Objective:To detect leptospiral antibodies by microscopic agglutination test(MAT) in northeast of Iran.Methods:This study was conducted to evaluate prevalence of human leptospiral infections by MAT.using six current r...Objective:To detect leptospiral antibodies by microscopic agglutination test(MAT) in northeast of Iran.Methods:This study was conducted to evaluate prevalence of human leptospiral infections by MAT.using six current reference strains of Leptospira interrogans in north-east of Iran.A total of 285 serum samples were collected from three north-east provinces of Iran,from December,2009 to June,2010.Results:Antibodies were detected at least against one serovar of Leptospira interrogans in 45 sera(15.79%) among 283 samples at a dilution 1:100 or greater.Positive titers against more than one serovar were detected in 24 sera of the positive samples.Therefore, there were 75 positive reactions against different serovar of Leptospira interrogans.Positive titers were recorded against serovar icterohaemorrhagiae(31 samples),hardjo(26 samples), grippotyphosa(7 samples),pomona(5 samples),canicola(4 samples) and ballum(2.sample). Conclusions:In present study the most prevalent(Leptospira icterohaemorrhagiae) and the least prevalent(Leptospira ballum) serovar are different from previous studies.Maybe,species and prevalence of serovars change during the lime in one area and between regions.展开更多
Objective:To evaluate the prevalence and divergence of genetically identified Leptospira spp.in the population of Rattus rattus.Methods:A total of 130 rats were used in this study.The infection within the rats were sc...Objective:To evaluate the prevalence and divergence of genetically identified Leptospira spp.in the population of Rattus rattus.Methods:A total of 130 rats were used in this study.The infection within the rats were screened using polymerase chain reaction(PCR)-based diagnosis,with Leptospira genusspecific 16 S r RNA primer and pathogenic Leptospira spp.specific Lip L32 primer,on both kidney and liver tissues of Rattus rattus to detect the presence of potential Leptospira spp.Results:Out of 130 rats studied,51(39.23%)individuals were positive for leptospiral DNA.Basic Local Alignment Search Tool(BLAST)and phylogenetic analysis revealed that both pathogenic Leptospira interrogans and Leptospira borgpetersenii were predominantly identified.Phylogenetically,both genes disclosed similar clustering patterns of tree topologies between the two species.Although both genes were conserved,Lip L32 gene portrayed higher nucleotide divergence(5.80%)compared to the 16 S r RNA gene(0.60%).Minimum-spanning network displayed several haplotypes that are unique to each species,suggesting a higher degree of subdivision between both species.As for prevalence surveillance,both adult and subadult rats were susceptible to the infection,in which males were the most susceptible.Kidney was notable as the favourable organ for colonisation of leptospires.Rats captured from fresh markets were highly infected with Leptospira spp.(54.28%)compared to those from housing areas(26.47%).Conclusions:Rattus rattus represents an important asymptomatic transmitter of pathogenic leptospires,and hence is of public health concerns.展开更多
基金Supported by a grant form Research Council of Shahid BahonarUniversity of Kerman.Iran
文摘Objective:To detect leptospiral antibodies by microscopic agglutination test(MAT) in northeast of Iran.Methods:This study was conducted to evaluate prevalence of human leptospiral infections by MAT.using six current reference strains of Leptospira interrogans in north-east of Iran.A total of 285 serum samples were collected from three north-east provinces of Iran,from December,2009 to June,2010.Results:Antibodies were detected at least against one serovar of Leptospira interrogans in 45 sera(15.79%) among 283 samples at a dilution 1:100 or greater.Positive titers against more than one serovar were detected in 24 sera of the positive samples.Therefore, there were 75 positive reactions against different serovar of Leptospira interrogans.Positive titers were recorded against serovar icterohaemorrhagiae(31 samples),hardjo(26 samples), grippotyphosa(7 samples),pomona(5 samples),canicola(4 samples) and ballum(2.sample). Conclusions:In present study the most prevalent(Leptospira icterohaemorrhagiae) and the least prevalent(Leptospira ballum) serovar are different from previous studies.Maybe,species and prevalence of serovars change during the lime in one area and between regions.
文摘Objective:To evaluate the prevalence and divergence of genetically identified Leptospira spp.in the population of Rattus rattus.Methods:A total of 130 rats were used in this study.The infection within the rats were screened using polymerase chain reaction(PCR)-based diagnosis,with Leptospira genusspecific 16 S r RNA primer and pathogenic Leptospira spp.specific Lip L32 primer,on both kidney and liver tissues of Rattus rattus to detect the presence of potential Leptospira spp.Results:Out of 130 rats studied,51(39.23%)individuals were positive for leptospiral DNA.Basic Local Alignment Search Tool(BLAST)and phylogenetic analysis revealed that both pathogenic Leptospira interrogans and Leptospira borgpetersenii were predominantly identified.Phylogenetically,both genes disclosed similar clustering patterns of tree topologies between the two species.Although both genes were conserved,Lip L32 gene portrayed higher nucleotide divergence(5.80%)compared to the 16 S r RNA gene(0.60%).Minimum-spanning network displayed several haplotypes that are unique to each species,suggesting a higher degree of subdivision between both species.As for prevalence surveillance,both adult and subadult rats were susceptible to the infection,in which males were the most susceptible.Kidney was notable as the favourable organ for colonisation of leptospires.Rats captured from fresh markets were highly infected with Leptospira spp.(54.28%)compared to those from housing areas(26.47%).Conclusions:Rattus rattus represents an important asymptomatic transmitter of pathogenic leptospires,and hence is of public health concerns.
