BL10U2 is an undulator-based macromolecular crystallography(MX)beamline located at the 3.5-GeV Shanghai Synchrotron Radiation Facility.BL10U2 is specifically designed for conducting routine and biosafety level-2(BSL-2...BL10U2 is an undulator-based macromolecular crystallography(MX)beamline located at the 3.5-GeV Shanghai Synchrotron Radiation Facility.BL10U2 is specifically designed for conducting routine and biosafety level-2(BSL-2)MX experiments utilizing high-flux tunable X-rays with energies from 7 to 18 keV,providing a beam spot size of 20μm(horizontal)×10μm(vertical)at the sample point.Certification by the Shanghai Pudong Municipal Health Commission confirmed the capability to perform BSL-2 MX experiments.The beamline is currently equipped with an Eiger X 16 M detector and two newly developed in-house high-precision diffractometers that can be switched to perform conventional or in situ crystal diffraction experiments.An automatic sample changer developed in-house allows fast sample exchange in less than 30 s,supporting high-throughput MX experimentation and rapid crystal screening.Data collection from both the diffractometer and detector was controlled by an in-house developed data collection software(Finback)with a user-friendly interface for convenient operation.This study presents a comprehensive overview of the facilities,experimental methods,and performance characteristics of the BL10U2 beamline.展开更多
In this pape,we give four methods of discriminations its nonsingularity by utilizing only parametr r1,r2 and elements of the first row of level-2 (r1, r2)-circulant matrices of type (m,n).
The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is still epidemic around the world.The manipulation of SARS-CoV-2 is restricted to biosafety level 3 laboratories(BSL-3).In this study,we developed a SARS...The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is still epidemic around the world.The manipulation of SARS-CoV-2 is restricted to biosafety level 3 laboratories(BSL-3).In this study,we developed a SARS-CoV-2ΔN-GFP-HiBiT replicon delivery particles(RDPs)encoding a dual reporter gene,GFP-HiBiT,capable of producing both GFP signal and luciferase activities.Through optimal selection of the reporter gene,GFP-HiBiT demonstrated superior stability and convenience for antiviral evaluation.Additionally,we established a RDP infection mouse model by delivering the N gene into K18-hACE2 KI mouse through lentivirus.This mouse model supports RDP replication and can be utilized for in vivo antiviral evaluations.In summary,the RDP system serves as a valuable tool for efficient antiviral screening and studying the gene function of SARS-CoV-2.Importantly,this system can be manipulated in BSL-2 laboratories,decreasing the threshold of experimental requirements.展开更多
A significant number of biosafety level 2(BSL-2)laboratories have been established in many countries for studies of various types of pathogenic agents and other infectious biological materials.The harmonized managemen...A significant number of biosafety level 2(BSL-2)laboratories have been established in many countries for studies of various types of pathogenic agents and other infectious biological materials.The harmonized management of biological risks in such diverse laboratories thus appears as a real challenge.Zhejiang Province in China has taken the initiative to establish a comprehensively integrated laboratory biosafety management system called SINS(Standardization,Informatization,Normalization and Systematization).The SINS model system has been introduced and adopted in 1,721 BSL-2 laboratories in Zhejiang Province,and thus lead to an increase in the number of biosafety committees from 20%to more than 95%from 2007 to 2018,and the number of biosafety laboratory managers who knows biosafety-related laws and regulations increase from 52.7%to 83.7%from 2009 to 2017.Such achievements indicate that the successful implementation of SINS model has increased the effective control of biological risks in BSL-2 laboratories of the Zhejiang Province.SINS model and its main effects on leading the improvement of laboratory biosafety management was presented in this review.The SINS model helps to strengthen laboratory biosafety and thus effectively reduces occurrence of biosafety-related incidences.This model can potentially be used by other regions or countries where harmonized biosafety management system is still under-developing.展开更多
基金supported by the SSRF Phase-II projectNatural Science Foundation of Shanghai(Nos.21ZR1471800 and 23ZR1471200)National Natural Science Foundation of China(No.12005281)。
文摘BL10U2 is an undulator-based macromolecular crystallography(MX)beamline located at the 3.5-GeV Shanghai Synchrotron Radiation Facility.BL10U2 is specifically designed for conducting routine and biosafety level-2(BSL-2)MX experiments utilizing high-flux tunable X-rays with energies from 7 to 18 keV,providing a beam spot size of 20μm(horizontal)×10μm(vertical)at the sample point.Certification by the Shanghai Pudong Municipal Health Commission confirmed the capability to perform BSL-2 MX experiments.The beamline is currently equipped with an Eiger X 16 M detector and two newly developed in-house high-precision diffractometers that can be switched to perform conventional or in situ crystal diffraction experiments.An automatic sample changer developed in-house allows fast sample exchange in less than 30 s,supporting high-throughput MX experimentation and rapid crystal screening.Data collection from both the diffractometer and detector was controlled by an in-house developed data collection software(Finback)with a user-friendly interface for convenient operation.This study presents a comprehensive overview of the facilities,experimental methods,and performance characteristics of the BL10U2 beamline.
文摘In this pape,we give four methods of discriminations its nonsingularity by utilizing only parametr r1,r2 and elements of the first row of level-2 (r1, r2)-circulant matrices of type (m,n).
基金supported by grants from the National Key R&D Program of China(2021YFA1300801 and 2018YFA0900801)National Natural Science Foundation of China(82172243 and 82372223)Fundamental Research Funds for the Central Universities(2042021kf0220 and 2042022dx0003).
文摘The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is still epidemic around the world.The manipulation of SARS-CoV-2 is restricted to biosafety level 3 laboratories(BSL-3).In this study,we developed a SARS-CoV-2ΔN-GFP-HiBiT replicon delivery particles(RDPs)encoding a dual reporter gene,GFP-HiBiT,capable of producing both GFP signal and luciferase activities.Through optimal selection of the reporter gene,GFP-HiBiT demonstrated superior stability and convenience for antiviral evaluation.Additionally,we established a RDP infection mouse model by delivering the N gene into K18-hACE2 KI mouse through lentivirus.This mouse model supports RDP replication and can be utilized for in vivo antiviral evaluations.In summary,the RDP system serves as a valuable tool for efficient antiviral screening and studying the gene function of SARS-CoV-2.Importantly,this system can be manipulated in BSL-2 laboratories,decreasing the threshold of experimental requirements.
基金This study was part of the Project of Health and Medical Technology of Zhejiang Province funded by the Health Commission of Zhejiang Province(2020KY536).
文摘A significant number of biosafety level 2(BSL-2)laboratories have been established in many countries for studies of various types of pathogenic agents and other infectious biological materials.The harmonized management of biological risks in such diverse laboratories thus appears as a real challenge.Zhejiang Province in China has taken the initiative to establish a comprehensively integrated laboratory biosafety management system called SINS(Standardization,Informatization,Normalization and Systematization).The SINS model system has been introduced and adopted in 1,721 BSL-2 laboratories in Zhejiang Province,and thus lead to an increase in the number of biosafety committees from 20%to more than 95%from 2007 to 2018,and the number of biosafety laboratory managers who knows biosafety-related laws and regulations increase from 52.7%to 83.7%from 2009 to 2017.Such achievements indicate that the successful implementation of SINS model has increased the effective control of biological risks in BSL-2 laboratories of the Zhejiang Province.SINS model and its main effects on leading the improvement of laboratory biosafety management was presented in this review.The SINS model helps to strengthen laboratory biosafety and thus effectively reduces occurrence of biosafety-related incidences.This model can potentially be used by other regions or countries where harmonized biosafety management system is still under-developing.
