[Objectives]To observe the effects of polysaccharides from Dicliptera chinensis(L.)Nees.on the expression of TLR/NF-κB pathway related proteins in HepG2 cells induced by oleic acid,and to explore the possible mechani...[Objectives]To observe the effects of polysaccharides from Dicliptera chinensis(L.)Nees.on the expression of TLR/NF-κB pathway related proteins in HepG2 cells induced by oleic acid,and to explore the possible mechanism of polysaccharides from D.chinensis(L.)Nees.in the treatment of non-alcoholic fatty liver disease(NAFLD).[Methods]HepG2 cells were induced with oleic acid to establish a non-alcoholic fatty liver cell model.After intervention with 0.25 and 0.5 mg/mL of D.chinensis(L.)Nees.polysaccharides,the ALT and AST activity and TG and TC contents were detected with kits,and the changes in the expression of CDK5,TLR4,p-NF-κB and NF-κB were analyzed using Western-blotting.[Results]In the HepG2 cells induced with oleic acid,the ALT and AST activity increased significantly,the TG and TC contents increased significantly,and the expression levels of CDK5,TLR4 and p-NF-κB proteins up-regulated significantly.In the HepG2 cells intervened with D.chinensis(L.)Nees.polysaccharides,the activity of ALT and AST,the contents of TG and TC,and the expression levels of CDK5,TLR4 and p-NF-κB proteins all reduced significantly.[Conclusions]Polysaccharides from D.chinensis(L.)Nees.may interfere with NAFLD by inhibiting the TLR4/NF-κB pathway.展开更多
为有效控制福建地区直播稻田禾本科杂草的危害，以莆田市仙游县游洋乡的水稻种植基地为试验点，选择测定1％噁嗪草酮悬浮剂、10％双草醚悬浮剂和20％氰氟草酯油悬浮剂等3种除草剂对稗草、千金子等禾本科杂草的防治效果，筛选确定最佳除...为有效控制福建地区直播稻田禾本科杂草的危害，以莆田市仙游县游洋乡的水稻种植基地为试验点，选择测定1％噁嗪草酮悬浮剂、10％双草醚悬浮剂和20％氰氟草酯油悬浮剂等3种除草剂对稗草、千金子等禾本科杂草的防治效果，筛选确定最佳除草剂及其理想的田间使用浓度。结果表明：20％氰氟草酯油悬浮剂是防治直播稻田中稗草、千金子等禾本科杂草最佳的除草剂，田间推荐使用浓度为45～105 g · hm^ －2（有效成分）兑水675 L · hm ^－2，药后30 d防治效果达均可80.00％以上，无药害产生，对水稻安全。展开更多
基金National Natural Science Foundation of China(81960779,81760114,81660104,81860673)Natural Science Foundation of Guangxi Zhuang Autonomous Region(2017GXNSFAA198218,2017GXNSFAA198326,2018GXNSFAA281040)。
文摘[Objectives]To observe the effects of polysaccharides from Dicliptera chinensis(L.)Nees.on the expression of TLR/NF-κB pathway related proteins in HepG2 cells induced by oleic acid,and to explore the possible mechanism of polysaccharides from D.chinensis(L.)Nees.in the treatment of non-alcoholic fatty liver disease(NAFLD).[Methods]HepG2 cells were induced with oleic acid to establish a non-alcoholic fatty liver cell model.After intervention with 0.25 and 0.5 mg/mL of D.chinensis(L.)Nees.polysaccharides,the ALT and AST activity and TG and TC contents were detected with kits,and the changes in the expression of CDK5,TLR4,p-NF-κB and NF-κB were analyzed using Western-blotting.[Results]In the HepG2 cells induced with oleic acid,the ALT and AST activity increased significantly,the TG and TC contents increased significantly,and the expression levels of CDK5,TLR4 and p-NF-κB proteins up-regulated significantly.In the HepG2 cells intervened with D.chinensis(L.)Nees.polysaccharides,the activity of ALT and AST,the contents of TG and TC,and the expression levels of CDK5,TLR4 and p-NF-κB proteins all reduced significantly.[Conclusions]Polysaccharides from D.chinensis(L.)Nees.may interfere with NAFLD by inhibiting the TLR4/NF-κB pathway.
文摘为有效控制福建地区直播稻田禾本科杂草的危害，以莆田市仙游县游洋乡的水稻种植基地为试验点，选择测定1％噁嗪草酮悬浮剂、10％双草醚悬浮剂和20％氰氟草酯油悬浮剂等3种除草剂对稗草、千金子等禾本科杂草的防治效果，筛选确定最佳除草剂及其理想的田间使用浓度。结果表明：20％氰氟草酯油悬浮剂是防治直播稻田中稗草、千金子等禾本科杂草最佳的除草剂，田间推荐使用浓度为45～105 g · hm^ －2（有效成分）兑水675 L · hm ^－2，药后30 d防治效果达均可80.00％以上，无药害产生，对水稻安全。
