Fosmid library construction and screening for the maize mutant gene Vestigial glume 1

The maize mutant gene Vestigial glume 1(Vg1) has been fine-mapped to a narrow region by map-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening. The fosmid library of Vg1 consisted of574,000 clones with an average insert size of 36.4 kb, representing 7.9-fold coverage of the maize genome. Fosmid stability assays indicated that clones were stable during propagation in the fosmid system. Using Vg1 candidate gene-specific primers, a positive clone was successfully identified. This discovery will pave the way for identifying the function of Vg1 in maize development.

Transforming the academic library for the future

Purpose: To meet the changing needs of academic and specialized users,university and research libraries are transforming their collections,staff,and services. At the UCLA Library,four principles underlie changes that have been made to support teaching,learning,and research by UCLA students,faculty,researchers,and staff. Efforts have focused on building and enhancing research collections,enriching the student experience through expanded teaching and learning services,transforming the Library's role to support the entire research process,and re-conceptualizing the physical and virtual spaces of the Library to create an expansive and flexible scholarly environment. To support and inform these efforts,the Library is building a robust research agenda and deepening opportunities for faculty engagement.These changes challenge the traditional model of library staffing,and the Library has responded by identifying new areas in which leadership is needed and new recruitment tracks for academic appointments. Implementing such a transformative process has many stakeholders and requires broad input,discussion,and committed engagement to ensure its success.Design/methodology/approach: The UCLA Library has developed its strategic planning process over several years building on broad constituent input,unit and individual work plans,and constituent feedback. The process is high level as well as operational in nature and draws on basic metrics to motivate change.Findings: The four principles of the transition have been sustained over a period of years.Implications and limitations: This article is the personal observation of the author drawing on his own leadership experience and informed by the senior management and staff of the UCLA Library.Originality/value: The process and direction of this transition are focused on the UCLA Library and its role in the UCLA academy.

Sample Collection,DNA Extraction,and Library Construction Protocols of the Human Microbiome Studies in the International Human Phenome Project

The human microbiome plays a crucial role in human health.In the past decade,advances in high-throughput sequencing technologies and analytical software have significantly improved our knowledge of the human microbiome.However,most studies concerning the human microbiome did not provide repeatable protocols to guide the sample collection,handling,and processing procedures,which impedes obtaining valid and timely microbial taxonomic and functional results.This protocol provides detailed operation methods of human microbial sample collection,DNA extraction,and library construction for both the amplicon sequencing-based measurements of the microbial samples from the human nasal cavity,oral cavity,and skin,as well as the shotgun metagenomic sequencing-based measurements of the human stool samples among adult par-ticipants.This study intends to develop practical procedure standards to improve the reproducibility of microbiota profiling of human samples.

The influence of Library in the construction of the university academic ecosystem

The force of the development in the university academic ecosystem depends on the academic subject, the discipline, courses, research projects and other key ecological factors. In this paper, it re-examines the influence of the development of the university academic ecosystem and points out how libraries play an important role in the academic ecosystem construction in the perspective of academic ecological development.

Establishing an academic digital library& information system in China: The case of CADLIS

China Academic Digital Library & Information System(CADLIS) was established as a public service system in 1996. Its mission is to improve the resource sharing for China's higher education. The framework of CADLIS is constructed to allow easy interconnection and interoperability. This paper presents an introduction to the portal,academic resources, bibliographic system, service network, digital library technical platform,standards and criteria of CADLIS as well.

Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers

Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a have human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that spe- cifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked im- munosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study suc- cessfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.

Based on the Construction of University Library and the Evaluation of Space Service Quality

In order to strengthen the construction of university library,promote the sustainable development of university library,and provide a good library space environment for university students,this paper expounds the quality evaluation of university library by establishing the evaluation index of space service quality,and puts forward the ways to improve the service quality of University Library for reference.

The plight and Countermeasures brought to the constructions of library literature by effect of college enrollment

This text introduces the concept of local literature and collection range, explains the significance of literature collection and explores building programs of local university library. Currently, university library generally put a strong emphasis on collection of academic literature, but has less attention on literature collections which reflect the situation of university location, or even worse, simply don＇ t launch business in this field. For the university library, it is a great loss of literature information resources, as well as a great regret of library work. First, what is the local literature？ It is literature data which document a region＇ s past and present issues like politics, economy, culture, education, history, geography and other aspects like important characters and events, customs, folklore etc.

Construction and characterization of a cDNA library from human liver tissue with chronic hepatitis B

Objective: To construct a cDNA library from human liver tissue with chronic hepatitis B and check its quality for investigating the expression level of liver tissue infected by hepatitis B virus. This will then be used to find the relevant genes and interesting proteins associated with the development of hepatitis B. Methods: The total RNA from liver tissue with chronic hepa- titis B was extracted and the mRNA was purified using TRIZOL method. Switching mechanism at 5′ end of the RNA transcript (SMART) technique and CDS III/3′ primer were used for first-strand cDNA synthesis. Long distance polymerase chain reaction (LD PCR) was then used to synthesize the double-strand cDNA that was then digested by Sfi I and fractionated by CHROMA SPIN-400 column. The longer than 0.4 kb cDNAs were collected and ligated to λTriplEx2 vector. Then λ phage packaging reaction and library amplification were performed. The qualities of both unamplified and amplified cDNA libraries were strictly checked by conventional titer determination. Fourteen plaques were randomly picked and tested using PCR with universal primers derived from the sequence flanking the vector. Results: The titers of unamplifed and amplified libraries were 1.94×106 pfu/ml and 1.49×109 pfu/ml respectively. The percentages of recombinants from both libraries were 98.15% in unamplified library and 98.76% in amplified library. The lengths of the inserts were 1.23 kb in average, 1?2 kb in 64.29%, and 0.5?1.0 kb in 35.71%. Conclusion: A high quality cDNA library from human liver tissue with chronic hepatitis B was successfully constructed.

5G and smart library construction

The development of fifth-generation mobile communication technology,5G technology,is accelerating.With the ultimate experience and greater capacity,a world of Internet of everything will soon be opened.And the data flow will bring about a smart society with machine learning and artificial intelligence;the library will also enter the era of wisdom.At present,smart libraries at home and abroad have shifted from theoretical discussion to application development.

An Examination on the Support of Library Information to the Construction of National Research Organizations

The country’s economic development determines the political and cultural status.At the present time,China’s rapid economic development has greatly improved the country’s international prominence.Although China has high economic and political achievements,China’s cultural soft power is still in a weak position.Cultural soft power is of great significance in improving the country’s overall national strength and international competitiveness.Research organizations are an important part of the country’s soft power and are of great significance to the strengthening of national soft power.Research organizations are the“peripheral brain”in the decision-making process of the government.National government agencies need democratic science in making decisions,and the adopted policy methods must have positive and effective outcomes on the people.Therefore,the national government agencies cannot achieve this without the help of research organizations.This resulted in the development and rising construction of research organizations.With the comprehensive development and construction of research organizations,whilst making progress,has also exposed several shortcomings.The construction of China’s research organizations requires various supports;especially the intelligence agencies and access to library information,which has a unique advantage in promoting the construction of research organizations[1].This paper first introduces the definition,classification and characteristics of a research organization.It will also provide a preliminary understanding of research organizations.The paper will then highlight and analyze the problems encountered by the research organization.Finally,through the grasp of the advantages of the library and intelligence agencies,a solution is proposed.

An analysis of user interactive learning behavior in academic library information commons in China

This study explores the influencing factors of user interactive learning behavior in academic library information commons,by extending the original Technology Acceptance Model.The hypothesized relationships were tested via structural equation modeling,combining the specific context of academic library information commons.The results show that:a) Perceived usefulness,perceived ease of use,and perceived encouragement are the direct influencing factors;b) Perceived ease of use and perceived encouragement can directly affect perceived usefulness;c) User characteristic,social identity,service content affect user interactive learning behavior via perceived encouragement and perceived ease of use;d) Service ability can affect user interactive learning behavior via perceived encouragement.

Research on the Construction of Chinese Universities from Intelligent Library to Smart Library

The study aims to discuss the construction and development of university library,and transform the management and operation mode of university library,thereby realizing the transformation and upgrading to smart library.Taking Anhui University of Finance and Economics as the research object,first,based on the literature and data combined with questionnaire design and expert interview,data statistics was made on the development of smart library.Second,analytical hierarchy process(AHP)and big data analysis were applied to complete the construction of the smart library system.Third,based on the data analysis method,the problems in the construction of the smart library were discussed.The results showed that the Chinese smart library started late and developed rapidly after 2012;from information perception to smart service,the construction of smart library system had realized the transformation and upgrading of library construction;the key to solve the construction of smart library was to standardize library construction,realize resource co-construction and sharing,promote technological innovation,and strengthen the construction of smart librarians.To sum up,the construction of smart library is expected to make the library and librarians become the cooperative objects of teaching and scientific research in colleges and universities.

Construction and screening of suppression subtractive hybridization library of renal cell carcinoma

Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-2 ( driver), respectiely. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit ( Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F’. All positive clones picked out were digested and some of which were sequenced. Results The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly,2 represented unknown genes and the other 48 derived from 36 known genes. Conclusion The quality of the SSH library of human RCC is reliable and is construction is the basis for further screening differentially expressed genes of RCC. 6 refs,4 figs, 1 tab.

Living Library为高校开展阅读疗法提供新契机

阅读疗法的理论、实践在我国已取得长足发展,但还未得到普及和推广。Living Li-brary作为图书馆新型的服务方式,为阅读疗法常态化机制的建立提供了新契机。该文简要介绍了阅读疗法与Living Library的兴起与现状,分析了高校图书馆传统的阅读疗法存在的不足与高校开展Living Library活动的巨大潜力,从Living Library的主题确定、Living Book的甄选、编目和管理、导读及阅读疗法的常态化机制的建立上阐述了Living Library与阅读疗法的融合。指出Living Library可以克服阅读疗法信息单向流动的弊端,实现了"书"与读者之间的双向交流互动。

类MyLibrary个人数字图书馆构建方案研究

分析在图书馆服务器构建类MyLibrary个人数字图书馆的必要性、意义。从资源对象及组织形式、元数据描述框架、系统主要功能模块及整体实现三个方面论述类MyLibrary个人数字图书馆构建方案。最后提出通过改造和拓展MyLibrary@LANL或Skolr的系统,并参考Refwork、360DOC等系统项目的实现方案。

中外高校图书馆Human Library活动比较研究——以美国阿肯色大学图书馆和上海交通大学图书馆为例

论文详细介绍了阿肯色大学图书馆和上海交通大学图书馆Human Library活动的发展现状,并对二者进行比较分析,总结了高校图书馆开展Human Library活动的经验,并在分析问题的基础上提出了相应的解决策略。

Library2.0背景下体育教授个人数字图书馆的探索

在Web2.0的技术支持下,提出构建体育教授个人数字图书馆的构想,介绍了个人数字图书馆的功能和意义,重点描述了构建体育教授个人数字图书馆的具体模式,最后提出面对机遇与挑战的对策。

My Library系统与高校图书馆的个性化服务

My Library是图书馆个性化信息服务的主要模式,也是网络环境下图书馆服务发展的重要方向。通过对个性化信息服务的内涵、My Library系统的兴起与影响进行阐述的基础上,依据科学合理的性能评价指标体系对高校图书馆的Mylibrary系统在信息资源组织、个性化检索、信息推送等方面进行了分析和评价,并对如何改进高校图书馆的My Library系统提出一些针对性的建议。

A study of the IC^2 Culture Exploring Project of Shanghai Jiao Tong University (SJTU) Library

The paper analyzes the environments confronted with academic libraries and the evolving characteristics of Shanghai Jiao Tong University that give shape to the conception and implementation of an IC2Culture Exploring Project. This innovative model of SJTU Library operation is not only in concert with the strategic goals of SJTU but also with two other conspicuous developmental trends in the larger academic library circle;namely, 1) a trend toward the deepening of library service support in academic arena on the one hand and 2) the trend of diversifying library outreach services on the other hand.Based on our first-hand involvement in all phases and aspects of this project and its subsequent review and analysis undertakings, this paper expatiates upon the IC2Culture Exploring Project of SJTU Library in terms of its vision, its specific mission objectives, its program design, its unique characteristics, its launching process, its salient case studies, its initial results and its strategies for a sustainable development in the future.