Leucine rich repeat(LRR)domain,characterized by a repetitive sequence pattern rich in leucine residues,is a universal protein-protein interaction motif present in all life forms.LRR repeats interrupted by sequences of...Leucine rich repeat(LRR)domain,characterized by a repetitive sequence pattern rich in leucine residues,is a universal protein-protein interaction motif present in all life forms.LRR repeats interrupted by sequences of 30 70 residues(termed island domain,ID)have been found in some plant LRR receptor-like kinases(RLKs)and animal Toll-like receptors(TLR7-9).Recent studies provide insight into how a single ID is structurally integrated into an LRR protein.However,structural information on an LRR protein with two IDs is lacking.The receptor-like protein kinase 2(RPK2)is an LRR-RLK and has important roles in controlling plant growth and development by perception and transduction of hormone signal.Here we present the crystal structure of the extracellular LRR domain of RPK2(RPK2-LRR)containing two IDs from Arabidopsis.The structure reveals that both of the IDs are helical and located at the central region of the single RPK2-LRR solenoid.One of them binds to the inner surface of the solenoid,whereas the other one mainly interacts with the lateral side.Unexpectedly,a long loop immediately following the N-terminal capping domain of RPK2-LRR is presented toward and sandwiched between the two IDs,further stabilizing their embedding to the LRR solenoid.A potential ligand binding site formed by the two IDs and the solenoid is located at the C-terminal side of RPK2-LRR.The structural information of RPK2-LRR broadens our understanding toward the large family of LRR proteins and provides insight into RPK2-mediated signaling.展开更多
Two sets of degenerate oligonucleotide primers were designed according to amino acid conserved regions of reported plant disease resistance genes which encode proteins that contain nucleotide-binding site and leucine-...Two sets of degenerate oligonucleotide primers were designed according to amino acid conserved regions of reported plant disease resistance genes which encode proteins that contain nucleotide-binding site and leucine-rich repeats(NBS-LRR),and the plant disease resistance genes which encode serine/threonine protein kinase(STK).By polymerase chain reaction(PCR),disease resistance gene analogues have been amplified from three wild rice species in Yunnan Province,China.The DNA fragments from amplification have been cloned into the pGEM-T vector respectively.Sequencing of the DNA fragments indicated that 7 classes,2 classes and 6 classes NBS-LRR disease resistance gene analogues from Oryza rufipogon Griff.Oryza officinalis Wall.and Oryza meyeriana Baill.were obtained respectively.The two representative fragments of TO12 from Ory-za officinalis Wall.and TR19 from Oryza rufipogon Griff.belong to the same class and homology of their sequences are 100%.The result shows that the sequences of the same class disease resistance gene analogues have no difference among different species of wild rice.5 classes STK disease resistance gene analogues were also obtained among which 4 classes from Oryza rufipogon Griff.1 class from Oryza officinalis Wall.By comparison analysis of amino acid sequences,we found that the obtained disease resistance gene analogues have very low identity(low to 25%) with the reported disease resistance gene L6,N,Bs2,Prf,Pto,Lr10 and Xa21 etc.The finding suggests that the obtained disease resistance gene analogues are analogues of putative disease resistance genes that have not been isolated so far.展开更多
基金supported by the National Natural Science Foundation of China(31130063)the National Outstanding Young Scholar Science Foundation of China(31025008)to Chai JiJie
文摘Leucine rich repeat(LRR)domain,characterized by a repetitive sequence pattern rich in leucine residues,is a universal protein-protein interaction motif present in all life forms.LRR repeats interrupted by sequences of 30 70 residues(termed island domain,ID)have been found in some plant LRR receptor-like kinases(RLKs)and animal Toll-like receptors(TLR7-9).Recent studies provide insight into how a single ID is structurally integrated into an LRR protein.However,structural information on an LRR protein with two IDs is lacking.The receptor-like protein kinase 2(RPK2)is an LRR-RLK and has important roles in controlling plant growth and development by perception and transduction of hormone signal.Here we present the crystal structure of the extracellular LRR domain of RPK2(RPK2-LRR)containing two IDs from Arabidopsis.The structure reveals that both of the IDs are helical and located at the central region of the single RPK2-LRR solenoid.One of them binds to the inner surface of the solenoid,whereas the other one mainly interacts with the lateral side.Unexpectedly,a long loop immediately following the N-terminal capping domain of RPK2-LRR is presented toward and sandwiched between the two IDs,further stabilizing their embedding to the LRR solenoid.A potential ligand binding site formed by the two IDs and the solenoid is located at the C-terminal side of RPK2-LRR.The structural information of RPK2-LRR broadens our understanding toward the large family of LRR proteins and provides insight into RPK2-mediated signaling.
文摘Two sets of degenerate oligonucleotide primers were designed according to amino acid conserved regions of reported plant disease resistance genes which encode proteins that contain nucleotide-binding site and leucine-rich repeats(NBS-LRR),and the plant disease resistance genes which encode serine/threonine protein kinase(STK).By polymerase chain reaction(PCR),disease resistance gene analogues have been amplified from three wild rice species in Yunnan Province,China.The DNA fragments from amplification have been cloned into the pGEM-T vector respectively.Sequencing of the DNA fragments indicated that 7 classes,2 classes and 6 classes NBS-LRR disease resistance gene analogues from Oryza rufipogon Griff.Oryza officinalis Wall.and Oryza meyeriana Baill.were obtained respectively.The two representative fragments of TO12 from Ory-za officinalis Wall.and TR19 from Oryza rufipogon Griff.belong to the same class and homology of their sequences are 100%.The result shows that the sequences of the same class disease resistance gene analogues have no difference among different species of wild rice.5 classes STK disease resistance gene analogues were also obtained among which 4 classes from Oryza rufipogon Griff.1 class from Oryza officinalis Wall.By comparison analysis of amino acid sequences,we found that the obtained disease resistance gene analogues have very low identity(low to 25%) with the reported disease resistance gene L6,N,Bs2,Prf,Pto,Lr10 and Xa21 etc.The finding suggests that the obtained disease resistance gene analogues are analogues of putative disease resistance genes that have not been isolated so far.
