Pseudo-allergic reactions(PARs)widely occur upon application of drugs or functional foods.Anti-pseudo-allergic ingredients from natural products have attracted much attention.This study aimed to investigate anti-pseud...Pseudo-allergic reactions(PARs)widely occur upon application of drugs or functional foods.Anti-pseudo-allergic ingredients from natural products have attracted much attention.This study aimed to investigate anti-pseudo-allergic compounds in licorice.The anti-pseudo-allergic effect of licorice extract was evaluated in rat basophilic leukemia 2H3(RBL-2H3)cells.Anti-pseudo-allergic compounds were screened by using RBL-2H3 cell extraction and the effects of target components were verified further in RBL-2H3 cells,mouse peritoneal mast cells(MPMCs)and mice.Molecular docking and human MRGPRX2-expressing HEK293T cells(MRGPRX2-HEK293T cells)extraction were performed to determine the potential ligands of MAS-related G protein-coupled receptor-X2(MRGPRX2),a pivotal target for PARs.Glycyrrhizic acid(GA)and licorice chalcone A(LA)were screened and shown to inhibit Compound48/80-induced degranulation and calcium influx in RBL-2H3 cells.GA and LA also inhibited degranulation in MPMCs and increase of histamine and TNF-a in mice.LA could bind to MRGPRX2,as determined by molecular docking and MRGPRX2-HEK293T cell extraction.Our study provides a strong rationale for using GA and LA as novel treatment options for PARs.LA is a potential ligand of MRGPRX2.展开更多
基金This work was supported by the National Natural Scientific Foundation of China(No.81903788 and 81900780).
文摘Pseudo-allergic reactions(PARs)widely occur upon application of drugs or functional foods.Anti-pseudo-allergic ingredients from natural products have attracted much attention.This study aimed to investigate anti-pseudo-allergic compounds in licorice.The anti-pseudo-allergic effect of licorice extract was evaluated in rat basophilic leukemia 2H3(RBL-2H3)cells.Anti-pseudo-allergic compounds were screened by using RBL-2H3 cell extraction and the effects of target components were verified further in RBL-2H3 cells,mouse peritoneal mast cells(MPMCs)and mice.Molecular docking and human MRGPRX2-expressing HEK293T cells(MRGPRX2-HEK293T cells)extraction were performed to determine the potential ligands of MAS-related G protein-coupled receptor-X2(MRGPRX2),a pivotal target for PARs.Glycyrrhizic acid(GA)and licorice chalcone A(LA)were screened and shown to inhibit Compound48/80-induced degranulation and calcium influx in RBL-2H3 cells.GA and LA also inhibited degranulation in MPMCs and increase of histamine and TNF-a in mice.LA could bind to MRGPRX2,as determined by molecular docking and MRGPRX2-HEK293T cell extraction.Our study provides a strong rationale for using GA and LA as novel treatment options for PARs.LA is a potential ligand of MRGPRX2.
