DIFFERENTIAL EXPRESSION OF GENES INVOLVED IN METABOLISM BETWEEN TUMORIGENITIC HUMAN LEUKEMIA CELL LINES K562 AND K562-n

Objective: To study the molecular mechanism ofdifferent tumorigenicity in nude mice of human leukemiacell lines K562-n and K562. Methods: To analyze the genes differently expressed between K562 and K562-n cells byusing cDNA microarray technique. Results: Among the12800 genes detected, some genes involved in materialmetabolism and material transport were differentlyexpressed between K562-n and K562 cells. These genesinclude homo sapiens placenta-specific ATP-binding cassette transporter gene, dihydrodiol dehydrogenase gene, hepatic dihydrodiol dehydrogenase gene, NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase,lysophosphatidic acid acyltransferase, alpha gene,argininosuccinate lyase gene, mitochondrial isocitrtatedehydrogenase, adhesion protein SQM1 gene, dimethylarginine dimethylamino-hydrolase gene, M1subunit of ribonucleotide reductase and farnesylpyrophosphate synthetase gene. Conclusion: The hightumorigenicity of K562-n cells is related to the differentexpression of some genes concerned with cell metabolismand material transpoert.

裸鼠高致瘤性K562-n细胞系代谢相关基因表达变化的研究

目的 探索K5 6 2和K5 6 2 n细胞在裸鼠体内致瘤性不同的分子生物学机制。方法 应用基因芯片技术 ,检测K5 6 2细胞和K5 6 2 n细胞的差异表达基因。结果 在被检测的 12 80 0个基因中 ,一些与细胞物质代谢和物质转运有关基因的表达在高致瘤性K5 6 2 n细胞与K5 6 2细胞之间有显著的变化。K5 6 2 n细胞表达上调的基因包括Dihydrodiol脱氢酶基因、肝Dihy drodiol脱氢酶基因、NAD依赖的亚甲基四氢叶酸脱氢酶 /环化脱水酶基因、人胎盘特异性ATP结合盒转运蛋白基因 ,表达下调的基因包括溶血磷脂酸酰基转移酶基因、线粒体异柠檬酸脱氢酶基因、精氨琥珀酸裂解酶基因、核糖核苷酸还原酶M1亚基基因、焦磷酸法尼酯合成酶基因、二甲基精氨酸二甲基氨基水解酶基因、细胞粘附蛋白SQM1基因等。结论 K5 6 2 n细胞的裸鼠高致瘤性等生物学特性与一系列细胞物质代谢酶和物质转运蛋白基因的表达变化有关。