Fosmid library construction and screening for the maize mutant gene Vestigial glume 1

The maize mutant gene Vestigial glume 1(Vg1) has been fine-mapped to a narrow region by map-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening. The fosmid library of Vg1 consisted of574,000 clones with an average insert size of 36.4 kb, representing 7.9-fold coverage of the maize genome. Fosmid stability assays indicated that clones were stable during propagation in the fosmid system. Using Vg1 candidate gene-specific primers, a positive clone was successfully identified. This discovery will pave the way for identifying the function of Vg1 in maize development.

Construction and characterization of a cDNA library from human liver tissue with chronic hepatitis B

Objective: To construct a cDNA library from human liver tissue with chronic hepatitis B and check its quality for investigating the expression level of liver tissue infected by hepatitis B virus. This will then be used to find the relevant genes and interesting proteins associated with the development of hepatitis B. Methods: The total RNA from liver tissue with chronic hepa- titis B was extracted and the mRNA was purified using TRIZOL method. Switching mechanism at 5′ end of the RNA transcript (SMART) technique and CDS III/3′ primer were used for first-strand cDNA synthesis. Long distance polymerase chain reaction (LD PCR) was then used to synthesize the double-strand cDNA that was then digested by Sfi I and fractionated by CHROMA SPIN-400 column. The longer than 0.4 kb cDNAs were collected and ligated to λTriplEx2 vector. Then λ phage packaging reaction and library amplification were performed. The qualities of both unamplified and amplified cDNA libraries were strictly checked by conventional titer determination. Fourteen plaques were randomly picked and tested using PCR with universal primers derived from the sequence flanking the vector. Results: The titers of unamplifed and amplified libraries were 1.94×106 pfu/ml and 1.49×109 pfu/ml respectively. The percentages of recombinants from both libraries were 98.15% in unamplified library and 98.76% in amplified library. The lengths of the inserts were 1.23 kb in average, 1?2 kb in 64.29%, and 0.5?1.0 kb in 35.71%. Conclusion: A high quality cDNA library from human liver tissue with chronic hepatitis B was successfully constructed.

Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers

Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a have human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that spe- cifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked im- munosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study suc- cessfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.

An Examination on the Support of Library Information to the Construction of National Research Organizations

The country’s economic development determines the political and cultural status.At the present time,China’s rapid economic development has greatly improved the country’s international prominence.Although China has high economic and political achievements,China’s cultural soft power is still in a weak position.Cultural soft power is of great significance in improving the country’s overall national strength and international competitiveness.Research organizations are an important part of the country’s soft power and are of great significance to the strengthening of national soft power.Research organizations are the“peripheral brain”in the decision-making process of the government.National government agencies need democratic science in making decisions,and the adopted policy methods must have positive and effective outcomes on the people.Therefore,the national government agencies cannot achieve this without the help of research organizations.This resulted in the development and rising construction of research organizations.With the comprehensive development and construction of research organizations,whilst making progress,has also exposed several shortcomings.The construction of China’s research organizations requires various supports;especially the intelligence agencies and access to library information,which has a unique advantage in promoting the construction of research organizations[1].This paper first introduces the definition,classification and characteristics of a research organization.It will also provide a preliminary understanding of research organizations.The paper will then highlight and analyze the problems encountered by the research organization.Finally,through the grasp of the advantages of the library and intelligence agencies,a solution is proposed.

Research on the Construction of Chinese Universities from Intelligent Library to Smart Library

The study aims to discuss the construction and development of university library,and transform the management and operation mode of university library,thereby realizing the transformation and upgrading to smart library.Taking Anhui University of Finance and Economics as the research object,first,based on the literature and data combined with questionnaire design and expert interview,data statistics was made on the development of smart library.Second,analytical hierarchy process(AHP)and big data analysis were applied to complete the construction of the smart library system.Third,based on the data analysis method,the problems in the construction of the smart library were discussed.The results showed that the Chinese smart library started late and developed rapidly after 2012;from information perception to smart service,the construction of smart library system had realized the transformation and upgrading of library construction;the key to solve the construction of smart library was to standardize library construction,realize resource co-construction and sharing,promote technological innovation,and strengthen the construction of smart librarians.To sum up,the construction of smart library is expected to make the library and librarians become the cooperative objects of teaching and scientific research in colleges and universities.

Based on the Construction of University Library and the Evaluation of Space Service Quality

In order to strengthen the construction of university library,promote the sustainable development of university library,and provide a good library space environment for university students,this paper expounds the quality evaluation of university library by establishing the evaluation index of space service quality,and puts forward the ways to improve the service quality of University Library for reference.

Development and construction of China's higher education libraries

Libraries in China's higher education institutions have been developing in keeping pace with the flourishing development of China's higher education. This article aims to make an introduction to the construction of China's higher education libraries, especially the recent three decades' achievements since China's reform and opening-up in 1978. In this article, the authors draw a general picture of the development of libraries in China's higher education institutions, covering such eight aspects as management, types and positioning,organizational structure and personnel, expenditure and buildings, reader service, building and sharing of resources as well as automation system.

Construction and screening of suppression subtractive hybridization library of renal cell carcinoma

Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-2 ( driver), respectiely. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit ( Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F’. All positive clones picked out were digested and some of which were sequenced. Results The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly,2 represented unknown genes and the other 48 derived from 36 known genes. Conclusion The quality of the SSH library of human RCC is reliable and is construction is the basis for further screening differentially expressed genes of RCC. 6 refs,4 figs, 1 tab.

生成式AI视域下智慧图书馆建设的关键路径

[目的/意义]智慧图书馆已经成为当今图书馆学界和业界最热门的主题之一。智慧图书馆建设的理论研究与实践工作已经取得了初步成效,生成式AI也在蓬勃发展的同时参与到各行各业的建设中。因此有必要立足当下发展,放眼远景目标,确定生成式AI参与智慧图书馆建设的策略。[方法/过程]本文从智慧图书馆空间升级、服务打造、业务重组、人才培养、体系建设5个方面阐明了生成式AI视域下智慧图书馆建设的关键路径。[结果/结论]关键路径研究将为生成式AI引入智慧图书馆下一阶段的建设工作提供借鉴与依据。

高校图书馆特色资源库建设方法和策略研究——以南京师范大学图书馆学人文库(校友文库)建设为例

高校图书馆特色资源建设不仅具有本校的学科特色,而且体现了高校图书馆的学术积淀和人文底蕴。高校图书馆加强学人文库(校友文库)的建设具有文化认同、提升学术水平、促进学术研究交流的重要意义。论文在分享南京师范大学图书馆学人文库(校友文库)文献收集和搭建设计校友文库平台虚拟平台的基础上,对高校图书馆文库建设和服务进行了展望,认为文库需要建立共享交流纽带才能充分调动校友捐赠的参与积极性;创新服务管理模式才能更加助力学术带头人获得更多成果;高效整合学术资源才能加深对校友学术资源的挖掘开发。

美国凯斯西储大学图书馆共享空间建设研究及启示

通过网络调研法、文献调研法和对比分析法,从实体空间设置、共享空间提供的资源、共享空间提供的服务这三个方面深入调查了美国凯斯西储大学凯尔文斯密斯图书馆(KSL)基于用户需求的共享空间构建情况;同时在调研国内高校图书馆已有的空间建设实践基础上,借鉴KSL在实体空间、空间资源和空间服务的建设经验来探索并构建满足本校师生需求和适合自身发展的共享空间。

交叉学科文献资源保障体系研究——以云冈学文献资源保障体系为例

交叉学科文献具有边界模糊、难界定等特点。文章以新兴交叉学科云冈学的文献资源保障体系为例,分析阐述了其存在的问题与建设内容,并据此提出交叉学科文献资源保障体系建设策略,即科学分析,广泛搜集文献;确定原则,明确建设目标;联合保障,创新组织形式;搭建平台,开展智慧服务;全面评价,提升服务水平。

公共图书馆文化内涵转型策略与路径研究

新时代实现中国式现代化要求公共文化服务在促进人的全面发展、凝聚人民精神力量、增强国家文化软实力方面发挥更大作用,亦对图书馆文化内涵建设提出了新的要求。在文化内涵建设策略上,图书馆应充分发挥社会教育职能,将阅读推广工作落到实处,面向大众开展公益性、多元化、系统性、专业性、学术性、社会化的文化建设活动。在建设路径上,应充分发挥社区图书馆的职能,将自上而下的文化建设与自下而上的文化建设相结合,积极探索社会力量共建,全面丰富公共图书馆的文化内涵,使新时代的图书馆成为人们心中的精神家园。

文化自信赋能高校图书馆创新发展

文化自信是一个国家、一个民族发展中最基本、最深沉、最持久的力量,坚定文化自信是全面建设社会主义现代化强国、实现中华民族伟大复兴的时代需要。大学是引领社会发展的重要引擎,高校图书馆理应成为文化自信的践行者。文章在分析文化自信内涵及其与高校图书馆价值耦合的基础上,从理念创新、空间布局、资源禀赋、服务生态、书香校园、地域特色文化等方面提出基于文化自信提升的高校图书馆创新发展路径。

高校图书馆口述馆史实证研究——以云南民族大学图书馆为例

文章在回顾国内口述馆史实证研究现状的基础上,以云南民族大学图书馆开展口述馆史资料收集为例,从项目发起原因、工作流程与步骤、社会效益、存在问题等维度探讨口述馆史工作,并提出规范口述馆史工作仪轨、扩大采集对象与范围、重视口述文献开发与利用、鉴证口述史料真伪、关注著作权归属、做好技术与人力保障等改进措施。

公共图书馆场景营销实现路径研究

场景营销是一种综合运用来自场景和用户的信息,以与用户之间建立深层次连接为核心目标的新营销理论,将其引入公共图书馆有利于提升图书馆文化服务水平,加强图书馆品牌建设发展。文章阐述了场景及场景营销的内涵意义,梳理当前图书馆场景营销的研究现状,总结国内外公共文化机构场景营销的两类主体内容和三种模式,借鉴其经验并提出叙事型、体验型和共创型的公共图书馆场景营销实现路径。

公安实践教学中数字化标本库的构建与应用

为推进信息化教学的发展,提出在公安实践教学中建设数字化标本库的构想。以虚拟仿真教学中的毒品数字化标本库构建与教学组织应用为例,探讨公安数字化标本库建设可持续发展的路径和内涵,提出适合公安特色数字化标本库内容和标准化建设的建议,采用资源利用、整合、共建等高效节约办法,注重数字化样本库和数据库同步开发使用的发展趋势。数字化标本库具有线上自学、线上线下教学、终身学习等优势,可启发公安教育智慧教学工作高质量发展。

公共图书馆少儿有声资源自主建设研究

文章总结分析公共图书馆少儿有声资源自主建设工作现状,指出建设中存在的缺乏规划意识、忽视版权问题、制作品质不高、内容重复单一、资源利用率低等不足,并提出公共图书馆可从确立资源建设方向、规避版权风险、打造有声服务品牌、提升资源利用效能等方面开展少儿有声资源自主建设工作。

高等农业机械学课程案例库建设探析

课程案例教学法是以学生为中心,教师为辅助的典型教学方法,有利于提升学生发现问题、分析问题、解决问题的能力。以工科类研究生的高等农业机械学课程为建设对象,探索建设课程相关内容的案例库,提出该课程案例库的建设必要性、介绍案例选择的原则、案例选择的来源等,对课程内容进行重构,列举教师队伍建设、案例库更新机制等案例库建设保障措施。案例库的建设可对提升该课程的教学质量、促成研究生培养目标等提供支撑。