OBJECTIVE: To study the mechanism of Dangfei Liganning capsule(当飞利肝宁胶囊) in the treatment of rats with metabolic associated fatty liver disease(MAFLD). METHODS: Totally 48 specific pathogen free SpragueDawley ma...OBJECTIVE: To study the mechanism of Dangfei Liganning capsule(当飞利肝宁胶囊) in the treatment of rats with metabolic associated fatty liver disease(MAFLD). METHODS: Totally 48 specific pathogen free SpragueDawley male rats were randomly divided into normal Group, model group, Dangfei Liganning high, moderate, and low-dose groups and Essentiale group which were fed with high fat diet for 8 weeks, and gavage and molding were carried out simultaneously. Dangfei Liganning high, middle and low-dose group were given 0.27, 0.135 and 0.0675 g·kg-1·d-1 respectively by gavage, Essentiale group was given 0.123 g·kg-1·d-1 by gavage, the same amount of distilled water was given by gavage in the normal group and the model group. The rats were weighed at the 0th week, 2nd week, 4th week, 6th week and 8th weekend respectively. The rats were sacrificed at the end of the 8th week. Serum levels of alanine aminotransferase(ALT), alanine aminotransferase(AST),triglyceride(TG), total cholesterol(CHO), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein (LDL-C), total protein(TP), albumin(Alb), globulin(GLB), total bilirubin(TBIL), direct bilirubin(DBIL), tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) were measured. The levels of liver tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and liver pathology [hematoxylin and eosin(HE) staining, oil red O staining] were detected. The expression levels of liver X receptor α(LXRα), steroid regulatory element binding protein-1(SREBP-1) and fatty acid synthase(FAS) were detected by immunohistochemistry, Western blot and reverse transcription-polymerase chain reaction reverse transcription-polymerase chain reaction. RESULTS: From the beginning to the 8th week, the growth rate of body weight in the Dangfei Liganning highdose group was slower than all other groups. There was no significant difference in ALB level in all groups(P > 0.05). Compared with the model group, the levels of ALT, AST, LDL-C, TG, CHO, TP, GLB, TBIL, DBIL, IL-6, TNF-α were significantly decreased and HDL-C were significantly increased in Dangfei Liganning high-dose group(P < 0.01, < 0.05). HE and oil red O staining showed that the fatty lesions in rat liver were alleviated, while the expressions of LXRα, SREBP-1, FAS m RNA and protein were significantly decreased(P < 0.01). CONCLUSIONS: Dangfei Liganning capsule can slow down the increase of body weight of MAFLD rats, reduce the levels of transaminase, Lipid and inflammatory factors in MAFLD rats, promote the synthesis of liver protein and bile metabolism, and improve the liver fatty lesion of MAFLD rats, among which the Dangfei Liganning highdose group is more effective. The mechanism of action may be through blocking LXR-SREBP-1-FAS signal pathway.展开更多
Objectives-The aim of this retrospective multicentric study was to assess the complications of the Longo technique for the treatment of haemorrhoidal disease. Methods-From March 1999 to April 2003, 550 patients underw...Objectives-The aim of this retrospective multicentric study was to assess the complications of the Longo technique for the treatment of haemorrhoidal disease. Methods-From March 1999 to April 2003, 550 patients underwent a stapled hemorrhoidectomy following Longo’s technique in 12 surgical units in the Rhne-Alpes Region. The operative indications were the same as for conventional hemorrhoidectomy. Complications were divided into early or late complications depending on whether they occurred before or after the 7 th day. For each patient, the most serious complication was retained for analysis. Results-One hundred and five patients (19%), mean age 51 years, experienced complications. The early complications were bleeding (1.8%), severe anal pain (2.3%), urinary retention (0.9%) and sepsis (0.5%). Late complicationswere chronic anal pain (1.6%), suture dehiscence (1.6%), anal stricture (1.6%) , anal fissure (0.9%), ex- ternal thrombosis (0.9%), fistulae and intramural abscesses (0.9%), anal incontinence (0.3%), haemorrhoidal disease symptoms persistence or recurrence (3.2%). Strictures were successfully dilated, fissures were treated by sphincterotomy, external thromboses were excised and fistulae were laid open. Most of the recurrences were treated with the Milligan-Morgan hemorroidectomy technique. Conclusion-Complications may occur after stapled hemorrhoidopexy, some are particularly serious, especially bleeding and sepsis.展开更多
During the recent decade, many scientists have found cytochrome C3 only from very few organisms and only the intensive studies on the biochemical and physiological functions of cytochrome C3 from Desulfovibrio have be...During the recent decade, many scientists have found cytochrome C3 only from very few organisms and only the intensive studies on the biochemical and physiological functions of cytochrome C3 from Desulfovibrio have been carried out. However, in our nitrogen fixation investigation made on the natural electron transfer carriers in Rhodo-展开更多
In our previous paper, we reported the polarographic catalytic waves of europium and ytterbium and the adsorptive-complex wave of scandium, and in the present note, the polarographic adsorptive-complex waves of yttriu...In our previous paper, we reported the polarographic catalytic waves of europium and ytterbium and the adsorptive-complex wave of scandium, and in the present note, the polarographic adsorptive-complex waves of yttrium, terbium lanthanum, samarium and gadolinium will be reported briefly.展开更多
Bis （1-diethylphosphino-2-diphenylphosphino ethane）-bis （dinitrogen） molybdenum （O）, Mo（N2）2 （Ph2POH2CH2PEt2）2, is a new dinitrogen complex synthesized by Chen Jiabi et al.
The Chinese Mainland deemed homosexuality both a crime and a mental illness well into the 1990s.Gays were prosecuted under "Hooligan"laws and branded psychologically damaged by the Chinese Psychiatric Associ...The Chinese Mainland deemed homosexuality both a crime and a mental illness well into the 1990s.Gays were prosecuted under "Hooligan"laws and branded psychologically damaged by the Chinese Psychiatric Association.The Chinese government repealed its Hooligan laws in 1997,which in turn was seen to denote the decriminalization of homosexuality.A few years later,in 2001,the Chinese Psychiatric Association removed homosexuality from its list of disorders.展开更多
基金Supported by Capital Health Development Research Project:Assessment of the Efficacy of BIEJIAJIANWAN Pill in Patients with Chronic Hepatitis B Cirrhosis/Fibrosis (CD2018-2-2173)Beijing Municipal Administration of Hospitals Incubating Program:Clinical Observation on the Treatment of Nonalcoholic Fatty Liver Disease by Invigorating the Spleen,Soothing the Liver,Activating Blood Circulation and Resolving Phlegm (PZ2019011)。
文摘OBJECTIVE: To study the mechanism of Dangfei Liganning capsule(当飞利肝宁胶囊) in the treatment of rats with metabolic associated fatty liver disease(MAFLD). METHODS: Totally 48 specific pathogen free SpragueDawley male rats were randomly divided into normal Group, model group, Dangfei Liganning high, moderate, and low-dose groups and Essentiale group which were fed with high fat diet for 8 weeks, and gavage and molding were carried out simultaneously. Dangfei Liganning high, middle and low-dose group were given 0.27, 0.135 and 0.0675 g·kg-1·d-1 respectively by gavage, Essentiale group was given 0.123 g·kg-1·d-1 by gavage, the same amount of distilled water was given by gavage in the normal group and the model group. The rats were weighed at the 0th week, 2nd week, 4th week, 6th week and 8th weekend respectively. The rats were sacrificed at the end of the 8th week. Serum levels of alanine aminotransferase(ALT), alanine aminotransferase(AST),triglyceride(TG), total cholesterol(CHO), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein (LDL-C), total protein(TP), albumin(Alb), globulin(GLB), total bilirubin(TBIL), direct bilirubin(DBIL), tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) were measured. The levels of liver tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and liver pathology [hematoxylin and eosin(HE) staining, oil red O staining] were detected. The expression levels of liver X receptor α(LXRα), steroid regulatory element binding protein-1(SREBP-1) and fatty acid synthase(FAS) were detected by immunohistochemistry, Western blot and reverse transcription-polymerase chain reaction reverse transcription-polymerase chain reaction. RESULTS: From the beginning to the 8th week, the growth rate of body weight in the Dangfei Liganning highdose group was slower than all other groups. There was no significant difference in ALB level in all groups(P > 0.05). Compared with the model group, the levels of ALT, AST, LDL-C, TG, CHO, TP, GLB, TBIL, DBIL, IL-6, TNF-α were significantly decreased and HDL-C were significantly increased in Dangfei Liganning high-dose group(P < 0.01, < 0.05). HE and oil red O staining showed that the fatty lesions in rat liver were alleviated, while the expressions of LXRα, SREBP-1, FAS m RNA and protein were significantly decreased(P < 0.01). CONCLUSIONS: Dangfei Liganning capsule can slow down the increase of body weight of MAFLD rats, reduce the levels of transaminase, Lipid and inflammatory factors in MAFLD rats, promote the synthesis of liver protein and bile metabolism, and improve the liver fatty lesion of MAFLD rats, among which the Dangfei Liganning highdose group is more effective. The mechanism of action may be through blocking LXR-SREBP-1-FAS signal pathway.
文摘Objectives-The aim of this retrospective multicentric study was to assess the complications of the Longo technique for the treatment of haemorrhoidal disease. Methods-From March 1999 to April 2003, 550 patients underwent a stapled hemorrhoidectomy following Longo’s technique in 12 surgical units in the Rhne-Alpes Region. The operative indications were the same as for conventional hemorrhoidectomy. Complications were divided into early or late complications depending on whether they occurred before or after the 7 th day. For each patient, the most serious complication was retained for analysis. Results-One hundred and five patients (19%), mean age 51 years, experienced complications. The early complications were bleeding (1.8%), severe anal pain (2.3%), urinary retention (0.9%) and sepsis (0.5%). Late complicationswere chronic anal pain (1.6%), suture dehiscence (1.6%), anal stricture (1.6%) , anal fissure (0.9%), ex- ternal thrombosis (0.9%), fistulae and intramural abscesses (0.9%), anal incontinence (0.3%), haemorrhoidal disease symptoms persistence or recurrence (3.2%). Strictures were successfully dilated, fissures were treated by sphincterotomy, external thromboses were excised and fistulae were laid open. Most of the recurrences were treated with the Milligan-Morgan hemorroidectomy technique. Conclusion-Complications may occur after stapled hemorrhoidopexy, some are particularly serious, especially bleeding and sepsis.
文摘During the recent decade, many scientists have found cytochrome C3 only from very few organisms and only the intensive studies on the biochemical and physiological functions of cytochrome C3 from Desulfovibrio have been carried out. However, in our nitrogen fixation investigation made on the natural electron transfer carriers in Rhodo-
文摘In our previous paper, we reported the polarographic catalytic waves of europium and ytterbium and the adsorptive-complex wave of scandium, and in the present note, the polarographic adsorptive-complex waves of yttrium, terbium lanthanum, samarium and gadolinium will be reported briefly.
文摘Bis （1-diethylphosphino-2-diphenylphosphino ethane）-bis （dinitrogen） molybdenum （O）, Mo（N2）2 （Ph2POH2CH2PEt2）2, is a new dinitrogen complex synthesized by Chen Jiabi et al.
文摘The Chinese Mainland deemed homosexuality both a crime and a mental illness well into the 1990s.Gays were prosecuted under "Hooligan"laws and branded psychologically damaged by the Chinese Psychiatric Association.The Chinese government repealed its Hooligan laws in 1997,which in turn was seen to denote the decriminalization of homosexuality.A few years later,in 2001,the Chinese Psychiatric Association removed homosexuality from its list of disorders.
