<strong>Objective:</strong> In Japan, red blood cell (RBC) solution is usually transported by car from a medical institution to the patient’s house for home transfusion. However, there are no regulations ...<strong>Objective:</strong> In Japan, red blood cell (RBC) solution is usually transported by car from a medical institution to the patient’s house for home transfusion. However, there are no regulations for transporting blood by car in the medical setting. Therefore, we assessed and compared the methods (containers) used for transporting the RBC solution by car. <strong>Materials and Methods:</strong> Irradiated RBC solution samples (280 mL) supplied by the Japan Red Cross Society were each divided into two bags. The quality of blood transported by car (1 - 2 hours) in an active transport refrigerator (ATR) (control group) was compared with that transported in a cooler, or styrofoam box (study group). We tested the hemolytic effects of transportation by car, storage, and filtration through a transfusion set on the lactate dehydrogenase (LD) levels in the RBC solutions. <strong>Results:</strong> Post-filtered LD levels were significantly higher in the RBC solutions transported in a cooler-box with inadequate temperature control when compared to those transported in an ATR with optimal temperature control. However, under conditions of optimal temperature control, the post-filtered LD levels were comparable in the control and study (both cooler and styrofoam boxes) group RBC solutions. <strong>Conclusion:</strong> Temperature management is critical for the maintenance of the quality of the RBC solution transported by car.展开更多
In this paper, we deal with a model for the survival of red blood cells with periodic coefficients x＇（g）=-μ（t）x（t）＋P（t）e^-γ（t）x（t-τ（t））,t≥0.（＊）A new sufficient condition for global attractivity ...In this paper, we deal with a model for the survival of red blood cells with periodic coefficients x＇（g）=-μ（t）x（t）＋P（t）e^-γ（t）x（t-τ（t））,t≥0.（＊）A new sufficient condition for global attractivity of positive periodic solutions of Eq. （＊） is obtained. Our criterion improves corresponding result obtained by Li and Wang in 2005.展开更多
In this paper,we are concerned with a class of fractional-order Lasota-Wazewska red blood ccll modcls.By applying a fixed point theorem on a normal cone,we first obtain the sufficient conditions for the existence of a...In this paper,we are concerned with a class of fractional-order Lasota-Wazewska red blood ccll modcls.By applying a fixed point theorem on a normal cone,we first obtain the sufficient conditions for the existence of a unique almost periodic positive solution of the considered models.Then,considering that all of the red blood cells in animals survive in a finite-time interval,we study the finite-time stability of the almost periodic positive solution by using some inequality techniques.Our results and methods of this paper are new.Finally,we give numerical examples to show the feasibility of the obtained results.展开更多
目的比较添加不同比例的MAP保存液对西藏拉萨地区悬浮红细胞的保存质量的影响。方法将西藏自治区血液中心采集的400 m L全血(共6袋),每袋平均分成2份,每份再分别离心分离去除血浆,按全血与保存液的不同比例包括4∶1和4∶1.5,每份红细胞...目的比较添加不同比例的MAP保存液对西藏拉萨地区悬浮红细胞的保存质量的影响。方法将西藏自治区血液中心采集的400 m L全血(共6袋),每袋平均分成2份,每份再分别离心分离去除血浆,按全血与保存液的不同比例包括4∶1和4∶1.5,每份红细胞分别加入50 m L(对照组)和75m L MAP保存液(实验组)制备得到悬浮红细胞,每份分别分装到4个50 m L小袋,于1、7、14、35 d分别检测血常规、p H值、全血粘度、电解质、葡萄糖、乳酸、游离Hb、红细胞渗透脆性、红细胞变形性等指标。结果在保存d1,实验组的红细胞数、Hb浓度、Hct、血液粘度都明显低于对照组,分别为(5.66±0.49)vs(6.72±0.68)×1012/L,(178.2±13.03)vs(212.6±14.2)g/L,(63.7±5.9)%vs(53.7±4.7)%,(6.99±1.82)vs(11.60±2.86)10s-1,至保存末期,两组仍保持这一规律。尽管对照组与实验组的红细胞变形指数和渗透脆性在保存期间差异不大,但在保存的d1和d7,对照组的溶血率仍明显高于实验组,分别为(0.070±0.039)vs(0.045±0.039)%和(0.116±0.066)vs(0.070±0.055)%。另外,在排除红细胞浓度的影响后,保存过程中对照组的葡萄糖的代谢速率仍明显快于实验组,为(2.43±0.66)vs(1.93±0.41)mmo L/L/1012个红细胞。结论 2组悬浮红细胞相比,添加正常比例1.5倍的MAP制备的西藏拉萨高海拔的悬浮红细胞的红细胞数、Hb浓度、Hct和血液粘度都明显降低,有利于临床输注。除此之外,红细胞保存液的增加有利于减缓葡萄糖的消耗和减少溶血,对高海拔地区悬浮红细胞的保存质量有一定的改善作用。展开更多
目的探究在冰冻红细胞解冻洗涤液中添加适宜浓度的木糖醇,提高解冻红细胞回收率的可行性。方法选择6 d内制备的400 ml去白细胞悬浮红细胞20袋,离心去浆后分装等量(100 ml)浓缩红细胞作为实验组和对照组,实验组在解冻第1次洗涤液(80 ml 9...目的探究在冰冻红细胞解冻洗涤液中添加适宜浓度的木糖醇,提高解冻红细胞回收率的可行性。方法选择6 d内制备的400 ml去白细胞悬浮红细胞20袋,离心去浆后分装等量(100 ml)浓缩红细胞作为实验组和对照组,实验组在解冻第1次洗涤液(80 ml 9%Na Cl)和第2次洗涤液(200 ml 0.9%Na Cl)中分别加入终浓度为1.2%、0.2%的木糖醇,比较两组的RBC回收率、红细胞渗透脆性、甘油残留量,同时测定冰冻红细胞解冻洗涤过程中各阶段的游离血红蛋白含量,明确提高解冻红细胞回收率的关键步骤。结果实验组RBC回收率显著高于对照组(t=-10.222,P<0.05),而红细胞渗透脆性、甘油残留量与对照组相比差异无统计学意义(P>0.05)。解冻第1次洗涤后血浆游离血红蛋白含量以实验组显著低于对照组(t=24.496,P<0.05),其余洗涤步骤的差异则无统计学意义(P>0.05)。结论冰冻红细胞经第1次洗涤液(80 ml 9%Na Cl)的解冻洗涤过程为RBC回收率提高的关键步骤。展开更多
目的:研究红细胞(red blood cell,RBC)裂解液对CD34^+细胞相对计数的影响。方法:收集101份外周血干细胞采集物,CD34-PE及CD45-FITC双色免疫荧光染色后,分别用FACS裂解液(FACSTM lysing solution,FACS)、Optilyse C裂解液(optilyse C lys...目的:研究红细胞(red blood cell,RBC)裂解液对CD34^+细胞相对计数的影响。方法:收集101份外周血干细胞采集物,CD34-PE及CD45-FITC双色免疫荧光染色后,分别用FACS裂解液(FACSTM lysing solution,FACS)、Optilyse C裂解液(optilyse C lysing solution,Optilyse C)及自配裂解液溶解红细胞,采用洗涤程序和运用ISHAGE设门策略,检测CD34^+细胞、淋巴、粒、单核细胞相对数,CD45^+细胞占全部收集信号百分比及各类细胞前向散射光(forward scatter,FSC)及侧向散射光(side scatter,SSC)信号。结果:比较3种裂解液处理后样本的CD34^+细胞百分比,FACS裂解液处理的样本CD34^+细胞百分比最低,自配裂解液最高(FACS vs. Optilyse C vs.自配=0.49±0.43 vs. 0.67±0.50 vs. 0.73±0.66,P=0.000)。FACS裂解后样本CD45+细胞百分比远高于Optilyse C及自配裂解液(t=142.500,P=0.000)。3种裂解液对淋巴细胞百分比结果(F=35.340,P=0.000)具有统计学差异;而粒细胞百分比(F=0.610,P=0.540)、单核细胞百分比(F=1.590,P=0.210)差异无统计学意义。FACS裂解液处理后,CD34^+细胞、淋巴细胞的FSC和SSC信号强度均弱于Optilyse C裂解液及自配的裂解液;而粒、单核细胞的SSC信号,FACS裂解液处理后最强,Optilyse C裂解液处理后最弱。另外,动员后的白细胞数多少与CD34^+细胞百分比不存在相关性(r=0.108,P=0.312)。结论:红细胞裂解液导致CD34^+细胞相对计数差异的原因可能与CD45^+细胞比例有关,即获取的有效细胞数;裂解液对细胞形态的影响与造成淋巴细胞比例有显著性差异有关。鉴于不同红细胞裂解液对CD34等抗原阳性细胞的相对计数确实有不良影响,因此在应用流式细胞术检测或计数时应慎重选择。展开更多
文摘<strong>Objective:</strong> In Japan, red blood cell (RBC) solution is usually transported by car from a medical institution to the patient’s house for home transfusion. However, there are no regulations for transporting blood by car in the medical setting. Therefore, we assessed and compared the methods (containers) used for transporting the RBC solution by car. <strong>Materials and Methods:</strong> Irradiated RBC solution samples (280 mL) supplied by the Japan Red Cross Society were each divided into two bags. The quality of blood transported by car (1 - 2 hours) in an active transport refrigerator (ATR) (control group) was compared with that transported in a cooler, or styrofoam box (study group). We tested the hemolytic effects of transportation by car, storage, and filtration through a transfusion set on the lactate dehydrogenase (LD) levels in the RBC solutions. <strong>Results:</strong> Post-filtered LD levels were significantly higher in the RBC solutions transported in a cooler-box with inadequate temperature control when compared to those transported in an ATR with optimal temperature control. However, under conditions of optimal temperature control, the post-filtered LD levels were comparable in the control and study (both cooler and styrofoam boxes) group RBC solutions. <strong>Conclusion:</strong> Temperature management is critical for the maintenance of the quality of the RBC solution transported by car.
基金the National Natural Science Foundation of China(No.10271044)Research Fund of Hunan Provincial Education Department(No.06C719)
文摘In this paper, we deal with a model for the survival of red blood cells with periodic coefficients x＇（g）=-μ（t）x（t）＋P（t）e^-γ（t）x（t-τ（t））,t≥0.（＊）A new sufficient condition for global attractivity of positive periodic solutions of Eq. （＊） is obtained. Our criterion improves corresponding result obtained by Li and Wang in 2005.
基金the National Natural Sciences Foundation of People's Republic of China under Grants Nos.11861072 and 11361072the Applied Basic Research Programs of Science and Technology Department of Yunnan Province under Grant No.2019FBO03.
文摘In this paper,we are concerned with a class of fractional-order Lasota-Wazewska red blood ccll modcls.By applying a fixed point theorem on a normal cone,we first obtain the sufficient conditions for the existence of a unique almost periodic positive solution of the considered models.Then,considering that all of the red blood cells in animals survive in a finite-time interval,we study the finite-time stability of the almost periodic positive solution by using some inequality techniques.Our results and methods of this paper are new.Finally,we give numerical examples to show the feasibility of the obtained results.
文摘目的比较添加不同比例的MAP保存液对西藏拉萨地区悬浮红细胞的保存质量的影响。方法将西藏自治区血液中心采集的400 m L全血(共6袋),每袋平均分成2份,每份再分别离心分离去除血浆,按全血与保存液的不同比例包括4∶1和4∶1.5,每份红细胞分别加入50 m L(对照组)和75m L MAP保存液(实验组)制备得到悬浮红细胞,每份分别分装到4个50 m L小袋,于1、7、14、35 d分别检测血常规、p H值、全血粘度、电解质、葡萄糖、乳酸、游离Hb、红细胞渗透脆性、红细胞变形性等指标。结果在保存d1,实验组的红细胞数、Hb浓度、Hct、血液粘度都明显低于对照组,分别为(5.66±0.49)vs(6.72±0.68)×1012/L,(178.2±13.03)vs(212.6±14.2)g/L,(63.7±5.9)%vs(53.7±4.7)%,(6.99±1.82)vs(11.60±2.86)10s-1,至保存末期,两组仍保持这一规律。尽管对照组与实验组的红细胞变形指数和渗透脆性在保存期间差异不大,但在保存的d1和d7,对照组的溶血率仍明显高于实验组,分别为(0.070±0.039)vs(0.045±0.039)%和(0.116±0.066)vs(0.070±0.055)%。另外,在排除红细胞浓度的影响后,保存过程中对照组的葡萄糖的代谢速率仍明显快于实验组,为(2.43±0.66)vs(1.93±0.41)mmo L/L/1012个红细胞。结论 2组悬浮红细胞相比,添加正常比例1.5倍的MAP制备的西藏拉萨高海拔的悬浮红细胞的红细胞数、Hb浓度、Hct和血液粘度都明显降低,有利于临床输注。除此之外,红细胞保存液的增加有利于减缓葡萄糖的消耗和减少溶血,对高海拔地区悬浮红细胞的保存质量有一定的改善作用。
文摘目的探究在冰冻红细胞解冻洗涤液中添加适宜浓度的木糖醇,提高解冻红细胞回收率的可行性。方法选择6 d内制备的400 ml去白细胞悬浮红细胞20袋,离心去浆后分装等量(100 ml)浓缩红细胞作为实验组和对照组,实验组在解冻第1次洗涤液(80 ml 9%Na Cl)和第2次洗涤液(200 ml 0.9%Na Cl)中分别加入终浓度为1.2%、0.2%的木糖醇,比较两组的RBC回收率、红细胞渗透脆性、甘油残留量,同时测定冰冻红细胞解冻洗涤过程中各阶段的游离血红蛋白含量,明确提高解冻红细胞回收率的关键步骤。结果实验组RBC回收率显著高于对照组(t=-10.222,P<0.05),而红细胞渗透脆性、甘油残留量与对照组相比差异无统计学意义(P>0.05)。解冻第1次洗涤后血浆游离血红蛋白含量以实验组显著低于对照组(t=24.496,P<0.05),其余洗涤步骤的差异则无统计学意义(P>0.05)。结论冰冻红细胞经第1次洗涤液(80 ml 9%Na Cl)的解冻洗涤过程为RBC回收率提高的关键步骤。
文摘目的:研究红细胞(red blood cell,RBC)裂解液对CD34^+细胞相对计数的影响。方法:收集101份外周血干细胞采集物,CD34-PE及CD45-FITC双色免疫荧光染色后,分别用FACS裂解液(FACSTM lysing solution,FACS)、Optilyse C裂解液(optilyse C lysing solution,Optilyse C)及自配裂解液溶解红细胞,采用洗涤程序和运用ISHAGE设门策略,检测CD34^+细胞、淋巴、粒、单核细胞相对数,CD45^+细胞占全部收集信号百分比及各类细胞前向散射光(forward scatter,FSC)及侧向散射光(side scatter,SSC)信号。结果:比较3种裂解液处理后样本的CD34^+细胞百分比,FACS裂解液处理的样本CD34^+细胞百分比最低,自配裂解液最高(FACS vs. Optilyse C vs.自配=0.49±0.43 vs. 0.67±0.50 vs. 0.73±0.66,P=0.000)。FACS裂解后样本CD45+细胞百分比远高于Optilyse C及自配裂解液(t=142.500,P=0.000)。3种裂解液对淋巴细胞百分比结果(F=35.340,P=0.000)具有统计学差异;而粒细胞百分比(F=0.610,P=0.540)、单核细胞百分比(F=1.590,P=0.210)差异无统计学意义。FACS裂解液处理后,CD34^+细胞、淋巴细胞的FSC和SSC信号强度均弱于Optilyse C裂解液及自配的裂解液;而粒、单核细胞的SSC信号,FACS裂解液处理后最强,Optilyse C裂解液处理后最弱。另外,动员后的白细胞数多少与CD34^+细胞百分比不存在相关性(r=0.108,P=0.312)。结论:红细胞裂解液导致CD34^+细胞相对计数差异的原因可能与CD45^+细胞比例有关,即获取的有效细胞数;裂解液对细胞形态的影响与造成淋巴细胞比例有显著性差异有关。鉴于不同红细胞裂解液对CD34等抗原阳性细胞的相对计数确实有不良影响,因此在应用流式细胞术检测或计数时应慎重选择。
