Strategies for insect population control are currently targeting chemical communication at the molecular level. The diamondback moth Plutella xylostella represents one of the most serious pests in agriculture, however...Strategies for insect population control are currently targeting chemical communication at the molecular level. The diamondback moth Plutella xylostella represents one of the most serious pests in agriculture, however detailed information on the proteins mediating olfaction in this species is still poor. This species is endowed with a repertoire of a large number of olfactory receptors and odorant binding proteins(OBPs). As a contribution to map the specificities of these chemical sensors in the moth and eventually unrave l the complexity of chemodetection, we have measured the affinities of three selected OBPs to a series of potential odorants. Three proteins are highly divergent in their amino acid sequences and show markedly different expression profiles. In fact, PxylOBP3 is exclusively expressed in the antennae of both sexes, PxylOBP9 is male specific and present only in antennae and reproductive organs, while PxylOBP19, an unusual OBP with nine cysteines, is ubiquitously present in all the organs examined. Such expression pattern suggests that the last two proteins may be involved in non-chemosensory functions. Despite such differences, the three OBPs exhibit similar binding spectra, together with high selectivity. Among the 26 natural compounds tested, only two proved to be good ligands, retinol and coniferyl aldehyde. This second compound is particularly interesting being part of the chemical pathway leading to regeneration of lignin, one of the defense strategies of the plant against insect attack, and might find applications as a repellent for P. xylostella and other pests.展开更多
Low carbon landscape development is an emerging approach of landscape design,which aims to promote sustainable development,and reduce energy consumption and environmental impact by reducing carbon emissions.As an impo...Low carbon landscape development is an emerging approach of landscape design,which aims to promote sustainable development,and reduce energy consumption and environmental impact by reducing carbon emissions.As an important component of low carbon landscape construction,the construction of urban pocket park system based on mini-parks plays a crucial role in improving the urban greening level,increasing the urban green space,alleviating the urban pressure,improving the living and healthy environment of urban residents,and promoting the urban ecological balance.Taking Chaowai district of Beijing as an example,this paper calculates the ecological value of trees including energy contribution,carbon sequestration,ability to improve rainfall runoff and aesthetic value by collecting the information of trees and the plot on which the trees are located through the i-Tree platform,which provides important reference value for the urban pocket park system planning to create low carbon comprehensive benefits.展开更多
基金supported by the National Natural Science Foundation of China (31230062 and 31321004)the Beijing Natural Science Foundation of China (6132028)the State Key Laboratory for Biology of Plant Diseases and Insect Pests, Chinese Academy of Agricultural Sciences (SKLOF201502)
文摘Strategies for insect population control are currently targeting chemical communication at the molecular level. The diamondback moth Plutella xylostella represents one of the most serious pests in agriculture, however detailed information on the proteins mediating olfaction in this species is still poor. This species is endowed with a repertoire of a large number of olfactory receptors and odorant binding proteins(OBPs). As a contribution to map the specificities of these chemical sensors in the moth and eventually unrave l the complexity of chemodetection, we have measured the affinities of three selected OBPs to a series of potential odorants. Three proteins are highly divergent in their amino acid sequences and show markedly different expression profiles. In fact, PxylOBP3 is exclusively expressed in the antennae of both sexes, PxylOBP9 is male specific and present only in antennae and reproductive organs, while PxylOBP19, an unusual OBP with nine cysteines, is ubiquitously present in all the organs examined. Such expression pattern suggests that the last two proteins may be involved in non-chemosensory functions. Despite such differences, the three OBPs exhibit similar binding spectra, together with high selectivity. Among the 26 natural compounds tested, only two proved to be good ligands, retinol and coniferyl aldehyde. This second compound is particularly interesting being part of the chemical pathway leading to regeneration of lignin, one of the defense strategies of the plant against insect attack, and might find applications as a repellent for P. xylostella and other pests.
基金Sponsored by General Project of Natural Science Foundation of Beijing City(8202017)Youth Talent Support Program of 2018 Beijing Municipal University Academic Human Resources Development(PXM2018_014212_000043).
文摘Low carbon landscape development is an emerging approach of landscape design,which aims to promote sustainable development,and reduce energy consumption and environmental impact by reducing carbon emissions.As an important component of low carbon landscape construction,the construction of urban pocket park system based on mini-parks plays a crucial role in improving the urban greening level,increasing the urban green space,alleviating the urban pressure,improving the living and healthy environment of urban residents,and promoting the urban ecological balance.Taking Chaowai district of Beijing as an example,this paper calculates the ecological value of trees including energy contribution,carbon sequestration,ability to improve rainfall runoff and aesthetic value by collecting the information of trees and the plot on which the trees are located through the i-Tree platform,which provides important reference value for the urban pocket park system planning to create low carbon comprehensive benefits.