Background:Hypertrophy of the ligamentumflavum(HLF)is a common contributor to spinal stenosis which results in significant neurological impairments.Circular RNA(circRNA)circ_0003609 has been linked to HLF;however,the ex...Background:Hypertrophy of the ligamentumflavum(HLF)is a common contributor to spinal stenosis which results in significant neurological impairments.Circular RNA(circRNA)circ_0003609 has been linked to HLF;however,the exact mechanism by which it causes this disease is unclear.Methods:Circ_0003609 expressions were regulated in HLF cells by overexpression vectors and RNA interference.Cell proliferation andfibrosis-related gene expression were checked by the Cell Counting Kit-8(CCK-8)assay and western blotting.CircBank’s prediction of the association between miR-155 and circ_0003609 was supported by a dual-luciferase reporter experiment.The function of the miR-155/sirtuin 1(SIRT1)axis in controlling HLFfibrosis was further examined.Results:Overexpression of circ_0003609 suppressed HLF cell propagation andfibrosis compared to its silencing.It was found that circ_0003609 served as the sponge for miR-155 and that the circ_0003609/miR-155 axis controlled thefibrosis of HLF cells.It was found that circ_0003609 acted as a sponge for miR-155,regulating thefibrosis of HLF cells.Further,miR-155 targets SIRT1,and the miR-155/SIRT1 axis promotes HLF cellfibrosis.Conclusion:Circ_0003609 ameliorates hypertrophied ligamentumflavum(LF)by modulating the miR-155/SIRT1 axis,indicating a potential treatment approach for HLF.展开更多
BACKGROUND Ligamentum flavum cysts,which are most common in mobile junctional levels of the spine,can be a rare cause of spinal stenosis.There have been several case reports of ligamentum flavum cysts.However,there is...BACKGROUND Ligamentum flavum cysts,which are most common in mobile junctional levels of the spine,can be a rare cause of spinal stenosis.There have been several case reports of ligamentum flavum cysts.However,there is yet to be a documented case report of a calcified ligamentum flavum cyst.Herein,we report the first case of a calcified ligamentum flavum cyst causing ankle and toe weakness.CASE SUMMARY A 66-year-old male visited our hospital complaining of claudication as well as thigh and calf pain in his left leg,all beginning two weeks prior.Physical examination revealed motor weakness of the left ankle dorsiflexion and great toe dorsiflexion.Lumbar spinal computed tomography scans showed spinal stenosis combined with a calcified mass at the left side of the L4-5 level.Magnetic reso-nance imaging showed dural sac compression caused by the calcified mass at the left ligamentum flavum of the L4-5 level.We performed decompressive laminectomy and excision of the calcified mass combined with posterior lumbar interbody fusion at the L4-5 level.Intra-operatively,we found a firm and nodule like mass originating from the ventral surface of ligamentum flavum.Pathological examination suggested a calcified pseudocyst without a capsular lining.After the operation,the patient’s motor weakness in the ankle and great toe improved gradually.CONCLUSION The patient’s ankle and great toe weakness were improved successfully after surgical removal of the calcified cyst.展开更多
背景:既往认为黄韧带骨化在人群中罕见发生,随着研究深入,发现其发病率逐渐上升,这引起大量学者的研究兴趣。目的:通过检索科学引文索引数据库核心集自1999年以来的有关黄韧带骨化的研究成果,使用文献计量学方法对数据进行可视化分析,...背景:既往认为黄韧带骨化在人群中罕见发生,随着研究深入,发现其发病率逐渐上升,这引起大量学者的研究兴趣。目的:通过检索科学引文索引数据库核心集自1999年以来的有关黄韧带骨化的研究成果,使用文献计量学方法对数据进行可视化分析,回顾黄韧带骨化的研究历史,指示重要文献,梳理研究热点,为学者寻找研究方向提供思路。方法:以Web of Science核心集数据库为主,搜索并筛选黄韧带骨化相关论文,使用VOSviewer 1.6.19和CiteSpace 6.2.R6软件对年度发文量、研究国家、机构、被引文献、期刊、作者和关键词等进行可视化分析。结果与结论:①共纳入347篇文献,自1999年发文量呈螺旋上升,中国研究较日本起步晚,但是发文量后来居上,北京大学是发文量最多的机构,北京大学的陈仲强教授是发文量最多的学者。②被引用最多的10篇文献中5篇都与黄韧带骨化的手术治疗有关。③去除与研究主题直接相关的关键词,综合词频和中介中心性的结果发现,“胸椎脊髓病”“硬膜骨化”“微创手术”“后纵韧带骨化”处于领域研究的核心地位。④关键词聚类分析显示,黄韧带骨化的临床表现和手术治疗在疾病的研究中占比较大。⑤关键词时间线图及关键词突现分析显示,“微创手术”作为关键词出现在2015年前后,是突现强度最高的关键词,也是突现开始时间最晚的关键词,2019年开始受到学者广泛关注,关键词“硬膜骨化”的突现尚未结束。⑥黄韧带骨化的手术治疗一直处于研究前沿,微创手术方式的开发和研究、黄韧带骨化继发硬膜骨化的研究是该领域的研究热点。展开更多
目的探讨Delta大通道脊柱内镜下单侧入路双侧减压技术治疗胸椎黄韧带骨化症的临床疗效。方法回顾性分析2019年8月至2022年1月潍坊市人民医院采用Delta大通道脊柱内镜治疗的13例胸椎黄韧带骨化症患者,其中男6例,女7例;年龄55~69岁,平均(6...目的探讨Delta大通道脊柱内镜下单侧入路双侧减压技术治疗胸椎黄韧带骨化症的临床疗效。方法回顾性分析2019年8月至2022年1月潍坊市人民医院采用Delta大通道脊柱内镜治疗的13例胸椎黄韧带骨化症患者,其中男6例,女7例;年龄55~69岁,平均(62.62±4.01)岁。记录患者基本信息及围手术期相关指标,并在术后1个月、6个月进行随访,记录术前术后改良日本骨科协会评分(modified Japanese orthopaedic association score,mJOA)、美国脊髓损伤协会(American spinal injury association,ASIA)感觉评分(ASIA sense score,ASS)和双下肢运动评分(ASIA motor score,AMS)变化情况。以mJOA评分计算术后6个月随访时的改善率(recover rate,RR)及优良率,对RR与性别、年龄、术前mJOA评分、影像学分型及病程进行相关性分析。结果平均手术时间为(108.46±16.12)min,平均出血量(23.46±7.47)mL,平均住院时间(6.08±0.86)d。术后随访时间为6~35个月,平均(16.77±9.33)个月,术后1个月及6个月随访时,mJOA、ASS、AMS评分均优于术前(P<0.05),平均RR为(55.69±19.73)%,优良率为69.23%。RR与术前病程呈负相关(P<0.001),与术前mJOA评分呈正相关(P=0.029),与性别、年龄、影像学分型均无明显相关性(P>0.05)。结论Delta大通道脊柱内镜技术治疗胸椎黄韧带骨化症安全可靠,具有术后恢复快、创伤小、出血量少等优势,短期效果良好。展开更多
目的基于Cite Space探讨腰椎黄韧带有限元模型研究的热点与趋势。方法检索1990年1月至2023年8月中国知网、维普网、万方数据知识服务平台3大数据库中,腰椎黄韧带有限元模型的相关中文文献;运用Cite Space 5.7.R5软件生成作者、机构合作...目的基于Cite Space探讨腰椎黄韧带有限元模型研究的热点与趋势。方法检索1990年1月至2023年8月中国知网、维普网、万方数据知识服务平台3大数据库中,腰椎黄韧带有限元模型的相关中文文献;运用Cite Space 5.7.R5软件生成作者、机构合作、关键词等图谱。结果共纳入文献1395篇,发文量总体呈增长趋势;核心作者丁宇发文最多;发文量最多机构为上海大学期刊社;关键词中“生物力学”出现频次最高;生成了“生物力学”等9个聚类;时间线路图中,最大的节点为“姿势性脊柱后凸”;突现图中“活动度”的研究持续至今。结论腰椎黄韧带有限元模型领域的研究总体增加,主要热点是“生物力学”,“脊柱”将是未来有限元领域的研究重点。展开更多
[ Objective] The paper was to study the contact toxicity and antifeedant activity of Aconitum flavum against cabbage worm. [ Method ] In- sect dipping method was adopted to determine the contact toxicity of the extrac...[ Objective] The paper was to study the contact toxicity and antifeedant activity of Aconitum flavum against cabbage worm. [ Method ] In- sect dipping method was adopted to determine the contact toxicity of the extracts of A. fiavum extracted from five polar solvents including ethanol, petroleum ether, ether, ethyl acetate, n-butanol and water; leaf dish method was adopted to determine the antifeedant activities of five solvent ex- tracts including ethanol, petroleum ether, ether, ethyl acetate, n-butanol and water against cabbage worm, [ Result] Extracts of A. flavum had high contact toxicity against cabbage worm. When the concentration was 100.00 mg/ml, the corrected mortality at 48 h roached 97.24%, and the insec- ticidal activities of five solvent extracts against cabbage worm in sequence were water 〉 n-butanol 〉 ethyl acetate 〉 ether 〉 petroleum ether, the cor- rected mortality of water extract at 48 h was 95.87% ; the antifeedant activities of five solvent extracts in sequence were water 〉 n-butanol 〉 ethyl ac- etate 〉 ether 〉 petroleum ether. [ Conclusion] Extracts of A. flavum had strong contact toxicity and antifeedant activity against cabbage worm, and the active ingredients with contact toxicity and antifeedant activity might be a kind of polar compound.展开更多
[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistr...[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistry were employed for performing the analysis in this study.[Result] The root system of Aconitum flavum Hand.-Mazz.consists of taproot,lateral root and adventitious root.The primary structure of root system is normal,but secondary structure shows abnormal.The cambium and the extra cambium of taproot form a ＂U＂-shaped secondary vascular bundle and tertiary bundle in abnormal secondary structure.The sieve tube group is made of little sieve tube group which is differentiated from primary phloem and cambium.Meanwhile,the secondary xylem in tuberous root also appears to be a ＂U＂ shape.Parenchyma cells of secondary phloem occupy most of the tuberous root.The sieve tube group of tuberous root is mainly differentiated from parenchyma cell of secondary phloem.[Conclusion] The difference in abnormal secondary structure of taproot and tuberous root are attributed to their varied cambium compose and activity pattern.Alkaloids are mainly accumulated in parenchyma cell of the inside cortex and between bundle in taproot,while parenchyma of secondary phloem and pith in tuberous root.展开更多
【目的】探究非特异性腰痛(nonspecific low back pain,NLBP)患者的中医体质类型及其黄韧带厚度与年龄、体质量指数(body mass index,BMI)、性别、中医体质类型、有无糖尿病、高血压分级的相关性。【方法】选取2023年1月~2023年6月广东...【目的】探究非特异性腰痛(nonspecific low back pain,NLBP)患者的中医体质类型及其黄韧带厚度与年龄、体质量指数(body mass index,BMI)、性别、中医体质类型、有无糖尿病、高血压分级的相关性。【方法】选取2023年1月~2023年6月广东省第二中医院收治的60例NLBP患者为研究对象,辨别患者的中医体质类型,测量电子计算机断层扫描(CT)图像腰椎4/5节段(L4/5)椎间盘水平黄韧带的厚度,记录患者的年龄、性别、中医体质类型、BMI、有无糖尿病、高血压分级,通过相关分析、线性回归分析探讨NLBP患者黄韧带厚度的相关影响因素。【结果】(1)60例NLBP患者黄韧带厚度平均为(2.60±0.72)mm。(2)NLBP患者的中医体质呈4类,其中以血瘀质分布最多,达21例(35.0%),其他从高到低依次为湿热质19例(31.7%)、痰湿质12例(20.0%)、气虚质8例(13.3%)。(3)相关分析结果显示:NLBP患者的BMI、性别、中医体质类型、有无糖尿病对黄韧带厚度的影响差异无统计学意义(P>0.05),而不同年龄、高血压分级对黄韧带厚度的影响差异有统计学意义(P<0.01)。(4)线性回归分析结果显示:不同年龄对黄韧带厚度的影响差异有统计学意义(b=0.034,t=6.282,P<0.01),而不同高血压分级对黄韧带厚度的影响差异无统计学意义(P>0.05)。【结论】NLBP患者的中医体质类型以血瘀质为主,其黄韧带厚度受年龄影响较为显著,而高血压可能是潜在的黄韧带厚度的影响因素。展开更多
基金This research was supported by the Shanghai Natural Science Fund(No.21ZR1447500)Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital Baoshan Branch Medical Key Specialty Construction Project(No.rbzdzk-2023-001).
文摘Background:Hypertrophy of the ligamentumflavum(HLF)is a common contributor to spinal stenosis which results in significant neurological impairments.Circular RNA(circRNA)circ_0003609 has been linked to HLF;however,the exact mechanism by which it causes this disease is unclear.Methods:Circ_0003609 expressions were regulated in HLF cells by overexpression vectors and RNA interference.Cell proliferation andfibrosis-related gene expression were checked by the Cell Counting Kit-8(CCK-8)assay and western blotting.CircBank’s prediction of the association between miR-155 and circ_0003609 was supported by a dual-luciferase reporter experiment.The function of the miR-155/sirtuin 1(SIRT1)axis in controlling HLFfibrosis was further examined.Results:Overexpression of circ_0003609 suppressed HLF cell propagation andfibrosis compared to its silencing.It was found that circ_0003609 served as the sponge for miR-155 and that the circ_0003609/miR-155 axis controlled thefibrosis of HLF cells.It was found that circ_0003609 acted as a sponge for miR-155,regulating thefibrosis of HLF cells.Further,miR-155 targets SIRT1,and the miR-155/SIRT1 axis promotes HLF cellfibrosis.Conclusion:Circ_0003609 ameliorates hypertrophied ligamentumflavum(LF)by modulating the miR-155/SIRT1 axis,indicating a potential treatment approach for HLF.
文摘BACKGROUND Ligamentum flavum cysts,which are most common in mobile junctional levels of the spine,can be a rare cause of spinal stenosis.There have been several case reports of ligamentum flavum cysts.However,there is yet to be a documented case report of a calcified ligamentum flavum cyst.Herein,we report the first case of a calcified ligamentum flavum cyst causing ankle and toe weakness.CASE SUMMARY A 66-year-old male visited our hospital complaining of claudication as well as thigh and calf pain in his left leg,all beginning two weeks prior.Physical examination revealed motor weakness of the left ankle dorsiflexion and great toe dorsiflexion.Lumbar spinal computed tomography scans showed spinal stenosis combined with a calcified mass at the left side of the L4-5 level.Magnetic reso-nance imaging showed dural sac compression caused by the calcified mass at the left ligamentum flavum of the L4-5 level.We performed decompressive laminectomy and excision of the calcified mass combined with posterior lumbar interbody fusion at the L4-5 level.Intra-operatively,we found a firm and nodule like mass originating from the ventral surface of ligamentum flavum.Pathological examination suggested a calcified pseudocyst without a capsular lining.After the operation,the patient’s motor weakness in the ankle and great toe improved gradually.CONCLUSION The patient’s ankle and great toe weakness were improved successfully after surgical removal of the calcified cyst.
文摘背景:既往认为黄韧带骨化在人群中罕见发生,随着研究深入,发现其发病率逐渐上升,这引起大量学者的研究兴趣。目的:通过检索科学引文索引数据库核心集自1999年以来的有关黄韧带骨化的研究成果,使用文献计量学方法对数据进行可视化分析,回顾黄韧带骨化的研究历史,指示重要文献,梳理研究热点,为学者寻找研究方向提供思路。方法:以Web of Science核心集数据库为主,搜索并筛选黄韧带骨化相关论文,使用VOSviewer 1.6.19和CiteSpace 6.2.R6软件对年度发文量、研究国家、机构、被引文献、期刊、作者和关键词等进行可视化分析。结果与结论:①共纳入347篇文献,自1999年发文量呈螺旋上升,中国研究较日本起步晚,但是发文量后来居上,北京大学是发文量最多的机构,北京大学的陈仲强教授是发文量最多的学者。②被引用最多的10篇文献中5篇都与黄韧带骨化的手术治疗有关。③去除与研究主题直接相关的关键词,综合词频和中介中心性的结果发现,“胸椎脊髓病”“硬膜骨化”“微创手术”“后纵韧带骨化”处于领域研究的核心地位。④关键词聚类分析显示,黄韧带骨化的临床表现和手术治疗在疾病的研究中占比较大。⑤关键词时间线图及关键词突现分析显示,“微创手术”作为关键词出现在2015年前后,是突现强度最高的关键词,也是突现开始时间最晚的关键词,2019年开始受到学者广泛关注,关键词“硬膜骨化”的突现尚未结束。⑥黄韧带骨化的手术治疗一直处于研究前沿,微创手术方式的开发和研究、黄韧带骨化继发硬膜骨化的研究是该领域的研究热点。
文摘目的探讨Delta大通道脊柱内镜下单侧入路双侧减压技术治疗胸椎黄韧带骨化症的临床疗效。方法回顾性分析2019年8月至2022年1月潍坊市人民医院采用Delta大通道脊柱内镜治疗的13例胸椎黄韧带骨化症患者,其中男6例,女7例;年龄55~69岁,平均(62.62±4.01)岁。记录患者基本信息及围手术期相关指标,并在术后1个月、6个月进行随访,记录术前术后改良日本骨科协会评分(modified Japanese orthopaedic association score,mJOA)、美国脊髓损伤协会(American spinal injury association,ASIA)感觉评分(ASIA sense score,ASS)和双下肢运动评分(ASIA motor score,AMS)变化情况。以mJOA评分计算术后6个月随访时的改善率(recover rate,RR)及优良率,对RR与性别、年龄、术前mJOA评分、影像学分型及病程进行相关性分析。结果平均手术时间为(108.46±16.12)min,平均出血量(23.46±7.47)mL,平均住院时间(6.08±0.86)d。术后随访时间为6~35个月,平均(16.77±9.33)个月,术后1个月及6个月随访时,mJOA、ASS、AMS评分均优于术前(P<0.05),平均RR为(55.69±19.73)%,优良率为69.23%。RR与术前病程呈负相关(P<0.001),与术前mJOA评分呈正相关(P=0.029),与性别、年龄、影像学分型均无明显相关性(P>0.05)。结论Delta大通道脊柱内镜技术治疗胸椎黄韧带骨化症安全可靠,具有术后恢复快、创伤小、出血量少等优势,短期效果良好。
文摘目的基于Cite Space探讨腰椎黄韧带有限元模型研究的热点与趋势。方法检索1990年1月至2023年8月中国知网、维普网、万方数据知识服务平台3大数据库中,腰椎黄韧带有限元模型的相关中文文献;运用Cite Space 5.7.R5软件生成作者、机构合作、关键词等图谱。结果共纳入文献1395篇,发文量总体呈增长趋势;核心作者丁宇发文最多;发文量最多机构为上海大学期刊社;关键词中“生物力学”出现频次最高;生成了“生物力学”等9个聚类;时间线路图中,最大的节点为“姿势性脊柱后凸”;突现图中“活动度”的研究持续至今。结论腰椎黄韧带有限元模型领域的研究总体增加,主要热点是“生物力学”,“脊柱”将是未来有限元领域的研究重点。
基金Supported by Scientific and Technological Projects of Ningxia Hui Autonomous Region(2008220)~~
文摘[ Objective] The paper was to study the contact toxicity and antifeedant activity of Aconitum flavum against cabbage worm. [ Method ] In- sect dipping method was adopted to determine the contact toxicity of the extracts of A. fiavum extracted from five polar solvents including ethanol, petroleum ether, ether, ethyl acetate, n-butanol and water; leaf dish method was adopted to determine the antifeedant activities of five solvent ex- tracts including ethanol, petroleum ether, ether, ethyl acetate, n-butanol and water against cabbage worm, [ Result] Extracts of A. flavum had high contact toxicity against cabbage worm. When the concentration was 100.00 mg/ml, the corrected mortality at 48 h roached 97.24%, and the insec- ticidal activities of five solvent extracts against cabbage worm in sequence were water 〉 n-butanol 〉 ethyl acetate 〉 ether 〉 petroleum ether, the cor- rected mortality of water extract at 48 h was 95.87% ; the antifeedant activities of five solvent extracts in sequence were water 〉 n-butanol 〉 ethyl ac- etate 〉 ether 〉 petroleum ether. [ Conclusion] Extracts of A. flavum had strong contact toxicity and antifeedant activity against cabbage worm, and the active ingredients with contact toxicity and antifeedant activity might be a kind of polar compound.
基金Supported by Natural Science Foundation of Ningxia Hui Autonomous Region(NZ0932)National Key Technology R & D Program of China(20068AI06A15-11)~~
文摘[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistry were employed for performing the analysis in this study.[Result] The root system of Aconitum flavum Hand.-Mazz.consists of taproot,lateral root and adventitious root.The primary structure of root system is normal,but secondary structure shows abnormal.The cambium and the extra cambium of taproot form a ＂U＂-shaped secondary vascular bundle and tertiary bundle in abnormal secondary structure.The sieve tube group is made of little sieve tube group which is differentiated from primary phloem and cambium.Meanwhile,the secondary xylem in tuberous root also appears to be a ＂U＂ shape.Parenchyma cells of secondary phloem occupy most of the tuberous root.The sieve tube group of tuberous root is mainly differentiated from parenchyma cell of secondary phloem.[Conclusion] The difference in abnormal secondary structure of taproot and tuberous root are attributed to their varied cambium compose and activity pattern.Alkaloids are mainly accumulated in parenchyma cell of the inside cortex and between bundle in taproot,while parenchyma of secondary phloem and pith in tuberous root.
文摘【目的】探究非特异性腰痛(nonspecific low back pain,NLBP)患者的中医体质类型及其黄韧带厚度与年龄、体质量指数(body mass index,BMI)、性别、中医体质类型、有无糖尿病、高血压分级的相关性。【方法】选取2023年1月~2023年6月广东省第二中医院收治的60例NLBP患者为研究对象,辨别患者的中医体质类型,测量电子计算机断层扫描(CT)图像腰椎4/5节段(L4/5)椎间盘水平黄韧带的厚度,记录患者的年龄、性别、中医体质类型、BMI、有无糖尿病、高血压分级,通过相关分析、线性回归分析探讨NLBP患者黄韧带厚度的相关影响因素。【结果】(1)60例NLBP患者黄韧带厚度平均为(2.60±0.72)mm。(2)NLBP患者的中医体质呈4类,其中以血瘀质分布最多,达21例(35.0%),其他从高到低依次为湿热质19例(31.7%)、痰湿质12例(20.0%)、气虚质8例(13.3%)。(3)相关分析结果显示:NLBP患者的BMI、性别、中医体质类型、有无糖尿病对黄韧带厚度的影响差异无统计学意义(P>0.05),而不同年龄、高血压分级对黄韧带厚度的影响差异有统计学意义(P<0.01)。(4)线性回归分析结果显示:不同年龄对黄韧带厚度的影响差异有统计学意义(b=0.034,t=6.282,P<0.01),而不同高血压分级对黄韧带厚度的影响差异无统计学意义(P>0.05)。【结论】NLBP患者的中医体质类型以血瘀质为主,其黄韧带厚度受年龄影响较为显著,而高血压可能是潜在的黄韧带厚度的影响因素。
