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血清成纤维细胞生长因子21和脂肪酸结合蛋白4检测对急性ST段抬高型心肌梗死患者经皮冠状动脉介入治疗术后心力衰竭的预测价值 被引量:1
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作者 包美珍 韩惠 《中国心血管病研究》 CAS 2024年第5期469-474,共6页
目的探究血清成纤维细胞生长因子21(FGF21)和脂肪酸结合蛋白4(FABP4)检测对急性ST段抬高型心肌梗死(STEMI)患者经皮冠状动脉介入治疗(PCI)术后心力衰竭的预测价值。方法选取2020年9月至2022年9月内蒙古医科大学附属医院接诊的113例STEM... 目的探究血清成纤维细胞生长因子21(FGF21)和脂肪酸结合蛋白4(FABP4)检测对急性ST段抬高型心肌梗死(STEMI)患者经皮冠状动脉介入治疗(PCI)术后心力衰竭的预测价值。方法选取2020年9月至2022年9月内蒙古医科大学附属医院接诊的113例STEMI患者为研究对象,依据PCI术后1年是否发生心力衰竭(心衰),将其分为心衰组(n=32)和非心衰组(n=81)。应用ELISA法测定血清FGF21、FABP4表达水平,比较两组血清FGF21、FABP4水平,多因素logistic回归分析影响STEMI患者PCI术后发生心力衰竭的相关因素,ROC曲线评估血清FGF21、FABP4水平对STEMI患者PCI术后心力衰竭发生的预测价值。结果心衰组心率次数、C反应蛋白(CRP)、心肌肌钙蛋白(cTnI)、N末端B型利钠肽原(BNP)、利尿剂使用比例均显著高于非心衰组,左心室射血分数(LVEF)显著低于非心衰组(P<0.05)。心衰组血清FGF21、FABP4表达水平均明显高于非心衰组[(228.37±33.07)ng/L比(185.68±25.52)ng/L、(34.26±5.51)ng/ml比(26.87±4.67)ng/ml,t=7.345、7.195,P<0.05]。血清FGF21(95%CI 1.371~8.191)、FABP4(95%CI 1.176~4.090)及发病到至导丝通过时间(95%CI 1.058~8.157)是影响STEMI患者PCI术后发生心力衰竭的危险因素(OR>1,P<0.05),LVEF(95%CI 0.473~0.913)是保护因素(OR<1,P<0.05)。血清FGF21、FABP4单独及二者联合预测STEMI患者PCI术后发生心力衰竭的曲线下面积(AUC)分别为0.828、0.856、0.934,二者联合优于单一(Z二者联合-FGF21=1.971、Z二者联合-FABP4=2.417,P=0.048、P=0.015)。结论STEMI患者PCI术后发生心力衰竭血清FGF21、FABP4水平均明显升高,二者联合对STEMI患者PCI术后发生心力衰竭的风险具有更高的预测价值。 展开更多
关键词 心力衰竭 成纤维细胞生长因子21 脂肪酸结合蛋白4 急性st段抬高型心肌梗死
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Ecological Discourse Analysis Based on the Corpus-A Case Study on the Environmental Discourse of 21st Century
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作者 CHEN Yuang ZHI Yong-bi 《Journal of Literature and Art Studies》 2024年第8期693-702,共10页
Language learning materials not only provide language input for learners but also subtly disseminate ideology and influence readers’behavior.This study,based on the framework of ecological discourse analysis,quantita... Language learning materials not only provide language input for learners but also subtly disseminate ideology and influence readers’behavior.This study,based on the framework of ecological discourse analysis,quantitatively analyzes and qualitatively describes the ecological significance of environmental texts in the 21st Century.The aim is to provide references for the compilation of environmental discourse in language learning materials,promote the construction of more ecologically beneficial discourse,and facilitate the widespread dissemination of the ecological philosophy of“Diversity and Harmony,Interaction and Co-existence”among young readers.The initial findings of this study are as follows:In the environmental texts of the publication,a large number of relational processes and action processes are used to objectively represent the natural environment;the discourse tends to be ambiguous and beneficial,generally aligning with the ecological philosophy of“Diversity and Harmony,Interaction and Co-existence.”This can guide readers to correctly understand and face environmental issues,which is conducive to the healthy development of natural ecosystems. 展开更多
关键词 21st Century ecological discourse analysis ecological orientation a corpus-based study
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外周血microRNA‐21和NF‐κB对经皮冠状动脉介入治疗术后患者ST段回落不良的预测价值
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作者 石红霞 任智敏 刘慧慧 《海军医学杂志》 2023年第11期1185-1188,共4页
目的研究外周血microRNA‑21(miR‑21)和核因子κB(NF‑κB)对经皮冠状动脉介入(PCI)治疗的ST段抬高型心肌梗死(STEMI)患者术后ST段回落不良的预测价值。方法以2018年1月至2021年1月于如皋市人民医院进行PCI治疗的150例STEMI患者作为研究对... 目的研究外周血microRNA‑21(miR‑21)和核因子κB(NF‑κB)对经皮冠状动脉介入(PCI)治疗的ST段抬高型心肌梗死(STEMI)患者术后ST段回落不良的预测价值。方法以2018年1月至2021年1月于如皋市人民医院进行PCI治疗的150例STEMI患者作为研究对象,其中PCI术后ST段回落不良组患者44例,ST段回落良好组患者106例,比较术前2组患者的miR‑21和NF‑κB水平以及术中资料之间的差异,分析外周血miR‑21和NF‑κB对PCI术后ST段回落不良的预测价值。结果2组患者的PCI术中病变血管情况、梗死相关动脉、治疗中的替罗非班使用情况、血栓抽吸、植入支架、靶病变血管直径、靶病变血管长度及球囊扩张压力差异无统计学意义(P>0.05);ST段回落不良组术前的miR‑21和NF‑κB水平高于ST段回落良好组(P<0.01)。通过ROC曲线分析发现miR‑21和NF‑κB联合检测预测ST段回落不良的曲线下面积高于单独检测,ST段回落不良患者术前的miR‑21和NF‑κB临界值分别为2.30及1.31 ng/L。结论通过对患者的NF‑κB与miR‑21进行分析,可以对PCI术后ST段回落不良情况进行预测。 展开更多
关键词 心肌梗死 PCI st段回落不良 microRNA‑21 核因子ΚB
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血清miR-1、miR-21水平预测急性ST段抬高型心肌梗死患者介入治疗1年后预后的临床分析
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作者 王勇 《航空航天医学杂志》 2023年第12期1456-1459,共4页
目的 探讨血清微小核糖核酸(miR)-1、miR-21水平预测急性ST段抬高型心肌梗死(STEMI)患者介入治疗1年后预后的价值。方法 回顾性分析,收集2020年03月-2022年03月期间收治的行介入治疗1年后预后不良的40例急性STEMI患者临床资料,纳入预后... 目的 探讨血清微小核糖核酸(miR)-1、miR-21水平预测急性ST段抬高型心肌梗死(STEMI)患者介入治疗1年后预后的价值。方法 回顾性分析,收集2020年03月-2022年03月期间收治的行介入治疗1年后预后不良的40例急性STEMI患者临床资料,纳入预后不良组;另收集同期医院行介入治疗1年后预后良好的40例急性STEMI患者临床资料,纳入预后良好组。所有患者基线资料均完整,包括一般资料、血清指标检测资料,重点分析入院时血清miR-1、miR-21水平对急性STEMI介入治疗1年后预后不良的预测价值。结果 预后良好组与预后不良组的血清心肌肌钙蛋白-I(cTnI)、肌酸激酶同工酶(CK-MB)、miR-1、miR-21水平比较,差异有统计学意义(P<0.05);回归分析结果显示,血清cTnI、CK-MB、miR-1、miR-21的异常表达可能与急性STEMI患者介入治疗1年后预后不良有关,其中血清cTnI、CK-MB、miR-21是急性STEMI患者介入治疗1年后预后不良的风险因子(OR>1,P<0.05),血清miR-1的高表达是急性STEMI患者介入治疗1年后预后不良的保护因子(OR<1,P<0.05);绘制ROC曲线,结果提示血清miR-1、miR-21预测急性STEMI介入治疗1年后预后不良风险的AUC均>0.80。结论 血清miR-1、miR-21水平对急性STEMI患者介入治疗1年后预后不良风险具有一定预测价值。 展开更多
关键词 st段抬高型心肌梗死 微小核糖核酸-1 微小核糖核酸-21 介入治疗 预后
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“21世纪海上丝绸之路”:海上风能的宏观与微观等级区划
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作者 郑崇伟 《哈尔滨工程大学学报》 EI CAS CSCD 北大核心 2024年第7期1258-1265,共8页
以风能宏观战略布局、微观精准选址对合理资源等级区划的迫切需求为牵引,本文采用Delphi法,充分考虑资源特征、环境风险、成本效益,对“21世纪海上丝绸之路”(“海上丝路”)及关键海域的风能展开了常规需求、商业开发、应急供电、用电... 以风能宏观战略布局、微观精准选址对合理资源等级区划的迫切需求为牵引,本文采用Delphi法,充分考虑资源特征、环境风险、成本效益,对“21世纪海上丝绸之路”(“海上丝路”)及关键海域的风能展开了常规需求、商业开发、应急供电、用电量巨大几种情景下的宏观和微观等级区划。研究结果表明:从“海上丝路”的风能宏观等级区划来看,大部分区域的风能属于较丰富区和丰富区(风能等级期望值大于0.5),索马里近海、马纳尔湾、斯里兰卡东南近海、南海大风区为风能等级的相对大值中心。从斯里兰卡海域的风能微观等级区划来看,相对富集区分布于斯里兰卡和印度半岛之间的海峡、斯里兰卡东南部近海(风能等级期望值在0.7以上);贫乏区为印度半岛东西海岸、斯里兰卡东部近海和西南部近海。 展开更多
关键词 21世纪海上丝绸之路 斯里兰卡 风能等级区划 资源特征 环境风险 成本效益 战略布局 精准选址
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血清NRF2和FGF21水平与ST段抬高型心肌梗死患者PCI术后恶性心律失常的相关性 被引量:1
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作者 王敏燕 季俭 郝晓娇 《医学研究与战创伤救治》 CAS 北大核心 2023年第7期755-759,共5页
目的探讨血清核因子E2相关因子2(NRF2)和成纤维细胞生长因子21(FGF21)水平与ST段抬高型心肌梗死(STEMI)患者经皮冠状动脉介入治疗(PCI)术后恶性心律失常(MVA)的相关性研究。方法回顾性分析2019年5月至2021年5月上海健康医学院附属崇明... 目的探讨血清核因子E2相关因子2(NRF2)和成纤维细胞生长因子21(FGF21)水平与ST段抬高型心肌梗死(STEMI)患者经皮冠状动脉介入治疗(PCI)术后恶性心律失常(MVA)的相关性研究。方法回顾性分析2019年5月至2021年5月上海健康医学院附属崇明医院接收因STEMI行PCI术患者150例(STEMI组),以及同期健康体检者150例(对照组)临床资料。采用ELISA法检测血清NRF2、FGF21的水平。采用多因素Logistic回归分析STEMI患者PCI术后发生MVA的影响因素。受试者工作曲线(ROC)评价血清NRF2、FGF-21在STEMI患者PCI术后发生MVA的预测效能。结果相较于对照组,STEMI组患者血清NRF2、FGF21显著降低(P<0.05)。发生MVA组的手术时间时长、术前心功能KllipⅢ级人数、cTnl、CK-MB、UA、Hcy、IL-1β、IL-6、TNF-α水平显著高于未发生MVA组,NRF2、FGF21水平显著低于未发生MVA组(P<0.05)。多因素Logistic回归分析显示,手术时间时长、高水平IL-1β、TNF-α均是STEMI患者PCI术后发生MVA的独立危险因素;高水平NRF2、FGF21均是STEMI患者PCI术后发生MVA的独立保护因素(P<0.05)。ROC结果显示,血清NRF2、FGF21水平及两者联合预测STEMI患者PCI术后发生MVA的AUC分别为0.754、0.760、0.843,其中联合预测AUC显著高于两者单独预测AUC(Z=5.491、5.943,P<0.05)。结论STEMI患者血清NRF2、FGF21水平降低,并可作为STEMI患者PCI术后发生MVA的保护危险因素,对STEMI患者PCI术后发生MVA具有较高的预测价值。 展开更多
关键词 st段抬高型心肌梗死 核因子E2相关因子2 成纤维细胞生长因子21 经皮冠状动脉介入术 预测价值
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EXPERIMENTAL STUDY ON MOXIBUSTION AT ZUSANLI(ST 36) AND LIANGMEN (ST 21) INDUCING HEAT SHOCK PROTEIN 70 (HSP70) EXPRESSION TO RESIST OXIDATIVE INJURY OF GASTRIC MUCOSA 被引量:2
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作者 彭娜 常小荣 +2 位作者 易受乡 彭艳 严洁 《World Journal of Acupuncture-Moxibustion》 2006年第2期22-28,共7页
To observe effect of moxibustion at Zusanli (足三里 ST 36) and Liangmen (梁门 ST 21 ) on expression of heat shock protein 70 (HSP70) in gastric mucosa of the rat of stress ulcer (SU) to explore the mechanism o... To observe effect of moxibustion at Zusanli (足三里 ST 36) and Liangmen (梁门 ST 21 ) on expression of heat shock protein 70 (HSP70) in gastric mucosa of the rat of stress ulcer (SU) to explore the mechanism of moxibustion in resisting oxidative injury of the gastric mucosa. Methods: Sixty SD rats were evenly randomized into 4 groups, a blank group, a model group, an acupoint moxibustion group and a non-acupoint moxibustion group. Water restraint stress (WRS) method was used to make stress gastric ulcer rat model. The ulcerative index (Ul) of gastric mucosa was evaluated by using GUTH method, the gastric mu- cosa blood flux (GMBF) was detected by a laser Doppler bloodflow monitor, and HSP70 expression and malondialdehyde (MDA) content in the gastric mucosa were determined respectively with immunohistochemical and thiobarbiturate methods. Results: Moxibustion at Zusanli (ST 36) and Liangmen (ST 21 ) significantly de- creased Ul, up-regulated HSP70 expression, increased GMBF, and decreased MDA content in the gastric mucosa in the rat of stress gastric ulcer, with significant differences as compared with the model group and the non-acupoint moxibustion group (P 〈 0.05 or P 〈 0.01 ). Conclusion: Moxibustion at Zusanli (ST 36) and Liangmen (ST 21 ) can induce high expression of HSP70 and decrease MDA content in the gastric mucosa, so as to resist oxidative injury, with relative acupoint specificity. 展开更多
关键词 Moxibustion Zusanli (st 36) liangmen (st 21 Heat Shock Protein 70 Malondialdehyde
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Research as a Veritable Tool for Enhancing Students’ Job Creation in the 21st Century University Education
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作者 Akujieze M.O. 《Journal of International Education and Practice》 2023年第2期14-24,共11页
This study explores the role of research as a tool for enhancing students’job creation in the context of 21st-century university education.The survey research design was the approach used for this investigation.The r... This study explores the role of research as a tool for enhancing students’job creation in the context of 21st-century university education.The survey research design was the approach used for this investigation.The researcher created a questionnaire to collect pertinent data for the study from 200 respondents.The questionnaire was duly validated by 3 experts.The internal reliability of the instrument was examined in this study using Cronbach’s alpha(α).Cronbach’s alpha method is able to determine the correlation between the outcomes of each test item and the test’s total score.Google Forms was used to deliver the survey to the respondents electronically.A statistical package was used to calculate the mean and standard deviation.The study hypotheses were also investigated using inferential statistics(t-test)with 198 degrees of freedom and a 0.05 threshold of significance.To determine whether to accept or reject the null hypothesis,the critical value and estimated t-value are compared.Findings suggest that research-oriented education positively impacts students’ability to create job opportunities in the 21st century.Engaging in research projects fosters critical thinking,creativity,and adaptability among students,enabling them to identify and capitalize on emerging trends and market gaps.Furthermore,the study reveals the significance of university-industry collaborations and mentorship programs in enhancing students’entrepreneurial skills and connecting them with practical resources and networks.The implications of this research are significant for educational policymakers,university administrators,and curriculum developers,as it underscores the importance of incorporating research as an integral component of 21st-century university education. 展开更多
关键词 RESEARCH Veritable tool Job creation 21st century University Education
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Lp(a)联合miRNA-21对NSTE-ACS患者PCI术后冠状动脉再狭窄的预测价值分析
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作者 李艳 王猛 程诚 《心血管病防治知识(学术版)》 2023年第20期29-31,共3页
目的探讨脂蛋白a[LP(a)]、微小核糖核酸-21(miRNA-21)对非ST段抬高型急性冠脉综合征(NSTE-ACS)患者经皮冠状动脉介入(PCI)术后冠状动脉再狭窄的预测价值。方法回顾性选取2020年1月至2022年5月至该院接受PCI治疗的195例NSTE-ACS患者,根... 目的探讨脂蛋白a[LP(a)]、微小核糖核酸-21(miRNA-21)对非ST段抬高型急性冠脉综合征(NSTE-ACS)患者经皮冠状动脉介入(PCI)术后冠状动脉再狭窄的预测价值。方法回顾性选取2020年1月至2022年5月至该院接受PCI治疗的195例NSTE-ACS患者,根据患者PCI术后1年内是否发生冠状动脉再狭窄进行分组,即狭窄组(38例)和对照组(157例)。收集入组患者一般资料及实验室检查结果,使用多因素Logistic逐步回归分析PCI术后冠状动脉再狭窄的危险因素,结合受试者工作特征(ROC)曲线评估其预测价值。结果狭窄组Lp(a)水平、miRNA-21表达量均显著高于对照组(P<0.05)。Logistic回归分析结果显示,Lp(a)、miRNA-21升高是PCI术后发生冠状动脉再狭窄的独立危险因素(P<0.05)。ROC曲线分析结果显示,Lp(a)、miRNA-21预测PCI术后冠状动脉再狭窄的曲线下面积(AUC)分别为0.878、0.729,二者联合预测的AUC为0.884,显著高于单独指标(Z=9.081、4.692,P=0.000、0.000),敏感度为71.05%,特异度为97.45%。结论Lp(a)、miRNA-21与NSTE-ACS患者PCI术后冠状动脉再狭窄密切相关,二者联合检测可有效预测PCI术后冠状动脉再狭窄。 展开更多
关键词 脂蛋白A 微小核糖核酸-21 st段抬高型急性冠脉综合征 经皮冠状动脉介入 冠状动脉再狭窄
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INTERGROWTH-21^(st)标准评价胎儿宫内生长受限的临床价值 被引量:9
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作者 朱晨 任芸芸 吴江南 《复旦学报(医学版)》 CAS CSCD 北大核心 2017年第3期307-311,325,共6页
目的探讨INTERGROWTH-21^(st)标准评价胎儿宫内生长受限的应用价值。方法本研究为前瞻性队列研究。收集2015年3月至2016年3月在复旦大学附属妇产科医院建卡,进行常规产前检查并入院分娩的单胎妊娠孕妇。所有入选病例孕28周之后入组,每4... 目的探讨INTERGROWTH-21^(st)标准评价胎儿宫内生长受限的应用价值。方法本研究为前瞻性队列研究。收集2015年3月至2016年3月在复旦大学附属妇产科医院建卡,进行常规产前检查并入院分娩的单胎妊娠孕妇。所有入选病例孕28周之后入组,每4周做一次产前超声生长测量,直至分娩前。胎儿生长径线以INTERGROWTH-21^(st)标准计算Z-score值。分娩后随访新生儿体重等相关信息。应用Logistic回归方程建立预测公式。最后将新公式与Hadlock法预测IUGR的准确性进行对比。结果共收集符合入组条件的孕妇834例。排除失访143例后,得到对照组565例和IUGR组126例。新预测公式的敏感性、特异性、阳性预测值、阴性预测值、假阳性率、假阴性率分别为:88.9%、85.4%、57.7%、97.2%、14.6%、11.1%。Hadlock法预测的敏感性、特异性、阳性预测值、阴性预测值、假阳性率、假阴性率分别为:81.7%、82.7%、51.2%、95.3%、17.3%、18.3%。结论以INTERGROWTH-21^(st)标准计算Z-score建立新公式预测胎儿宫内生长受限的方法,与传统的Hadlock预测法相比,改善了产前诊断的敏感性和特异度,减少了假阳性和假阴性的病例,全面提高了超声预测胎儿宫内生长受限的准确性。 展开更多
关键词 INTERGROWTH-21st Z-SCORE 胎儿宫内生长受限 超声生长测量 晚孕期
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酪氨酸激酶抑制剂STI571与P21^(WAF)基因克隆治疗慢性粒细胞白血病的实验研究
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作者 王玮 孙秉中 +4 位作者 刘新平 冯琦 尚振川 曹云新 药立波 《中国实验血液学杂志》 CAS CSCD 2004年第6期737-742,共6页
本研究探讨酪氨酸激酶抑制剂STI5 71和P2 1WAF基因克隆对慢性粒细胞白血病急变K5 62细胞株的细胞增殖、细胞周期及凋亡等的治疗作用。RT PCR扩增P2 1WAF基因 ,胶纯化回收后连接到T载体测序 ,序列正确后构建P2 1 pcDNA3 .1载体 ,将P2 1 p... 本研究探讨酪氨酸激酶抑制剂STI5 71和P2 1WAF基因克隆对慢性粒细胞白血病急变K5 62细胞株的细胞增殖、细胞周期及凋亡等的治疗作用。RT PCR扩增P2 1WAF基因 ,胶纯化回收后连接到T载体测序 ,序列正确后构建P2 1 pcDNA3 .1载体 ,将P2 1 pcDNA3 .1与空载体分别以脂质体转染入P2 1蛋白表达缺如的K5 62细胞 ,经筛选得到G4 18抗性的K5 62细胞株 ,Westernblot证实转染后有P2 1蛋白表达 ,MTT法检测细胞存活率 ,流式细胞仪检测细胞周期和凋亡。结果表明 :表达外源性P2 1蛋白的P2 1 pcDNA3 .1 K5 62细胞株生长速度明显慢于对照K5 62细胞株 ,流式细胞仪显示G0 /G1期细胞增多 ,MTT法显示P2 1 pcDNA3 .1 K5 62细胞与STI5 71联合应用后 ,与单用STI5 71作用的K5 62细胞相比 ,凋亡细胞比例轻度减少 ,细胞存活率下降较慢。结论 :表达外源P2 1蛋白能抑制K5 62细胞增殖 ,同时对STI5 71的促凋亡作用有轻度抑制 ,并降低K5 62细胞对STI5 71的敏感性。 展开更多
关键词 酪氨酸激酶抑制刑 st1571 P21^WAF基因 基因克隆 慢性粒细胞白血病
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急性非ST段抬高型心肌梗死早期血浆microRNA-21水平检测的临床意义 被引量:6
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作者 杨捍卫 唐忠志 郝谦 《内科急危重症杂志》 2020年第1期44-46,共3页
目的:探讨血浆缩小RNA-21(microRNA-21,miRNA-21)水平对于急性非ST段抬高型心肌梗死(NSTEMI)的早期诊断价值。方法:选取行冠状动脉造影确诊NSTEMI患者45例及不稳定型心绞痛(UAP)患者53例。另选取同期健康体检者30例作为对照组。检测3组... 目的:探讨血浆缩小RNA-21(microRNA-21,miRNA-21)水平对于急性非ST段抬高型心肌梗死(NSTEMI)的早期诊断价值。方法:选取行冠状动脉造影确诊NSTEMI患者45例及不稳定型心绞痛(UAP)患者53例。另选取同期健康体检者30例作为对照组。检测3组血浆肌钙蛋白T(c Tn T)及肌酸磷酸同工酶(CK-MB)水平,通过定量逆转录聚合酶链反应(qRT-PCR)检测血浆miRNA-21表达水平。采用ROC曲线分析各指标对NSTEMI的诊断价值。结果:①NSTEMI组和UAP组循环miRNA-21、c Tn T、CK-MB水平高于对照组(均P <0. 01);且NSTEMI组循环miRNA-21、c Tn T水平高于UAP组(均P <0. 01);②相关性分析显示,NSTEMI组和UAP组患者血浆miRNA-21与c Tn T、CK-MB水平均呈正相关(r=0. 634,P=0. 000;r=0. 306,P=0. 002);③miRNA-21的ROC曲线下面积(AUC)为0. 946(95%CI:0. 900~0. 993,P=0. 000),当miRNA-21截断值为7. 12时,其灵敏度为91. 11%,特异性为94. 34%;c Tn T的AUC为0. 774(95%CI:0. 683~0. 865,P=0. 000),当c Tn T截断值为0. 82ng/m L时,其灵敏度为82. 22%,特异性为64. 15%;CK-MB的AUC值为0. 588(95%CI:0. 475~0. 701,P=0. 000)。结论:血浆miRNA-21对早期诊断NSTEMI有一定的临床意义。 展开更多
关键词 st段抬高型心肌梗死 MICRORNA-21 诊断
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The Status of English as a Lingua Franca in the 21st Century
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作者 李瑞凌 《海外英语》 2011年第14期350-351,共2页
The status of a language as a lingua franca in the 21st century is not determined by its linguistic merits,or the number of people who speak the language,or a country's language policy,but the combined effect of p... The status of a language as a lingua franca in the 21st century is not determined by its linguistic merits,or the number of people who speak the language,or a country's language policy,but the combined effect of political,economic and cultural forces.English as a future lingua franca has three characteristics:the status of official language in several countries;a priority in countries' foreign-language teaching programme;being widely used all over the world. 展开更多
关键词 ENGLISH stATUS lingua franca the 21st CENTURY
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CASC2和miRNA-21在STEAMI病人急诊PCI前后外周血中的表达变化 被引量:3
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作者 孟庆国 别自东 任法新 《中西医结合心脑血管病杂志》 2019年第2期176-179,共4页
目的观察急性ST段抬高型心肌梗死(STEAMI)病人行急诊经皮冠状动脉介入(PCI)治疗前后外周血中长链非编码RNA癌易感性候选基因2(CASC2)及微小RNA-21(miRNA-21)的表达及变化,探讨CASC2及miRNA-21在STEAMI中的临床价值。方法选择2016年12月... 目的观察急性ST段抬高型心肌梗死(STEAMI)病人行急诊经皮冠状动脉介入(PCI)治疗前后外周血中长链非编码RNA癌易感性候选基因2(CASC2)及微小RNA-21(miRNA-21)的表达及变化,探讨CASC2及miRNA-21在STEAMI中的临床价值。方法选择2016年12月—2017年5月青岛大学附属威海市中心医院心内科住院行急诊PCI的STEAMI病人55例为STEAMI组,排除STEAMI的32名健康者为健康对照组。根据行PCI后是否发生缺血再灌注损伤(MIRI)将STEAMI组分为MIRI组(13例)及非MIRI组(42例),采用实时定量荧光聚合酶链反应技术检测各组外周静脉血中CASC2及miRNA-21表达水平,分析CASC2、miRNA-21与心肌肌钙蛋白T(cTnT)和超敏C反应蛋白(hs-CRP)的相关性。结果 STEAMI组PCI术前及术后CASC2、miRNA-21表达水平均明显高于健康对照组,差异均有统计学意义(P <0.05)。MIRI组CASC2及miRNA-21表达水平均低于非MIRI病人,差异均有统计学意义(P <0.05)。STEAMI病人PCI术后外周血CASC2、miRNA-21与cTnT显著相关(r=0.35,P=0.04;r=0.45,P=0.03),与hs-CRP无相关性(r=0.12,P=0.32;r=0.22,P=0.45);STEAMI病人PCI术后外周血CASC2与miRNA-21呈正相关(r=0.33,P=0.04)。结论 CASC2与miRNA-21可能共同参与了STEAMI心肌MIRI,可预测心肌MIRI。 展开更多
关键词 急性st段抬高型心肌梗死 经皮冠状动脉介入治疗 长链非编码RNA 微小RNA-21 癌易感性候选基因2
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Spatial patterns and driving forces of land use change in China during the early 21st century 被引量:167
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作者 LIU Jiyuan ZHANG Zengxiang +8 位作者 XU Xinliang KUANG Wenhui ZHOU Wancun ZHANG Shuwen LI Rendong YAN Changzhen YU Dongsheng WU Shixin JIANG Nan 《Journal of Geographical Sciences》 SCIE CSCD 2010年第4期483-494,共12页
Land use and land cover change as the core of coupled human-environment systems has become a potential field of land change science (LCS) in the study of global environmental change. Based on remotely sensed data of... Land use and land cover change as the core of coupled human-environment systems has become a potential field of land change science (LCS) in the study of global environmental change. Based on remotely sensed data of land use change with a spatial resolution of 1 km × 1 km on national scale among every 5 years, this paper designed a new dynamic regionalization according to the comprehensive characteristics of land use change including regional differentiation, physical, economic, and macro-policy factors as well. Spatial pattern of land use change and its driving forces were investigated in China in the early 21 st century. To sum up, land use change pattern of this period was characterized by rapid changes in the whole country. Over the agricultural zones, e.g., Huang-Huai-Hai Plain, the southeast coastal areas and Sichuan Basin, a great proportion of fine arable land were engrossed owing to considerable expansion of the built-up and residential areas, resulting in decrease of paddy land area in southern China. The development of oasis agriculture in Northwest China and the reclamation in Northeast China led to a slight increase in arable land area in northern China. Due to the "Grain for Green" policy, forest area was significantly increased in the middle and western developing regions, where the vegetation coverage was substantially enlarged, likewise. This paper argued the main driving forces as the implementation of the strategy on land use and regional development, such as policies of "Western Development", "Revitalization of Northeast", coupled with rapidly economic development during this period. 展开更多
关键词 land use change spatial pattern driving forces the early 21st century China
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稳定表达鸡ST3GALⅠ基因BHK-21细胞株的建立
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作者 陈欢 李明义 高轩 《动物医学进展》 CSCD 北大核心 2013年第3期10-15,共6页
筛选稳定表达鸡ST3GalⅠ基因的BHK-21细胞株,为进一步研究禽流感病毒大规模细胞培养生产疫苗奠定基础。克隆鸡ST3GalⅠ基因并将其插入真核表达质粒pcDNA3.1-EGFP中构建重组质粒pcDNA3.1-EGFP-ST3GaⅠ,鉴定正确的真核表达载体转染BHK-21... 筛选稳定表达鸡ST3GalⅠ基因的BHK-21细胞株,为进一步研究禽流感病毒大规模细胞培养生产疫苗奠定基础。克隆鸡ST3GalⅠ基因并将其插入真核表达质粒pcDNA3.1-EGFP中构建重组质粒pcDNA3.1-EGFP-ST3GaⅠ,鉴定正确的真核表达载体转染BHK-21细胞后,ST3GalⅠ基因在细胞中稳定表达,经G418抗性和RT-PCR筛选鉴定表达阳性的单克隆细胞株。将H9亚型禽流感病毒和H5N1Re-5禽流感病毒按不同比例接种BHK-21-ST3GalⅠ细胞和空白BHK-21细胞,同时设普通胰酶和TPCK-胰酶对照组以检测BHK-21-ST3GalⅠ细胞对H9亚型禽流感病毒和H5N1Re-5禽流感病毒的增殖效果。接毒72h后收获细胞液,常规方法测定血凝素(HA)滴度。通过在BHK-21细胞中稳定表达ST3GalⅠ基因,提高BHK-21细胞表面SAα-2,3Gal受体丰度连续传6代后仍然能检测到其表达,荧光显微镜下能看到绿色荧光,RT-PCR可检出1 029bp的目的条带。结果显示,BHK-21-ST3GalⅠ细胞在接毒量为2%时HA滴度达到1∶256,显著高于空白BHK-21细胞组,表明其更适于流感病毒的增殖,具备生产流感病毒疫苗的潜力。 展开更多
关键词 st3GALⅠ基因 重组质粒 稳定表达 BHK-21细胞
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Rice production in China in the early 21^(st) Century 被引量:8
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《Chinese Rice Research Newsletter》 2000年第2期14-16,共3页
关键词 st Rice production in China in the early 21 CENTURY
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Construction of the 21st Century Maritime Silk Road:Present Situation,Opportunities,Problems and Solutions 被引量:1
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作者 He Fan Zhu He +1 位作者 Zhang Qian Wu Lingwei(译) 《Contemporary Social Sciences》 2018年第3期21-39,共19页
The 21 st Century Maritime Silk Road is an important part of China's new landscape of all-round opening-up,responding to the joint demands of both China and many countries in Southeast Asia,South Asia and Africa.T... The 21 st Century Maritime Silk Road is an important part of China's new landscape of all-round opening-up,responding to the joint demands of both China and many countries in Southeast Asia,South Asia and Africa.This paper examines countries and regions along the 21 st Century Maritime Silk Road and makes an in-depth study of their typical economic conditions,opportunities for cooperation with China,as well as related existing problems.There are opportunities in five areas for China to further cooperate with relevant countries and regions; industrial investment,infrastructure investment,resource development,overseas economic & trade zones and maritime economic development.There are also three outstanding problems; poor coordination among executive agents,insufficient financial support and lack of a sound and complete talent team.This paper offers several suggestions concerning the construction of the 21 st Century Maritime Silk Road to better fit the national conditions and joint demands of China and the countries and regions involved. 展开更多
关键词 Belt and ROAD INITIATIVE the 21st CENTURY MARITIME SILK ROAD infrastructure FINANCIAL support
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SUSTAINABLE DEVELOPMENT FACING CHALLENGESIN 21ST CENTURY
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作者 CHEN Guo-jie(Institute of Mountain Hazards and Environment, the Chinese Academy of Sciences / Ministry of Water Conservancy, Chengdu 610041, P. R. China ) 《Chinese Geographical Science》 SCIE CSCD 2001年第4期3-7,共5页
ABSTRACT: The 21st Century is the time for human seeking harmonizing with environment and carrying out sustainable development strategy. But sustainable development is facing many challenges. They may mainly include t... ABSTRACT: The 21st Century is the time for human seeking harmonizing with environment and carrying out sustainable development strategy. But sustainable development is facing many challenges. They may mainly include the follows: challenge comes from the contradiction between human and the earth, challenge of local benefit conflict to human common goal, challenge of competition to fairness, challenge of unbalanced development in regions and countries, challenge of diversity and challenge of calamity. Challenges are not only pressure, but also the motive force. Challenge exists, the motive force would never stop. Sustainable development was born in challenges, is developing in contradiction and will sustain in conflict. 展开更多
关键词 SUstAINABLE development CONTRADICTION between human and the EARTH 21st CENTURY
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A study on the development trends of tourist marketing policies in the 21^(ST) century
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作者 GUO Ying-zhi (Postdoctoral Station of Applied Economics, Fudan University, Shanghai 200433, China) 《Journal of Geographical Sciences》 SCIE CSCD 2000年第4期89-93,共5页
From the view point of tourist marketing, tourist features of popularity and mass, brand and sense, quality and comfort, level and difference, prevalence and society are studied, and the development trends of tourist ... From the view point of tourist marketing, tourist features of popularity and mass, brand and sense, quality and comfort, level and difference, prevalence and society are studied, and the development trends of tourist marketing policies in the 21st century are put forward in this paper. Such as combining sustainable strategies in tourist development with ecology in green marketing, combining information of tourist trends with network in knowledge marketing, combining innovation in tourist products with diversification in marketing objects, combining levels in marketing goals with integer in marketing decision, combining solid in marketing measures with flexibility in marketing policies, combining conformity in marketing channels with double directions in marketing communication and combining the systems of marketing service with culture in marketing activities. 展开更多
关键词 21st century tourist marketing policy development trend
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