫效果,尤其是对棉铃虫、甜菜夜蛾、斜纹夜蛾和玉米螟的毒力效果显著;Cry2Ah1对斜纹夜蛾、甜菜夜蛾具有很好的致死作用,对棉铃虫、玉米螟和二化螟也具有较好的杀虫效果,但对黏虫只有明显的体重抑制作用、致死效果不明显;Cry1Ca7对甜菜夜蛾和斜纹夜蛾有明显的防治效果,其次对棉铃虫、二化螟也有较好的杀虫作用,但对玉米螟毒力效果较差,对黏虫没有毒力作用;Vip3Aa11对甜菜夜蛾和斜纹夜蛾的防治效果好,对玉米螟、棉铃虫和二化螟有较好的防治效果,但对黏虫没有毒力作用。

鳞翅目昆虫肠道微生物的多样性及其与宿主的相互作用

作为地球上数量最多的动物群体,昆虫种类繁多、形态各异,其肠道内也栖息着大量的微生物,这些微生物直接或间接对宿主发挥着重要的作用,如参与消化、吸收利用营养物质、合成信息素,同时在抵御外来病原的侵入与定殖、提高宿主免疫活性的过程中也起着关键作用。鳞翅目是昆虫纲中仅次于鞘翅目的第二大目,其中既有严重危害农作物的害虫,又有能够授粉和产生经济效益的益虫,对生态系统和人类影响深远。近年来,随着新方法、新技术在肠道微生态学上的应用,国内外鳞翅目昆虫肠道微生物的研究也日趋热烈。本文将围绕家蚕Bombyx mori、海灰翅夜蛾Spodoptera littoralis、小菜蛾Plutella xylostella等几种代表性鳞翅目昆虫,介绍目前为止针对鳞翅目昆虫肠道微生物组的研究,包括肠道的内环境、微生物的多样性及其研究方法,随后对已明确的鳞翅目昆虫肠道微生物的组成,以及共生菌具体对宿主代谢解毒、免疫健康等方面做出的贡献进行分析,以期为进一步深入探究鳞翅目昆虫肠道微生物打下基础,以及转化这些知识来控制害虫(海灰翅夜蛾、小菜蛾等),促进益虫(家蚕等)生长,确保农业和经济更好地发展。

8个烟碱类杀虫剂生物活性比较研究

比较吡虫啉、啶虫脒、烯啶虫胺、噻虫啉、噻虫嗪、噻虫胺、氯噻啉、呋虫胺等8个烟碱类杀虫剂的生物活性，为科学应用提供参考。采用浸渍法、浸稻苗法、浸叶碟法等对豆蚜、褐飞虱、二化螟、小菜蛾进行室内毒力测定，对活性进行分组，并进行了结构与活性关系初步分析。结果表明：烟碱类药剂对豆蚜LC50值为0．0314～0．3505mg／L，对抗吡虫啉的褐飞虱LC50值为0．5657～49．4046mg／L，对二化螟LC50值为7．5176～139．0369mg／L，对小菜蛾LC50值为10．5334～100mg／L。烟碱基闭环与开环、取代杂环等结构特点对药剂活性有影响。烟碱类药剂对同翅目害虫的活性显著优于对鳞翅目害虫的活性。在实际应用中可以采取轮用策略和兼治策略推进药剂的科学合理使用，还可加强研发种子处理剂等。

非甾醇蜕皮激素类杀虫剂的研究进展

非甾醇蜕皮激素类杀虫剂是近年来开发的新型、高效、具有蜕皮激素活性的昆虫生长调节剂。它引起昆虫特别是鳞翅目幼虫早熟 ,使幼虫蜕皮致死。此外 ,对一些昆虫还具有化学绝育性和神经中毒作用。非甾醇蜕皮激素类杀虫剂和蜕皮激素一样 ,作用于蜕皮激素受体 ,但由于在细胞中的稳定存在 ,致使一些基因不能正常表达 ,羽化激素的释放受到抑制 。

苜蓿银纹夜蛾核型多角体病毒对草地贪夜蛾幼虫的杀虫活性及生防效果

为明确苜蓿银纹夜蛾核型多角体病毒(Autographa californica multiple nucleopolyhedrovirus,AcMNPV)对草地贪夜蛾幼虫的防治效果,采用室内生物活性测定、田间药效试验等方法,研究和分析AcMNPV对草地贪夜蛾幼虫的杀虫活性和生防效果,结果显示,AcMNPV对草地贪夜蛾2龄幼虫的LC_(50)=2.9×10^(7)PIB/mL;在田间,AcMNPV+Bt复配制剂1千万苜核·苏云菌悬浮剂(1500 mL/hm^(2))药后第10天的平均虫口防效为68.99%,而药后第15天的平均防效亦有66.87%,均对草地贪夜蛾幼虫表现出较好的防治效果。应用DNAMAN 6.0软件对试验后死虫样进行DNA同源比对鉴定,结果显示polh、lef-8和lef-9基因序列同源性均达到100%。以上结果表明AcMNPV对草地贪夜蛾幼虫有较好的控制作用。建议在草地贪夜蛾低龄幼虫发生高峰期施用1千万苜核·苏云菌悬浮剂,最好选择在晴天16:00-17:00使用,避开高温和光照等不利环境条件的影响,以使病毒制剂能更好地发挥作用,提高其防治效果。

在雌不发生交换的鳞翅目类昆虫中使用F2群体作图显隐性分子标记连锁图谱的极大似然(ML)法

在鳞翅目昆虫中，雌性个体的减数分裂细胞不发生遗传重组．这类物种的杂交F2群体中杂合子基因型的与一般物种中雌雄个体的减数分裂细胞都发生遗传重组的F2群体杂合子表型不同．由于这个原因，作图这类物种的遗传连锁图谱通常是使用回交群体．但是，用回交群体作图所得的图谱是不完整的，因为图谱上所有的标记都是非轮回亲本提供的，因此标记数不会超过杂交F2群体的一半．另外，目前还没有任何方法和软件可以用杂交F2群体来作图鳞翅目类昆虫，如家蚕等连锁图谱．为了解决这一问题，本文发展了用F2群体作图这类昆虫连锁图谱的两点和三点极大似然法，同时还给出了大群体作图形态基因连锁图谱的一般方法．

转Cry1Ia基因抗虫大豆对鳞翅目靶标害虫的抗性分析

以转Cry1Ia基因抗虫大豆株系KE1为试验材料,分析其遗传稳定性及鳞翅目靶标害虫抗性,计算实验室内和田间环境下,甜菜夜蛾、斜纹夜蛾、黏虫和大豆食心虫叶面积损失率、幼虫平均校正死亡率、平均体重抑制百分率和虫食率。结果表明,KE1株系中目的基因Cry1Ia和标记基因Bar在T3~T5代中连续三代稳定遗传。KE1对靶标害虫斜纹夜蛾(S.litura)、甜菜夜蛾(S.exigua)、粘虫(M.separata)和大豆食心虫(Leguminivora glycinivorella Matsumura)等鳞翅目表现抗虫,对照植株垦农18表现感虫,说明在垦农18中Cry1Ia基因表达可提高受体垦农18对鳞翅目害虫抗虫水平。

鳞翅目昆虫基因组中微卫星DNA的特征以及对其分离的影响

本文根据我们对鳞翅目昆虫棉铃虫和松毛虫以及其它动物 (筏蜘蛛、朱、鳕鱼和飞蝗 )的微卫星富集性基因组DNA文库的筛选和分析结果 ,结合其它实验室已发表的资料 ,对鳞翅目昆虫基因组中微卫星DNA的丰度和结构特点进行了较为系统的分析。结果表明 :与其它类群相比 ,尽管鳞翅目昆虫物种间存在差异 ,但其基因组中存在明显偏多的侧翼序列重复的、以多拷贝形式存在的微卫星位点 ,且其中相当一部分以基因家族的形式存在。微卫星DNA家族通常可以在序列分析阶段被识别出来 ,但很多多拷贝位点只有通过一系列后续分析才能被检查出来。这应是鳞翅目昆虫中微卫星位点的优化率相对偏低的主要原因。棉铃虫和松毛虫基因组中三相重复微卫星丰度相对较高 ,从而从某种程度上补偿了这些物种微卫星分离过程中因丰度低、多拷贝位点比例高所带来的困难。棉铃虫微卫星DNA家族侧翼序列中多聚T/A序列的存在表明 。

表达Cry1Ab和Cry2Ab蛋白的转基因玉米GAB-3对四种主要鳞翅目害虫的抗性评价

为评价表达Cry1Ab和Cry2Ab蛋白的转基因玉米GAB-3对黏虫Mythimna separata(Walker)、亚洲玉米螟Ostrinia furnacalis(Guenée)、棉铃虫Helicoverpa armigera(Hübner)和草地贪夜蛾Spodoptera frugiperda(J. E. Smith)的杀虫效果,采用室内生测的方法研究了GAB-3不同组织器官对以上四种害虫存活率的影响,并通过田间接虫试验进一步测试了GAB-3对黏虫和亚洲玉米螟的抗性。室内生测结果显示:转基因玉米GAB-3心叶期叶片对黏虫、亚洲玉米螟、棉铃虫和草地贪夜蛾具有很强的毒杀作用,苞叶、穗尖、穗轴、花丝、雄穗和籽粒对亚洲玉米螟和棉铃虫具有显著的抗性。田间接虫试验结果显示:心叶期GAB-3高抗黏虫和亚洲玉米螟,穗期GAB-3高抗亚洲玉米螟。上述结果表明,转基因玉米GAB-3对4种主要玉米鳞翅目害虫有很好的杀虫效果,具有较好的商业化应用前景。